| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 461,033 | C→G | 7.7% | V479L (GTG→CTG) | pqqF ← | coenzyme PQQ synthesis protein F |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 461,033 | 0 | C | G | 7.7% | 200.9 / 8.1 | 65 | V479L (GTG→CTG) | pqqF | coenzyme PQQ synthesis protein F |
| Reads supporting (aligned to +/- strand): ref base C (28/32); new base G (2/3); total (30/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.91e-01 | |||||||||||
GGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGGCGCATAGGTGAAGGTA > NC_002947/460886‑461182 | ggggCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCActg > 1:185170/1‑150 (MQ=255) aGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCATGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCActgctcct < 1:1282287/150‑1 (MQ=255) tACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGGGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCAct > 1:53515/1‑140 (MQ=255) tACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGGGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCAct < 2:53515/140‑1 (MQ=255) tACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCa < 2:181214/150‑1 (MQ=255) ccccGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAgg > 2:309499/1‑150 (MQ=255) cccGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGc > 1:698217/1‑150 (MQ=255) cccGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGc > 1:370222/1‑150 (MQ=255) cccGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGc > 1:163292/1‑150 (MQ=255) aCCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGgcg < 2:1242241/150‑1 (MQ=255) ccGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGgcgc > 1:989908/1‑150 (MQ=255) cgcgTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGTCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCa < 1:605380/150‑1 (MQ=255) cgTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGt < 2:1074543/150‑1 (MQ=255) cgTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGt < 1:484335/150‑1 (MQ=255) gTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCActgctg < 2:844762/131‑1 (MQ=255) gTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCActgctg > 1:844762/1‑131 (MQ=255) gCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACt > 1:219949/1‑150 (MQ=255) gCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCAGCAGCAgtgctgcggg > 2:4479/1‑127 (MQ=255) gCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCAGCAGCAGTGCTGCggg < 1:4479/130‑1 (MQ=255) ttGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCa < 2:912071/150‑1 (MQ=255) ttGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTAt > 1:207482/1‑150 (MQ=255) ttGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTAt > 2:448359/1‑150 (MQ=255) gCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTccc < 1:1282003/150‑1 (MQ=255) gCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTccc > 1:1194274/1‑150 (MQ=255) ggggATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTa < 2:176772/150‑1 (MQ=255) ggATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGAATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACt < 1:282294/150‑1 (MQ=255) aTCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGc > 2:677409/1‑150 (MQ=255) gCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGc > 1:1220648/1‑150 (MQ=255) cGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCAc < 1:1081969/150‑1 (MQ=255) gggggCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACg < 2:370222/150‑1 (MQ=255) ggCAGCGCCGTGCCGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCCTCAAGGCCAAGGCTTGTTCCACACCACGACCCACTGCTGCTGGCAGGCCGGCGCAGTGCAATTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAg < 2:43635/150‑1 (MQ=255) gcCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCgg > 1:1083691/1‑150 (MQ=255) ggCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGt < 1:382755/150‑1 (MQ=255) ggCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGGTGCACGGAGGCGCGGTCACaatgt > 1:325208/1‑147 (MQ=255) cagccagcAACTGGCAGGTTGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTc > 2:245474/1‑150 (MQ=255) cagccagcAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTc < 2:1194274/150‑1 (MQ=255) gccagcAACTGGCAGGTTGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCt < 1:245474/150‑1 (MQ=255) ccagcAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTg < 2:475224/150‑1 (MQ=255) cagcAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGc > 1:596087/1‑150 (MQ=255) agcAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCa < 1:309499/150‑1 (MQ=255) gcAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAg < 2:163292/150‑1 (MQ=255) aaCTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTg > 2:756868/1‑150 (MQ=255) aaCTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTg < 1:136180/150‑1 (MQ=255) aaCTGGCAGGTGGCTTGAGCATCAAGGCCAACGCTTATTCCACTGCCCTCAGCACTGCTGCTGGCAGGCCGGTGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACATGCCTGCAGTg > 1:731558/1‑150 (MQ=255) aaCTGGCAGGTGGCTTGAGCAGCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGTCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTg > 1:103760/1‑150 (MQ=255) gCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGcc > 2:1001519/1‑150 (MQ=255) aGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGtt < 1:105246/134‑1 (MQ=255) aGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGtt > 2:105246/1‑134 (MQ=255) aGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAg < 1:219779/150‑1 (MQ=255) aGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAg < 1:312518/150‑1 (MQ=255) tGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGc < 1:448359/150‑1 (MQ=255) ggCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGcc < 2:1083691/150‑1 (MQ=255) ttGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCa > 2:184313/1‑106 (MQ=255) ttGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCa < 1:184313/106‑1 (MQ=255) gAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTc < 2:185170/150‑1 (MQ=255) gCATCCAGGCCAGGGCTTGTTCCACTGCTCGCAGCACTGCTGCTGGGAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGCCACTGCTGTACTGCAGTTGCCCGGAGGCGCGGTCACAATGTTCCTGCAGTGGCGCCAGGGCCTGTTCCa < 2:207482/150‑1 (MQ=255) gCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAATGTTCCTGCAGTGGCGCCAGGGCCTGTTCCa < 2:325208/150‑1 (MQ=255) gCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCa < 2:219949/150‑1 (MQ=255) aggccaaggcTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCacac > 2:331994/1‑150 (MQ=255) aggccaaggcTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCacac > 2:881511/1‑150 (MQ=255) gTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCt > 2:950947/1‑150 (MQ=255) ttCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTg > 2:1254308/1‑150 (MQ=255) cACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGgc > 2:318483/1‑112 (MQ=255) cACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGgc < 1:318483/112‑1 (MQ=255) cACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGc > 1:65938/1‑136 (MQ=255) cACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGc < 2:65938/136‑1 (MQ=255) cTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGGCGCa < 2:989908/150‑1 (MQ=255) ccagcagcaGTGCTGCGGGCAGTCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCAGTGCTGTACTGCAGTTGCa < 1:214483/72‑1 (MQ=37) ccagcagcaGTGCTGCGGGCAGTCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCAGTGCTGTACTGCAGTTGCa > 2:214483/4‑75 (MQ=37) ccagcagcaGTGCTGCGGGCAGTCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCAATGCTGTACTGCAGTTGCa < 1:214530/72‑1 (MQ=37) ccagcagcaGTGCTGCGGGCAGTCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCAATGCTGTACTGCAGTTGCa > 2:214530/4‑75 (MQ=37) cGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGGCGCATAGGt > 1:270866/1‑150 (MQ=255) ctgctgctgGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGGCGCATAGGTGAAGGTa < 1:756868/150‑1 (MQ=255) | GGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCTGGCAGGCCGGCGCAGTGCAACTGCCAGTATTCCCCGGCACTGCTGTACTGCAGTTGCACGGAGGCGCGGTCACAACGTTCCTGCAGTGGCGCCAGGGCCTGTTCCAGCACACGCTGTAACGGCTGGCGCATAGGTGAAGGTA > NC_002947/460886‑461182 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |