Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 971,680 C→T 9.7% intergenic (+32/‑32) yajC → / → secD protein translocase subunit YajC/protein translocase subunit SecD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947971,6800CT 9.7% 108.7 / 3.1 41intergenic (+32/‑32)yajC/secDprotein translocase subunit YajC/protein translocase subunit SecD
Reads supporting (aligned to +/- strand):  ref base C (18/19);  new base T (2/2);  total (20/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

TCAAGGTTTCCGATGACTTCGTGGTGCTGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACCTCTACCCGGATGACCCGGCGGTACAGATCAGCGGT  >  NC_002947/971536‑971828
                                                                                                                                                |                                                                                                                                                    
tCAAGGTTTCCGATGACTTCGTGGTGCTGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAAcgc                                                                                                                                                 <  2:738869/150‑1 (MQ=255)
     gTTTCCGATGACTTCGTGGTGCTGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGCCGGGGCGCGCACCGCGcccc                                                                                                                                            <  2:983272/150‑1 (MQ=255)
        tCCGATGACTTCGTGGTGCTGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGTTGCGCGCCCcgac                                                                                                                                         <  2:1260503/150‑3 (MQ=255)
         ccGATGACTTCGTGGTGCTGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGt                                                                                                                                        <  1:610044/150‑1 (MQ=255)
          gatctaCTTCGTGGTGCTGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTAGGGGCGCGCAACGCGCCCCGCGTc                                                                                                                                       <  2:542951/145‑1 (MQ=255)
                cTTCGTGGTGCTGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAAc                                                                                                                                 <  1:571395/150‑1 (MQ=255)
                cTTCGTGGTGCTGGAAGTGTCCGACACTGTCGAGCAAAAATACCACCCGTAAATCATTGCTGCGACCCTGCTAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTAGGGGCGCGCAACGCGCCCCGCGTCtagatc                                                                                                                                 >  1:542951/1‑145 (MQ=255)
                 ttCGTGGTGCTGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCATGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACg                                                                                                                                >  2:712809/1‑150 (MQ=255)
                           tGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGa                                                                                                                      <  1:885550/150‑1 (MQ=255)
                                gtgtCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTg                                                                                                                 <  2:775762/150‑1 (MQ=255)
                                                    gAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGggcggc                                                                                                                          <  2:672300/121‑1 (MQ=255)
                                                    gAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGggcggc                                                                                                                          >  1:672300/1‑121 (MQ=255)
                                                     aaGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGaaa                                                                                            >  1:1183990/1‑150 (MQ=255)
                                                       gTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAata                                                                                          >  2:604353/1‑150 (MQ=255)
                                                        ttCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAatat                                                                                         >  1:197277/1‑150 (MQ=255)
                                                                gggTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAatat                                                                                         >  1:1245503/1‑142 (MQ=255)
                                                                gggTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAatat                                                                                         <  2:1245503/142‑1 (MQ=255)
                                                                       aTTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGGCGGGGCGCGTTGCGCGCCCcgact                                                                                                                                        >  2:721106/1‑85 (MQ=37)
                                                                       aTTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGGCGGGGCGCGTTGCGCGCCCcgact                                                                                                                                        <  1:721106/88‑4 (MQ=37)
                                                                          gctgcGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTa                                                                       >  1:1063396/1‑150 (MQ=255)
                                                                            tgcGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACt                                                                     >  2:478352/1‑150 (MQ=255)
                                                                              cGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACtgg                                                                   <  2:197277/150‑1 (MQ=255)
                                                                                 cccTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCACGCACCTTGAACGGGCGGCGTGATGATGAAAAAAGATCTGCTGTGGAAATATGCACTGATCGTGCTGGTACtggtgg                                                                >  1:726013/1‑150 (MQ=255)
                                                                                      ccAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGt                                                           <  2:1063396/150‑1 (MQ=255)
                                                                                                 gCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTc                                                <  2:1183990/150‑1 (MQ=255)
                                                                                                       ggCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTcc                                          >  1:994675/1‑150 (MQ=255)
                                                                                                                aGTTACCGGTTTTATTTTCCAGGCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACCTCTa                                 <  2:726013/150‑1 (MQ=255)
                                                                                                                     ccGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCct                                                                                                     <  2:1220182/77‑1 (MQ=255)
                                                                                                                     ccGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCct                                                                                                     >  1:1220182/1‑77 (MQ=255)
                                                                                                                      cGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAat                                                                                                          <  2:1185103/71‑1 (MQ=255)
                                                                                                                      cGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAat                                                                                                          >  1:1185103/1‑71 (MQ=255)
                                                                                                                       ggTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACCTCTACCCGGAt                          <  1:712809/150‑1 (MQ=255)
                                                                                                                         ttttATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACGAATATCCTCTGTGGAAATATGCa                                                                                      >  1:731327/1‑88 (MQ=255)
                                                                                                                         ttttATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACGAATATCCTCTGTGGAAATATGCa                                                                                      <  2:731327/88‑1 (MQ=255)
                                                                                                                           ttATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACCTCTACCCGGATGAcc                      >  1:500568/1‑150 (MQ=255)
                                                                                                                                    aGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACCTCTACCCGGATGACCCGGCGGTAc             >  2:1088680/1‑150 (MQ=255)
                                                                                                                                     cgcGGGGCGCGTTGCGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACCTCTACCCGGATGACCCGGCGGTACa            >  1:352418/3‑150 (MQ=255)
                                                                                                                                        ggggCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAAcc                                     <  1:81108/122‑1 (MQ=255)
                                                                                                                                        ggggCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAAcc                                     >  2:81108/1‑122 (MQ=255)
                                                                                                                                         gggCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCtgt                                                                                                 <  1:1038867/61‑1 (MQ=255)
                                                                                                                                         gggCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCtgt                                                                                                 >  2:1038867/1‑61 (MQ=255)
                                                                                                                                               gcAACCCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCGTCTGTGGAAATATGCACTGATCGTGCTCCTACTGGTGGTGGGGGTCATTTATTCCGCTCCCAACGCCTACCCGGATGACCCGGCGGTACAGATCAGCGGt  <  1:478352/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TCAAGGTTTCCGATGACTTCGTGGTGCTGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACCTCTACCCGGATGACCCGGCGGTACAGATCAGCGGT  >  NC_002947/971536‑971828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑