| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,311,752 | G→C | 7.7% | intergenic (‑214/+64) | PP_1144 ← / ← rapA | membrane protein/RNA polymerase‑associated protein RapA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,311,752 | 0 | G | C | 7.7% | 157.1 / 4.8 | 52 | intergenic (‑214/+64) | PP_1144/rapA | membrane protein/RNA polymerase‑associated protein RapA |
| Reads supporting (aligned to +/- strand): ref base G (27/21); new base C (2/2); total (29/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01 | |||||||||||
TATAGGAAGGAGTACTTAAGCCTTCCTGACTAATGGCACAATGATTTCTACGATTTGGTTATAACTCCAATCGCAGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGGCGAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAGGCGCAGGGCGGCCTTCTCCAGCATTGCCAGGCCATCTTCGCGCCGCTTGCG > NC_002947/1311605‑1311899 | tataGGAAGGAGTACTTAAGCCTTCCTGACTAATGGCACAATGATTTCTACGATTTGGTTATAACTCCAATCGCAGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGGCGAGCCCGCGAAGCATCCACTGCGGTGGAt > 1:182836/1‑150 (MQ=255) tACTTAAGCCTTCCTGACTAATGGCACAATGATTTCTACGATTTGGTTATAACTCCAATCGCAGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGGCGAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCttt < 2:989482/150‑1 (MQ=255) cTTAAGCCTTCCTGACTAATGGCACAACGATTTCTACGATTTGGTTATAACTCCAATCGCAGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGGCGAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGc < 2:299188/150‑1 (MQ=255) cTTAAGCCTTCCTGACAAATGGCACAATGATTTCTACGATTTGGTTATAACTCCAATCGCAGTGAGGAATAAATTTGGGGCACTAATGCCTTCCTGTGGGAGCCGGCGAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGc < 1:105060/150‑1 (MQ=255) ccTTCCTGACTAATGGCACAATGATTTCTACGATTTGGTTATAACTCCAATCGCAGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGGCGAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGgtg > 1:1260619/1‑149 (MQ=255) ccTTCCTGACTAATGGCACAATGATTTCTACGATTTGGTTATAACTCCAATCGCAGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGGCGAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGgtg < 2:1260619/149‑1 (MQ=255) ccTTCCTGACTAATGGCACAATGATTTCTACGATTTGGTTATAACTCCAATCGCAGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGGCGAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGT > 2:813688‑M1/1‑150 (MQ=255) cTGACTAATGGCACAATGATTTCTACGATTTGGTTATAACTCCAATCGCAGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGGCGAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCG > 1:1168395‑M1/1‑150 (MQ=255) aTGGCACAATGATTTCTACGATTTGGTTATAACTCCAATCGCAGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGGCGAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGc > 1:530368/1‑150 (MQ=255) cacaATGATTTCTACGATTTGGTTATAACTCCAATCGCAGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGGCGAGCCCGCGCACCATCCACTCCGGGGGATGCCCCCGGCTTTTCCGGTGTTTGCGGGCGGGCCCGc > 2:959501/1‑150 (MQ=255) acaATGATTTCTACGATTTGGTTATAACTCCAATCGCAGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGGCGAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGGTTTTCGCGGGCGTGCCCGCt > 2:209780/1‑150 (MQ=255) caATGATTTCTACGATTTGGTTATAACTCCAATCGCAGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGGCGAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTc < 2:953773/150‑1 (MQ=255) tGGTTATAACTCCAATCGCAGTGAGGAATAATTTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGGCGAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCgggg > 2:789530/1‑150 (MQ=255) cAATCGCAGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGGCGAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTagcc > 1:172502/1‑150 (MQ=255) aaTCGCAGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGGCGAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTagcca > 2:511853/1‑150 (MQ=255) aaTCGCAGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGGCGAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTagcca < 2:182836/150‑1 (MQ=255) cAGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGGCGAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTAGCCAGccac > 1:569326/1‑150 (MQ=255) aGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGGCGAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTAGCCAGccacc < 2:1168395/150‑1 (MQ=255) tGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGGCGAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTAGCCAGCCACCAg > 2:1114511/1‑150 (MQ=255) aaTAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGGCGAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGGCTGCGCTAGCCAGCCACCAGCAcgc > 1:1284811/1‑150 (MQ=255) tAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGGCGAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCcac < 2:302419/109‑1 (MQ=255) tAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGGCGAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCcac > 1:302419/1‑109 (MQ=255) ccTAATGCCTTCCTGTGGGAGCGGGCGAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAgg < 1:821664/150‑1 (MQ=255) ccTAATGCCTTCCTGTGGGAGCGGGCGAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAgg > 2:221226/1‑150 (MQ=255) gCCTTCCTGTGGGAGCGGGCGAGCCGGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAGGCGCAgg < 1:209780/150‑1 (MQ=255) gCCTTCCTGTGGGAGCGGGCGAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAGGCGCAgg > 1:25794/1‑150 (MQ=255) ttCCTGTGGGAGCGGGCGAGCCCGACCACCATCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAGGCGCAGggcg > 1:359420/1‑150 (MQ=255) ccTGTGGGAGGGGGCGAGCTCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAGGCGCAGggcggc < 1:959501/150‑1 (MQ=255) ccTGTGGGAGCGGGCGAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAGGCGCAGggcggc < 1:135520/150‑1 (MQ=255) ggAGCGGGCGAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAGGCGCAGGGCGGCCTtctc < 2:98760/150‑1 (MQ=255) ggCGAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAGGCGCAGGGCGGCCTTCTCCAGCAt > 1:353720/1‑150 (MQ=255) gCGAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCTGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAGGCGCAGGGCGGCCTTCTCCAGCAtt < 2:359420/150‑1 (MQ=255) gAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAGGCGCAGGGCGGCCTTCTCCAGCATTGc < 1:400163/150‑1 (MQ=255) ccGCGAAGCAAGCACTGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCaagcccgctcccacagggtaaggtgctgcggtttagactttgtggtacagctggctgccttcctggcggaac > 1:662931‑M1/1‑53 (MQ=255) ccGCGAAGCAAGCACTGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCaagcccgctcccacagggtaaggtgctgcggtttagactttgtggtacagctggctgccttcctggcggaac < 2:662931‑M1/125‑73 (MQ=255) ccGCGAAGCAAGCACTGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGCGCaagcccgctcccacagggtgaggtgctgcggtttagactttgtggtacagctggctgccttcctggcggaaccgctcggcctgctcacgcatgcctt > 1:733249‑M1/1‑53 (MQ=255) tcgAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAGGCGCAGGGCGGCCTTCTCCAGCATTGCCAGGcc > 2:882004/2‑150 (MQ=255) gcgAAGCAAGCACTGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCaagcccgctcccacagggtgaggtgctgcggtttagactttgtggtacagctggctgccttcctggcggaaccgctcggcctgctcacgcatgccttgc < 2:733249‑M1/150‑100 (MQ=255) tggCATCCACCGCAGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAGGCGCAGGGCGGCCTTCTCCAGCATTGCCAGGCCATc > 2:859016/3‑150 (MQ=255) ggCATCCACCGCAGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAGGCGCAGGGCGGCCTTCTCCAGCATTGCCAGGCCATCt > 1:270109/2‑150 (MQ=255) aTCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAGGCGCAGGGCGGCCTTCTCCAGCATTGcc > 1:314221/1‑138 (MQ=255) aTCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAGGCGCAGGGCGGCCTTCTCCAGCATTGcc < 2:314221/138‑1 (MQ=255) aTCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAGGCGCAGGGCGGCCTTCTCCAGCATTGCCAGGCCATCTTcg > 1:761240/1‑150 (MQ=255) tCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTagc > 2:839141/1‑81 (MQ=255) tCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTagc < 1:839141/81‑1 (MQ=255) tCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTag > 2:1080943/1‑80 (MQ=255) tCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTag < 1:1080943/80‑1 (MQ=255) tCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAGgcgc > 2:959353/1‑113 (MQ=255) tCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAGgcgc < 1:959353/113‑1 (MQ=255) caccgcagTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAGGCGCAGGGCGGCCTTCTCCAGCATTGCCAGGCCATCTTCgcgc < 2:270109/143‑1 (MQ=255) tGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAGGCGCAGGGCGGCCTTCTCCAGCATTGCCAGGCCATCTTCGCGCCGCTTgcg < 2:353720/150‑1 (MQ=255) tGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAGGCGCAGGGCGGCCTTCTCCAGCATTGCCAGGCCATCTTCGCGCCGCTTgcg > 2:292433/1‑150 (MQ=255) | TATAGGAAGGAGTACTTAAGCCTTCCTGACTAATGGCACAATGATTTCTACGATTTGGTTATAACTCCAATCGCAGTGAGGAATAAATTTGGGGCCCTAATGCCTTCCTGTGGGAGCGGGCGAGCCCGCGAAGCATCCACTGCGGTGGATGCCACCGGCTTTGCCGGTGTTCGCGGGCGTGCCCGCTCCCACAAGGGGCGCGGGGCCTGCGCTAGCCAGCCACCAGCACGCGAATGGCTTCCAGGCGCAGGGCGGCCTTCTCCAGCATTGCCAGGCCATCTTCGCGCCGCTTGCG > NC_002947/1311605‑1311899 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |