| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 35,012 | C→T | 6.7% | intergenic (+30/+28) | czrSA → / ← PP_0031 | metal‑sensor histidine kinase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 35,012 | 0 | C | T | 6.7% | 168.1 / 2.7 | 60 | intergenic (+30/+28) | czrSA/PP_0031 | metal‑sensor histidine kinase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (30/26); new base T (2/2); total (32/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.23e-01 | |||||||||||
TCGATCATGAGTCTGCACCAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGGTGTGAGAAGTACTGAGCATTCA > NC_002947/34863‑35152 | tCGATCATGAGTCTGCCCCAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCACTc < 1:1009817/150‑1 (MQ=255) gATCATGAGTCTGCACCAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGa > 2:528001/1‑150 (MQ=255) tCATGAGTCTGCACCAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACg > 1:385957/1‑150 (MQ=255) tCATGAGTCTGCACCAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCCGCAATAACCGCCGCTTAAATTTCTCACACACGATTAGGCCCGTCAATCGACg < 1:482418/150‑1 (MQ=255) tGAGTCTGCACCAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCGATTGACGGGc < 2:1184539/150‑1 (MQ=255) tgTCTGCACCAGGGATCGGCGAGCGTGGAGGTTACCGAAGAAAACTTTACCTGGTTCCACCTTCTCTTTCCGGAAGCCCAGCAAATTCATCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCt < 1:1179020/149‑1 (MQ=255) gTCTGCACCAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATAGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCtt > 1:1031748/1‑150 (MQ=255) cACCAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTtttgtt < 1:511561/150‑1 (MQ=255) acaaGGGCTCGGCGAACATGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTtttgttt > 2:1043404/4‑150 (MQ=255) cAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGc > 2:335106/1‑150 (MQ=255) cAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGc < 1:528001/150‑1 (MQ=255) aGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGcc > 2:602487/1‑150 (MQ=255) aGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGcc > 2:1167903/1‑150 (MQ=255) aTCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCaa < 1:669263/150‑1 (MQ=255) cGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACca > 2:172793/1‑150 (MQ=255) agaagaaAACTACGCGTGTATCCACCTTCAGTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGTCCTTTTGTTTCCCTCAATGCATCAGTGCCGAAGGACca > 2:79058/2‑150 (MQ=255) gaagaaAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACcac < 1:1167903/150‑1 (MQ=255) aagaaCTCTTCACGTGCTTGCGCATTCCCTTACCGGAATCCCAGCAAATGCAGCCGGCAATGAGCGCCGGTTAAATTTCGCCTACACGATTGGGGGCGTCAATCGAAAGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACcaca < 2:115483/150‑1 (MQ=255) aagaaAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACcacc > 2:1191545/1‑149 (MQ=255) gaaAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAgg < 2:385957/150‑1 (MQ=255) aaaCTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGt > 1:37935/1‑150 (MQ=255) cTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCa > 1:1198700/1‑150 (MQ=255) cACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAgg < 2:1181170/142‑1 (MQ=255) cACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAgg > 1:1181170/1‑142 (MQ=255) cACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATgg < 1:172793/150‑1 (MQ=255) cACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAAGGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGCCGGGCCTTTTGTTTGCTTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATgg < 2:62777/150‑1 (MQ=255) ccTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGc < 2:1198700/150‑1 (MQ=255) cTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCg > 1:1110587/1‑150 (MQ=255) ccACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCa < 2:611173/150‑1 (MQ=255) tttCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCtttt < 2:899078/87‑1 (MQ=255) tttCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCtttt > 1:899078/1‑87 (MQ=255) gAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTtaa < 1:1005034/149‑1 (MQ=255) gAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTtaa > 2:1005034/1‑149 (MQ=255) ccAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAAtct > 2:751411/1‑150 (MQ=255) gCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAAtcttct < 1:150612/150‑1 (MQ=255) aGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGCACAACGCTTCCATTGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCa > 2:359269/1‑150 (MQ=255) ccGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCa > 1:1247056/1‑81 (MQ=255) ccGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCa < 2:1247056/81‑1 (MQ=255) aaTGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTgg > 2:168095/1‑150 (MQ=255) aTGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTATTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGc > 1:1141560/1‑150 (MQ=255) ccgccgCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGttt > 2:553626/1‑150 (MQ=255) gccgcTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGttttt > 1:1152015/1‑150 (MQ=255) ttGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGTACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGgtgt < 1:602487/150‑1 (MQ=255) ttGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGgtgt < 1:335106/150‑1 (MQ=255) cAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAAAGTTCCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGGTGTGAGaa < 1:79058/150‑1 (MQ=255) cGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGGTGTGAGAAGTACTg < 2:37935/150‑1 (MQ=255) acaaaaGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGGTGTGAGAAGTACTg > 2:250718/6‑150 (MQ=255) gATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGGTGTGAGAAGTACTGa > 1:817492/1‑150 (MQ=255) aaaGGCCCGTCGATTGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCa < 1:641168/110‑1 (MQ=255) aaaGGCCCGTCGATTGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCa > 2:641168/3‑112 (MQ=255) ggCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAAtctt > 2:495789/1‑99 (MQ=255) ggCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAAtctt < 1:495789/99‑1 (MQ=255) ggCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGGTGTGAGAAGTACTGAGCAtt < 2:817492/150‑1 (MQ=255) gCCCGTCAATCGACGGGCCTTTTGTTTGCTTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGttt < 1:694450/120‑1 (MQ=255) gCCCGTCAATCGACGGGCCTTTTGTTTGCTTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGttt > 2:694450/1‑120 (MQ=255) gCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGGTGTGAGAAGTACTGAGCATTc > 2:41304/1‑150 (MQ=255) cccGTCGATTGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGGTGTGAGAAGTACTGAGCATTCa > 1:713123/1‑150 (MQ=255) gTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGgt < 2:384048/126‑1 (MQ=255) gTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGgt > 1:384048/1‑126 (MQ=255) gTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGGTGTGAGAAGt > 1:1046521/1‑135 (MQ=255) gTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGGTGTGAGAAGt < 2:1046521/135‑1 (MQ=255) | TCGATCATGAGTCTGCACCAGGGATCGGCGAGCGTGGAGGTGACCGAAGAAAACTTCACCTGGTTCCACCTTCACTTTCCGGAAGCCCAGCAAATGCAGCCGGCAATGACCGCCGCTTAAATTGCGCAGACACGATTAGGCCCGTCAATCGACGGGCCTTTTGTTTGCCTCAATGCATCAGTGCCGAAGGACCACAGGGTCCATGGGCGACCGCAGAATCACCGACTTAATAATCTTCTGTTCGTCAGGATTGGCGTTTTTCCACAGGTGTGAGAAGTACTGAGCATTCA > NC_002947/34863‑35152 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |