Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 798,632 T→A 7.7% L201Q (CTG→CAG)  fklB‑I → FKBP‑type peptidyl‑prolyl cis‑trans isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947798,6320TA 7.7% 145.4 / 3.6 52L201Q (CTG→CAG) fklB‑IFKBP‑type peptidyl‑prolyl cis‑trans isomerase
Reads supporting (aligned to +/- strand):  ref base T (26/22);  new base A (2/2);  total (28/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

ATCGCTGGCTGGACCGAAGCCCTGCAGCTGATGAACGCTGGCAGCAAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAGTTCCTTGAGCACCCCCGGTTCACTCGAATTCAGCCC  >  NC_002947/798493‑798779
                                                                                                                                           |                                                                                                                                                   
aTCGCTGGCTGGACCGAAGCCCTGCAGCTGATGAACGCTGGCAGCAAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGtt                                                                                                                                           <  1:469092/150‑1 (MQ=255)
aTCGCTGGCTGGACCGAAGCCCTGCAGCTGATGAACGCTGGCAGCAAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCACAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGtt                                                                                                                                           >  2:1217611/1‑150 (MQ=255)
    ctggctggACCGAAGCCCTGCAGCTGATGAACGCTGGCAGCAAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGt                                                                                                                                       <  2:496481/150‑1 (MQ=255)
         tggACCGAAGCCCTGCAGCTGATGAACGCTGGCAGCAAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGc                                                                                                                                  >  2:867220/1‑150 (MQ=255)
                aaGCCCTGCAGCTGATGAACGCTGGCAGCAAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAg                                                                                                                           <  1:310246/150‑1 (MQ=255)
                 aGCCCTGCAGCTGATGAACGCTGGCAGCAAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAgg                                                                                                                          >  1:1126010/1‑150 (MQ=255)
                    ccTGCAGCTGATGAACGCTGGCAGCAAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAggccg                                                                                                                       >  1:1123012/1‑150 (MQ=255)
                       gCAGCTGATGAACGCTGGCAGCAAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGAACAGCGTTCTGCTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGGggccggcc                                                                                                                    <  2:1241871/150‑1 (MQ=255)
                       gCAGCTGATCAACGCTGGCAGCAAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAggccggcc                                                                                                                    <  1:838570/150‑1 (MQ=255)
                         aGCTGATGAACGCTGGCAGCAAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCt                                                                                                                  >  2:576458/1‑150 (MQ=255)
                                 aaCGCTGGCAGCAAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCt                                                                                                          <  1:867220/150‑1 (MQ=255)
                                  aCGCTGGCAGCAAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAAt                                                                                                                                    <  1:21344/123‑1 (MQ=255)
                                  aCGCTGGCAGCAAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAAt                                                                                                                                    >  2:21344/1‑123 (MQ=255)
                                   cGCTGGCAGCAAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCa                                                                                                        >  1:271963/1‑150 (MQ=255)
                                        gcagcaAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGTCGGCCCTGCCGGCCTCAATTcc                                                                                                   >  2:1155414/1‑150 (MQ=255)
                                          agcaAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAggccgg                                                                                                                      >  2:1200478/1‑129 (MQ=255)
                                          agcaAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAggccgg                                                                                                                      <  1:1200478/129‑1 (MQ=255)
                                          agcaAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCCAg                                                                                                 >  1:990408/1‑150 (MQ=255)
                                           gcaAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCCAGt                                                                                                <  2:861440/150‑1 (MQ=255)
                                            caAATGGCGCCTGTACGTGCCAAGCGAGCTGACTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGAACAGCGCACATGTGTTCGACGTCGAGCTGCTCGACGTTATGTAGTACCCTGGAGGCCTGCCCTGCCAGCCTCAATTCCAGTc                                                                                               >  1:724809/1‑150 (MQ=255)
                                                   cgcCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCCAGTCTGTcccc                                                                                        <  2:990408/150‑1 (MQ=255)
                                                         tACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCCAGTCTGTCCCCCCAggg                                                                                  >  1:138644/1‑150 (MQ=255)
                                                          aCGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCCAGTCTGTCCCCCCAGGgc                                                                                 >  2:1106531/1‑150 (MQ=255)
                                                                 aaGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTc                                                                                                                                          >  1:1084785/1‑86 (MQ=255)
                                                                 aaGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTc                                                                                                                                          <  2:1084785/86‑1 (MQ=255)
                                                                 aaGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGc                                                                          <  2:734283/150‑1 (MQ=255)
                                                                         tGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCCAGTCTGTc                                                                                           <  2:368714/125‑1 (MQ=255)
                                                                         tGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCCAGTCTGTc                                                                                           >  1:368714/1‑125 (MQ=255)
                                                                         tGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCCAGTCTGTCCCCCCGGGTCGCAACGCCCGCGCAt                                                                  >  2:615792/1‑150 (MQ=255)
                                                                           gCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTc                                                                                                    <  1:649000/114‑1 (MQ=255)
                                                                           gCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTc                                                                                                    >  2:649000/1‑114 (MQ=255)
                                                                             ttACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGca                                                              <  2:138644/150‑1 (MQ=255)
                                                                                 ggCGCCCAAGGCGTTGGTAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCGGCCGGCCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGcagaac                                                          <  1:199254/150‑1 (MQ=255)
                                                                                 ggCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGcataac                                                          >  1:59794/1‑150 (MQ=255)
                                                                                   ggcCCAAGGCGTTGGCAGCATCTCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTCCCGGCCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGcagaacag                                                        <  2:724809/149‑1 (MQ=255)
                                                                                    gcCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGcagaacaga                                                       >  1:140323/1‑150 (MQ=255)
                                                                                      ccAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAGaacagaaa                                                     >  2:747046/1‑150 (MQ=255)
                                                                                                     caTCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAg                                      >  1:231810/1‑150 (MQ=255)
                                                                                                             cgcACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAGTTCCTTGa                              >  2:499470/1‑150 (MQ=255)
                                                                                                             cgcACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAGTTCCTTGa                              >  2:102241/1‑150 (MQ=255)
                                                                                                             cgcACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAAAACAGAAACAGATTCCGTACCAGTTCCTTGa                              <  1:1191079/150‑1 (MQ=255)
                                                                                                                acaGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCCAGTCTGTCCCCCCAgg                                                                                   >  1:560155/1‑94 (MQ=255)
                                                                                                                acaGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCCAGTCTGTCCCCCCAgg                                                                                   <  2:560155/94‑1 (MQ=255)
                                                                                                                      ttCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAGTTCCTTGAGc                            <  2:569300/143‑1 (MQ=255)
                                                                                                                      ttCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAGTTCCTTGAGc                            >  1:569300/1‑143 (MQ=255)
                                                                                                                         tGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAGTTCCTTGAGCACCCCCGGtt                  <  2:1002829/150‑1 (MQ=255)
                                                                                                                            tacagaaCGTCGAGCAGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAGTTCCTTGAGCACCCCCGGt                   >  1:437365/7‑146 (MQ=255)
                                                                                                                            tacagaaCGTCGAGCAGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAGTTCCTTGAGCACCCCCGGt                   <  2:437365/140‑1 (MQ=255)
                                                                                                                              ttCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGTCGGCCCTGCCGGCCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAGTTCCTTGAGCACCCCCGGTTCACTc             <  1:1155414/150‑1 (MQ=255)
                                                                                                                                    gTCGAGCAGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAGTTCCTTGa                              <  2:353072/127‑1 (MQ=255)
                                                                                                                                    gTCGAGCAGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAGTTCCTTGa                              >  1:353072/1‑127 (MQ=255)
                                                                                                                                      cGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCAAGACCGGCCCCCCAGGGGTCTGCGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAGTTCCTTGAGCACCCCCGGTTCACTCGAATTCAg     >  1:157025/1‑150 (MQ=255)
                                                                                                                                         gctgctCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAGTTCCTTGAGCACCCCCGGTTCACTCGAATTCAGccc  >  2:1150511/1‑150 (MQ=255)
                                                                                                                                           |                                                                                                                                                   
ATCGCTGGCTGGACCGAAGCCCTGCAGCTGATGAACGCTGGCAGCAAATGGCGCCTGTACGTGCCAAGCGAGCTGGCTTACGGCGCCCAAGGCGTTGGCAGCATCCCGCCGCACAGCGTTCTGGTGTTCGACGTCGAGCTGCTCGACGTTCTGTAATGCCCTGGAGGCCGGCCCTGCCGGCCTCAATTCCAGTCTGTCCCCCCAGGGCGCAACGCCCGCGCATAGCAGAACAGAAACAGATTCCGTACCAGTTCCTTGAGCACCCCCGGTTCACTCGAATTCAGCCC  >  NC_002947/798493‑798779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑