Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,375,668 G→A 8.7% A428T (GCC→ACC)  kup → potassium transport system protein Kup

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,375,6680GA 8.7% 123.6 / 3.1 46A428T (GCC→ACC) kuppotassium transport system protein Kup
Reads supporting (aligned to +/- strand):  ref base G (20/22);  new base A (2/2);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01

CGGTACCATGCTGATCACCACCGTCCTGGTGTCAGCGGTGATGCTGCTGCTGTGGAAGTGGCCGCCGCTGCTCGCAGTGCCGATTCTGGTCGGCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTACCTGCTGATGAGCACCTGGAAGCGCGGCAAGCAGATTCTGGTCGAGCGCATCGATGAAGGGGCGCTGCCATTGCCATTGTTCATCAGC  >  NC_002947/1375544‑1375817
                                                                                                                            |                                                                                                                                                      
cGGTACCATGCTGATCACCACCGTCCTGGTGTCAGCGGTGATGCTGCTGCTGTGGAAGTGGCCGCCGCTGCTCGCAGTGCCGATTCTGGTCGGCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAggg                                                                                                                              >  2:311291/1‑150 (MQ=255)
cGGTACCATGCTGATCACCACCGTCCTGGTGTCAGCGGTGATGCTGCTGCTGTGGAAGTGGCCGCCGCTGCTCGCAGTGCCGATTCTGGTCGGCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAggg                                                                                                                              <  1:311291/150‑1 (MQ=255)
      cATGCTGATCACCACCGTCCTGGTGTCAGCGGTGATGCTGCTGCTGTGGAAGTGGCCGCCGCTGCTCGCAGTGCCGATTCTGGTCGGCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGc                                                                                                                        <  1:780487/150‑1 (MQ=255)
      cATGCTGATCACCACCGTCCTGGTGTCAGCGGTGATGCTGCTGCTGTGGAAGTGGCCGCCGCTGCTCGCAGTGCCGATTCTGGTCGGCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGc                                                                                                                        >  2:293705/1‑150 (MQ=255)
      cATGCTGATCACCACCGTCCTGGTGTCAGCGGTGATGCTGCTGCTGTGGAAGTGGCCGCCGCTGCTCGCAGTGCCGATTCTGGTCGGCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGc                                                                                                                        <  1:204102/150‑1 (MQ=255)
           tGATCACCACCGTCCTGGTGTCAGCGGTGATGCTGCTGCTGTGGAAGTGGCCGCCGCTGCTCGCAGTGCCGATTCTGGTCGGCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTcc                                                                                                                   >  2:1063207/1‑150 (MQ=255)
               caccacCGTCCTGGTGTCAGCGGTGATGCTGCTGCTGTGGAAGTGGCCGCCGCTGCTCGCAGTGCCGATTCTGGTCGGCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGgt                                                                                                               <  2:205438/150‑1 (MQ=255)
                  caccGTCCTGGTGTCAGCGGTGATGCTGCTGCTGTGGAAGTGGCCGCCGCTGCTCGCAGTGCCGATTCTGGTCGGCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGtt                                                                                                            <  2:155410/150‑1 (MQ=255)
                  caccGTCCTGGTGTCAGCGGTGATGCTGCTGCTGTGGAAGTGGCCGCCGCTGCTCGCAGTGCCGATTCTGGTCGGCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCCGTGtt                                                                                                            >  1:479939/1‑150 (MQ=255)
                        tggcttgtCAGCGGTGATGCTGCTGCTGTGGAAGTGGCCGCCGCTGCTCGCAGTGCCGATTCTGGTCGGCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCgg                                                                                                      <  1:1111981/145‑1 (MQ=255)
                         cTGGTGTCAGCGGTGATGCTGCTGCTGTGGAAGTGGCCGCCGCTGCTCGCAGTGCCGATTCTGGTCGGCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGAGTTGGCCggt                                                                                                     >  2:482755/1‑150 (MQ=255)
                                 aGCGGTGATGCTGCTGCTGTGGAAGTGGCCGCCGCTGCTCGCAGTGCCGATTCTGGTCGGCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCt                                                                                             >  2:629040/1‑150 (MQ=255)
                                     gTGATGCTGCTGCTGTGGAAGTGGCCGCCGCTGCTCGCAGTGCCGATTCTGGTCGGCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTAc                                                                                         <  1:422634/150‑1 (MQ=255)
                                            tgctgctgTGGAAGTGGCCGCCGCTGCTCGCAGTGCCGATTCTGGTCGGCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGcc                                                                                                                                    <  1:314393/101‑1 (MQ=255)
                                            tgctgctgTGGAAGTGGCCGCCGCTGCTCGCAGTGCCGATTCTGGTCGGCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGcc                                                                                                                                    >  2:314393/1‑101 (MQ=255)
                                              ctgctgTGGAAGTGGCCGCCGCTGCTCGCAGTGCCGATTCTGGTCGGCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCACCAACGTGCCGAAAACCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTACCTGCtgatg                                                                                >  1:159932/1‑150 (MQ=255)
                                               tgctgTGGAAGTGGCCGCCGCTGCTCGCAGTGCCGATTCTGGTCGGCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTACCTGCtgatga                                                                               >  1:1251724/1‑150 (MQ=255)
                                                 ctgTGGAAGTGGCCGCCGCTGCTCGCAGTGCCGATTCTGGTCGGCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTACCTGCTGATGAGc                                                                             <  2:1251724/150‑1 (MQ=255)
                                                  tgtgGAAGTGGCCGCCGCTGCTCGCAGTGCCGATTCTGGTCGGCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTACCTGCTGATGAGCa                                                                            >  2:267827/1‑150 (MQ=255)
                                                    tgGAAGTGGCCGCCGCTGCTCGCAGTGCCGATTCTGGTCGGCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGtgtg                                                                                               <  2:65719/129‑1 (MQ=255)
                                                    tgGAAGTGGCCGCCGCTGCTCGCAGTGCCGATTCTGGTCGGCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGtgtg                                                                                               >  1:65719/1‑129 (MQ=255)
                                                                         gCAGTGCCGATTCTGGTCGGCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTACCTGCTGATGAGCACCTGGAAGCGCGGCAAGCAGAtt                                                     >  1:190789/1‑150 (MQ=255)
                                                                             tGCCGATTCTGGTCGGCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGAACATTGTCAGGCCTATGTGCTCATCAGTACCGTGAAGCTCGGCAAGCAGATTCTgg                                                 <  1:482755/150‑1 (MQ=255)
                                                                                 gATTCTGGTCGGCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTACCTGCTGATGAGCACCTGGAAGCGCGGCAAGCAGATTCTGGTCGa                                             <  1:629040/150‑1 (MQ=255)
                                                                                   ttCTGGTCGGCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTACCTGCTGATGAGCACCTGGAAGCGCGGCAAGCAGATTCTGGTCGAgc                                           <  1:151026/150‑1 (MQ=255)
                                                                                     cTGGTCGGCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTACCTGCTGATGAGCACCTGGAAGCGCGGCAAGCAGATTCTGGTCGAgcgc                                         <  1:734004/150‑1 (MQ=255)
                                                                                      tGGTCGGCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCACCAACGTGCCGAAAACCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTACCTGCTGATGAGCACCTGGAAGCGCGGCAAGCAGATTCTGGTCGAGCGCa                                        <  2:159932/150‑1 (MQ=255)
                                                                                           ggCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTACCTGCTGATGAGCACCTGGAAGCGCGGCAAGCAGATTCTGGTCGAGCGCATCGAt                                   >  1:1160911/1‑150 (MQ=255)
                                                                                            gCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCCACAGCG‑GGTGCCTTCCCGGTGTGGGCCGGTGGTGTGCTGTACCTGCTGATGAGCACCTGGAAGCGCGGCAAGCAGATTCTGGTCGAGCGCATCGATg                                  >  1:478851/1‑150 (MQ=255)
                                                                                              ttCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTACCTGCTGATGAGCACCTGGAAGCGCGGCAAGCAGATTCTGGTCGAGCGCATCGATGaa                                >  2:972313/1‑150 (MQ=255)
                                                                                                  tgctgGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTAc                                                                                         <  1:508597/89‑1 (MQ=255)
                                                                                                  tgctgGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTAc                                                                                         >  2:508597/1‑89 (MQ=255)
                                                                                                       gTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTACCTGCTGATGAGCACCTGGAAGCGCGGCAAGCAGAtt                                                     <  2:1107202/120‑1 (MQ=255)
                                                                                                       gTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTACCTGCTGATGAGCACCTGGAAGCGCGGCAAGCAGAtt                                                     >  1:1107202/1‑120 (MQ=255)
                                                                                                            cGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTACCTGCTGATGAGCACCTGGAAGCGCGGCAAGCAGATTCTGGTCGAGCGCATCGATGAAGGGGCGCtgccatt                  <  2:1160911/150‑1 (MQ=255)
                                                                                                                ttGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTACCTGCTGATGAGCACCTGGAAGCGCGGCAAGCAGATTCTGGTCGAGCGCATCGATGAAGGGGCGCtgccattgcca              <  2:479939/149‑1 (MQ=255)
                                                                                                                cTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTACCTGCTGATGAGCACCTGGAAGCGCGGCAAGCAGATTCTGGTCGAGCGCATCGATGAAGGGGCGCtgccattgcca              >  2:550385/1‑150 (MQ=255)
                                                                                                                 tGTTCTTCGCCACCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTACCTGCTGATGAGCGCCTGGAAGCGCgg                                                               <  2:450269/100‑1 (MQ=255)
                                                                                                                 tGTTCTTCGCCACCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTACCTGCTGATGAGCACCTGGAAGCGCgg                                                               >  1:450269/1‑100 (MQ=255)
                                                                                                                   ttcttcGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTACCTGCTGATGAGCACCTGGAAGCGCGGCAAGCAGATTCTGGTCGAgc                                           <  2:1126719/118‑1 (MQ=255)
                                                                                                                   ttcttcGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTACCTGCTGATGAGCACCTGGAAGCGCGGCAAGCAGATTCTGGTCGAgc                                           >  1:1126719/1‑118 (MQ=255)
                                                                                                                   ttcttcGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTACCTGCTGATGAGCACCTGGAAGCGCGGCAAGCAGATTCTGGTCGAGCGCATCGATGAAGGGGCGCTGccattgccattg           <  1:267827/150‑1 (MQ=255)
                                                                                                                   ttcttcGCAGACAACGTGGCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTACCTGGTGATTAGCACCTGGAAGCGCGGCAAGCAGATTCTGGTCGAGCGCATCGATGAAGGGGCGCTGccattgccattg           <  2:190789/150‑1 (MQ=255)
                                                                                                                     cttcGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTACCTGCTGATGAGCACCTGGAAGCGCGGCAAGCAGATTCTGGTAGAGCGCATCGATGAAGGGGCGCTGCCATTGCCATTGtt         >  1:487189/1‑150 (MQ=255)
                                                                                                                      ttcGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTACCTGCTGATGAGCACCTGGAAGCGCGGCAAGCAGATTCTGGTCGAGCGCATCGa                                    >  2:103944/1‑122 (MQ=255)
                                                                                                                      ttcGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTACCTGCTGATGAGCACCTGGAAGCGCGGCAAGCAGATTCTGGTCGAGCGCATCGa                                    <  1:103944/122‑1 (MQ=255)
                                                                                                                          ccgccAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGTTGTGCTGTACCTGCTGATGAGCACCTGGAAGcgcg                                                                <  1:1048727/90‑1 (MQ=255)
                                                                                                                          ccgccAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGTTGTGCTGTACCTGCTGATGAGCACCTGGAAGcgcg                                                                >  2:1048727/1‑90 (MQ=255)
                                                                                                                           cgccAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTACCTGCTGATGAGCACCTGGAAGCGCGGCAAGCAGATTCTGGTCGAGCGCATCGATGAAGGGGCGCTGCCATTGCCATTGTTCATcag   <  1:972313/150‑1 (MQ=255)
                                                                                                                            gccAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTACCTGCTGATGAGCACCTGGAAGCGCGGCAAGCAGATTCTGGTCGAGCGCATCGATGAAGGGGCGCTGCCATTGCCATTGTTCATcagc  >  1:845083/1‑150 (MQ=255)
                                                                                                                            |                                                                                                                                                      
CGGTACCATGCTGATCACCACCGTCCTGGTGTCAGCGGTGATGCTGCTGCTGTGGAAGTGGCCGCCGCTGCTCGCAGTGCCGATTCTGGTCGGCTTCCTGCTGGTCGACGGGCTGTTCTTCGCCGCCAACGTGCCGAAAATCGCC‑CAGGGCGGTGCCTTCCCGGTGTTGGCCGGTGGTGTGCTGTACCTGCTGATGAGCACCTGGAAGCGCGGCAAGCAGATTCTGGTCGAGCGCATCGATGAAGGGGCGCTGCCATTGCCATTGTTCATCAGC  >  NC_002947/1375544‑1375817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑