| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 733,852 | C→T | 8.0% | intergenic (+19/+172) | PP_t11 → / ← ndh | tRNA‑Lys/NADH dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 733,852 | 0 | C | T | 8.0% | 170.0 / 2.6 | 59 | intergenic (+19/+172) | PP_t11/ndh | tRNA‑Lys/NADH dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (29/24); new base T (2/3); total (32/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAATGCGGCAGTGCAGCCACCATCGCATTCACAGGTAAACCCG > NC_002947/733712‑733998 | ggAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGc < 1:324041/150‑1 (MQ=255) gATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCttttt > 2:618366/1‑150 (MQ=255) gTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCtt > 2:94910/1‑150 (MQ=255) gTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCtt > 1:433646/1‑150 (MQ=255) cAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCtttt < 1:94910/150‑1 (MQ=255) ggAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGc < 1:772483/150‑1 (MQ=255) gAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGcc > 2:857235/1‑150 (MQ=255) ttCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCg > 2:556735/1‑150 (MQ=255) ttCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCATGCGAGATGCCCtttg > 2:1189263/1‑137 (MQ=14) ttCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCATGCGAGATGCCCtttg < 1:1189263/138‑2 (MQ=14) gAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGaaa > 1:395019/1‑150 (MQ=255) aaTCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGaaaa > 1:525576/1‑150 (MQ=255) cccTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATgc < 1:1010821/150‑1 (MQ=255) cttaaCCCCGATCATTTGTGGGTCGTTAGCTCATTTGTTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAACCCTACACTACCCACCATGAACAAAGGGCATCTCGTATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATgcg < 2:351366/146‑1 (MQ=21) ccTCACCCCGACCATTTTTTTTTTGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATgcg > 2:933773/1‑150 (MQ=255) cTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGcc > 2:940516/1‑123 (MQ=255) cTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGcc < 1:940516/123‑1 (MQ=255) ccGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCg < 2:433646/150‑1 (MQ=255) aCCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCtttt < 2:250220/125‑1 (MQ=255) aCCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCtttt > 1:250220/1‑125 (MQ=255) aTTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCaa < 1:857235/150‑1 (MQ=255) gTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCt > 2:635625/1‑150 (MQ=255) aGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAACAATGCGCCCCTCGCCGCAATCCCACCTGCATCCATTTg > 2:27365/1‑150 (MQ=255) gTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGGCCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGc > 1:91623/1‑150 (MQ=255) gTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGc < 2:525576/150‑1 (MQ=255) tGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCca < 2:127795/150‑1 (MQ=255) gTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCttt < 1:522474/97‑1 (MQ=255) gTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCttt > 2:522474/1‑97 (MQ=255) aGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGAATGAGATGCCCTTTTTCTTTTGCCTCGAAACATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGccgc > 1:820453/1‑148 (MQ=255) ttttAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGc < 2:574630/132‑1 (MQ=255) ttttAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGc > 1:574630/1‑132 (MQ=255) ttttAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAg > 1:1056642/1‑150 (MQ=255) tttAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCAGCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAgg < 2:310300/150‑1 (MQ=255) ccAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTAttt < 1:1011836/124‑1 (MQ=255) ccAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTAttt > 2:1011836/1‑124 (MQ=255) ccAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCgg > 1:1151173/1‑150 (MQ=255) cAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCggg > 2:238290/1‑150 (MQ=255) cAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCggg < 2:1151173/150‑1 (MQ=255) cAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCggg < 1:933773/150‑1 (MQ=255) tGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCa < 1:618366/150‑1 (MQ=255) gTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCAcc > 2:1063056/1‑106 (MQ=255) gTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCAcc < 1:1063056/106‑1 (MQ=255) tCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCAccc > 2:501304/1‑150 (MQ=255) tCGTAGGTGCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCAGCCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCAccc < 1:27365/150‑1 (MQ=255) ttCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAATg > 1:86821/1‑150 (MQ=255) tCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAATGc > 2:361800/1‑150 (MQ=255) cGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAATGCg < 2:86821/150‑1 (MQ=255) ccaccaTGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCCTCGGCGCAATCCCAACTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAATGc < 1:349481/134‑1 (MQ=255) ccaccaTGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAATGc > 2:349481/1‑134 (MQ=255) ccaccaTGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAATGCGGCAGTGCAGccacca > 1:387128/1‑150 (MQ=255) accaTGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAATGCGGCAGTGCAGCCACCATc < 2:387128/150‑1 (MQ=255) accaTGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAATGCGGCAGTGCAGCCACCATc > 1:814559/1‑150 (MQ=255) accaTGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAATGCGGCAGTGCAGCCACCATc > 1:493339/1‑150 (MQ=255) accaTGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAATGCGGCAGTGCAGCCACCATc < 2:1056642/150‑1 (MQ=255) tGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAATGCGGCAGTGCAGCCACCATCGCAt < 2:493339/150‑1 (MQ=255) gaaaaaGGGCATCTCATGCGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCCATGTAGGAGCGGGTTCACCCGCGAATGCGGCAGTGCAGCCACCATc > 2:95284/4‑144 (MQ=255) gaaaaaGGGCATCTCATGCGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCCATGTAGGAGCGGGTTCACCCGCGAATGCGGCAGTGCAGCCACCATc < 1:95284/141‑1 (MQ=255) ggCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAATGCGGCAGTGCAGCCACCATCGCATTCACAGGTa > 2:631274/1‑150 (MQ=255) tcGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTGGCCCGCGAATGCGGCAGTGCAGCCACCGTCGCGTTGGCAGGTAAACCcg > 1:89054/1‑150 (MQ=255) | GGAGCAGGATGTCAGGAGTTCGAATCCCCTCACCCCGACCATTTTTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGGCTTTTAACCAATTGGTCGTAGGTTCGAATCCTACACGACCCACCATGAACAAAGGGCATCTCGCATGAGATGCCCTTTTTCTTTTGCCTCGAAAAATGCGCCCATCGGCGCAATCCCACCTGCATCTATTTGCCACACCGCCCCTATGTAGGAGCGGGTTCACCCGCGAATGCGGCAGTGCAGCCACCATCGCATTCACAGGTAAACCCG > NC_002947/733712‑733998 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |