| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 913,440 | G→T | 10.0% | A50S (GCC→TCC) | fruA → | PTS fructose transporter subunit IIBC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 913,440 | 0 | G | T | 10.0% | 151.8 / 2.7 | 49 | A50S (GCC→TCC) | fruA | PTS fructose transporter subunit IIBC |
| Reads supporting (aligned to +/- strand): ref base G (21/23); new base T (2/3); total (23/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
AACATTGCCATAGTCACCGCCTGCCCCAATGGCCAGGTATCGAGTGTACTGAGTGCACGCTTGCTGTCCGCCGCCGCCCAGCGTCGCGGCTGGAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCCCGCTTCGTCGGCAAGCGCCTGTACCAAAGCACACCGTCCCAGGCGCTGGCTGATCGCGAAGGCTTTCTCGACGAAGCGGCGG > NC_002947/913296‑913584 | aaCATTGCCATAGTCACCGCCTGCCCCAATGGCCAGGTATCGAGTGTACTGAGTGCACGCTTGCTGTCCGCCGCCGCCCAGCGTCGCGGCTGGAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGca < 1:1085735/150‑1 (MQ=255) gCCATAGTCACCGCCTGCCCCAATGGCCAGGTATCGAGTGTACTGAGTGCACGCTTGCTGTCCGCCGCCGCCCAGCGTCGCGGCTGGAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATc < 2:493033/150‑1 (MQ=255) tCACCGCCTGCCCCAATGGCCAGGTATCGAGTGTACTGAGTGCACGCTTGCTGTCCGCCGCCGCCCAGCGTCGCGGCTGGAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAgg > 2:160662/1‑150 (MQ=255) ccAATGGCCAGGTATCGAGTGTACTGAGTGCACGCTTGCTGTCCGCCGCCGCCCAGCGTCGCGGCTGGAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGc > 1:61072/1‑150 (MQ=255) ccAATGGCCAGGTATCGAGTGTACTGAGTGCACGCTTGCTGTCCGCCGCCGCCCAGCGTCGCGGCTGGAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGc > 2:76631/1‑150 (MQ=255) ggTATCGAGTGTACTGAGTGCACGCTTGCTGTCCGCCGCCGCCCAGCGTCGCGGCTGGAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAg > 1:353016/1‑150 (MQ=255) gtgtACTGAGTGCACGCTTGCTGTCCGCCGCCGCCCAGCGTCGCGGCTGGAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGc < 1:717991/150‑1 (MQ=255) tGAGTGCACGCTTGCTGTCCGCCGCCGCCCAGCGTCGCGGCTGGAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCtt > 1:827897/1‑128 (MQ=255) tGAGTGCACGCTTGCTGTCCGCCGCCGCCCAGCGTCGCGGCTGGAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCtt < 2:827897/128‑1 (MQ=255) gTGCACGCTTGCTGTCCGCCGCCGCCCAGCGTCGCGGCTGGAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGAcc > 1:252181/1‑150 (MQ=255) gCACGCTTGCTGTCCGCCGCCGCCCAGCGTCGCGGCTGGAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTg < 1:1180115/150‑1 (MQ=255) cACGCTTGCTGTCCGCCGCCGCCCAGCGTCGCGGCTGGAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGcc > 2:865460/1‑104 (MQ=255) cACGCTTGCTGTCCGCCGCCGCCCAGCGTCGCGGCTGGAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGcc < 1:865460/104‑1 (MQ=255) cACGCTTGCTGTCCGCCGCCGCCCAGCGTCGCGGCTGGAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTgg < 2:252181/150‑1 (MQ=255) aCGCTTGCTGTCCGCCGCCGCCCAGCGTCGCGGCTGGAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGa > 2:1025836/1‑144 (MQ=255) aCGCTTGCTGTCCGCCGCCGCCCAGCGTCGCGGCTGGAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGa < 1:1025836/144‑1 (MQ=255) ttGCTGTCCGCCGCCGCCCAGCGTCGCGGCTGGAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCCCGc > 1:353719/1‑150 (MQ=255) tGTCCGCCGCCGCCCAGCGTCGCGGCTGGAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCCCGCTtcg > 2:174512/1‑150 (MQ=255) gTCCGCCGCCGCCCAGCGTCGCGGCTGGAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCCCGCTtcgt < 1:160662/150‑1 (MQ=255) ccgccgccgCCCAGCGTCGCGGCTGGAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCCCGCTtcgtcg < 2:343686/150‑1 (MQ=255) gccgccCAGCGTCGCGGCTGGAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGAc > 2:243529/1‑129 (MQ=255) gccgccCAGCGTCGCGGCTGGAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGAc < 1:243529/129‑1 (MQ=255) ccgccCAGCGTCGCGGCTGGAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACATGGCCCGCTTCGTCGGCAAgc < 2:61072/150‑1 (MQ=255) cccAGCGTCGCGGCTGGAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTtgtg < 2:716909/104‑1 (MQ=255) cccAGCGTCGCGGCTGGAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTtgtg > 1:716909/1‑104 (MQ=255) ccAGCGTCGCGGCTGGAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCCCGCTTCGTCGGCAAGCGCCt < 2:1000874/150‑1 (MQ=255) ccAGCGTCGCGGCTGGAGCACCAACGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCCCGCTTCGTCGGCAAGCGCCt > 2:1009627/1‑150 (MQ=255) gcgGCTGGAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCCCGCTTCGTCGGCAAGCGCCTGTACCaaa < 2:353719/150‑1 (MQ=255) ggAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCCCGCTTCGTCGGCAAGCGCCTGTACCAAAGCacac < 2:725880/150‑1 (MQ=255) cAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGa < 1:779368/63‑1 (MQ=255) cAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGa > 2:779368/1‑63 (MQ=255) gCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCCCGCTTCGTCGGCAAGCGCCTGTCCCAAACCACACCGCCCCAgg > 2:649548/1‑150 (MQ=255) ttGAACAACTCGGGCTGCAACTGCGCTGCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCCCGCTTCGTCGGCAAGCGCCTGTACCAAAGCACACCGTCCCAGGCGCTGGCTGATCGCGAAg < 2:595648/149‑1 (MQ=255) tGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCCCGCTTCGTCGGCAAGCGCCTGTACCAAAGCACACCGTCCCAGGCGCTGGCTGATCGCGAAgg > 2:642840/1‑150 (MQ=255) tGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCCCGCTTCGTCGGCAAGCGCCTGTACCAAAGCACACCGTCCCAGGCGCTGGCTGATCGCGAAgg > 1:595648/1‑150 (MQ=255) aaCACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCCCGCTTCGTCGGCAAGCGCCTGTACCAAAGCACACCGTCCCAGGCGCTGGCTGATCGCGaa < 2:979505/146‑1 (MQ=255) aaCACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCCCGCTTCGTCGGCAAGCGCCTGTACCAAAGCACACCGTCCCAGGCGCTGGCTGATCGCGaa > 1:979505/1‑146 (MQ=255) cacCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCCCGCTTCGTCGGCAAGCGCCTGTACCAAAGCACACCGTCCCAGGCGCTGGCTGATCGCGAAGGCttt < 2:353016/150‑1 (MQ=255) cGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCCCGCTTCGTCGGCAAGCGCCTGTACCAAAGCACACCGTCCCAGGCGCTGGCTGATCGCGAAGGCTTTCTcga < 2:1166538/150‑1 (MQ=255) cGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCACGCTTCGTCGGCAAGCGCCTGTACCAAAGCACACCGTCCCAGGCGCTTGCTGATCGCTAAGGCTTTCTcga < 2:211893/150‑1 (MQ=255) gCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCCCGCTTCGTCGGCAAGCGCCTGTACCAAAGCACACCGTCCCAGGCGCTGGCTGATCGCGAAGGCTTTCTCGACGAAgc > 1:380995/1‑150 (MQ=255) tGAGCTCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCCCGCTTCGTCGGCAAGCGCCTGTACCAAAGCACACCGTccc > 2:289960/1‑108 (MQ=255) tGAGCTCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCCCGCTTCGTCGGCAAGCGCCTGTACCAAAGCACACCGTccc > 2:289929/1‑108 (MQ=255) tGAGCTCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCCCGCTTCGTCGGCAAGCGCCTGTACCAAAGCACACCGTccc < 1:289960/108‑1 (MQ=255) tGAGCTCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCCCGCTTCGTCGGCAAGCGCCTGTACCAAAGCACACCGTccc < 1:289929/108‑1 (MQ=255) tGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCCCGCTTCGTCGGCAAGCGCCTGTACCAAAGCACACCGTCCCAGGCGCTGGCTGATCGCGGAGGCTTTCTCGACGAAGcggcgg < 1:174512/150‑1 (MQ=255) tGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCCCGCTTCGTCGGCAAGCGCCTGTACCAAAGCACACCGTCCCAGGCGCTGGCTGATCGCGAAGGCTTTCTCGACGAAGcggcgg > 2:697262/1‑150 (MQ=255) aGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCCCGCTTCGTCGGCAAGCGCCTGTACCAAAGCACAcc > 2:467379/1‑101 (MQ=255) aGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCCCGCTTCGTCGGCAAGCGCCTGTACCAAAGCACAcc < 1:467379/101‑1 (MQ=255) gccgcACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCCCGCTTCGTCGGCAAGCGCCTGTACCAAAGCACACCGTCCCAGGCGCTGGCTGATCGCGAAGGCTTTCTCGACGAAgcggcg > 2:887626/1‑144 (MQ=255) gccgcACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCCCGCTTCGTCGGCAAGCGCCTGTACCAAAGCACACCGTCCCAGGCGCTGGCTGATCGCGAAGGCTTTCTCGACGAAgcggat < 1:887626/144‑3 (MQ=255) | AACATTGCCATAGTCACCGCCTGCCCCAATGGCCAGGTATCGAGTGTACTGAGTGCACGCTTGCTGTCCGCCGCCGCCCAGCGTCGCGGCTGGAGCACCAGCGTCGAAGTGCAGAATACTGAACACCCCGAGCGGCAACTGAGCGCCGCACAGATCGCCGAGGCTGACTGGGTGCTTGTGGTCAGCACCGGGCCGGTGGACCTGGCCCGCTTCGTCGGCAAGCGCCTGTACCAAAGCACACCGTCCCAGGCGCTGGCTGATCGCGAAGGCTTTCTCGACGAAGCGGCGG > NC_002947/913296‑913584 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |