Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 558,260 C→A 7.3% T129T (ACC→ACA)  rplF → 50S ribosomal protein L6

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947558,2600CA 7.3% 172.8 / 2.1 55T129T (ACC→ACA) rplF50S ribosomal protein L6
Reads supporting (aligned to +/- strand):  ref base C (28/23);  new base A (2/2);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-01

CAAGGCTTCGAGCGCAAGCTCCAGCTGGTCGGTGTTGGTTACAAGGCACAGGCTAAAGGCACCGTCCTGAACCTGGCCCTGGGCTTCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTACAAAGGTAAAGGTGTGCGTTACGCGGACGAAGTAGTCCGTCGTAAAGAAGCCAAGAAGAAGTAGGG  >  NC_002947/558114‑558409
                                                                                                                                                  |                                                                                                                                                     
cAAGGCTTCGAGCGCAAGCTCCAGCTGGTCGGTGTTGGTTACAAGGCACAGGCTAAAGGCACCGTCCTGAACCTGGCCCTGGGCTTCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGAc                                                                                                                                                    >  1:500225/1‑150 (MQ=255)
 aaGGCTTCGAGCGCAAGCTCCAGCTGGTCGGTGTTGGTTACAAGGCACAGGCTAAAGGCACCGTCCTGAACCTGGCCCTGGGCTTCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACa                                                                                                                                                   >  1:257048/1‑150 (MQ=255)
  aGGCTTCGAGCGCAAGCTCCAGCTGGTCGGTGTTGGTTACAAGGCACAGGCTAAAGGCACAGTCCTGAACCTGGCCCTGGGCTTCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACAt                                                                                                                                                  <  1:544833/150‑1 (MQ=255)
        cGAGCGCAAGCTCCAGCTGGTCGGTGTTGGTTACAAGGCACAGGCTAAAGGCACCGTCCTGAACCTGGCCCTGGGCTTCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGAt                                                                                                                                            <  1:705496/150‑1 (MQ=255)
           gcgcAAGCTCCAGCTGGTCGGTGTTGGTTACAAGGCACAGGCTAAAGGCACCGTCCTGAACCTGGCCCTGGGCTTCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCaa                                                                                                                                         <  1:113550/150‑1 (MQ=255)
             gcAAGCTCCAGCTGGTCGGTGTTGGTTACAAGGCACAGGCTAAAGGCACCGTCCTGAACCTGGCCCTGGGCTTCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAgg                                                                                                                                       <  1:876178/150‑1 (MQ=255)
             gcAAGCTCCAGCTGGTCGGTGTTGGTTACAAGGCACAGGCTAAAGGCACCGTCCTGAACCTGGCCCTGGGCTTCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAgg                                                                                                                                       <  1:828099/150‑1 (MQ=255)
                  ctctAGCTGGTCGGTGTTGGTTACAAGGCACAGGCTAAAGGCACCGTCCTGAACCTGGCCCTGGGCTTCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATc                                                                                                                                  <  2:253756/150‑1 (MQ=255)
                         tGGTCGGTGTTGGTTACAAGGCACAGGCTAAAGGCACCGTCCTGAACCTGGCCCTGGGCTTCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAagc                                                                                                                           <  1:313685/150‑1 (MQ=255)
                                     gTTACAAGGCACAGGCTAAAGGCACCGTCCTGAACCTGGCCCTGGGCTTCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTc                                                                                                               >  2:152494/1‑150 (MQ=255)
                                                caGGCTAAAGGCACCGTCCTGAACCTGGCCCTGGGCTTCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCt                                                                                                    >  2:57734/1‑150 (MQ=255)
                                                 aGGCTAAAGGCACCGTCCTGAACCTGGCCCTGGGCTTCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTg                                                                                                   <  2:1282446/150‑1 (MQ=255)
                                                   gCTAAAGGCACCGTCCTGAACCTGGCCCTGGGCTTCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATc                                                                                                                                  >  1:1073685/1‑117 (MQ=255)
                                                   gCTAAAGGCACCGTCCTGAACCTGGCCCTGGGCTTCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATc                                                                                                                                  <  2:1073685/117‑1 (MQ=255)
                                                         ggCACCGTCCTGAACCTGGCCCTGGGCTTCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCcgc                                                                                           >  2:490553/1‑150 (MQ=255)
                                                            aCCGTCCTGAACCTGGCCCTGGGCTTCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGAc                                                                                        >  2:951183/1‑150 (MQ=255)
                                                                 ccTGAACCTGGCCCTGGGCTTCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTg                                                                                                                      <  2:1203421/115‑1 (MQ=255)
                                                                 ccTGAACCTGGCCCTGGGCTTCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTg                                                                                                                      >  1:1203421/1‑115 (MQ=255)
                                                                       ccTGGCCCTGGGCTTCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGcc                                                                             >  2:39373/1‑150 (MQ=255)
                                                                             ccTGGGCTTCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACaa                                                                                                                             >  1:731655/1‑96 (MQ=255)
                                                                             ccTGGGCTTCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACaa                                                                                                                             <  2:731655/96‑1 (MQ=255)
                                                                                 ggCTTCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTAc                                                                   <  1:57734/150‑1 (MQ=255)
                                                                                 ggCTTCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTAc                                                                   <  1:152494/150‑1 (MQ=255)
                                                                                 ggCTTCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACAGACATCCTGATCAAGGGTATAGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGaaa                                                                                                >  1:1052813/1‑121 (MQ=255)
                                                                                 ggCTTCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACAGACATCCTGATCAAGGGTATAGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGaaa                                                                                                <  2:1052813/121‑1 (MQ=255)
                                                                                  gCTTCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTACa                                                                  <  1:490553/150‑1 (MQ=255)
                                                                                   cTTCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTACaa                                                                 >  2:326035/1‑150 (MQ=255)
                                                                                    ttCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTACaaa                                                                <  1:326035/150‑1 (MQ=255)
                                                                                           aCCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAg                                                                                                                                        >  2:173528/1‑71 (MQ=255)
                                                                                           aCCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAg                                                                                                                                        <  1:173528/71‑1 (MQ=255)
                                                                                           aCCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTACaa                                                                 <  2:981665/142‑1 (MQ=255)
                                                                                           aCCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTACaa                                                                 <  2:981771/142‑1 (MQ=255)
                                                                                           aCCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTACaa                                                                 >  1:981665/1‑142 (MQ=255)
                                                                                           aCCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTACaa                                                                 >  1:981771/1‑142 (MQ=255)
                                                                                            cccAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACAGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTACaaaggtaaagg                                                        >  1:216451/1‑150 (MQ=255)
                                                                                             ccAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAgg                                                                                                                                       >  1:1092814/1‑70 (MQ=255)
                                                                                             ccAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAgg                                                                                                                                       <  2:1092814/70‑1 (MQ=255)
                                                                                             ccAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACAGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTACaaaggtaaaggt                                                       <  2:216451/150‑1 (MQ=255)
                                                                                                    aCTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTACAAAGGTAAAGGtgtg                                                    >  1:598476/1‑146 (MQ=255)
                                                                                                    aCTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTACAAAGGTAAAGGtgtg                                                    <  2:598476/146‑1 (MQ=255)
                                                                                                          aaCTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAAGAGCACAGGTCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCATTAAAAAGGTAGAGGTGTGCGTTACGCgg                                          >  1:720267/1‑150 (MQ=255)
                                                                                                            cTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTACAAAGGTAAAGGTGTGCGTTACGCGGAc                                        >  2:335250/1‑150 (MQ=255)
                                                                                                            cTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTACAAAGGTAAAGGTGTGCGTTACGCGGAc                                        >  1:332420/1‑150 (MQ=255)
                                                                                                                cAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCTACTTCCGTCCGCCAGAGCCTTACAAAGGTAAAGGTGTGCGTTACGCGGACGAag                                    <  2:500225/150‑1 (MQ=255)
                                                                                                                   ccGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTACAAAGGTAAAGGTGTGCGTTACGCGGACGAagtag                                 >  2:1208788/1‑150 (MQ=255)
                                                                                                                         tCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTACAAAGGTAAAGGTGTGAGTTACGCGGACGAAGTAGTCcgtc                           <  1:39373/150‑1 (MQ=255)
                                                                                                                          cACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTACAAAGGTAAAGGTGTGCGTTACGCGGACGAAGTAGTCcgtcg                          >  1:901613/1‑150 (MQ=255)
                                                                                                                           aCCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTACAAAGGTAAAGGTGTGCGTTACGCGGACGAag                                    >  2:1236447/1‑139 (MQ=255)
                                                                                                                           aCCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTACAAAGGTAAAGGTGTGCGTTACGCGGACGAag                                    <  1:1236447/139‑1 (MQ=255)
                                                                                                                           aCCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTACAAAGGTAAAGGTGTGCGTTACGCGGACGAAGTAGTCcgtcgt                         >  1:523462/1‑150 (MQ=255)
                                                                                                                            ccGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTACAAAGGTAAAGGTGTGCGTTACGCGGACGAAGTAGTCCGTCGTa                        >  2:153106/1‑150 (MQ=255)
                                                                                                                                    acacCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTACAAAGGTAAAGGTGTGCGTTACGCGGACGAAGTAGTCCGTCGTAAAGAAGcc                >  2:1230595/1‑150 (MQ=255)
                                                                                                                                           gCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTACAAAGGTAAAGGTGTGCGTTACGCGGACGAAGTAGTCCGTCGTAAAGAAGCCaagaaga         >  1:61802/1‑150 (MQ=255)
                                                                                                                                              aGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTACAAAGGTAAAGGTGTGCGTTACGCGGACGAAGTAGTCCGTCGTAAAGAAGCCAAGAAGAAGt      >  1:1014931/1‑150 (MQ=255)
                                                                                                                                                aCCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTACAAAGGTAAAGGTGTGCGTTACGCGGACGAAGTAGTCCGTCGTAAAGAAGCCAAGAAGAAGTAg    >  2:686857/1‑150 (MQ=255)
                                                                                                                                                 ccGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTACAAAGGTAAAGGTGTGCGTTACGCGGACGAAGTAGTCCGTCGTAAAGAAGCCAAGAAGAAGTAgg   <  1:335250/150‑1 (MQ=255)
                                                                                                                                                 ccGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTACAAAGGTAAAGGTGTGCGTTACGCGGACGAAGTAGTCCGTCGTAAAGAAGCCAAGAAGAAGTAgg   <  2:61802/150‑1 (MQ=255)
                                                                                                                                                  cGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTACAAAGGTAAAGGTGTGCGTTACGCGGACGAAGTAGTCCGTCGTAAAGAAGCCAAGAAGAAGTAggg  >  1:555935/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
CAAGGCTTCGAGCGCAAGCTCCAGCTGGTCGGTGTTGGTTACAAGGCACAGGCTAAAGGCACCGTCCTGAACCTGGCCCTGGGCTTCTCTCACCCAGTGGACTACGAACTGCCAGCCGGTATCACCGCTGAAACACCTAGCCAGACCGACATCCTGATCAAGGGTATCGACAAGCAGCTGGTAGGTCAGGTGGCCGCTGAAATCCGCGACTTCCGTCCGCCAGAGCCTTACAAAGGTAAAGGTGTGCGTTACGCGGACGAAGTAGTCCGTCGTAAAGAAGCCAAGAAGAAGTAGGG  >  NC_002947/558114‑558409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑