Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	799,850	A→T	10.8%	L209Q (CTG→CAG)	ispB ←	octaprenyl diphosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	799,850	0	A	T	10.8%	188.0 / 13.8	65	L209Q (CTG→CAG)	ispB	octaprenyl diphosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (30/28);  new base T (5/2);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.34e-01

CCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCCGCGAATGACCTCCATGTAGACTTCCTCG  >  NC_002947/799704‑799999                                                                                                                                                   |                                                                                                                                                      ccTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCt                                                                                                                                                    >  2:1045464/1‑150 (MQ=255)     gCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCTGGTCGACGACCTGCTGGac                                                                                                                                                <  1:174740/150‑2 (MQ=255)       gTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAgg                                                                                                                                              >  2:160013/1‑150 (MQ=255)       gTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAgg                                                                                                                                              >  1:1146002/1‑150 (MQ=255)           cAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCAc                                                                                                                                          <  1:1077609/150‑1 (MQ=255)                ccgccTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCa                                                                                                                                     <  2:702484/150‑1 (MQ=255)                  gccTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGa                                                                                                                                   >  2:169145/1‑150 (MQ=255)                       cTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTc                                                                                                                              <  1:1211224/150‑1 (MQ=255)                         cGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGc                                                                                                                            <  1:1164142/150‑1 (MQ=255)                              gTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGTAGTCCACGCCCAGATGGTCGCCAAAg                                                                                                                       <  1:814029/150‑1 (MQ=255)                              gTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAg                                                                                                                       <  1:160013/150‑1 (MQ=255)                                   ttCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACg                                                                                                                  >  1:109386/1‑150 (MQ=255)                                   ttCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACg                                                                                                                  >  1:1149606/1‑150 (MQ=255)                                      gcgcATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAg                                                                                                               <  2:109386/150‑1 (MQ=255)                                           tGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCtt                                                                                                          >  1:756401/1‑150 (MQ=255)                                                tAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTg                                                                                                                                                   >  2:643182/1‑104 (MQ=255)                                                tAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTg                                                                                                                                                   <  1:643182/104‑1 (MQ=255)                                                 aGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCgct                                                                                                    >  2:1087365/1‑150 (MQ=255)                                                  gATCAGCGGCAGTGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTGCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCgctg                                                                                                   >  2:1013480/1‑150 (MQ=255)                                                  gATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCGCAGGGTTTGCGAGTCCCCCTGGTAGTCCAGCAGATCGACAACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGACTTCGCgctg                                                                                                   >  2:494250/1‑150 (MQ=255)                                                   aTCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCgctgc                                                                                                  <  2:683117/150‑1 (MQ=255)                                                   aTCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGCTCTGGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCgctgc                                                                                                  >  2:501244/1‑150 (MQ=255)                                                    tCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCgctgct                                                                                                 >  2:401385/1‑150 (MQ=255)                                                          gCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCgg                                                                                           <  1:1087365/150‑1 (MQ=255)                                                           cAGGGGAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGt                                                                                          >  1:1025922/1‑150 (MQ=255)                                                            aGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTg                                                                                         <  1:1058187/150‑1 (MQ=255)                                                            aGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTg                                                                                         <  1:169145/150‑1 (MQ=255)                                                                tAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCt                                                                                     >  1:1135184/1‑150 (MQ=255)                                                                  ggTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTgg                                                                                   <  2:1193788/150‑1 (MQ=255)                                                                   gTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTggg                                                                                  <  1:1013480/150‑1 (MQ=255)                                                                         ccTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAgcg                                                                            <  2:1140216/150‑1 (MQ=255)                                                                                       tcACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAgg                                                                                                                                              >  1:473011/1‑70 (MQ=255)                                                                                       tcACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAgg                                                                                                                                              <  2:473011/70‑1 (MQ=255)                                                                                           cGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACtgt                                                                <  1:366539/144‑1 (MQ=255)                                                                                           cGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACtgt                                                                >  2:366539/1‑144 (MQ=255)                                                                                             aCGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGaa                                                        <  1:1045464/150‑1 (MQ=255)                                                                                               gTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGc                                                      >  2:314058/1‑150 (MQ=255)                                                                                                 tCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCtt                                                    >  2:542610/1‑150 (MQ=255)                                                                                                       ccAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTc                                                   <  2:900927/145‑1 (MQ=255)                                                                                                       ccAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTc                                                   >  1:900927/1‑145 (MQ=255)                                                                                                       ccAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACa                                              >  1:155092/1‑150 (MQ=255)                                                                                                       ccAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACa                                              <  1:314058/150‑1 (MQ=255)                                                                                                       ccAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACa                                              <  2:155092/150‑1 (MQ=255)                                                                                                         aGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGc                                            <  2:1149606/150‑1 (MQ=255)                                                                                                         aGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGc                                            <  2:117586/150‑1 (MQ=255)                                                                                                             tCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCg                                        >  1:683692/1‑150 (MQ=255)                                                                                                               tGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGcc                                      >  1:823066/1‑150 (MQ=255)                                                                                                                  gAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTc                                   <  2:1135184/150‑1 (MQ=255)                                                                                                                   aGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCt                                  <  1:401385/150‑1 (MQ=255)                                                                                                                          cTTGTAGTCCAGCAGGTCGTCGACCAGCGGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCcgcg                           >  1:236109/1‑150 (MQ=255)                                                                                                                           tggccttCCAGCTGGTCGACGACCTGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGACCTGGGCCAGCGCCGCAGCACAGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCCGCGa                          >  1:219349/7‑150 (MQ=255)                                                                                                                                  ccAGCTGGTCGACGACCTGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCCGCGAATGACCt                   >  1:153763/1‑150 (MQ=255)                                                                                                                                  ccAGCTGGTCGACGACCTGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCCGCGAATGACCt                   >  1:746426/1‑150 (MQ=255)                                                                                                                                   cagctgGTCGACGACCTGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCCGCGAATTACCTc                  >  2:598855/1‑150 (MQ=255)                                                                                                                                   cagcagGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCCGCGAATGACCTc                  <  2:756401/150‑1 (MQ=255)                                                                                                                                     gctggTCGACGACCTGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTc                                                   >  2:365044/4‑115 (MQ=255)                                                                                                                                     gctggTCGACGACCTGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTc                                                   <  1:365044/112‑1 (MQ=255)                                                                                                                                            gtcgACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCCGCGAATGACCTCCATGTAGAc         >  1:1063077/1‑150 (MQ=255)                                                                                                                                            gtcgACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCCGCGAATGACCTCCATGTAGAc         >  2:1016549/1‑150 (MQ=255)                                                                                                                                            gtcgACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCCGCGAATGACCTCCATGTAGAc         >  1:1063185/1‑150 (MQ=255)                                                                                                                                             tcgACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCCGCGAATGACCTCCATGTAGACt        >  1:311436/1‑150 (MQ=255)                                                                                                                                                  cAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCCGCGAATGACCTCCATg              >  2:445684/1‑139 (MQ=255)                                                                                                                                                  cAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCCGCGAATGACCTCCATg              <  1:445684/139‑1 (MQ=255)                                                                                                                                                  cAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCCGCGAATGACCTCCATGTAGACTTCCTc   >  1:1150094/1‑150 (MQ=255)                                                                                                                                                  cAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCCGCGAATGACCTCCATGTAGACTTCCTc   <  2:823066/150‑1 (MQ=255)                                                                                                                                                   aGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCCGCGAATGACCTCCATGTAGACTTCCTCg  >  1:933404/1‑150 (MQ=255)                                                                                                                                                   aGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCCGCGAATGACCTCCATGTAGACTTCCTCg  <  2:311436/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                      CCTGGCGTACCAGCGCCGCCTGCTCGGCAGTGCCTTCGCGCATGGTGTAGATCAGCGGCAGGGTAGGTTTGCCCTCGGCCAGGTCGTCACCGACGTTCTTGCCCAGGGTCTGCGAGTCACCCTTGTAGTCCAGCAGGTCGTCGACCAGCTGGAAGGCCACGCCCAGATGGTCGCCAAAGGTACGCAGGGCTTCGCGCTGCTCGTCGGTGGCCTGGGCCAGCGCCGCAGCACTGTGGGTAGAAGCTTCGAACAGCATCGCCGTCTTGCCGCGAATGACCTCCATGTAGACTTCCTCG  >  NC_002947/799704‑799999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑