Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 946,922 C→A 8.0% Q150H (CAG→CAT)  norR ← DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947946,9220CA 8.0% 154.5 / 3.2 50Q150H (CAG→CAT) norRDNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (21/25);  new base A (2/2);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

GGGCTCTGGCCAATCAGCTCTCTATCCTGGCCGCTGGCCTGGCGGTAGAGCTCGGCGCGATGGTGTTCGTCTTCGGCGCGCAGGGCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGCCCCATGGGCGCTCATCGACCATCAACGGGCAGCCCATGCAGTCGTGCACTTCAAGGTCGGCATCGGGGGCATTGACCAAAC  >  NC_002947/946779‑947067
                                                                                                                                               |                                                                                                                                                 
gggCTCTGGCCAATCAGCTCTCTATCCTGGCCGCTGGCCTGGCGGTAGAGCTCGGCGCGATGGTGTTCGTCTTCGGCGCGCAGGGCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAgg                                                                                                                                             <  2:141521/150‑1 (MQ=255)
   ctctGGCCAATCAGCTCTCTATCCTGGCCGCTGGCCTGGCGGTAGAGCTCGGCGCGATGGTGTTCGTCTTCGGCGCGCAGGGCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCa                                                                                                                                               <  2:381954/145‑1 (MQ=255)
   ctctGGCCAATCAGCTCTCTATCCTGGCCGCTGGCCTGGCGGTAGAGCTCGGCGCGATGGTGTTCGTCTTCGGCGCGCAGGGCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCa                                                                                                                                               >  1:381954/1‑145 (MQ=255)
   ctctGGCCAATCAGCTCTCTATCCTGGCCGCTGGCCTGGCGGTAGAGCTCGGCGCGATGGTGTTCGTCTTCGGCGCGCAGGGCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCa                                                                                                                                          >  1:190523/1‑150 (MQ=255)
   ctctGGCCAATCAGCTCTCTATCCTGGCCGCTGGCCTGGCGGTAGAGCTCGGCGCGATGGTGTTCGTCTTCGGCGCGCAGGGCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCa                                                                                                                                          <  2:1201555/150‑1 (MQ=255)
     ctGGCCAATCAGCTCTCTATCCTGGCCGCTGGCCTGGCGGTAGAGCTCGGCGCGATGGTGTTCGTCTTCGGCGCGCAGGGCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATc                                                                                                                                        <  2:904372/150‑1 (MQ=255)
     ctGGCCAATCAGCTCTCTATCCTGGCCGCTGGCCTGGCGGTAGAGCTCGGCGCGATGGTGTTCGTCTTCGGCGCGCAGGGCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATc                                                                                                                                        <  2:253020/150‑1 (MQ=255)
      tGGCCAATCAGCTCTCTATCCTGGCCGCTGGCCTGGCGGTAGAGCTCGGCGCGATGGTGTTCGTCTTCGGCGCGCAGGGCCAGGTGCTCGATACGCTCGGCCACGGAAAAGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATcc                                                                                                                                       >  1:851272/1‑150 (MQ=255)
           aaTCAGCTCTCTATCCTGGCCGCTGGCCTGGCGGTAGAGCTCGGCGCGATGGTGTTCGTCTTCGGCGCGCAGGGCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATCCAGCTc                                                                                                                                  >  2:709170/1‑150 (MQ=255)
             tCAGCTCTCTATCCTGGCCGCTGGCCTGGCGGTAGAGCTCGGCGCGATGGTGTTCGTCTTCGGCGCGCAGGGCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGTAGGGCATCCAGCTCCa                                                                                                                                <  1:955886/150‑1 (MQ=255)
                      tATCCTGGCCGCTGGCCTGGCGGTAGAGCTCGGCGCGATGGTGTTCGTCTTCGGCGCGCAGGGCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGa                                                                                                                       <  1:566609/150‑1 (MQ=255)
                       aTCCTGGCCGCTGGCCTGGCGGTAGAGCTCGGCGCGATGGTGTTCGTCTTCGGCGCGCAGGGCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGaa                                                                                                                      <  1:609349/150‑1 (MQ=255)
                             gCCGCTGGCCTGGCGGTAGAGCTCGGCGCGATGGTGTTCGTCTTCGGCGCGCAGGGCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAg                                                                                                                                              >  2:858725/1‑120 (MQ=255)
                             gCCGCTGGCCTGGCGGTAGAGCTCGGCGCGATGGTGTTCGTCTTCGGCGCGCAGGGCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAg                                                                                                                                              <  1:858725/120‑1 (MQ=255)
                               cGCTGGCCTGGCGGTAGAGCTCGGCGCGATGGTGTTCGTCTTCGGCGCGCAGGGCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATc                                                                                                                                        >  1:1045917/1‑124 (MQ=255)
                               cGCTGGCCTGGCGGTAGAGCTCGGCGCGATGGTGTTCGTCTTCGGCGCGCAGGGCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATc                                                                                                                                        <  2:1045917/124‑1 (MQ=255)
                                      ctggcGGTAGAGCTCGGCGCGATGGTGTTCGTCTTCGGCGCGCAGGGCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGTCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAg                                                                                                       <  2:467514/150‑1 (MQ=255)
                                                 gCTCGCCTCGATGGTGTTCGTCATAGCAGCGCAAGGACAGGTGCTTGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAggg                                                                                            <  2:851272/150‑1 (MQ=255)
                                                   tCGGCGCGATGGTGTTCGTCTTCGGCGCGCAGGGCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTg                                                                                          >  2:1070460/1‑150 (MQ=255)
                                                    cGGCGCGATGGTGTTCGTCTTCGGCGCGCAGGGCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGa                                                                                         <  1:1070460/150‑1 (MQ=255)
                                                      gcgcGATGGTGTTCGTCTTCGGCGCGCAGGGCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGAcc                                                                                       >  2:556962/1‑150 (MQ=255)
                                                      gcgcGATGGTGTTCGTCTTCGGCGCGCAGGGCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGAcc                                                                                       <  2:723442/150‑1 (MQ=255)
                                                       cgcgATGGTGTTCGTCTTCGGCGCGCAGGGCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCa                                                                                      <  2:515099/150‑1 (MQ=255)
                                                          gATGGTGTTCGTCTTCGGCGCGCAGGGCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGc                                                                                   <  2:592030/150‑1 (MQ=255)
                                                          gATGGTGTTCGTCTTCGGCGCGCAGGGCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGc                                                                                   >  2:1001749/1‑150 (MQ=255)
                                                            tGGTGTTCGTCTTCGGCGCGCAGGGCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGAGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGccc                                                                                 <  1:913426/150‑1 (MQ=255)
                                                                    gTCTTCGGCGCGCAGGGCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGCCCCATGGgcg                                                                         <  2:190523/150‑1 (MQ=255)
                                                                     tCTTCGGCGCGCAGGGCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGCCCCATGGgcgc                                                                        >  2:1054089/1‑150 (MQ=255)
                                                                              cgcAGGGCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGCCCCATGGGCGCTCATCGAcc                                                               <  1:556962/150‑1 (MQ=255)
                                                                                   ggCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGCCCCATGGGCGCTCATCGACCATCaa                                                          >  1:538721/1‑150 (MQ=255)
                                                                                   ggCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCATGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGCCCCATGGGCGCTCATCGACCATCaa                                                          >  2:1189936/1‑150 (MQ=255)
                                                                                   ggCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCATGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGCCCCATGGGCGCTCATCGACCATCaa                                                          >  2:1190007/1‑150 (MQ=255)
                                                                                          tGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGCCCCATGGGCGCTCATCGACCATCAACGGGCAg                                                   <  1:709170/150‑1 (MQ=255)
                                                                                               gATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGCCCCATGGGCGCTCATCGACCATCAACGGGCAGCCCAt                                              >  2:1081594/1‑150 (MQ=255)
                                                                                                aTACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGCCCCATGGGCGCTCATCGACCATCAACGGGCAGCCCATg                                             >  1:1183971/1‑150 (MQ=255)
                                                                                                aTACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGCCCCATGGGCGCTCATCGACCATCAACGGGCAGCCCATg                                             >  1:500927/1‑150 (MQ=255)
                                                                                                    gCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGCCCCATGGGCGCTCATCGACCATCAACGGGCAGCCCATGCAGt                                         >  1:644072/1‑150 (MQ=255)
                                                                                                     cTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGCCCCATGGGCGCTCATCGACCATCAACGGGCAGCCCATGCAGTc                                        <  1:1001749/150‑1 (MQ=255)
                                                                                                      tCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGACCGGGGGTGAGCGCCTCGAGGCTGACCAGGCCCCATGGGCGCTCATCGACCATCAACGGTCAGCCCATGCAGTCg                                       >  2:1067698/1‑150 (MQ=255)
                                                                                                       cGGCCACGGTAACGGTGGCGGCAGCCAGGCGGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGCCCCATGGGCGCTCATCGACCATCAACGGGCAGCCCATGCAGTCGt                                      <  2:365527/150‑1 (MQ=255)
                                                                                                          ccACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGCCCCATGGGCGCTCATCGACCATCAACGGGCAGCCCATGCAGTCGTGCa                                   >  1:842473/1‑150 (MQ=255)
                                                                                                                     gTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGCCCCATGGGCGCTCATCGACCATCAACGGGCAGCCCATGCAGTCGTGCACTTCAAGGTCg                        <  1:1054089/150‑1 (MQ=255)
                                                                                                                      tGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGCCCCATGGGCGCTCATCGACCATCAACGGGCAGCCCATGCAGTCGTGCACTTCAAGGTCgg                       >  2:1248465/1‑150 (MQ=255)
                                                                                                                            cAGCCAGGCTGGCAAAGGCATGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGCCCCATGGGCGCTCATCGACCATCAACGGGCAGCCCATGCAGTCGTGCACTTCAAGGTCGGCATCgg                 <  1:1190007/150‑1 (MQ=255)
                                                                                                                            cAGCCAGGCTGGCAAAGGCATGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGCCCCATGGGCGCTCATCGACCATCAACGGGCAGCCCATGCAGTCGTGCACTTCAAGGTCGGCATCgg                 <  1:1189936/150‑1 (MQ=255)
                                                                                                                                  ggCTGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGCCCCATGGGCGCTCATCGACCATCAACGGGCAGCCCATGCAGTCGTGCACTTCAAGGTCGGCATCGGGGGCAt           <  2:644072/150‑1 (MQ=255)
                                                                                                                                     tGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGCCCCATGGGCGCTCATCGACCATCAACGGGCAGCCCATGCAGTCGTGCACTTCAAGGTCGGCATCGGGGGCATTGa        >  1:638573/1‑150 (MQ=255)
                                                                                                                                      ggCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGCCCCATgg                                                                            >  2:696861/1‑81 (MQ=255)
                                                                                                                                      ggCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGCCCCATgg                                                                            <  1:696861/81‑1 (MQ=255)
                                                                                                                                           aGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGCCCCATGGGCGCTCATCGACCATCAATGGGCAGCCCATGCAGTCGTGCACTTCAAGGTCGGCATCGGGGGCATTGACCAAAc  >  2:64925/1‑150 (MQ=255)
                                                                                                                                               cTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGCCCCATGGGCGCTCATCGACCATCAACGGGCAGCCCATGCAGTCGTGCACTTCAAGGTCGGCATCGGGGGCATTGAc       <  1:476371/141‑1 (MQ=255)
                                                                                                                                               cTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGCCCCATGGGCGCTCATCGACCATCAACGGGCAGCCCATGCAGTCGTGCACTTCAAGGTCGCCATCGGGGGCATTGAc       >  2:476371/1‑141 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
GGGCTCTGGCCAATCAGCTCTCTATCCTGGCCGCTGGCCTGGCGGTAGAGCTCGGCGCGATGGTGTTCGTCTTCGGCGCGCAGGGCCAGGTGCTCGATACGCTCGGCCACGGTAACGGTGGCGGCAGCCAGGCTGGCAAAGGCCTGCAGGGCATCCAGCTCCAGGCTCTGGAACTGCCCGGGGGTGAGGGCGTCGAGGGTGACCAGGCCCCATGGGCGCTCATCGACCATCAACGGGCAGCCCATGCAGTCGTGCACTTCAAGGTCGGCATCGGGGGCATTGACCAAAC  >  NC_002947/946779‑947067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑