| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,360,337:1 | +G | 10.5% | coding (829/1161 nt) | PP_3835 ← | two‑component system sensor histidine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,360,337 | 1 | . | G | 10.5% | 133.7 / 12.0 | 38 | coding (829/1161 nt) | PP_3835 | two‑component system sensor histidine kinase |
| Reads supporting (aligned to +/- strand): ref base . (16/18); new base G (2/2); total (18/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01 | |||||||||||
GAAGATGCGCGGCAGTATCTCTGTGGGTATGCCTTCGCCGCTGTCTTCCACCTCGAAGCGCACGAAGCTGCCCTTCAGCCCTTCAACTTCGCCGAACAACTCGACCACATCGACAGTCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGAGCAACACCATCTGCAGTTGCCCGGCATCCACCTCGATCAACGGCAGGTCCGGTTGCAGCAACACCTTCAAGTCAATCGAGCTTGGCAATGCATGTTCAAGCAGGCCACGCGTAGCGTTAAC > NC_002947/4360188‑4360481 | gAAGATGCGCGGCAGTATCTCTGTGGGTATGCCTTCGCCGCTGTCTTCCACCTCGAAGCGCACGAAGCTGCCCTTCAGCCCTTCAACTTCGCCGAACAACTCGACCACATCGACAGTCAGGCTGACGCGGCGATGCCCTTCGATGGCATc > 2:296625/1‑150 (MQ=255) tGCGCGGCAGTATCTCTGTGGGTATGCCTTCGCCGCTGTCTTCCACCTCGAAGCGCACGAAGCTGCCCTTCAGCCCTTCAACTTCGCCGAACAACTCGACCACATGGACAGTCAGGCTGATTCGGCCATGCCCGTCGATGGCATC‑ACggg < 2:1111305/150‑1 (MQ=255) gcgcGGCAGTATCTCTGTGGGTATGCCTTCGCCGCTGTCTTCCACCTCGAAGCGCACGAAGCTGCCCTTCAGCCCTTCAACTTCGCCGAACAACTCGACCACATCGACAGTCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACGGGc < 2:317956/150‑1 (MQ=255) tctcTGTGGGTATGCCTTCGCCGCTGTCTTCCACCTCGAAGCGCACGAAGCTGCCCTTCAGCCCTTCAACTTCGCCGAACAACTCGACCACATCGACAGTCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACgg > 2:770343/1‑137 (MQ=255) tctcTGTGGGTATGCCTTCGCCGCTGTCTTCCACCTCGAAGCGCACGAAGCTGCCCTTCAGCCCTTCAACTTCGCCGAACAACTCGACCACATCGACAGTCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACgg < 1:770343/137‑1 (MQ=255) tgGGTATGCCTTCGCCGCTGTCTTCCACCTCGAAGCGCACGAAGCTGCCCTTCAGCCCTTCAACTTCGCCGAACAACTCGACCACATCGACAGTCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGa < 2:604197/150‑1 (MQ=255) tGCCTTCGCCGCTGTCTTCCACCTCGAAGCGCACGAAGCTGCCCTTCAGCCCTTCAACTTCGCCGAACAACTCGACCACATCGACAGTCAGGCTGATGCCGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGAGCAaca < 1:296625/150‑1 (MQ=255) cTTCGCCGCTGTCTTCCACCTCGAAGCGCACGAAGCTGCCCTTCAGCCCTTCAACTTCGCCGAACAACTCGACCACATCGACAGTCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGa > 1:285447/1‑141 (MQ=255) cTTCGCCGCTGTCTTCCACCTCGAAGCGCACGAAGCTGCCCTTCAGCCCTTCAACTTCGCCGAACAACTCGACCACATCGACAGTCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGa < 2:285447/141‑1 (MQ=255) ttCGCCGCTGTCTTCCACCTCGAAGCGCACGAAGCTGCCCTTCAGCCCTTCAACTTCGCCGAACAACTCGACCACATCGACAGTCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGAGCAACACCAt > 1:272727/1‑150 (MQ=255) ccgcTGTCTTCCACCTCGAAGCGCACGAAGCTGCCCTTCAGCCCTTCAACTTCGCCGAACAACTCGACCACATCGACAGTCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGAGCAACACCATCTGc > 2:1217102/1‑150 (MQ=255) cgcTGTCTTCCACCTCGAAGCGCACGAAGCTGCCCTTCAGCCCTTCAACTTCGCCGAACAACTCGACCACATCGACAGTCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGAGCAACACCATCTGCa > 1:413163/1‑150 (MQ=255) cTGTCTTCCACCTCGAAGCGCACGAAGCTGCCCTTCAGCCCTTCAACTTCGCCGAACAACTCGACCACATCGACAGTCAGGCTGATGCGGCCATGCCCGT‑GATGCCATCGACGGGCAtgg > 1:295075/1‑118 (MQ=255) cTGTCTTCCACCTCGAAGCGCACGAAGCTGCCCTTCAGCCCTTCAACTTCGCCGAACAACTCGACCACATCGACAGTCAGGCTGATGCGGCCATGCCCGT‑GATGCCATCGACGGGCAtgg < 2:295075/120‑3 (MQ=255) cTCGAAGCGCACGAAGCTGCCCTTCAGCCCTTCAACTTCGCCGAACAACTCGACCACATCGACAGTCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGAGCAACACCATCTGCAGTTGCCCGGCATc < 2:1085881/150‑1 (MQ=255) gcgcACGAAGCTGCCCTTCAGCCCTTCAACTTCGCCGAACAACTCGACCACATCGACAGTCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGAGCAACACCATCTGCAGTTGCCCGGCATCCACCTc < 1:1217102/150‑1 (MQ=255) ccttcagcccttcaACTTCGCCGAACAACTCGACCACATCGACAGTCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGAGCAACACCATCTGCAGTTGCCCGGCATCCACCTCGATCAACGGCAGGt > 1:94601/1‑150 (MQ=255) ccttcagcccttcaACTTCGCCGAACAACTCGACCACATCGACAGTCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGAGCAACACCATCTGCAGTTGCCCGGCATCCACCTCGATCAACGGCAGGt < 2:671302/150‑1 (MQ=255) cttcagcccttcaACTTCGCCGAACAACTCGACCACATCGACAGTCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGAGCAACACCATCTGCAGTTGCCCGGCATCCACCTCGATCAACGGCAGGTc > 2:1091222/1‑150 (MQ=255) tcaACTTCGCCGAACAACTCGACCACATCGACAGTCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGAGCAACACCATCTGCAGTTGccc > 2:1005362/1‑113 (MQ=255) tcaACTTCGCCGAACAACTCGACCACATCGACAGTCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGAGCAACACCATCTGCAGTTGccc < 1:1005362/113‑1 (MQ=255) cGCCGAACAACTCGACCACATCGACAGTCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGAGCAACACCATCTGCAGTTGCCCGGCATCCACCTCGATCAACGGCAGGTCCGGTTGCAGCAACACCt < 2:94601/150‑1 (MQ=255) aacTCGACCACATCGACAGTCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGAGCAACACCATCTGCAGTTGCCCGGCATCCACCTCGATCAACGGCAGGTCCGGTTGCAGCAACACCTTCaccgca > 2:1236639/1‑145 (MQ=255) cTCGACCACATCGACAGTCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGAGCAACACCATCTGCAGTTGCCCGGCATCCACCTCGATCAACGGCAGGTCCGGTTGCAGCAACAcc > 2:331902/1‑139 (MQ=255) cTCGACCACATCGACAGTCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGAGCAACACCATCTGCAGTTGCCCGGCATCCACCTCGATCAACGGCAGGTCCGGTTGCAGCAACAcc < 1:331902/139‑1 (MQ=255) tCGACCACATCGACAGTCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGAGCAACACCATCTGCAGTTGCCCGGCATCCACCTCGATCAACGGCAGGTCCGGTTGCAGCAACACCTTCAAGTCAATc > 2:1201453/1‑150 (MQ=255) tCGACAGTCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGAGCAACACCATCTGCAGTTGCCCGGCATCCACCTCGATCAACGGCa > 2:1112418/1‑109 (MQ=255) tCGACAGTCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGAGCAACACCATCTGCAGTTGCCCGGCATCCACCTCGATCAACGGCa < 1:1112418/109‑1 (MQ=255) gACAGTCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGCTTGAGCAACACCATCTGCAGTTGCCCGGCATCCACCTCGATCAACGGCAGGTCCGGTTGCAGCAACACCTTCAAGTCAATCGAGCTTGGCaa > 1:448604/1‑150 (MQ=255) aGTCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGAGCAACACCATCt > 2:999927/1‑71 (MQ=255) aGTCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGAGCAACACCATCt < 1:999927/71‑1 (MQ=255) tCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGAGCAACACCATCTGCAGTTGCCCGGCATCCACCTCGATCAACGGCa < 1:936847/102‑1 (MQ=255) tCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGAGCAACACCATCTGCAGTTGCCCGGCATCCACCTCGATCAACGGCa > 2:936847/1‑102 (MQ=255) cAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGAGCAACACCATCTGCAGTTGCCCGGCATCCACCTCGATCAACGGCAGGTCCGGTTGCAGCAACACCTTCAAGTCAATCGAGCTTGGCAATGCATg < 1:1091222/150‑1 (MQ=255) gATGCGGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGAGCAACACCATCTGCAGTTGCCCGGCATCCACCTCGATCAACGGCAGGTCCGGTTGCAGCAACACCTTCAAGTCAATCGAGCTTGGCAATGCATGTTCAAg < 1:1201453/150‑1 (MQ=255) aTGCCCGT‑GATGCCATCGACGGGCATTGAACAACAGGTTGAGCAACACCATCTGCAGTTGCCCGGCATCCACCTCGATCAACGGCAGGTCCGGTTGCAGCAACACCTTCaa < 1:809935/111‑1 (MQ=255) aTGCCCGT‑GATGCCATCGACGGGCATTGAACAACAGGTTGAGCAACACCATCTGCAGTTGCCCGGCATCCACCTCGATCAACGGCAGGTCCGGTTGCAGCAACACCTTCaa > 2:809935/1‑111 (MQ=255) ccGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGAGCAACACCATCTGCAGTTGCCCGGCATCCACCTCGATCAACGGCAGGTCCGGTTGCAGCAACACCTTCAAGTCAATCGAGCTTGGCAATGCATGTTCAAGCAGGCCACGCGTa > 1:955178/1‑150 (MQ=255) ggCATC‑ACGGGGATTGAACCACAGGTTGAGCAACACCATCTGCAGTTGCCCGGCATCCACCTCGATCAACGGCAGGTCCGGTTGCAGCAACACCTTCAAGTCAATCGAGCTTGGCAATGCATGTTCAAGCAGGCCACGCGTAGCGTTAAc < 2:448604/150‑1 (MQ=255) | GAAGATGCGCGGCAGTATCTCTGTGGGTATGCCTTCGCCGCTGTCTTCCACCTCGAAGCGCACGAAGCTGCCCTTCAGCCCTTCAACTTCGCCGAACAACTCGACCACATCGACAGTCAGGCTGATGCGGCCATGCCCGTCGATGGCATC‑ACGGGCATTGAACAACAGGTTGAGCAACACCATCTGCAGTTGCCCGGCATCCACCTCGATCAACGGCAGGTCCGGTTGCAGCAACACCTTCAAGTCAATCGAGCTTGGCAATGCATGTTCAAGCAGGCCACGCGTAGCGTTAAC > NC_002947/4360188‑4360481 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |