Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 449,734 G→T 10.6% G301V (GGC→GTC)  PP_0370 → acyl‑CoA dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947449,7340GT10.6% 156.0 / 3.2 53G301V (GGC→GTC) PP_0370acyl‑CoA dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base G (20/27);  new base T (3/3);  total (23/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGGCTCGATCGAGCACAAAATGGGCATCCAGGCTTCGGCTACCTGTGTGATGAACTTCGACGAGGCTGTCGGCTACATCGTCGGTGAGCCGAACAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCGCCTGCA‑AAGCCGTGCCCCGGCCGGCCCACAAGCCAAGGACAAGGCTGCCGACCCGATCATCGTCCACCCGGACGTGCGTCGCATGC  >  NC_002947/449587‑449883
                                                                                                                                                   |                                                                                                                                                      
gCGGCTCGATCGAGCACAAAATGGGCATCCAGGCTTCGGCTACCTGTGTGATGAACTTCGACGAGGCTGTCGGCTACATCGTCGGTGAGCCGAACAGAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCa                                                                                                                                                      <  1:153270/150‑1 (MQ=255)
 cGGCTCGATCGAGCACAAAATGGGCATCCAGGCTTCGGCTACCTGTGTGATGAACTTCGACGAGGCTGTCGGCTACATCGTCGGTGAGCCGAACAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCAt                                                                                                                                                     <  2:36519/150‑1 (MQ=255)
             gCACAAAATGGGCATCCAGGCTTCGGCTACCTGTGTGATGAACTTCGACGAGGCTGTCGGCTACATCGTCGGTGAGCCGAACAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGc                                                                                                                                         <  2:295993/150‑1 (MQ=255)
                 aaaaTGGGCATCCAGGCTTCGGCTACCTGTGTGATGAACTTCGACGAGGCTGTCGGCTACATCGTCGGTGAGCCGAACAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCAtt                                                                                                                                                    <  2:350569/135‑1 (MQ=255)
                 aaaaTGGGCATCCAGGCTTCGGCTACCTGTGTGATGAACTTCGACGAGGCTGTCGGCTACATCGTCGGTGAGCCGAACAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCAtt                                                                                                                                                    >  1:350569/1‑135 (MQ=255)
                 aaaaTGGGCATCCAGGCTTCGGCTACCTGTGTGATGAACTTCGACGAGGCTGTCGGCTACATCGTCGGTGAGCCGAACAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCg                                                                                                                                     >  2:148734/1‑150 (MQ=255)
                   aaTGGGCATCCAGGCTTCGGCTACCTGTGTGATGAACTTCGACGAGGCTGTCGGCTACATCGTCGGTGAGCCGAACAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGc                                                                                                                                   <  2:749001/150‑1 (MQ=255)
                    aTGGGCATCCAGGCTTCGGCTACCTGTGTGATGAACTTCGACGAGGCTGTCGGCTACATCGTCGGTGAGCCGAACAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGcc                                                                                                                                  <  1:1179089/150‑1 (MQ=255)
                            ccAGGCTTCGGCTACCTGTGTGATGAACTTCGACGAGGCTGTCGGCTACATCGTCGGTGAGCCGAACAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTc                                                                                                                          <  1:17821/150‑1 (MQ=255)
                              aGGCTTCGGCTACCTGTGTGATGAACTTCGACGAGGCTGTCGGCTACATCGTCGGTGAGCCGAACAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCGAGGCCTGGCGTCGGCCGAGCGCTCCt                                                                                                                        <  1:1094135/150‑1 (MQ=255)
                                gCTTCGGCTACCTGTGTGATGAACTTCGACGAGGCTGTCGGCTACATCGTCGGTGAGCCGAACAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTAt                                                                                                                      <  2:679236/150‑1 (MQ=255)
                                  ttCGGCTACCTGTGTGATGAACTTCGACGAGGCTGTCGGCTACATCGTCGGTGAGCCGAACAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCa                                                                                                                    >  2:711655/1‑150 (MQ=255)
                                    cGGCTACCTGTGTGATGAACTTCGACGAGGCTGTCGGCTACATCGTCGGTGAGCCGAACAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGa                                                                                                                  <  1:967167/150‑1 (MQ=255)
                                      gCTACCTGTGTGATGAACTTCGACGAGGCTGTCGGCTACATCGTCGGTGAGCCGAACAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAAc                                                                                                                >  1:652823/1‑150 (MQ=255)
                                           cTGTGTGATGAACTTCGACGAGGCTGTCGGCTACATCGTCGGTGAGCCGAACAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGTCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGt                                                                                                           >  2:430799/1‑150 (MQ=255)
                                            tgtgtgATGAACTTCGACGAGGCTGTCGGCTACATCGTCGGTGAGCCGAACAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTg                                                                                                          <  1:1010630/150‑1 (MQ=255)
                                                 gatgaACTTCGACGAGGCTGTCGGCTACATCGTCGGTGAGCCGAACAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTAAACACTATCCTTGTAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTa                                                                                                     >  2:998431/1‑150 (MQ=255)
                                                       cTTCGACGAGGCTGTCGGCTACATCGTCGGTGAGCCGAACAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCcg                                                                                               >  1:580937/1‑150 (MQ=255)
                                                        ttCGACGAGGCTGTCGGCTACATCGTCGGTGAGCCGAACAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCcgc                                                                                              >  2:351521/1‑150 (MQ=255)
                                                           gacgaGGCTGTCGGCTACATCGTCGGTGAGCCGAACAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGAt                                                                                           >  2:947808/1‑150 (MQ=255)
                                                                  cTGTCGGCTACATCGTCGGTGAGCCGAACAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCGCCt                                                                                      >  1:345573/1‑148 (MQ=255)
                                                                  cTGTCGGCTACATCGTCGGTGAGCCGAACAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCGCCt                                                                                      <  2:345573/148‑1 (MQ=255)
                                                                   tGTCGGCTACATCGTCGGTCAGCCGAACAAAGGCATTGCGGCCATGTTGACCATGATGAACTACGAGCGCCTGGGCGTAGGCATTCACGGAGTCGCATATGCCGAGCGCGCCTATCAGAACCCCGGGGAGTACGCCCGCGATCGCCTGCa                                                                                   >  2:920233/1‑150 (MQ=255)
                                                                    gTCGGCTACATCGTCGGTGAGCCGAACAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCGCCTGCa‑a                                                                                 >  2:64720/1‑150 (MQ=255)
                                                                          tACATCGTCGGTGAGCCGAACAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGTCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCGCCTGCA‑AAGCCGt                                                                           <  1:430799/150‑1 (MQ=255)
                                                                          tACATCGTCGGTGAGCCGAACAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCGCCTGCA‑AAGCCGt                                                                           <  1:64720/150‑1 (MQ=255)
                                                                                gtcgGTGAGCCGAACAAAGGCTTGGCGGCCATGTTCACCATGATNAACNACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCGCCTGCA‑AAGCCGTGCCccg                                                                     >  1:659348/1‑150 (MQ=255)
                                                                                        gCCGAACAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCGCCTGCA‑AAGCCGTGCCCCGGCCGGccc                                                             >  1:211804/1‑150 (MQ=255)
                                                                                            aaCAAAGGCTTGGCGGCCATGTTCACCATGATGATCTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCCCCTTCA‑AAGCCGTGCCCCGGCCGGCCCACaa                                                         <  1:836414/150‑1 (MQ=255)
                                                                                            aaCAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATC‑AATACATAAGCCGTGCCCCGGCCGGCCCACaa                                                         <  1:998431/150‑1 (MQ=255)
                                                                                             aCAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCGCCTGCA‑AAGCCGTGCCccggccgg                                                                >  1:270695/1‑142 (MQ=255)
                                                                                             aCAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCGCCTGCA‑AAGCCGTGCCccggccgg                                                                <  2:270695/142‑1 (MQ=255)
                                                                                             aCAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGCCGTCGTCATTCAAGGCCGGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCGCCTGCA‑AAGCCGTGCCCCGGCCGGCCCACAAg                                                        <  1:1050698/150‑1 (MQ=255)
                                                                                               aaaGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCcgc                                                                                              >  1:673810/1‑111 (MQ=255)
                                                                                               aaaGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCcgc                                                                                              <  2:673810/111‑1 (MQ=255)
                                                                                                 aGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCGCCTGCA‑AAGCCGTGCCCCGGCCGGCCCACAAGCCaa                                                    <  1:148734/150‑1 (MQ=255)
                                                                                                   gCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCt                                                                                                                                            <  2:90291/61‑1 (MQ=255)
                                                                                                   gCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCt                                                                                                                                            >  1:90291/1‑61 (MQ=255)
                                                                                                   gCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCGCCTGCA‑AAGCCGTGCCCCGGCCGGCCCACAAGCCAAgg                                                  >  2:1136207/1‑150 (MQ=255)
                                                                                                    cTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCGCCTGCA‑AAGCCGTGCCCCGGCCGGCCCACAAGCCAACGa                                                 <  1:1282346/150‑1 (MQ=255)
                                                                                                       ggcggcCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCGCCTGCA‑AAGCCGTGCCCCGGCCGGCCCACTAGCCAAGGACaa                                              <  2:211804/150‑1 (MQ=255)
                                                                                                            ccATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCGCCTGCA‑AAGCCGTGCCCCGGCCGGCCCACAAGCCAAGGACAAGGCt                                          <  1:710874/149‑1 (MQ=255)
                                                                                                            ccATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCGCCTGCA‑AAGCCGTGCCCCGGCCGGCCCACAAGCCAAGGACAAGGCt                                          >  2:710874/1‑149 (MQ=255)
                                                                                                             cATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCGCCTGCA‑AAGCCGTGCCCCGGCCGGCCCACAAGCCAAGGACAAGGCTGc                                        <  1:711655/150‑1 (MQ=255)
                                                                                                             cATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCGGTCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCGCCTGCA‑AAGCCGTGCCCCGGCCGGCCCACAAGCCAAGGACAAGGCTGc                                        <  1:920233/150‑1 (MQ=255)
                                                                                                                   cACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCGCCTGCA‑AAGCCGTGCCCCGGCCGGCCCACAAGCCAAGGACAAGGCTGCCGAccc                                  <  2:652823/150‑1 (MQ=255)
                                                                                                                        tgatgaACTACGAGCGCCTGGGCGTCGTCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCGCCTGCA‑AAGCCGTGCCccggc                                                                   >  2:1251169/1‑112 (MQ=255)
                                                                                                                        tgatgaACTACGAGCGCCTGGGCGTCGTCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCGCCTGCA‑AAGCCGTGCCccggc                                                                   <  1:1251169/112‑1 (MQ=255)
                                                                                                                        tgatgaACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCGCCTGCA‑AAGCCGTGCCCCGGCCGGCCCACAAGCCAAGGACAAGGCTGCCGACCCGatca                             >  2:171896/1‑150 (MQ=255)
                                                                                                                          atgaACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCGCCTGCA‑AAGCCGTGCCCCGGCCGGCCCACAAGCCAAGGACAAGGCTGCCGACCCGatcatc                           <  1:351521/150‑1 (MQ=255)
                                                                                                                                   gAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCGCCTGCA‑AAGCCGTGCCCCGGCCGGCCCACAAGCCAAGGACAAGGCTGCCGACCCGATCATCGTCCACCCg                  >  2:99812/1‑150 (MQ=255)
                                                                                                                                          tGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCGCCTGCA‑AAGCCGTGCCCCGGCCGGCCCACAAGCCAAAGACAAGGCTGCCGACCCGATCATCGTCCACCCCGCCGTGc           >  1:68436/1‑150 (MQ=255)
                                                                                                                                                 cGGCATTCAAGGCCTGGCGCCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCGCCTGCA‑AAGCCGTGCCCCGGCCGGCCCACAAGCCAAGGACAAGGCTGCCGACCCGATCATCGTCCACCCGGACGTGCGTCGCAt    <  1:462326/150‑1 (MQ=255)
                                                                                                                                                   gCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCGCCTGCA‑AAGCCGTGCCCCGGCCGGCCCACAAGCCAAGGACAAGGCTGCCGACCCGATCATCGTCCACCCGGACGTGCGTCGCATGc  >  2:273515/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
GCGGCTCGATCGAGCACAAAATGGGCATCCAGGCTTCGGCTACCTGTGTGATGAACTTCGACGAGGCTGTCGGCTACATCGTCGGTGAGCCGAACAAAGGCTTGGCGGCCATGTTCACCATGATGAACTACGAGCGCCTGGGCGTCGGCATTCAAGGCCTGGCGTCGGCCGAGCGCTCCTATCAGAACGCCGTGGAGTACGCCCGCGATCGCCTGCA‑AAGCCGTGCCCCGGCCGGCCCACAAGCCAAGGACAAGGCTGCCGACCCGATCATCGTCCACCCGGACGTGCGTCGCATGC  >  NC_002947/449587‑449883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑