Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_002947	6,098,315	Δ9 bp	26.8%	coding (822‑830/867 nt)	PP_5350 ←	RpiR family transcriptional regulator

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	6098324 =	53 (1.080)	18 (0.400)	18/228	NT	26.8%	coding (821/867 nt)	PP_5350	RpiR family transcriptional regulator
	?	NC_002947	= 6098314	50 (1.120)					coding (831/867 nt)	PP_5350	RpiR family transcriptional regulator

AACCTGGCGATCGACGTGCATGAAGACACCGAGATCTACACCCCACTGACCTCGCGTATTGCCCACCTGGTGGTGATCGACGTGCTGGCCATGGGCGTTGCCATGGCGCGCGGGCCTAGCCTGGTCAACCACCTGAAGAGCGTGAAACGCAGCTTGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_002947/6098481‑6098324 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgcagcttgcgTCTGTCGCCCAAGTCGATCAAGGCTACCGACGACTGAATTCAACCTGTACTGGCCTGTTCGCGGGCTTGCCCGCTCCCACAGGTACTGTGCACTTCTATAAATCGGTGCAATTCCTGTGTGAACGGGGCAAGCCCGCGAAGAGGC  <  NC_002947/6098314‑6098170                                                                                                                                                                                                                                                                                                                 AACCTGGCGATCGACGTGCATGAAGACACCGAGATCTACACCCCACTGACCTCGCGTATTGCCCACCTGGTGGTGATCGACGTGCTGGCCATGGGCGTTGCCATGGCGCGCGGGCCTAGCCTGGTCAACCACCTGAAGAGCGTGAAACGC                                                                                                                                                           >  2:953823/1‑150             GACGTGCATGAAGACACCGAGATCTACACCCCACTGACCTCGCGTATTGCCCACCTGGTGGTGATCGACGTGCTGGCCATGGGCGTTGCCATGGCGCGCGGGCCTAGCCTGGTCAACCACCTGAAGAGCGTGAAACGCAGCTTGCGTCTG                                                                                                                                               >  1:860897/1‑150                  GCATGAAGACACCGAGATCTACACCCCACTGACCTCGCGTATTGCCCACCTGGTGGTGATCGACGTGCTGGCCATGGGCGTTGCCATGGCGCGCGGGCCTAGCCTGGTCAACCACCTGAAGAGCGTGAAACGCAGCTTGCGTCTGTCGCC                                                                                                                                          >  1:597840/1‑150                              CGAGATCTACACCCCACTGACCTCGCGTATTGCCCACCTGGTGGTGATCGACGTGCTGGCCATGGTCGTTGCCATGGCGCGCGGGCCTAGCCTGGTCAACCACCTGAAGAGCGTGAAACGCAGCTTGCGTCTGTCGCCCAAGTCGATCAA                                                                                                                              >  1:94729/1‑150                               GAGATCTACACCCCACTGACCTCGCGTATTGCCCACCTGGTGGTGATCGACGTGCTGGCCATGGTCGTTGCCATGGCGCGCGGGCCTAGCCTGGTCAACCACCTGAAGAGCGTGAAACGCAGCTTGCGTCTGTCGCCCAAGTCGATCAAG                                                                                                                             <  2:94729/150‑1                                              CTGACCTCGCGTATTGCCCACCTGGTGGTGATCGACGTGCTGGCCATGGGCGTTGCCATGGCGCGCGGGCCTAGCCTGGTCAACCACCTGAAGAGCGTGAAACGCAG                                                                                                                                                         <  1:1126319/107‑1                                              CTGACCTCGCGTATTGCCCACCTGGTGGTGATCGACGTGCTGGCCATGGGCGTTGCCATGGCGCGCGGGCCTAGCCTGGTCAACCACCTGAAGAGCGTGAAACGCAG                                                                                                                                                         >  2:1126319/1‑107                                                   CTCGCGTATTGCCCACCTGGTGGTGATCGACGTGCTGGCCATGGGCGTTGCCATGGCGCGCGGGCCTAGCCTGGTCAACCACCTGAAGAGCGTGAAACGCAGCTTGCGTCTGTCGCCCAAGTCGATCAAGGCTACCGACGACTGAATTCA                                                                                                         >  2:425172/1‑150                                                                         GTGATCGACGTGCTGGCCATGGGCGTTGCCATGGCGCGCGGGCCTAGCCTGGTCAACCACCTGAAGAGCGTGAAACGCAGCTTGCGTCTGTCGCCCAAGTCGATCAAGGCTACCGACGACTGAATTCAACCTGTACTGGCCTGTTCGCGG                                                                                   >  1:553422/1‑150                                                                                   TGCTGGCCATGGGCGTTGCCATGGCGCGCGGGCCTAGCCTGGTCAACCACCTGAAGAGCGTGAAACGCAGCTTGCGTCTGTCGCCCAAGTCGATCAAGGCTACCGACGACTGAATTCAACCTGTACTGGCCTGTTCGCGGGCTTGCCCGC                                                                         <  2:553422/150‑1                                                                                    GCTGGCCATGGGCGTTGCCATGGCGCGCGGGCCTAGCCTGGTCAACCACCTGAAGAGCGTGAAACGCAGCTTGCGTCTGTCGCCCAAGTCGATCAAGGCTACCGACGACTGAATTCAACCTGTACTGGCCTGTTCGCGGGCTTGCCCGCT                                                                        >  1:982287/1‑150                                                                                        GCCATGGGCGTTGCCATGGCGCGCGGGCCTAGCCTGGTCAACCACCTGAAGAGCGTGAAACGCAGCTTGCGTCTGTCGCCCAAGTCGATCAAGGCTACCGACGACTGAATTCAACCTGTACTGGCCTGTTCGCGGGCTTGCCCGCTCCCA                                                                    <  2:860897/150‑1                                                                                           ATGGGCGTTGCCATGGCGCGCGGGCCTAGCCTGGTCAACCACCTGAAGAGCGTGAAACGCAGCTTGCGTCTGTCGCCCAAGTCGATCAAGGCTACCGACGACTGAATTCAACCTGTACTGGCCTGTTCGCGGGCTTGCCCGCTCCCACAG                                                                 >  1:961572/1‑150                                                                                                           CGCGCGGGCCTAGCCTGGTCAACCACCTGAAGAGCGTGAAACGCAGCTTGCGTCTGTCGCCCAAGTCGATCAAGGCTACCGACGACTGAATTCAACCTGTACTGGCCTGTTCGCGGGCTTGCCCGCTCCCACAGGTACTGTGCACTTCTA                                                 <  2:597840/150‑1                                                                                                            GCGCGGGCCTAGCCTGGTCAACCACCTGAAGAGCGTGAAACGCAGCTTGCGTCTGTCGCCCAAGTCGATCAAGGCTACCGACGACTGAATTCAACCTGTACTGGCCTGTTCGCGGGCTTGCCCGCTCCCACAGGTACTGTGCACTTCTAT                                                <  2:961572/150‑1                                                                                                                    CTAGCCTGGTCAACCACCTGAAGAGCGTGAAACGCAGCTTGCGTCTGTCGCCCAAGTCGATCAAGGCTACCGACGACTGAATTCAACCTGTACTGGCCTGTTCGCGGGCTTGCCCGCTCCCACAGGTACTGTGCACTTCTATAAATCGGT                                        <  2:982287/150‑1                                                                                                                     TAGCCTGGTCAACCACCTGAAGAGCGTGAAACGCAGCTTGCGTCTGTCGCCCAAGTCGATCAAGGCTACCGACGACTGAATTCAACCTGTACTGGCCTGTTCGCGGGCTTGCCCGCTCCCACAGGTACTGTGCACTTCTATAAATCGGTG                                       <  1:425172/150‑1                                                                                                                       GCCTGGTCAACCACCTGAAGAGCGTGAAACGCAGCTTGCGTCTGTCGCCCAAGTCGATCAAGGCTACCGACGACTGAATTCAACCTGTACTGGCCTGTTCGCGGGCTTGCCCGCTCCCACAGGTACTGTGCACTTCTATAAATCGGTGCA                                     >  2:903193/1‑150                                                                                                                            GTCAACCACCTGAAGAGCGTGAAACGCAGCTTGCGTCTGTCGCCCAAGTCGATCAAGGCTACCGACGACTGAATTCAACCTGTACTGGCCTGTTCGCGGGCTTGCCCGCTCCCACAGGTACTGTGCACTTCT                                                  >  1:1034232/1‑132                                                                                                                            GTCAACCACCTGAAGAGCGTGAAACGCAGCTTGCGTCTGTCGCCCAAGTCGATCAAGGCTACCGACGACTGAATTCAACCTGTACTGGCCTGTTCGCGGGCTTGCCCGCTCCCACAGGTACTGTGCACTTCT                                                  <  2:1034232/132‑1                                                                                                                               AACCACCTGAAGAGCGTGAAACGCAGCTTGCGTCTGTCGCCCAAGTCGATCAAGGCTACCGACGACTGAATTCAACCTGTACTGGCCTGTTCGCGGGCTTGCCCGCTCCCACAG                                                                 <  1:649099/114‑1                                                                                                                               AACCACCTGAAGAGCGTGAAACGCAGCTTGCGTCTGTCGCCCAAGTCGATCAAGGCTACCGACGACTGAATTCAACCTGTACTGGCCTGTTCGCGGGCTTGCCCGCTCCCACAG                                                                 >  2:649099/1‑114                                                                                                                                                       AGCTTGCGTCTGTCGCCCAAGTCGATCAAGGCTACCGACGACTGAATTCAACCTGTACTGGCCTGTTCGCGGGCTTGCCCGCTCCCACAGGTACTGTGCACTTCTATAAATCGGTGCAATTCCTGTGTGAACGGGGCAAGCCCGCGAAGA     <  1:245891/150‑1                                                                                                                                                          TTGCGTCTGTCGCTCAAGTCGATCAAGGCTACCGACGACTGAATTCAACCTGTACTGGCCTGTTCGCTGGCTTGCCCGCTCCCACAGGTACTGTGCACTTCTATAAATCGGGGCAAT                                   <  2:890572/117‑1                                                                                                                                                          TTGCGTCTGTCGCTCAAGTCGATCAAGGCTACCGACGACTGAATTCAACCTGTACTGGCCTGTTCGCGGGCTTGCCCGCTCCCACAGGTACTGTGCACTTCTATAAATCGGTGCAAT                                   >  1:890572/1‑117                                                                                                                                                          TTGCGTCTGTCGCCCAAGTCGATCAAGGCTACCGACGACTGAATTCAACCTGTACTGGCCTGTTCGCGGGCTTGCCCGCTCCCACAGGTACTGTGCACTTCTATAAATCGGTGCAATTCCTGTGTGAACGGGGCAAGCCCGCGAAGAGGC  >  1:895791/1‑150                                                                                                                                                          TTGCGTCTGTCGCCCAAGTCGATCAAGGCTACCGACAACTCATTTCAACCTGTACTGGCCTGTTCGCGGGCTTGCCCGCTCCCACAGGTACTGTGCACTTCTATAAATCGGTGCAATTCCTGTGTGAACGGGGCAAGCCCGCGAAGAGGC  <  1:654437/150‑1                                                                                                                                                                                                                                                                                                                 AACCTGGCGATCGACGTGCATGAAGACACCGAGATCTACACCCCACTGACCTCGCGTATTGCCCACCTGGTGGTGATCGACGTGCTGGCCATGGGCGTTGCCATGGCGCGCGGGCCTAGCCTGGTCAACCACCTGAAGAGCGTGAAACGCAGCTTGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_002947/6098481‑6098324 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgcagcttgcgTCTGTCGCCCAAGTCGATCAAGGCTACCGACGACTGAATTCAACCTGTACTGGCCTGTTCGCGGGCTTGCCCGCTCCCACAGGTACTGTGCACTTCTATAAATCGGTGCAATTCCTGTGTGAACGGGGCAAGCCCGCGAAGAGGC  <  NC_002947/6098314‑6098170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.