| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,528,549 | G→A | 11.3% | R283H (CGC→CAC) | ftsA → | cell division protein FtsA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,528,549 | 0 | G | A | 11.3% | 114.5 / 4.5 | 44 | R283H (CGC→CAC) | ftsA | cell division protein FtsA |
| Reads supporting (aligned to +/- strand): ref base G (21/18); new base A (2/3); total (23/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTGGCGACCAGGTGACCAACGACATCGCCATGGCCCTGCGTACCCCGACCCAGTACGCCGAAGAAATCAAGATCCGCTACGCCTGTGCCCTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGACGAGCTCTTCACCCTTATCCAGGCCGAACTGCGCCGCAGTGGCTACGAAGACCTGGTGCCCGCCGGCATCGTCCTCACCGGCGGCACCG > NC_002947/1528404‑1528695 | gCTGGCGACCAGGTGACCAACGACATCGCCATGGCCCTGCGTACCCCGACCCATTACGCCGAAGAAATCAAGATCCGCTACGCCTGTGCCCTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCa < 1:727296/150‑1 (MQ=255) cAACGACATCGCCATGGCCCTGCGTACCCCGACCCAGTACGCCGAAGAAATCAAGATCCGCTACGCCTGTGCCCTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTcgcg > 1:1188997/1‑150 (MQ=255) aCGACATCGCCATGGCCCTGCGTACCCCGACCCAGTACGCCGAAGAAATCAAGATCCGCTACGCCTGTGCCCTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑cgcgc < 2:519039/137‑1 (MQ=255) aCGACATCGCCATGGCCCTGCGTACCCCGACCCAGTACGCCGAAGAAATCAAGATCCGCTACGCCTGTGCCCTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑cgcgc > 1:519039/1‑137 (MQ=255) aCGACATCGCCATGGCCCTGCGTACCCCGACCCAGTACGCCGAAGAAATCAAGATCCGCTACGCCTGTGCCCTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGcc > 2:1249808/1‑150 (MQ=255) tACCCCGACCCAGTACGCCGAAGAAATCAAGATCCGCTACGCCTGTGCCCTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGa < 2:1188997/150‑1 (MQ=255) ccccGACCCAGTACGCCGAAGAAATCAAGATCCGCTACGCCTGTGCCCTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGc < 2:1045563/150‑1 (MQ=255) cccGACCCAGTACGCCGAAGAAATCAAGATCCGCTACGCCTGTGCCCTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGcc > 1:974842/1‑150 (MQ=255) ccGACCCAGTACGCCGAAGAAATCAAGATCCGCTACGCCTGTGCCCTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCa > 2:1172119/1‑150 (MQ=255) ccGACCCAGTACGCCGAAGAAATCAAGATCCGCTACGCCTGTGCCCTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGAACGCCCACACCCGCGACCAG‑CTGGCCGGGCGCTTGGCGAGGGGGTGGAgaaa > 2:977825/1‑147 (MQ=255) ccAGTACGCCGAAGAAATCAAGATCCGCTACGCCTGTGCACTGGCAACACTTGTTCGCGCTGGCGAGACCAGCAAGGTGCCGAGAGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTa > 1:989178/1‑150 (MQ=255) gCCGAAGAAATCAAGATCCGCTACGCCTGTGCCCTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGACGAg < 2:974842/150‑1 (MQ=255) gaagaaATCAAGATCCGCTACGCCTGTGCCCTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGACGAGctc > 1:290673/1‑150 (MQ=255) gaagaaATCAAGATCCGCTACGCCTGTGCCCTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCACCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGACGAGctc > 1:1040140/1‑150 (MQ=255) gaagaaATCAAGATCCGCTACGCCTGTGCCCTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCACCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGACGAGctc > 1:1040518/1‑150 (MQ=255) gaaATCAAGATCCGCTACGCCTGTGCCCTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCACCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGACGAGCTCTTc < 2:1040140/150‑1 (MQ=255) gaaATCAAGATCCGCTACGCCTGTGCCCTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCACCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGACGAGCTCTTc < 2:1040518/150‑1 (MQ=255) aaaTCAAGATCCGCTACGCCTGTGCCCTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGACGAGCTCTTCa < 1:1172119/150‑1 (MQ=255) tCAAGATCCGCTACGCCTGTGCCCTGGCCAAAATTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGACGAGCTCTTCAccc < 2:418011/150‑1 (MQ=255) aaGATCCGCTACGCCTGTGCCCTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGACGAGCTCTTCACCCtt > 2:122767/1‑150 (MQ=255) aTCCGCTACGCCTGTGCCCTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGACGAGCTCTTCACCCTTATc < 1:132252/150‑1 (MQ=255) tCCGCTACGCCTGTGCCCTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTgg < 1:1212413/105‑1 (MQ=255) tCCGCTACGCCTGTGCCCTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTgg > 2:1212413/1‑105 (MQ=255) cTACGCCTGTGCCCTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGACGAGCTCTTCACCCTTATCCAGGc < 1:128573/150‑1 (MQ=255) cTACGCCTGTGCCCTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGGGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGACGAGCTCTTCACCCTTATCCAGGc > 2:815589/1‑150 (MQ=255) cGCCTGTGCCCTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGACGAGCTCTTCACCCTTATCCAGGCCGa > 1:587831/1‑150 (MQ=255) gCCTGTGCCCTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGAGCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCGTGGCCGAGGTGGTGGAGCCACGTTACGACGAGCTCTTCACCCGTATCCATGCCGaa < 1:354465/150‑1 (MQ=255) cTGTGCCCTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGACGAGCTCTTCACCCTTATCCAGGCCGAACt > 2:1255582/1‑150 (MQ=255) ccTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGACGAGCTCTTCACCCTTATCCAGGCCGAACTGcgccg > 1:778685/1‑150 (MQ=255) cTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGACGAGCTCTTCACCCTTATCCAGGCCGAACTGcgccgc > 1:433923/1‑150 (MQ=255) cAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGACGAGCTCTTCACCCTTATCCAGGCCGAACTGCGCCGCAGTgg > 2:624220/1‑150 (MQ=255) aCTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGACGAGCTCTTCACCCTTATCCAGGCCGAACTGCGCCGCAGTGGCTa > 1:1032668/1‑150 (MQ=255) gctggcgctggcGAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGACGAGCTCTTCACCCTTATCCAGGCCGAACTGCGCCGCAGTGGCTACGaa < 2:433923/150‑1 (MQ=255) gctggcgctggcGAGACCATCAAGGTGCCGAGCGAGGCCGATGACTGGA‑GTTCCGAGGTGTCGTGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGACGAGCTCTTCACCCTTATCCAGGCCGAACTGCGCCGCAGTGGCTACGaa < 1:1249808/150‑1 (MQ=255) tggcgctggcgAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGACGAGCTCTTCACCCTTATCCAGGCCGAACTGCGCCGCAGTGGCTACGAAGa > 2:167837/1‑150 (MQ=255) tggcgctggcgAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGACGAGCTCTTCACCCTTATCCAGGCCGAACTGCGCCGCAGTGGCTACGAAGa > 2:40714/1‑150 (MQ=255) ggcgctggcgAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTCGTGGAGCCACGTTCCCCCGGTCTCGTCACCCTTATCCAGGCCGAACTGCGCCGCAGTGGCTACGAAGAc < 1:482401/150‑1 (MQ=255) ggcgctggcgAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCCGTTTTGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGACGAGCTCTTCACCCTTATCCAGGCCGAACTGCGCCGCAGTGGCTATGAAGAc < 1:656048/150‑1 (MQ=255) gctggcgAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGACGAGCTCTTCACCCTTATCCAGGCCGAACTGCGCCGCAGTGGCTACGAAGACCTg < 1:349640/150‑1 (MQ=255) ctggcgAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGACGAGCTCTTCACCCTTATCCAGGCCGAACTGCGCCGCAGTGGCTACGAAGACCTgg < 1:40714/150‑1 (MQ=255) tCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGAGGACCATTTCACCCTTATCCATGCCGAAATGCGCCGCAGTGGCTACGAAGGCCTGGTGCCCGCCGGCa > 1:22770/1‑150 (MQ=255) gTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGACGAGCTCTTCACCCTTATCCAGGCCGAACTGCGCCGCAGTGGCTACGAAGACCTGGTGCCCGCCGGCATCGTCCTCACcggc < 2:989178/150‑1 (MQ=255) cGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGCCCCTGGCCCAGGTGGTGGAGCCACGTTACGACGAGCTCTTCACCCTTATCCAGGCCGAACTGCGCCGCAGTGGCTACGAAGACCTGGTGCCCGCCGGCATCGTCCTCACCGGCGGCAc < 1:977825/150‑1 (MQ=255) aTCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGACGAGCTCTTCACCCTTATCCAGGCCGAACTGCGCCGCAGTGGCTACGAAGACCTGGTGCCCGCCGGCATCGTCCTCACCGGCGGCACcg > 1:29980/1‑150 (MQ=255) | GCTGGCGACCAGGTGACCAACGACATCGCCATGGCCCTGCGTACCCCGACCCAGTACGCCGAAGAAATCAAGATCCGCTACGCCTGTGCCCTGGCCAAACTTGCTGGCGCTGGCGAGACCATCAAGGTGCCGAGCGTGGGCGATCGCCCAC‑CGCGCGAGCTGTCGCGCCAGGCCCTGGCCGAGGTGGTGGAGCCACGTTACGACGAGCTCTTCACCCTTATCCAGGCCGAACTGCGCCGCAGTGGCTACGAAGACCTGGTGCCCGCCGGCATCGTCCTCACCGGCGGCACCG > NC_002947/1528404‑1528695 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |