Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,544,534 G→C 14.3% intergenic (+51/+63) mdfA → / ← ampG multidrug MFS transporter/muropeptide permease AmpG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,544,5340GC14.3% 129.9 / 15.1 49intergenic (+51/+63)mdfA/ampGmultidrug MFS transporter/muropeptide permease AmpG
Reads supporting (aligned to +/- strand):  ref base G (22/20);  new base C (3/4);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 7.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.17e-01

CATTTTGCCCTGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGATC  >  NC_002947/1544385‑1544681
                                                                                                                                                     |                                                                                                                                                   
cATTTTGCCCTGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAgg                                                                                                                                                     >  2:97920/1‑150 (MQ=255)
       cccTGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGgccgcgc                                                                                                                                              <  1:97920/150‑1 (MQ=255)
        ccTGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGgccgcgcc                                                                                                                                             >  1:4914/1‑150 (MQ=255)
             gggcggGGGTGGCGGGGGGGTTGGCGCTGTGGCCGCTGTGGTTTGAGGTCTGGTGTCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCttt                                                                                                                                        <  2:34062/149‑1 (MQ=39)
             cggcggGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGAGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCttt                                                                                                                                        <  1:476952/150‑1 (MQ=255)
                 ggggggggCGGGGGCGTGGGCGCGGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAg                                                                                                                                    <  2:288131/150‑1 (MQ=255)
                     gTGGGGGGGGCGTCGGCGCTGAGGCCGCGGTGGTTTGTGTTGGGTGGGGGGCGTGGGGAAGGGGTTGTAGCTCGATACTCTGTATTGCCGGGGCCTGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGc                                                                                                                                <  2:1232870/150‑1 (MQ=38)
                       ggCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCgg                                                                                                                              <  1:330153/150‑1 (MQ=25)
                        gCGGGGGCGTGGGCGCTGTGGCCGCGGTGGTTTGTGGTGGGTGGGGGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCggg                                                                                                                             <  2:370315/150‑1 (MQ=25)
                              gCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTCGCAGCGGGTTTAcc                                                                                                                       >  1:466766/1‑150 (MQ=255)
                                    gagcTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGaa                                                                                                                 >  2:32068/3‑150 (MQ=255)
                                     cgcTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGgcacgc                                                                                                                                               <  1:55390/119‑5 (MQ=255)
                                     cgcTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGgcacgc                                                                                                                                               >  2:55390/1‑115 (MQ=255)
                                      gcTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAaga                                                                                                               >  1:1201845/1‑150 (MQ=255)
                                       tgggggCCGGTGGGGTTGGTGGTGGGGGGGCGGCCGGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAagag                                                                                                              <  2:909740/146‑1 (MQ=37)
                                       cTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAagag                                                                                                              <  2:498389/150‑1 (MQ=255)
                                          tgGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCATTGTAGGAGCGGGTTTACACGCGAAGAGGcc                                                                                                           >  1:540212/1‑150 (MQ=255)
                                                     gTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCaa                                                                                                <  1:32068/150‑1 (MQ=255)
                                                     gTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCaa                                                                                                <  1:842614/150‑1 (MQ=255)
                                                          tggtggGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACaa                                                                                           >  1:805965/1‑150 (MQ=255)
                                                                                    gCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAg                                                                 >  1:698619/1‑150 (MQ=255)
                                                                                         aGCTCGATACTCGGTATTCCCGGGGCCCGCTTCTTCGCGGGTAAACACGCTTCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAACAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGgtc                                                            <  2:277988/150‑1 (MQ=37)
                                                                                            tCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGc                                                                                                            >  2:968121/1‑99 (MQ=255)
                                                                                            tCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGc                                                                                                            <  1:968121/99‑1 (MQ=255)
                                                                                             ggATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCGGCTCTTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTcc                                                        <  2:941459/149‑1 (MQ=255)
                                                                                                            ccGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTcccc                                         <  2:466766/150‑1 (MQ=255)
                                                                                                             cGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGc                                                                                                            >  1:910814/1‑82 (MQ=255)
                                                                                                             cGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGc                                                                                                            <  2:910814/82‑1 (MQ=255)
                                                                                                                    ggccTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGc                                 >  1:520885/1‑150 (MQ=255)
                                                                                                                       ctctTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAgg                                                                                                   >  1:1002908/1‑81 (MQ=255)
                                                                                                                       ctctTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAgg                                                                                                   <  2:1002908/81‑1 (MQ=255)
                                                                                                                          ttCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGCAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCTCTCAGCCAGCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTg                           >  1:835821/1‑150 (MQ=255)
                                                                                                                            cgcgGGTAAACCCGCTGCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGAt                         <  2:640292/150‑1 (MQ=255)
                                                                                                                            cgcgGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGAt                         <  2:4914/150‑1 (MQ=255)
                                                                                                                                 gTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCAc                    >  1:1103850/1‑150 (MQ=255)
                                                                                                                                     aCCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGa                          <  1:539095/140‑1 (MQ=255)
                                                                                                                                     aCCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGa                          >  2:539095/1‑140 (MQ=255)
                                                                                                                                      cccGCTCCTACCCAGGGCCGCGCCGCTTTGTAGGAGCGGTTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTAAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAg               >  1:540733/1‑150 (MQ=255)
                                                                                                                                       ccGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCTCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAgg              >  2:82941/1‑150 (MQ=255)
                                                                                                                                       ccGCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGc                       <  2:346386/141‑1 (MQ=25)
                                                                                                                                       ccGCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGc                       >  1:346386/1‑141 (MQ=25)
                                                                                                                                       ccGCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCa                     <  2:1093966/143‑1 (MQ=25)
                                                                                                                                       ccGCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCa                     >  1:1093966/1‑143 (MQ=25)
                                                                                                                                       ccGCTCCTACAAAGCGGCGCGGCCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAgg              >  2:209746/1‑150 (MQ=25)
                                                                                                                                         gCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCATGGCCGCTCAGCCACCGAGTgg                                                              >  2:781567/1‑100 (MQ=255)
                                                                                                                                         gCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCATGGCCGCTCAGCCACCGAGTgg                                                              <  1:781567/100‑1 (MQ=255)
                                                                                                                                         gCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGc            >  2:430186/1‑150 (MQ=255)
                                                                                                                                              tACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGCGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatca       >  2:329096/1‑150 (MQ=255)
                                                                                                                                               acacAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatcag      >  1:777660/1‑150 (MQ=255)
                                                                                                                                                   aGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCgatcaggatc  >  2:725331/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CATTTTGCCCTGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCCGCTTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGCAATACAAAGCTTCAGGGCCGCTCAGCCACCGAGTGGTCGTCCGCCACCGGCGTCCCCCGCCCGGCTTCCTGATGCCACTGCAGGGCGATCAGGATC  >  NC_002947/1544385‑1544681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: