Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,416,949 G→T 12.5% G64V (GGG→GTG)  PP_1239 → metallo‑beta‑lactamase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,416,9490GT12.5% 130.4 / 7.2 48G64V (GGG→GTG) PP_1239metallo‑beta‑lactamase family protein
Reads supporting (aligned to +/- strand):  ref base G (21/21);  new base T (3/3);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

GCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCATATCGGCAGCCTGGAAGTAACCGCAGCGCG  >  NC_002947/1416810‑1417096
                                                                                                                                           |                                                                                                                                                   
gCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTg                                                                                                                                           <  1:653949/150‑1 (MQ=255)
    gtggtgACGCGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGt                                                                                                                                       >  1:579066/1‑150 (MQ=255)
            acGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCCCATGCACGTGGTCGGca                                                                                                                                             <  2:1197863/136‑2 (MQ=255)
            acGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCCCATGCACGTGGTCGGca                                                                                                                                             >  1:1197863/1‑135 (MQ=255)
               ttCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTAc                                                                                                                            <  2:1119797/150‑1 (MQ=255)
                 aaTCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACaa                                                                                                                          <  1:217409/149‑1 (MQ=255)
                      tGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCATCCCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTAc                                                                                                                     >  2:109186/1‑150 (MQ=255)
                      tGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCAGATCGAGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTAc                                                                                                                     >  2:314806/1‑150 (MQ=255)
                             ttGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTa                                                                                                              >  2:1116379/1‑150 (MQ=255)
                                  gCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCa                                                                                                         >  1:996621/1‑150 (MQ=255)
                                   cTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAg                                                                                                        >  1:221920/1‑150 (MQ=255)
                                    ttttCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAAt                                                                                                                         <  2:1040012/132‑1 (MQ=255)
                                    ttttCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCGCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAAt                                                                                                                         >  1:1040012/1‑132 (MQ=255)
                                         ccTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAgg                                                                                                  <  1:1251112/150‑1 (MQ=255)
                                          cTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAggg                                                                                                 >  1:596699/1‑150 (MQ=255)
                                              gTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACtt                                                                                             >  1:804800/1‑150 (MQ=255)
                                                  aaCCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTgcg                                                                                         >  1:233391/1‑150 (MQ=255)
                                                    ccGAGCAGCGCCAGGCGCCGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAgg                                                                                                  <  2:234866/139‑1 (MQ=255)
                                                    ccGAGCAGCGCCAGGCGCCGCTCGGGGCTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAgg                                                                                                  >  1:234866/1‑139 (MQ=255)
                                                      gAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGc                                                                                     >  2:414869/1‑150 (MQ=255)
                                                                    gctgctCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGt                                                                       >  1:198876/1‑150 (MQ=255)
                                                                       gctCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGa                                                                    <  1:1116379/150‑1 (MQ=255)
                                                                         tCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAgg                                                                  >  1:7775/1‑150 (MQ=255)
                                                                              gTTTCTGCAGCTCAGCTCAGTGCAGTGCTTGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGcc                                                             <  1:245394/150‑1 (MQ=255)
                                                                                ttCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCgg                                                           <  1:173594/150‑1 (MQ=255)
                                                                                  cTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTc                                                                                                                                                 >  1:890086/1‑62 (MQ=255)
                                                                                  cTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTc                                                                                                                                                 <  2:890086/62‑1 (MQ=255)
                                                                                   tGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGttt                                                        <  1:1267052/150‑1 (MQ=255)
                                                                                           agctcagTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCgt                                                <  2:7775/150‑1 (MQ=255)
                                                                                               cagtgcagtgCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTgg                                            <  2:221920/150‑1 (MQ=255)
                                                                                                   gcagtgcTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCg                                                                                                                   >  1:761983/1‑75 (MQ=255)
                                                                                                   gcagtgcTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCg                                                                                                                   <  2:761983/75‑1 (MQ=255)
                                                                                                     agtgcTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCAGGTTTTCTCGCGTGTGGCCAAAg                                      <  2:804800/150‑1 (MQ=255)
                                                                                                      gtgcTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGc                                     <  1:420136/150‑1 (MQ=255)
                                                                                                          tGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGc                                 >  2:5372/1‑150 (MQ=255)
                                                                                                           ggTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCa                                >  1:253601/1‑150 (MQ=255)
                                                                                                            gTCACCCACGAACATACCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCat                               >  1:709940/1‑150 (MQ=255)
                                                                                                               aCCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCTTGCATATc                            <  2:442763/150‑1 (MQ=255)
                                                                                                                      agcaagCCGACCCCATGCACGTGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGtgtg                                             >  2:471043/6‑126 (MQ=255)
                                                                                                                      agcaagCCGACCCCATGCACGTGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGtgtg                                             <  1:471043/121‑1 (MQ=255)
                                                                                                                         aagCCGACCCCATGCACGTGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCg                                                            <  2:1019398/106‑1 (MQ=255)
                                                                                                                         aagCCGACCCCATGCACGTGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCg                                                            >  1:1019398/3‑108 (MQ=255)
                                                                                                                         aTGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTtc                                                      >  1:1022095/1‑114 (MQ=255)
                                                                                                                         aTGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTtc                                                      <  2:1022095/114‑1 (MQ=255)
                                                                                                                          tGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCaaa                                       <  1:94462/128‑1 (MQ=255)
                                                                                                                          tGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCaaa                                       >  2:94462/1‑128 (MQ=255)
                                                                                                                           gCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCATATCGGCAGCCTGGaa                <  2:996621/150‑1 (MQ=255)
                                                                                                                                         tGGCGTCGGTTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCATATCGGCAGCCTGGAAGTAACCGCAGcgcg  <  1:511791/150‑1 (MQ=255)
                                                                                                                                           gggTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGc                                                                           >  2:185046/1‑75 (MQ=255)
                                                                                                                                           gggTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGc                                                                           <  1:185046/75‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                   
GCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTGGAGGTGGCCGGTTTTCTCGCGTGTGGCCAAAGCCTGCATATCGGCAGCCTGGAAGTAACCGCAGCGCG  >  NC_002947/1416810‑1417096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑