Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 537,575 G→T 7.1% intergenic (+102/‑51) PP_mr07 → / → rpoB P26 ncRNA/DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947537,5750GT 7.1% 207.2 / 2.4 65intergenic (+102/‑51)PP_mr07/rpoBP26 ncRNA/DNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (35/25);  new base T (3/2);  total (38/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTGGCCATCCAGCTG  >  NC_002947/537428‑537721
                                                                                                                                                   |                                                                                                                                                  
tGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAACCGTAGGGGTTTGCACGATTTTCTGgctgct                                                                                                                                                  <  1:998216/150‑1 (MQ=255)
 gAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTc                                                                                                                                                 >  1:104053/1‑150 (MQ=255)
     gTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCccgc                                                                                                                                             >  2:1039992/1‑150 (MQ=255)
               aGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTTCTCCCGGCGGGagcagc                                                                                                                                   <  2:171390/150‑1 (MQ=255)
               aGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGc                                                                                                                                   <  1:1143075/150‑1 (MQ=255)
               aGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGc                                                                                                                                   <  2:911289/150‑1 (MQ=255)
                  cTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTTTGGCTTCTCCCGGCGGGAGCAGCCag                                                                                                                                >  1:256425/1‑149 (MQ=255)
                   tGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCaaa                                                                                                                               <  1:1099805/150‑1 (MQ=255)
                          tggtggCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGGCGGTCAGGCCGGTCCCGCTAACGCGACCCGAGATACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAg                                                                                                                        <  1:862886/150‑1 (MQ=255)
                               gCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACCCTTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGAc                                                                                                                   >  2:169696/1‑150 (MQ=255)
                                cTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTTCTCCCGGCGGGAGCAGcc                                                                                                                                  <  2:713993/134‑1 (MQ=255)
                                cTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTTCTCCCGGCGGGAGCAGcc                                                                                                                                  >  1:713993/1‑134 (MQ=255)
                                 ttATGCCACCGGCCTTTTTCCGTTATTGGTGGCTGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCACCGGGAGAAGCCAAACAAGCAGGTGAcga                                                                                                                 >  1:162894/1‑148 (MQ=255)
                                 ttATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCaa                                                                                                                                <  2:1217476/135‑1 (MQ=255)
                                 ttATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCaa                                                                                                                                >  1:1217476/1‑135 (MQ=255)
                                  tATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCaa                                                                                                                >  1:312723/1‑150 (MQ=255)
                                       cACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTgg                                                                                                           >  2:1022420/1‑150 (MQ=255)
                                       cACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTgg                                                                                                           >  1:751417/1‑150 (MQ=255)
                                        aCCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTggg                                                                                                          >  1:219213/1‑150 (MQ=255)
                                        aCCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTggg                                                                                                          >  1:859501/1‑150 (MQ=255)
                                        aCCGGCCAGTTTCCGGGATGGGTGGCCGGTCAGGCCCGGCCCGATAACGAGCTGCAAGACACCCAAGCGGGTGGCGCAAACCAAGGGGATTGCACGACTTGTTTGCGTCGCTAGCCGTGAGAAAACAAACAAGCAGGTGATCAACCAggg                                                                                                          >  2:466353/1‑150 (MQ=255)
                                         ccGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGCGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTgggg                                                                                                         <  2:859501/150‑1 (MQ=255)
                                         ccGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTgggg                                                                                                         <  2:219213/150‑1 (MQ=255)
                                             ccTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACg                                                                                                     >  1:145542/1‑150 (MQ=255)
                                               tttttCGGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTTCAAGACACCCATGCGGGTGGCGCAAACCAAGGGGTTTGCACGATGTGCTTGCTGCTGCCACCGGGAGAAGCAAAACAAGCAGGTAACCAACGTTGGGAACGCt                                                                                                   >  2:145306/1‑150 (MQ=255)
                                               tttttCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCACACGAGTGGGTGACCAAGCTGGGGAACGCt                                                                                                   >  1:642910/1‑150 (MQ=255)
                                                   tCCGTTATTGGTGTCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATg                                                                                               <  1:582329/150‑1 (MQ=255)
                                                   tCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATg                                                                                               >  1:683455/1‑150 (MQ=255)
                                                   tCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATg                                                                                               <  2:104053/150‑1 (MQ=255)
                                                      gTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCt                                                                                            >  1:671693/1‑150 (MQ=255)
                                                         aTTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTAc                                                                                         >  1:761162/1‑150 (MQ=255)
                                                               ggCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATac                                                                                   <  2:671693/150‑1 (MQ=255)
                                                                gCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATaca                                                                                  >  2:385295/1‑150 (MQ=255)
                                                                    gTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTga                                                                              <  2:751417/150‑1 (MQ=255)
                                                                         gCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGaaaa                                                                         >  1:736979/1‑150 (MQ=255)
                                                                         gCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGaaaa                                                                         >  1:892460/1‑150 (MQ=255)
                                                                             gTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTa                                                                                          <  2:382486/129‑1 (MQ=255)
                                                                             gTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTa                                                                                          >  1:382486/1‑129 (MQ=255)
                                                                                 cGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGGACGCTGATGGCTTACTCATACACTGAGCAAAAACGTATc                                                                 >  2:590608/1‑150 (MQ=255)
                                                                                    tAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGc                                                              <  1:1222749/150‑1 (MQ=255)
                                                                                         aGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGa                                                         >  2:54948/1‑150 (MQ=255)
                                                                                            tGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACtt                                                      >  1:604930/1‑150 (MQ=255)
                                                                                             gCAAGACACCCATGTGGGTGGCGCAAACCAAAGGGTTTGCACGATTTTCTGACTGCTCCCGGCGGGAGAAGCCAAACAAGCAGGTGACCAAGCGGGGCAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACttt                                                     <  1:466353/150‑1 (MQ=255)
                                                                                              cAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTa                                                    >  2:1095178/1‑150 (MQ=255)
                                                                                               aaGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGa                                                                                                 >  1:128882/1‑104 (MQ=255)
                                                                                               aaGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGa                                                                                                 <  2:128882/104‑1 (MQ=255)
                                                                                               aaGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAg                                                   >  1:1238220/1‑150 (MQ=255)
                                                                                                      ccATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAAc                                                                      <  1:574396/124‑1 (MQ=255)
                                                                                                      ccATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAAc                                                                      >  2:574396/1‑124 (MQ=255)
                                                                                                        aTGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGcc                                          >  1:169688/1‑150 (MQ=255)
                                                                                                         tgtgGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCg                                         <  1:1022420/150‑1 (MQ=255)
                                                                                                              gtggCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGt                                    <  2:312723/150‑1 (MQ=255)
                                                                                                               tggCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTc                                   <  1:891359/150‑1 (MQ=255)
                                                                                                                 gcgcAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATc                                                                 <  1:428835/118‑1 (MQ=255)
                                                                                                                 gcgcAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATc                                                                 >  2:428835/1‑118 (MQ=255)
                                                                                                                   gcAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATgg                               >  1:458532/1‑150 (MQ=255)
                                                                                                                        ccAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGAAGGACGTCAtagcatta                          >  2:913380/1‑143 (MQ=255)
                                                                                                                         cAAGGGGTTTGCACGCTTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGc                         <  2:892460/150‑1 (MQ=255)
                                                                                                                                    cACTATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTg              <  1:145306/150‑1 (MQ=255)
                                                                                                                                    cACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTg              <  2:604930/150‑1 (MQ=255)
                                                                                                                                    cACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTg              <  1:913380/150‑1 (MQ=255)
                                                                                                                                     aCGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTgg             >  2:824331/1‑150 (MQ=255)
                                                                                                                                       gATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTGGcc           >  1:14438/1‑150 (MQ=255)
                                                                                                                                       gATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTAATGGATGTGCCTTACCTCCTGGcc           >  2:642272/1‑150 (MQ=255)
                                                                                                                                        aTTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTGGCCa          >  1:120167/1‑150 (MQ=255)
                                                                                                                                              tGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGTGAAAAAACGTATCCGCAAGGTCTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTGGCCATCCAGc    <  2:145542/150‑1 (MQ=255)
                                                                                                                                                gctgctCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTGGCCATCCAGCTg  >  1:577055/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACCAAGCTGGGGAACGCTGATGGCTTACTCATACACTGAGAAAAAACGTATCCGCAAGGACTTTAGCAAGTTGCCGGACGTCATGGATGTGCCTTACCTCCTGGCCATCCAGCTG  >  NC_002947/537428‑537721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑