Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	503,834	A→T	10.9%	intergenic (+34/‑117)	PP_0414 → / → rpe	polyamine ABC transporter permease/ribulose‑5‑phosphate 3‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	503,834	0	A	T	10.9%	151.3 / 9.0	54	intergenic (+34/‑117)	PP_0414/rpe	polyamine ABC transporter permease/ribulose‑5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base A (24/24);  new base T (4/2);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

CCGACCATTGCCGCGGCGGCGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGCCCCCTCC  >  NC_002947/503687‑503977                                                                                                                                                    |                                                                                                                                                 ccGATCATTGCCGCGGTGGCGACCTTGGTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTaaa                                                                                                                                                <  2:489310/150‑1 (MQ=255)        ttGCCGCGGCGGCGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑Gaa                                                                                                                                        <  1:197077/150‑1 (MQ=255)           ccGCGGCGGCGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAagag                                                                                                                                     <  1:70488/150‑1 (MQ=255)                                 tGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGTCGGCACAGGTTGCGTTGGTc                                                                                                               <  2:454255/150‑1 (MQ=255)                                 tGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTc                                                                                                               <  1:1233977/150‑1 (MQ=255)                                 tGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTACTGGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTc                                                                                                               <  1:1030708/150‑1 (MQ=255)                                   cGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAATGCCG‑TAAGAGGCCGGCACAGGTTGCGTTGGTCGa                                                                                                             <  1:887250/150‑1 (MQ=255)                                    gTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGtt                                                                                                                   <  2:371042/143‑1 (MQ=255)                                    gTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGtt                                                                                                                   >  1:371042/1‑143 (MQ=255)                                           gtgtTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAAtt                                                                                                     <  2:54617/150‑1 (MQ=255)                                                gttgCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATAc                                                                                                <  2:953157/150‑1 (MQ=255)                                                 tgtctgctgACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATAcc                                                                                               >  2:320691/4‑150 (MQ=255)                                                    ctgctgACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGCCTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAAt                                                                                            <  2:793069/150‑1 (MQ=255)                                                         gACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGGGCAGGTTGCGTTGGTCGACACCAATTGATACCAATTAGcc                                                                                       >  1:1135582/1‑150 (MQ=255)                                                               ggAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATc                                                                                 >  2:1124454/1‑150 (MQ=255)                                                                          gTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGa                                                                      >  2:569722/1‑150 (MQ=255)                                                                          gTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGa                                                                      <  1:320691/150‑1 (MQ=255)                                                                               catctcGAGAATCTTAGGACTCAACAGCCGGCTTCATGATGTCGTGCCTGTACCGGCCTCTTCGCCGCTAAAGCCT‑TAAGAGGCCGGCACAGGTTGCGTTGGTCTACACCACTTACTGCCAATCAGCCTTCATCGCTTTCCCTGAGTTAc                                                                 <  1:1066639/145‑1 (MQ=255)                                                                                gTAGCGAGAAGCTGAGGACTCAACAGACGGCTTGATGATGGCTTGCCTTTACCGGCCACTTCGCGGCTAAAGCCG‑GAAGAGGCCGGGACAGGTTGCGTTGATCGACACCAACTGCAACAAATTAACGCTCATCGCTTACCCTGAGATACa                                                                >  1:301734/1‑150 (MQ=255)                                                                                     gagaAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGaca                                                                                                           >  2:1209363/1‑102 (MQ=255)                                                                                     gagaAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGaca                                                                                                           <  1:1209363/102‑1 (MQ=255)                                                                                     gagaAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATAGCAATGTGCGTTAATCGCTTTCCCTGAGTTACaagatt                                                           >  2:1066639/1‑146 (MQ=255)                                                                                     gagaAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCaa                                                                                             >  2:217654/1‑116 (MQ=255)                                                                                     gagaAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCaa                                                                                             <  1:217654/116‑1 (MQ=255)                                                                                     gagaAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATgcg                                                           >  2:328790/1‑150 (MQ=255)                                                                                      agaAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATgcgc                                                          <  1:328790/150‑1 (MQ=255)                                                                                      agaAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATgcgc                                                          <  2:181935/150‑1 (MQ=255)                                                                                                  cTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAAt                                              <  1:569722/150‑1 (MQ=255)                                                                                                   tCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTg                                                                                                                  <  2:68229/81‑1 (MQ=255)                                                                                                   tCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAAtt                                             >  1:951818/1‑150 (MQ=255)                                                                                                   tCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCAAAGGTTGCGTTg                                                                                                                  >  1:68229/1‑81 (MQ=255)                                                                                                    cAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAAttt                                            <  2:691225/150‑1 (MQ=255)                                                                                                     gacggtgggCTTGATGATGGCGTGCCTGTACCGGCCTCTTCGCGGCTAAAGCAG‑CGAGAGGTCGGTACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGTTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTg                                           <  2:301734/143‑1 (MQ=255)                                                                                                           cGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACa                                     >  2:1088383/1‑150 (MQ=255)                                                                                                             gCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCg                                                                                <  1:1198374/105‑1 (MQ=255)                                                                                                             gCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCg                                                                                >  2:1198374/1‑105 (MQ=255)                                                                                                                    tatgGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCa                            >  1:936098/2‑150 (MQ=255)                                                                                                                      aacgcaaccTGTGCAGGCCTCTTC‑CGGCTTTAGCCGCGAAGAGGCCGGTACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATg                          >  2:212274/8‑150 (MQ=25)                                                                                                                        gCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATAGCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACa                                     <  2:815735/137‑1 (MQ=255)                                                                                                                        gCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACa                                     >  1:815735/1‑137 (MQ=255)                                                                                                                        cgcaaccTGTGCCGGCCTCTTC‑CGGCTTTAGCCGCGAAGAGGCCGGTACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATACa                        >  2:80891/6‑150 (MQ=25)                                                                                                                            gCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGt                                                  >  1:474111/1‑120 (MQ=255)                                                                                                                            gCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGt                                                  <  2:474111/120‑1 (MQ=255)                                                                                                                             ccTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTTCCAACAGTTTCGCCATGCAGCCCt                   >  2:133485/1‑150 (MQ=255)                                                                                                                             ccTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCt                   >  1:871274/1‑150 (MQ=255)                                                                                                                              cTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTa                  >  1:502633/1‑150 (MQ=255)                                                                                                                               tGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTAc                 >  1:935705/1‑150 (MQ=255)                                                                                                                                 tACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGc               >  1:749665/1‑150 (MQ=255)                                                                                                                                     ggCCTCTTC‑CGGCTTTAGCCGCGAAGAGGCCGGTACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTAtt           <  1:212274/150‑1 (MQ=38)                                                                                                                                      gCCTCTTC‑CGGCTTTAGCCGCGAAGAGGCCGGTACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAAt                                                              <  2:730177/98‑1 (MQ=25)                                                                                                                                      gCCTCTTC‑CGGCTTTAGCCGCGAAGAGGCCGGTACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAAt                                                              >  1:730177/1‑98 (MQ=25)                                                                                                                                      gCCTCTTC‑CGGCTTTAGCCGCGAAGAGGCCGGTACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTg          >  1:501310/1‑150 (MQ=38)                                                                                                                                           ttCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGACTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGccccc     >  1:341015/1‑150 (MQ=255)                                                                                                                                            tCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGCCCCCt    <  2:749665/150‑1 (MQ=255)                                                                                                                                              gcgGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGCCCCCTcc  >  1:475183/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                 CCGACCATTGCCGCGGCGGCGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGCCCCCTCC  >  NC_002947/503687‑503977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑