Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 648,883 C→T 7.2% A266A (GCG→GCA)  accC ← acetyl‑CoA carboxylase biotin carboxylase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947648,8830CT 7.2% 156.4 / 2.4 55A266A (GCG→GCA) accCacetyl‑CoA carboxylase biotin carboxylase subunit
Reads supporting (aligned to +/- strand):  ref base C (27/24);  new base T (2/2);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

AGCATCTCCTTGACGATGTCGATACCAGTGACCATCTCCGACACCGGGTGCTCAACCTGCACGCGGGTGTTCATCTCGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCGGGGCTGGTGCTTCTTCCAGTACTTTCTGGTGACGGCGCTGCAGCGAGCAGTCGCGGTCGCCCAGGTGAATGGCGTTGCCCTGGCCGTCGGACAGTAC  >  NC_002947/648734‑649032
                                                                                                                                                     |                                                                                                                                                     
aGCATCTCCTTGACGATGTCGATACCAGTGACCATCTCCGACACCGGGTGCTCAACCTGCACGCGGGTTTTCATCTCGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCAc                                                                                                                                                       <  1:1124019/150‑1 (MQ=255)
aGCATCTCCTTGACGATGTCGATACCAGTGACCATCTCCGACACCGGGTGCTCAACCTGCACGCGGGTGTTCATCTCGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCAc                                                                                                                                                       <  1:1143049/150‑1 (MQ=255)
 gCATCTCCTTGACGATGTAGATACCAGTGACCATCTCCGACACCGGGTGCTCAACCTGCACGCGGGTGTTCATCTCGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACg                                                                                                                                                      >  1:682124/1‑150 (MQ=255)
      tcCTTGACGATGTCGATACCAGTGACCATCTCCGACACCGGGTGCTCAACCTGCACGCGGGTGTTCATCTCGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCg                                                                                                                                                 >  1:150686/1‑150 (MQ=255)
               aTGTCGATACCAGTGACCATCTCCGACACCGGGTGCTCAACCTGCACGCGGGTGTTCATCTCGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAgcgc                                                                                                                                        <  2:1071028/150‑1 (MQ=255)
                tGTCGATACCAGTGACCATCTCCGACACCGGGCGCTCAACCTGCACGCGGGTGTTCATCTCGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCt                                                                                                                                       >  2:1096213/1‑150 (MQ=255)
                    gATACCAGTGACCATCTCCGACACCGGGTGCTCAACCTGCACGCGGGTGTTCATCTCGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGaa                                                                                                                                   >  2:280109/1‑150 (MQ=255)
                    gATACCAGTGACCATCTCCGACACCGGGTGCTCAACCTGCACGCGGGTGTTCATCTCGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGaa                                                                                                                                   >  1:838744/1‑150 (MQ=255)
                         cAGTGACCATCTCCGACACCGGGTGCTCAACCTGCACGCGGGTGTTCATCTCGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACtt                                                                                                                              >  1:216178/1‑150 (MQ=255)
                          aGTGACCATCTCCGACACCGGGTGCTCAACCTGCACGCGGGTGTTCATCTCGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTc                                                                                                                             >  2:1054147/1‑150 (MQ=255)
                              aCCATCTCCGACACCGGGTGCTCAACCTGCACGCGGGTGTTCATCTCGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCt                                                                                                                                       >  1:1257555/1‑136 (MQ=255)
                              aCCATCTCCGACACCGGGTGCTCAACCTGCACGCGGGTGTTCATCTCGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCt                                                                                                                                       <  2:1257555/136‑1 (MQ=255)
                                   ctcCGACACCGGGTGCTCAACCTGCACGCGGGTGTTCATCTCGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCa                                                                                                                                            <  1:945184/126‑1 (MQ=255)
                                   ctcCGACACCGGGTGCTCAACCTGCACGCGGGTGTTCATCTCGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCa                                                                                                                                            >  2:945184/1‑126 (MQ=255)
                                      cGACACCGGGTGCTCAACCTGCACGCGGGTGTTCATCTCGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCttt                                                                                                                 >  1:629841/1‑150 (MQ=255)
                                           ccGGGTGCTCAACCTGCACGCGGGTGTTCATCTCGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCAt                                                                                                            <  2:216178/150‑1 (MQ=255)
                                                tGCTCAACCTGCACGCGGGTGTTCATCTCGATGAAGTAGAAGCGCCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATg                                                                                                       <  1:326655/150‑1 (MQ=255)
                                                   tCAACCTGCACGCGGGTGTTCATCTCGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGccc                                                                                                    >  2:587269/1‑150 (MQ=255)
                                                   tCAACCTGCACGCGGGTGTTCATCTCGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGccc                                                                                                    >  2:822497/1‑150 (MQ=255)
                                                   tCAACCTGCACGCGGGTGTTCATCTCGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGccc                                                                                                    >  2:92950/1‑150 (MQ=255)
                                                     aaCCTGCACGCGGGTGTTCATCTCGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCgg                                                                                                  >  2:1186597/1‑150 (MQ=255)
                                                            aCGCGGGTGTTCATCTCGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCttt                                                                                                                 <  2:1201096/128‑1 (MQ=255)
                                                            aCGCGGGTGTTCATCTCGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCttt                                                                                                                 >  1:1201096/1‑128 (MQ=255)
                                                              gcgGGTGTTCATCTCGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCGGGGCTGGTGc                                                                                         <  1:1126439/150‑1 (MQ=255)
                                                                    gtTCATCTCGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCGGGGCTGGTGCttcttc                                                                                   <  1:452236/150‑1 (MQ=255)
                                                                        aTCTCGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCGTGCATCGagat                                                                                                                                             >  1:1065908/1‑85 (MQ=255)
                                                                        aTCTCGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCGTGCATCGagat                                                                                                                                             <  2:1065908/88‑4 (MQ=255)
                                                                        aTCTCGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCGGGGCTGGTGCTTCTTCCAGt                                                                               <  1:280109/150‑1 (MQ=255)
                                                                         tctcGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTc                                                                                                                             <  2:169713/103‑1 (MQ=255)
                                                                         tctcGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTc                                                                                                                             >  1:169713/1‑103 (MQ=255)
                                                                                   gTAGAAGCGGCCGTTTTCGTACAGGAACTCGACCGTATGCCCAACACGGTAGCGAATCTTGAGGCACGGGACGATGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCGGGGCTGGTGCTTCTTCCAGTACTTTCTGGTg                                                                    >  1:349483/1‑150 (MQ=255)
                                                                                      gAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCGGGGCTGGTGCTTCTTCCAGTACTTTCTGGTGACg                                                                 <  2:838744/150‑1 (MQ=255)
                                                                                          cGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCGGGGCTGGTGCTTCTTCCAGTACTTTCTGGTGACGgcgc                                                             <  1:1096213/150‑1 (MQ=255)
                                                                                             ccGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCGGGGCTGGTGCTTCTTCCAGTACTTTCTGGTGACGGCGCTGc                                                          <  2:682124/150‑1 (MQ=255)
                                                                                                     gTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCGGGGCTGGTGCTTCTTCCAGTACTTTCTg                                                                       >  2:389767/1‑129 (MQ=255)
                                                                                                     gTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCGGGGCTGGTGCTTCTTCCAGTACTTTCTg                                                                       <  1:389767/129‑1 (MQ=255)
                                                                                                      tACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCg                                                                                                   >  2:432017/1‑100 (MQ=255)
                                                                                                      tACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCg                                                                                                   <  1:432017/100‑1 (MQ=255)
                                                                                                      tACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCGGGGCTGGTGCTTCTTCCAGTACTTTCTGGTGACGGCGCTGCAGCGAGCAg                                                 >  2:255347/1‑150 (MQ=255)
                                                                                                       aCAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCGGGGCTGGTGCTTCTTCCAGTACTTTCTGGTGACGGCGCTGCAGCGAGCAGt                                                >  1:62180/1‑150 (MQ=255)
                                                                                                           gAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCGGGGCTGGTGCTTCTTCCAGTACTTTCTGGTGACGGCGCTGCAGCGAGCAGTcgcg                                            >  2:448285/1‑150 (MQ=255)
                                                                                                            aaCTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCGGGGCTGGTGCTTCTTCCAGTACTTTCTGGTGACGGCGCTGCAGCGAGCAGTCGCgg                                           <  1:150983/150‑1 (MQ=255)
                                                                                                               tCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCGGGGCTGGt                                                                                           >  1:248026/1‑99 (MQ=255)
                                                                                                               tCGAAAGTACCGGCACCACGGTAGCCCATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCGGGGCTGGt                                                                                           <  2:248026/99‑1 (MQ=255)
                                                                                                                        ccGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCGGGGCTGGTGCTTCTTCCAGTACTTTCTGGTGACGGCGCTGCAGCGAGCAGTCGCGGTCGCCCAGGTGa                               >  2:1204474/1‑150 (MQ=255)
                                                                                                                           gCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCGGGGCTGGTGCTTCTTCCAGTACTTTCTGGTGACGGCGCTGCAGCGAGCAGTCGCGGTCGCCCAGGTGAATg                            <  2:134506/150‑1 (MQ=255)
                                                                                                                                       ccGATCTCGATGCACGCATCGACGCAGCGCTTGAACCCGTCCTGACGTGCTTTCTCATCGATGCCCGGGGCAGGTGCTTCTTCCAGTACTTTCTGGTGACGGCGCTGCAGCGAGCAGTCGCGGTCGCCCAGGTGAATGGCGTTGCCCTgg                <  1:85510/150‑1 (MQ=255)
                                                                                                                                           tctcGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGccc                                                                                                    >  2:1281272/1‑62 (MQ=255)
                                                                                                                                           tctcGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGccc                                                                                                    <  1:1281272/62‑1 (MQ=255)
                                                                                                                                           tctcGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCGGGGCTGGTGc                                                                                         <  1:724734/73‑1 (MQ=255)
                                                                                                                                           tctcGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCGGGGCTGGTGc                                                                                         >  2:724734/1‑73 (MQ=255)
                                                                                                                                            gtcGATGCGTGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCGGGGCTGGTGCTTCTTCCAGTACTTTCTGGTGACGGCGCTGCAGCGAGCAGTcg                                              <  2:170464/114‑1 (MQ=255)
                                                                                                                                            gtcGATGCGTGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCGGGGCTGGTGCTTCTTCCAGTACTTTCTGGTGACGGCGCTGCAGCGAGCAGTcg                                              >  1:170464/2‑115 (MQ=255)
                                                                                                                                            ctcGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCGGGGCTGGTGCTTCTTCCAGTACTTTCTGGTGACGGCGCTGCAGCGAGCAGTCGCGGTCGCCCAGGTGAATGGCGTTGCCCTGGCCGTc           <  1:448285/150‑1 (MQ=255)
                                                                                                                                            ctcGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCGGGGCTGGTGCTTCTTCCAGTACTTTCTGGTGACGGCGCTGCAGCGAGCAGTCGCGGTCGCCCAGGTGAATGGCGTTGCCCTGGCCGTc           <  1:92950/150‑1 (MQ=255)
                                                                                                                                                  gCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCGGGGCTGGTGCTTCTTCCAGTACTTTCTGGTGACGGCGCTGCAGCGAGCAGTCGCGGTCGCCCAGGTGAATGGCGTTGCCCTGGCCGTCGGACAg     <  1:822497/150‑1 (MQ=255)
                                                                                                                                                    aCGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCGGGGCTGGTGCTTCTTCCAGTACTTTCTGGTGACGGCGCTGCAGCGAGCAGTCGCGGTCGCCCAGGTGAATGGCGTTGCCCTGGCCGTCGGACAGTa   >  1:1270312/1‑150 (MQ=255)
                                                                                                                                                     cGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCGGGGCTGGTGCTTCTTCCAGTACTTTCTGGTGACGGCGCTGCAGCGAGCAGTCGCGGTCGCCCAGGTGAATGGCGTTGCCCTGGCCGTCGGACAGTAc  <  1:1204474/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGCATCTCCTTGACGATGTCGATACCAGTGACCATCTCCGACACCGGGTGCTCAACCTGCACGCGGGTGTTCATCTCGATGAAGTAGAAGCGGCCGTTTTCGTACAGGAACTCGAAAGTACCGGCACCACGGTAGCCGATCTCGATGCACGCATCGACGCAGCGCTTGAACACTTCCTGACGGGCTTTCTCATCGATGCCCGGGGCTGGTGCTTCTTCCAGTACTTTCTGGTGACGGCGCTGCAGCGAGCAGTCGCGGTCGCCCAGGTGAATGGCGTTGCCCTGGCCGTCGGACAGTAC  >  NC_002947/648734‑649032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑