| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,253,439 | G→C | 6.9% | intergenic (‑79/+28) | PP_1096 ← / ← metG | electron transport complex protein/methionine‑‑tRNA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,253,439 | 0 | G | C | 6.9% | 174.6 / 4.4 | 59 | intergenic (‑79/+28) | PP_1096/metG | electron transport complex protein/methionine‑‑tRNA ligase |
| Reads supporting (aligned to +/- strand): ref base G (22/32); new base C (2/2); total (25/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01 | |||||||||||
AGTGGCTGTCGGAGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGCCAAAGCGCATCTTCCG > NC_002947/1253290‑1253583 | agTGGCCGTCGGAGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAgg < 2:36360/150‑1 (MQ=255) gTGGCTGTCGGAGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGc < 1:1117108/150‑1 (MQ=255) gCTGTCGGAGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGTTTACGATA‑CCCCGATTCTGGGGCGAGTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGATCGGGCAGTCCGc > 1:414995/1‑150 (MQ=255) cTGTCGGAGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCt < 1:1011916/150‑1 (MQ=255) tGTCGGAGCAGGCACAGGTGATTGATCAGTTCGGCGCTTATCAGGACCAGGATTTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGCTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGcat < 1:685600/150‑3 (MQ=255) tGTCGGAGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTc < 1:1133897/150‑1 (MQ=255) gTCGGAGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTcc < 1:294737/150‑1 (MQ=255) cGGAGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCca < 2:1274479/150‑1 (MQ=255) caGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGTTTGTCAGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGc < 2:93788/150‑1 (MQ=255) aGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCa < 1:237181/150‑1 (MQ=255) ggTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAg < 2:174691/150‑1 (MQ=255) gTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGCCTGCCCGCTccc < 1:709320/135‑1 (MQ=255) gTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGCCTGCCCGCTccc > 2:709320/1‑135 (MQ=255) gTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGCCTGCCCGCTCCCACAAttacgcag > 2:109440/1‑140 (MQ=39) gTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGCCTGCCCGCTCCCACAAttacgcag < 1:109440/147‑8 (MQ=39) tgattgatCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTcc > 2:670989/1‑133 (MQ=255) tgattgatCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTcc < 1:670989/133‑1 (MQ=255) gattgatCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCa < 2:514679/146‑1 (MQ=255) gattgatCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCa > 1:514679/1‑146 (MQ=255) atCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTa < 1:959676/150‑1 (MQ=255) tCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTAc > 1:131067/1‑150 (MQ=255) gTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGTGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTg < 1:187342/150‑1 (MQ=255) gTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTg < 1:849793/150‑1 (MQ=255) cggcgCTGATAAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGAGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATg < 2:1171799/150‑1 (MQ=255) cTGATCAGGACCAGGCTGTAGTAATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGctg < 2:976395/150‑1 (MQ=255) cTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGctg > 1:331230/1‑150 (MQ=255) gATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGctggc > 2:271518/1‑150 (MQ=255) aTCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGctggcc > 2:672209/1‑150 (MQ=255) aCCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCtt < 2:131067/150‑1 (MQ=255) gATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGgcgc > 1:1111361/1‑150 (MQ=255) gATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGgcgc < 1:99785/150‑1 (MQ=255) aGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTg < 2:603932/150‑1 (MQ=255) tcattcatGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTc > 2:567437/1‑150 (MQ=255) ttcctGAGAGGACGATGGCCGGGAATC‑GGGGCGATTGTCGGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCgg < 2:673191/150‑1 (MQ=255) ttcatGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCgg > 1:463835/1‑150 (MQ=255) ttcatGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCgg < 2:414995/150‑1 (MQ=255) tggttGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCgg > 2:343221/5‑150 (MQ=255) gagaaCGATAGCCGGGACTT‑GGCGCGATTGTCCGGGTTTGACATGAAGGGGTCTGCGTAATTGTGGGAGGGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTcagc < 2:342019/146‑1 (MQ=255) gACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCGGGGCTCAGCAgg > 1:450706/1‑150 (MQ=255) gATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAg < 2:331230/150‑1 (MQ=255) tAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGAt < 1:271518/150‑1 (MQ=255) ccGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATttc > 2:707804/1‑150 (MQ=255) ggAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGTGCGGATGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATttcttc > 2:104016/1‑150 (MQ=255) ggAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGctggcct < 1:591872/109‑1 (MQ=255) ggAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGctggcct > 2:591872/1‑109 (MQ=255) aaTC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCAc < 1:672209/150‑1 (MQ=255) gCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAgg > 2:1194865/1‑150 (MQ=255) tCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCa < 1:811035/150‑1 (MQ=255) gTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCGGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTCGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCCGGGCCGGCCGCCAt > 2:439040/1‑150 (MQ=255) tttGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAg < 1:164632/63‑1 (MQ=255) tttGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAg > 2:164632/1‑63 (MQ=255) cATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCAt < 1:759283/150‑1 (MQ=255) aTGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATg > 1:1148119/1‑150 (MQ=255) ggggCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTc < 1:567437/150‑1 (MQ=255) cgtGCGTAATTGTGGGAGCGGACAGGCCGCTCCCATAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCTCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGac < 2:24733/148‑1 (MQ=255) gggCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGCCAAAGCGCATCTTcc > 2:293722/1‑150 (MQ=255) gggCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGCCAAAACGCATCTTcc > 2:552976/1‑150 (MQ=255) ggCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGc > 2:597947/1‑134 (MQ=255) ggCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGc < 1:597947/134‑1 (MQ=255) ggCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGCCAAAGCGCATCTTCCg > 1:1111262/1‑150 (MQ=255) | AGTGGCTGTCGGAGCAGGCACAGGTGATTGATCAGTGCGGCGCTGATCAGGACCAGGATGTAGTCATTCATGAGATGACGATAGCCGGGAATC‑GGGGCGATTGTCCGGGTTTGACATGAAGGGGCCTGCGTAATTGTGGGAGCGGGCAGGCCGCTCCCACAATGGCCCGCAGGGCGGTTACTTGATGCGCTGGCCTGGCTTGGCGCCGCTGTCCGGGCTCAGCAGGTAGATTTCTTCACCGCCAGGGCCGGCAGCCATGACCATGCCTTCGGACACGCCAAAGCGCATCTTCCG > NC_002947/1253290‑1253583 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |