| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 977,876 | T→A | 8.0% | intergenic (+39/‑141) | cysE → / → iscR | serine acetyltransferase/DNA‑binding transcriptional regulator IscR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 977,876 | 0 | T | A | 8.0% | 145.5 / 3.6 | 50 | intergenic (+39/‑141) | cysE/iscR | serine acetyltransferase/DNA‑binding transcriptional regulator IscR |
| Reads supporting (aligned to +/- strand): ref base T (23/23); new base A (2/2); total (25/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
CGCTGACCAAGATGGGCAGTGACTATTGTGCCAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAG‑TAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGCATACTCGCACACATCCTGAAACTCCCGTGGTACCCAATAGCCATGCGACTG > NC_002947/977728‑978025 | cccggACCAAGATGGACAGTGACTATTGTGCCAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGtgtg < 1:857760/146‑1 (MQ=255) gcTGACCAAGATGGGCAGTGACTATTGTGCCAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGc < 2:331918/150‑1 (MQ=255) tGACCAAGATGGGCAGTGACTATTGTGCCAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGAGcac > 1:696016/1‑148 (MQ=255) aCCAAGATGGGCAGTGACTATTGTGCCAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCAc > 1:508176/1‑150 (MQ=255) cAAGATGGGCAGTGACTATTGTGCCAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCaagg > 2:824266/1‑147 (MQ=255) cAAGATGGGCAGTGACTATTGTGCCAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGc > 2:533966/1‑150 (MQ=255) cAAGATGGGCAGTGACTATTGTGCCAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGc < 2:148787/150‑1 (MQ=255) gATGGGCAGTGACTATTGTGCCAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGcccc > 1:1065013/1‑150 (MQ=255) ggCAGTGACTATTGTGCCAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGAGCACACGccccgcat < 2:696016/150‑5 (MQ=255) ggCAGTGACTATTGTGCCAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGAGGGGCGTGAGCACACGccccgcat < 2:936881/150‑5 (MQ=255) gTGACTATTGTGCCAAGGAACGGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGc < 1:718587/150‑1 (MQ=255) aTTGTGCCAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAAt > 2:84563/1‑150 (MQ=255) tgCCAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGc > 2:59817/1‑123 (MQ=255) tgCCAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGc < 1:59817/123‑1 (MQ=255) ccAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGcgcc > 2:739642/1‑150 (MQ=255) cAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGcgccc < 2:149722/150‑1 (MQ=255) cAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGcgccc < 2:809768/150‑1 (MQ=255) aaGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGtgtg > 1:470345/1‑118 (MQ=255) aaGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGtgtg < 2:470345/118‑1 (MQ=255) ccGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGa < 1:739642/150‑1 (MQ=255) ccGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACCACACTTCTGAAGGCATGTGGGGCGTTTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGa > 1:152068/1‑150 (MQ=255) cGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGAt < 1:767748/150‑1 (MQ=255) ggaggaCGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCt < 2:445732/109‑1 (MQ=255) ggaggaCGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCt > 1:445732/1‑109 (MQ=255) ggaggaCGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGa < 2:508176/150‑1 (MQ=255) gaggaCGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGAc < 1:533966/150‑1 (MQ=255) gacTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGGTCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATc < 2:823348/116‑1 (MQ=255) gacTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATc > 1:823348/1‑116 (MQ=255) gAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGc > 2:154613/1‑89 (MQ=255) gAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGc < 1:154613/89‑1 (MQ=255) cccAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAG‑TAGGTCTAATAGtt > 1:658156/1‑150 (MQ=255) ccAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGAt > 1:957353/1‑116 (MQ=255) ccAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGAt < 2:957353/116‑1 (MQ=255) cAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAG‑TAGGTCTAATAGTTGa < 1:84563/150‑1 (MQ=255) ttactcTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAG‑TAGGTCTAATAGTTGACTTAAATGCTCggg < 2:1235310/145‑1 (MQ=255) ccGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAG‑TAGGTCTaa < 2:1174928/119‑1 (MQ=255) ccGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAG‑TAGGTCTaa > 1:1174928/1‑119 (MQ=255) cATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAG‑TAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGc < 2:688782/143‑1 (MQ=255) cATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAG‑TAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGc > 1:688782/1‑143 (MQ=255) aTTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAAt < 1:418097/69‑1 (MQ=255) aTTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAAt > 2:418097/1‑69 (MQ=255) ttGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAG‑TAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGCATACTCGca < 2:1065013/150‑1 (MQ=255) tCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAG‑TAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGCa < 2:409324/138‑1 (MQ=255) tCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAG‑TAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGCa > 1:409324/1‑138 (MQ=255) tCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAG‑TAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGCATACTCGcacaca > 2:975532/1‑150 (MQ=255) gccgcACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTTCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAG‑TAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGCATACTCGCACACATCCt > 1:669814/1‑150 (MQ=255) cgcACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAG‑TAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGCATACTCGCACACATCCTGa < 2:152068/150‑1 (MQ=255) gcACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAG‑TAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGCATACTCGCACACATCCTGaa > 2:617814/1‑150 (MQ=255) tGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAG‑TAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGCATACTCGCACACATCCTGAAACTc > 2:453300/1‑150 (MQ=255) tCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAG‑TAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGCATACTCGCACACATCCTGAAACTCCCGTg > 1:533274/1‑150 (MQ=255) aTGGTGGCATGCGGGGCGTGTGCTCACGCCCATCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCACGGCTACACCAGATTGATTCCAAGGATGA‑ACAAACGCGCGGGAATCGCATACTCGCACACATCCTGAAACTCCCGTGGt > 1:151421/1‑150 (MQ=255) gacgaggggCGTGAGCACACGCCCCGCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAG‑TAGGTCTAATAGTTGACTTAAATGATCGGGACTCGAATAAAGGCAAACAGCCGCAAACGCCCGTGGTACCCAAt > 2:781364/6‑150 (MQ=255) tGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAG‑TAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGCATACTCGCACACATCCTGAAACTCCCGTGGTACCCAATAg < 1:453300/150‑1 (MQ=255) tgtgCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAG‑TAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGCATACTCGCACACATCCTGAAACTCCCGTGGTACCCAATAGCCATGCgac > 1:51643/1‑150 (MQ=255) tgtgCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAG‑TAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGCATACTCGCACACATCCTGAAACTCCCGTGGTACCCAATAGCCATGCga > 2:1099656/1‑149 (MQ=255) tgtgCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAG‑TAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGCATACTCGCACACATCCTGAAACTCCCGTGGTACCCAATAGCCATGCga < 1:1099656/149‑1 (MQ=255) tgCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAG‑TAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGCATACTCGCACACATCCTGAAACTCCCGTGGTACCCAATAGCCATGCgactg < 2:51643/150‑1 (MQ=255) | CGCTGACCAAGATGGGCAGTGACTATTGTGCCAAGGAACTGCCGGCCTTGCCGGAGGACGACTTCAGCGAGGCTGCCCAAGTGGCCCAGCGCGACACTCAGCCGCATTGATCGCGCCGCACTGAAGTCATGGTGGCATGCGGGGCGTGTGCTCACGCCCCTCGTCTGCTACAATCGCGCCCGCCTCCCTGATGCAATCCCGACTAAAGCAG‑TAGGTCTAATAGTTGACTTAAATGCTCGGGAATCGCATACTCGCACACATCCTGAAACTCCCGTGGTACCCAATAGCCATGCGACTG > NC_002947/977728‑978025 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |