Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 471,070 G→A 9.4% intergenic (‑111/+17) PP_0386 ← / ← rpoD sensory box protein/RNA polymerase sigma 70 factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947471,0700GA 9.4% 133.8 / 5.4 53intergenic (‑111/+17)PP_0386/rpoDsensory box protein/RNA polymerase sigma 70 factor
Reads supporting (aligned to +/- strand):  ref base G (24/24);  new base A (2/3);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.27e-01

GATCAGCAACAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGG  >  NC_002947/470927‑471219
                                                                                                                                               |                                                                                                                                                     
gATCAGCAACAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCgg                                                                                                                                                 >  1:1075070/1‑150 (MQ=255)
  tCAGCAACAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCgggg                                                                                                                                               <  1:524056/150‑1 (MQ=255)
  tCAGCAACAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCCAGGCCgggg                                                                                                                                               >  2:1086429/1‑150 (MQ=255)
       aaCAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCGCGGGCCACTAAAGAAAAACCCCGGCCCAGGCCGGGGttttt                                                                                                                                          <  2:208685/150‑2 (MQ=255)
       aaCAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTg                                                                                                                                          >  1:1100553/1‑150 (MQ=255)
        aCAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCCAGGCCGGGGtttttc                                                                                                                                         <  1:1086429/150‑3 (MQ=255)
         cAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGtc                                                                                                                                        >  2:271006/1‑150 (MQ=255)
                 ccGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACtcg                                                                                                                                <  2:187068/150‑1 (MQ=255)
                  cGGCTTCAAGGACATGGCAGCATCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACtcgt                                                                                                                               >  2:84795/1‑150 (MQ=255)
                   ggCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGtttt                                                                                                                                           >  2:1070138/1‑137 (MQ=255)
                   ggCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGtttt                                                                                                                                           <  1:1070138/137‑1 (MQ=255)
                     cTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGa                                                                                                                            <  1:76049/150‑1 (MQ=255)
                      ttCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGt                                                                                                                                         >  1:593270/1‑136 (MQ=255)
                      ttCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGt                                                                                                                                         <  2:593270/136‑1 (MQ=255)
                          aGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGaggaag                                                                                                                       >  2:708735/1‑150 (MQ=255)
                                 ggCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCa                                                                                                                <  2:1075070/150‑1 (MQ=255)
                                  gCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAg                                                                                                               <  2:261417/150‑1 (MQ=255)
                                      ccTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGc                                                                                                           >  2:185208/1‑150 (MQ=255)
                                              tCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTg                                                                                                                                          <  2:1234172/111‑1 (MQ=255)
                                              tCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTg                                                                                                                                          >  1:1234172/1‑111 (MQ=255)
                                              tCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCg                                                                                                   >  2:319901/1‑150 (MQ=255)
                                                aGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCCGGTGCTCGCTGCGAg                                                                                                 >  1:894333/1‑150 (MQ=255)
                                                      tCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGt                                                                                           <  1:25486/150‑1 (MQ=255)
                                                          aaGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGAAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGgcg                                                                                       >  2:432756/1‑150 (MQ=255)
                                                                 gtgtAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTg                                                                                >  1:237590/1‑150 (MQ=255)
                                                                  tgtAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACtcg                                                                                                                                <  1:116944/101‑1 (MQ=255)
                                                                  tgtAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACtcg                                                                                                                                >  2:116944/1‑101 (MQ=255)
                                                                  tgtAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTgc                                                                               >  1:154634/1‑150 (MQ=255)
                                                                       aCGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAAc                                                                          >  1:947855/1‑150 (MQ=255)
                                                                       aCGGGCATTGCCGCAGGTGTGCAACGGAAGACGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAAc                                                                          <  1:637849/150‑1 (MQ=255)
                                                                         gggCATTGCCGCAGGTGTGCAACGGAAGGCGGGCGTTATGTCACGGGCCACTAAAGAAAAACACCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGc                                                                        >  2:130923/1‑150 (MQ=255)
                                                                                  cGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCcttcgcttc                                                               <  1:126644/150‑1 (MQ=255)
                                                                                    cAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGa                                                             <  1:432756/150‑1 (MQ=255)
                                                                                      ggTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATc                                                           <  1:319901/150‑1 (MQ=255)
                                                                                       gtgtGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTg                                                                                                                                                       <  2:521962/57‑1 (MQ=255)
                                                                                       gtgtGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTg                                                                                                                                                       >  1:521962/1‑57 (MQ=255)
                                                                                       gtgtGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCt                                                          <  1:271006/150‑1 (MQ=255)
                                                                                       gtgcGCAACGGAAGGTGGGCGTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCACGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCt                                                          <  2:673525/150‑1 (MQ=255)
                                                                                          tgCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGAc                                                       <  2:485991/150‑1 (MQ=255)
                                                                                              aCGGAAGGCCGTCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGAt                                                   <  2:894333/150‑1 (MQ=255)
                                                                                                ggAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATcc                                                 <  2:1100553/150‑1 (MQ=255)
                                                                                                      cGGGCTTTCTGTCACTGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCAc                                           <  1:379858/150‑1 (MQ=255)
                                                                                                           tttATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTAGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTTGTTTGGCGCAGATTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGtt                                      >  1:13276/1‑150 (MQ=255)
                                                                                                            ttATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTa                                     <  1:708735/150‑1 (MQ=255)
                                                                                                                gTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTc                                 >  2:954264/1‑150 (MQ=255)
                                                                                                                     gggCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAAGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCACAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCGCACGCGTTACGTCAAACt                            >  2:500213/1‑150 (MQ=255)
                                                                                                                         cACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCtt                        >  2:1139066/1‑150 (MQ=255)
                                                                                                                                  aaaaCCCCGGCCCAGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATGTGACGGATCCGCTCa                                            >  2:925099/1‑121 (MQ=255)
                                                                                                                                  aaaaCCCCGGCCCAGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCa                                            <  1:925099/121‑1 (MQ=255)
                                                                                                                                      ccccGGCCTGGGCCGGGGTTTTGTCATGACTCGTCGAGGACGGAGCGCAGGTGCTCGCCGCTAGGGTGATGCCGCAGCTTGCGCAACGCCTGCGCTTCGATCTGACGGATCCGCGTACGTGTTACGTCAAACGGCTGGCCAACCtcgtcg           >  2:218161/1‑150 (MQ=255)
                                                                                                                                      ccccGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCg           >  2:198474/1‑150 (MQ=255)
                                                                                                                                       cccGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCtt                        <  2:576696/136‑1 (MQ=255)
                                                                                                                                       cccGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCtt                        >  1:576696/1‑136 (MQ=255)
                                                                                                                                         cGGCCTGGGCCGGGGTTTTGTCATCACTCGCCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCGTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAgg        <  1:84795/150‑1 (MQ=255)
                                                                                                                                           gCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGgt      <  1:954264/150‑1 (MQ=255)
                                                                                                                                               gggCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTgg  <  1:198474/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
GATCAGCAACAGAATGGCCGGCTTCAAGGACATGGCAGCCTCGCACTCAGGTGGTCTGAAGTCGAGTGTAGACGGGCATTGCCGCAGGTGTGCAACGGAAGGCGGGCTTTATGTCACGGGCCACTAAAGAAAAACCCCGGCCTGGGCCGGGGTTTTGTCATCACTCGTCGAGGAAGGAGCGCAGGTGCTCGCTGCGAGTCGGGTGGCGCAGCTTGCGCAACGCCTTCGCTTCGATCTGACGGATCCGCTCACGCGTTACGTCAAACTGCTTGCCCACCTCTTCGAGGGTGTGG  >  NC_002947/470927‑471219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑