Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	181,943	C→T	23.4%	noncoding (52/120 nt)	PP_5SB →	5S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	181,943	0	C	T	23.4%	136.0 / 42.9	81	noncoding (52/120 nt)	PP_5SB	5S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (33/29);  new base T (12/7);  total (45/36)
Fisher's exact test for biased strand distribution p-value = 5.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

CTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCT  >  NC_002947/181802‑182090                                                                                                                                              |                                                                                                                                                     cTATCTGCACATGCAGGTAGGGGCTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAAc                                                                                                                                              >  2:536305/1‑150 (MQ=255)   aTCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGa                                                                                                                                            <  1:650945/150‑1 (MQ=255)         acaTGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATc                                                                                                                                      >  1:892893/1‑150 (MQ=255)                gatAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATg                                                                                                                               <  1:686604/148‑1 (MQ=255)                 gTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATgg                                                                                                                              >  2:238508/1‑150 (MQ=255)                     gggTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAgt                                                                                                                          >  1:660275/1‑150 (MQ=255)                      tgTTGTGTCTTGAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAgtg                                                                                                                         >  2:217044/2‑150 (MQ=255)                       gTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAgtgt                                                                                                                        >  2:645625/1‑150 (MQ=255)                       gTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAgtgt                                                                                                                        >  2:806235/1‑150 (MQ=255)                             tCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGtt                                                                                                                  >  2:679539/1‑150 (MQ=255)                                ttAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTcc                                                                                                               <  1:679539/150‑1 (MQ=255)                                  aaGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAgt                                                                                                                          >  1:650357/1‑137 (MQ=255)                                  aaGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAgt                                                                                                                          <  2:650357/137‑1 (MQ=255)                                  aaGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTggg                                                                                                                     <  2:831292/142‑1 (MQ=255)                                  aaGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTggg                                                                                                                     >  1:831292/1‑142 (MQ=255)                                    gTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGcc                                                                                                                                   <  1:263811/126‑1 (MQ=255)                                    gTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGcc                                                                                                                                   >  2:263811/1‑126 (MQ=255)                                    gTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCAt                                                                                                           <  2:957541/150‑1 (MQ=255)                                     tAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCa                                                                                                                                        >  2:248394/1‑120 (MQ=255)                                     tAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCa                                                                                                                                        <  1:248394/120‑1 (MQ=255)                                       ggAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCAtgtg                                                                                                        >  1:723131/1‑150 (MQ=255)                                          aCTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTgaga                                                                                                     >  1:930212/1‑150 (MQ=255)                                            tACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTcagca                                                                                                                                                     <  2:641708/100‑1 (MQ=255)                                            tACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTcagca                                                                                                                                                     >  1:641708/1‑100 (MQ=255)                                                       gCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTc                                                                                        <  1:536305/150‑1 (MQ=255)                                                                tCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCACGAAATCAGCAGTGAAACGATCGAGCGACGAGGGAAGAGACGAGCTGCGCCAGGGGATAGAAGGCCATCGTCAGGATGCaa                                                                               >  2:403071/1‑150 (MQ=12)                                                                   tAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTga                                                                                                       <  1:16682/123‑1 (MQ=255)                                                                   tAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTga                                                                                                       >  2:16682/1‑123 (MQ=255)                                                                           ggCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGccc                                                                    <  1:238508/150‑1 (MQ=255)                                                                             ccAGCACTCAGAATTTCTTGACGACCATAGAGCATTGGAACAACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCt                                                                  >  2:382336/1‑150 (MQ=255)                                                                                   cacaGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGc                                                            >  2:1215516/1‑150 (MQ=255)                                                                                    acaGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAg                                                                                                 <  2:400623/112‑1 (MQ=33)                                                                                    acaGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAg                                                                                                 >  1:400623/1‑112 (MQ=33)                                                                                    acaGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCg                                                           >  1:795138/1‑150 (MQ=255)                                                                                     caGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCACGATTAAAACCCAAAGCCCCTGTCTGCGa                                                          <  2:660275/150‑1 (MQ=255)                                                                                     caGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGa                                                          <  1:1215516/150‑1 (MQ=255)                                                                                            tCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGAc                                                  <  2:247516/150‑1 (MQ=255)                                                                                              ttGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcgtgcgcagat                                                 >  1:387595‑M2/1‑119 (MQ=255)                                                                                              ttGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcatgtgcagat                                                 <  2:592320‑M2/150‑32 (MQ=255)                                                                                                gacgacCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCCTCAAGATTCAAACCCAAAGCCCCTGTCTGCATC‑GCAGACAggg                                              <  2:930212/150‑1 (MQ=255)                                                                                                 acgacCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcatgcgcagatagg                                              >  2:444323‑M2/1‑116 (MQ=255)                                                                                                  cgacCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcatgtgcagataggg                                             >  1:592320‑M2/1‑115 (MQ=255)                                                                                                  cgacCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcatgcgcagataggg                                             >  2:684987‑M2/1‑115 (MQ=255)                                                                                                  cgacCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcatgcgcagataggg                                             >  1:594142‑M2/1‑115 (MQ=255)                                                                                                  cgacCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTAACACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGc                                            <  1:403071/150‑1 (MQ=255)                                                                                                  cgacCATAGAGCATTCGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACACAAAGCCCCTGTCTGCATC‑GCAGACAGGGGc                                            <  2:892893/150‑1 (MQ=17)                                                                                                     ccATAGAGCATTGGAACCACGTGATCCCACCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCCAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCttt                                         <  1:426358/150‑1 (MQ=255)                                                                                                     ccATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcatgcgcagataggggtt                                          >  2:1162035‑M2/1‑112 (MQ=255)                                                                                                       aTAGAGCATTGGAACCACCCGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcatgcgcagataggggtttt                                        >  2:845038‑M2/1‑110 (MQ=255)                                                                                                        tAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCATC‑GCAGACAGGGGCTTTggg                                      <  1:645625/150‑3 (MQ=255)                                                                                                         agagCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAGACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcatgcgcagatagg                                              <  2:597579‑M2/142‑35 (MQ=255)                                                                                                         agagCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcatgcgcagatagg                                              >  1:597579‑M2/1‑108 (MQ=255)                                                                                                         agagCATTGGAACCACCTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcacaaaacccctacctgcatgcgcagataggggttttgc                                      <  2:1257571‑M2/150‑43 (MQ=255)                                                                                                          gagCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAg                                                <  1:754247/138‑1 (MQ=255)                                                                                                          gagCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAg                                                >  2:754247/1‑138 (MQ=255)                                                                                                          gagCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCtt                                    <  1:806235/150‑1 (MQ=255)                                                                                                                 ggAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTCCggg                             >  1:953820/1‑150 (MQ=255)                                                                                                                  gAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTCCGGGt                            >  2:1076035/1‑150 (MQ=255)                                                                                                                       accTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTCCGGGTTTCaa                       >  2:670261/1‑150 (MQ=255)                                                                                                                       accTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTCCGGGTTTCaa                       <  1:42582/150‑1 (MQ=255)                                                                                                                       accTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTCCCCCAAGTCAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTCCGGGTTTCaa                       <  1:1185636/150‑1 (MQ=255)                                                                                                                        ccTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcgtgcgc                                                     >  2:577162‑M2/1‑93 (MQ=255)                                                                                                                        ccTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcgtgcgc                                                     <  1:577162‑M2/120‑28 (MQ=255)                                                                                                                        ccTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcatgcgcagataggg                                             >  2:59377‑M2/1‑93 (MQ=255)                                                                                                                        ccTGATCCCATCCCGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcatgcgcagataggg                                             <  1:59377‑M2/128‑36 (MQ=255)                                                                                                                        ccTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCttt                                         >  2:478464/1‑131 (MQ=255)                                                                                                                        ccTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCttt                                         <  1:478464/131‑1 (MQ=255)                                                                                                                         cTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAata                     >  1:644415/1‑150 (MQ=255)                                                                                                                               ccatcccGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcatgcgcagataggg                                             >  1:668149‑M2/1‑86 (MQ=255)                                                                                                                               ccatcccGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcgcaaaacccctacctgcatgcgcagataggg                                             <  2:668149‑M2/121‑36 (MQ=255)                                                                                                                               ccatcccGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGtt               <  1:670261/150‑1 (MQ=255)                                                                                                                               ccatcccGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGtt               >  2:1278422/1‑150 (MQ=255)                                                                                                                                catcccGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTa              >  1:269817/1‑150 (MQ=255)                                                                                                                                  tcccGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcacaaaacccctacctgcatgcgcagataggg                                             <  2:641080‑M2/118‑36 (MQ=255)                                                                                                                                  tcccGAACTCAGTAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAtttcacaaaacccctacctgcatgcgcagataggg                                             >  1:641080‑M2/1‑83 (MQ=255)                                                                                                                                    ccGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAAc          >  1:245457/1‑150 (MQ=255)                                                                                                                                     cGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAggg                                              <  2:1274318/113‑1 (MQ=255)                                                                                                                                     cGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAggg                                              >  1:1274318/1‑113 (MQ=255)                                                                                                                                     cGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACt         >  1:848934/1‑150 (MQ=255)                                                                                                                                     cGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACt         >  1:1008261/1‑150 (MQ=255)                                                                                                                                       aaCTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGa       <  2:1008261/150‑1 (MQ=255)                                                                                                                                          tCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGC‑ATCGCAGACAgg                                               <  2:1117888/107‑1 (MQ=17)                                                                                                                                          tCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGC‑ATCGCAGACAgg                                               >  1:1117888/1‑107 (MQ=18)                                                                                                                                            agcagTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCt                                           >  2:1066444/1‑109 (MQ=255)                                                                                                                                            agcagTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCt                                           <  1:1066444/109‑1 (MQ=255)                                                                                                                                            agcagTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCt  <  2:795138/150‑1 (MQ=255)                                                                                                                                              cagTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTCCGGGt                            >  1:279174/1‑122 (MQ=255)                                                                                                                                              cagTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTCCGGGt                            <  2:279174/122‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                     CTATCTGCACATGCAGGTAGGGGTTTTGTCTTTAAGTAGGAACTACAGAGATTCGCAGGCACGTCCTAGGGACGGGCCAGCACACAGAATTTCTTGACGACCATAGAGCATTGGAACCACCTGATCCCATCCCGAACTCAGCAGTGAAACGATGCATCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCAAACCCAAAGCCCCTGTCTGCGAT‑GCAGACAGGGGCTTTGTCTTTTCCGGGTTTCAATATCGGTTAGAACTGATAGCT  >  NC_002947/181802‑182090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑