Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,732,715 C→A 7.1% E399D (GAG→GAT)  PP_1526 ← beta‑(1‑3)‑glucosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,732,7150CA 7.1% 174.1 / 2.0 56E399D (GAG→GAT) PP_1526beta‑(1‑3)‑glucosyl transferase
Reads supporting (aligned to +/- strand):  ref base C (25/27);  new base A (2/2);  total (27/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-01

GTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGCCGAGGGCCAGCAGCACGCCCACGGTCAGGCTGAACCAGGTGCTGTATTGCTGGCTGTAGTCGTAGGCGATCCACACCAGCACCGACCCGCACAGGA  >  NC_002947/1732568‑1732863
                                                                                                                                                   |                                                                                                                                                    
gTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGACTc                                                                                                                                                    <  1:1017926/150‑1 (MQ=255)
 tCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCg                                                                                                                                                   >  2:850193/1‑150 (MQ=255)
           ccAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGt                                                                                                                                         <  1:850193/150‑1 (MQ=255)
            cAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTg                                                                                                                                        <  1:1194048/150‑1 (MQ=255)
                  gTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTc                                                                                                                                  >  1:514859/1‑150 (MQ=255)
                  gTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTc                                                                                                                                  >  2:289781/1‑150 (MQ=255)
                   tCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCGGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCg                                                                                                                                 >  2:1008572/1‑150 (MQ=255)
                    ccAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCGCGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCgg                                                                                                                                <  2:1279685/150‑1 (MQ=255)
                     cAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGCGCCTCCGt                                                                                                                               <  2:959695/150‑1 (MQ=255)
                       gggTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCTGTCATTCCTGGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGa                                                                                                                             <  2:359326/150‑1 (MQ=255)
                          tCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGagca                                                                                                                          <  1:729491/150‑1 (MQ=255)
                              tttCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCAc                                                                                                                      >  2:477566/1‑150 (MQ=255)
                                 cACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGt                                                                                                                               >  2:54785/1‑138 (MQ=255)
                                 cACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGt                                                                                                                               <  1:54785/138‑1 (MQ=255)
                                    cATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGaa                                                                                                                <  2:420333/150‑1 (MQ=255)
                                      tctcAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAaca                                                                                                              <  1:289781/150‑1 (MQ=255)
                                      tctcAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAaca                                                                                                              >  1:1094291/1‑150 (MQ=255)
                                              ggCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATg                                                                                                                  >  1:237863/1‑138 (MQ=255)
                                              ggCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATg                                                                                                                  <  2:237863/138‑1 (MQ=255)
                                              ggCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAgc                                                                                                      <  1:420986/150‑1 (MQ=255)
                                               gCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAgcg                                                                                                     >  2:1123010/1‑150 (MQ=255)
                                                cTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAgcgc                                                                                                    >  1:1169263/1‑150 (MQ=255)
                                                              gCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTgg                                                                                                                                       <  1:1229394/101‑1 (MQ=255)
                                                              gCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTgg                                                                                                                                       >  2:1229394/1‑101 (MQ=255)
                                                                  aTGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGCCGAGGGCCAGCAGCAc                                                                                  >  2:189741/1‑150 (MQ=255)
                                                                            acCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGCCGAGGGCCAGCAGCACGCCCACGGTc                                                                        >  1:1016563/1‑150 (MQ=255)
                                                                              cTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGCCGAGGGCCAGCAGCACGCCCACGGTCAg                                                                      >  2:821098/1‑150 (MQ=255)
                                                                              cTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCATCGGCCAGTTCGTGGGCCTCGGt                                                                                                                               <  2:434945/93‑1 (MQ=255)
                                                                              cTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCATCGGCCAGTTCGTGGGCCTCGGt                                                                                                                               <  2:434750/93‑1 (MQ=255)
                                                                              cTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCATCGGCCAGTTCGTGGGCCTCGGt                                                                                                                               >  1:434750/1‑93 (MQ=255)
                                                                              cTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCATCGGCCAGTTCGTGGGCCTCGGt                                                                                                                               >  1:434945/1‑93 (MQ=255)
                                                                                tGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGCCGAGGGCCAGCAGCACGCCCACGGTCAGGc                                                                    <  2:644312/150‑1 (MQ=255)
                                                                                tGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGCCGAGGGCCAGCAGCACGCCCACGGTCAGGc                                                                    <  2:514859/150‑1 (MQ=255)
                                                                                tGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGCCGAGGGCCAGAAGCACGCCCACGGTCAGGc                                                                    <  1:1008572/150‑1 (MQ=255)
                                                                                  ggCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGCCGAGGGCCAGCAGCACGCCCACGGTCAGGCTg                                                                  >  2:1106657/1‑150 (MQ=255)
                                                                                       gTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGCCGAGGGCCAGCAGCACGCCCACGGTCAGGCTGAACCa                                                             >  1:95781/1‑150 (MQ=255)
                                                                                                    gCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGCCGAGGGCCAGCAGCACGCCCACGGTCAGGCTGAACCAGGTGCTGTATTgc                                                <  1:923792/150‑1 (MQ=255)
                                                                                                    gCCTGCACGGGAAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGCCGAGGGCCAGCAGCACGCCCACGGTCAGGCTGAACCAGGTGCTGTATTgc                                                >  2:1277639/1‑150 (MQ=255)
                                                                                                     ccTGCACGGGCAGGAACTCGGGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGCCGAGGGCCAGCAGCACGCCCACGGTCAGGCTGAACCAGGTGCTGTATTgct                                               <  2:95781/150‑1 (MQ=255)
                                                                                                         cACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGCCGAGGGCCAGCAGCACGCCCACGGTAAGGCTGAACCAGGTGCTGTATTgctggct                                           <  2:959738/150‑1 (MQ=255)
                                                                                                              gCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGCCGAGGGCCAGCAGCACGCCCACGGTCAGGCTGAACCAGGTGCTGTATTGCTGGCTGTAGt                                      >  2:480232/1‑150 (MQ=255)
                                                                                                              gCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGCCGAGGGCCAGCAGCACGCCCACGGTCAGGCTGAACCAGGTGCTGTATTGCTGGCTGTAGt                                      >  1:1065232/1‑150 (MQ=255)
                                                                                                                aGGAAATCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGCCGAGGGCCAGCAGCACGCCCACGGTCAGGCTGAACCAGGTGCTGTATTGCTGGCTGTAGTCg                                    <  1:1277639/150‑1 (MQ=255)
                                                                                                                   agcTCGCGGCGGCGCTTGTGTATGCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGGAGCACGATGAACACACCCAGCGCGCCGAGGGCCAGCAGCACGCCCACGGTCAGGCTGAACCAGGTGCTGTATTGCTGGCTGTAGTCGTAg                                 <  1:852418/148‑1 (MQ=255)
                                                                                                                      tCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGcc                                                                                                 <  1:742190/83‑1 (MQ=255)
                                                                                                                      tCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGcc                                                                                                 >  2:742190/1‑83 (MQ=255)
                                                                                                                      tCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGCCGAGGGCCAGCAGCACGCCCACGGTCAGGCTGAACCAGGTGCTGTATTgctggctg                                          >  1:252846/1‑138 (MQ=255)
                                                                                                                      tCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGCCGAGGGCCAGCAGCACGCCCACGGTCAGGCTGAACCAGGTGCTGTATTgctggctg                                          <  2:252846/138‑1 (MQ=255)
                                                                                                                      tCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGCCGAGGGCCAGCAGCACGCCCACGGTCAGGCTGAACCAGGTGCTGTATTGCTGGCTGTAGTCGTAGGCg                              >  2:531748/1‑150 (MQ=255)
                                                                                                                       cgcgGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGCCGAGGGCCAGCAGCACGCCCACGGTCAGGCTGAACCAGGTGCTGTATTGCTGGCTGTAGTCGTAGGCGa                             <  1:531748/150‑1 (MQ=255)
                                                                                                                        gcggcggcgCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGCCGAGGGCCAGCAGCACGCCCACGGTCAGGCTGAACCAGGTGCTGTATTGCTGGCTGTAGTCGTAGGCGAt                            <  1:1123010/150‑1 (MQ=255)
                                                                                                                                gcTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGCCGAGGGCCAGCAGCACGCCCACGGTCAGGCTGAACCAGGTGCTGTATTGCTGGCTGTAGTCGTAGGCGATCCACACCa                    >  2:344582/1‑150 (MQ=255)
                                                                                                                                             gACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGCCGAGGGCCAGCAGCACGCCCACGGTCAGGCTGAACCAGGTGCTGTATTGCTGGCTGTAGTCGTAGGCGATCca                         >  2:716786/1‑132 (MQ=255)
                                                                                                                                             gACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGCCGAGGGCCAGCAGCACGCCCACGGTCAGGCTGAACCAGGTGCTGTATTGCTGGCTGTAGTCGTAGGCGATCca                         <  1:716786/132‑1 (MQ=255)
                                                                                                                                                 gCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGCCGAGGGCCAGCAGCACGCCCACGGTCAGGCTGAACCAGGTGCTGTATTGCTGGCTGTa                                        >  1:803703/1‑113 (MQ=255)
                                                                                                                                                 gCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGCCGAGGGCCAGCAGCACGCCCACGGTCAGGCTGAACCAGGTGCTGTATTGCTGGCTGTa                                        <  2:803703/113‑1 (MQ=255)
                                                                                                                                                  ccTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGCCGAGGGCCAGCAGCACGCCCACGGTCAGGCTGAACCAGGTGCTGTATTGCTGGCTGTAGTCGTAGGCGATCCACACCAGCACCGACCCGCACAGGa  >  1:759742/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GTCCAGGGCGGCCAGGGCGTCCAGGGTCTGTTTCACCATCTCAGGTGGCTCGTTGTAGCACGGCACATGCACCGACACCTTGGGCCGGTAGGCAGTGTCGGCCTGCACGGGCAGGAACTCGCGGCGGCGCTTGTGTATCCAGACCGCCTCGGCCAGTTCGTGGGCCTCGGTGAGCAGCACGATGAACACACCCAGCGCGCCGAGGGCCAGCAGCACGCCCACGGTCAGGCTGAACCAGGTGCTGTATTGCTGGCTGTAGTCGTAGGCGATCCACACCAGCACCGACCCGCACAGGA  >  NC_002947/1732568‑1732863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑