Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,818,526 A→G 12.3% Y209C (TAC→TGC)  PP_1622 → M23B subfamily metallopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,818,5260AG12.3% 136.1 / 8.0 55Y209C (TAC→TGC) PP_1622M23B subfamily metallopeptidase
Reads supporting (aligned to +/- strand):  ref base A (23/25);  new base G (4/3);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 7.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

TTTGAATAAAGGCATTGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGT  >  NC_002947/1818380‑1818672
                                                                                                                                                  |                                                                                                                                                   
tttGAATAAAGGCATTGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACgg                                                                                                                                                  >  1:552501/1‑150 (MQ=255)
 ttGAATAAAGGCATTGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGt                                                                                                                                                 <  2:861640/150‑1 (MQ=255)
              ttGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTGCGGTTATGACCGTAGGc                                                                                                                                    >  1:1166828/1‑150 (MQ=255)
              ttGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGc                                                                                                                                    >  1:1105182/1‑150 (MQ=255)
               tGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTGCGGTTATGACCGTAGGca                                                                                                                                   <  1:927585/150‑2 (MQ=255)
               tGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTGCGGTTATGACCGTAGGca                                                                                                                                   <  2:1166828/150‑2 (MQ=255)
                  tatCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGtt                                                                                                                                <  2:86092/150‑1 (MQ=255)
                    tCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTgg                                                                                                                              >  2:816425/1‑150 (MQ=255)
                       ccGGTGATTTGGGACAGCCTGTTTTCGCTGCGTATGATGGTGCGGTGGTTTATGCCGGGAGTGGGTTGAGGGGCTACGGCGAGCTGATCATCATCAAGAACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTc                                                                                                                           >  1:943465/1‑150 (MQ=255)
                        cGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCg                                                                                                                          <  2:1185361/150‑1 (MQ=255)
                           tGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTgggtttgta                                                                                                                       <  2:403750/150‑8 (MQ=255)
                           tGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGa                                                                                                                       <  1:254076/150‑1 (MQ=255)
                           tGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGa                                                                                                                       <  2:1119007/150‑1 (MQ=255)
                            gATTTGGGTCAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATATCCGCAGGCTGTTGGTTCGGGA‑g                                                                                                                     <  2:124/150‑1 (MQ=255)
                              tttGGGACAGCCTGTTTTCGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑ggg                                                                                                                   <  1:888282/150‑1 (MQ=255)
                                    aCAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGgcagca                                                                                                             >  1:572989/1‑150 (MQ=255)
                                         cTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑gg                                                                                                                    <  1:1213999/138‑1 (MQ=255)
                                         cTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑gg                                                                                                                    >  2:1213999/1‑138 (MQ=255)
                                                   tgcGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAggcagggca                                                                                              >  1:1004235/1‑150 (MQ=255)
                                                   tgcGTCTGATGGTGCGGCGGTTTATGCCGGGAGTGGCTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAggcagggca                                                                                              >  2:197780/1‑150 (MQ=255)
                                                       tCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCg                                                                                          >  1:559673/1‑150 (MQ=255)
                                                               tGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTg                                                                                                                               <  2:478937/106‑1 (MQ=255)
                                                               tGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTg                                                                                                                               >  1:478937/1‑106 (MQ=255)
                                                                 cGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCCTCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAgg                                                                                                           >  2:5316/1‑123 (MQ=255)
                                                                 cGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAgg                                                                                                           <  1:5316/123‑1 (MQ=255)
                                                                  ggtggtTTATGCCGGGAGTGGTTTGAGGGGCTCCGGAGAGCGGATCATAATCAAGCACAGGGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAAt                                                                               <  2:943465/150‑1 (MQ=255)
                                                                      gtTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATgggg                                                                           <  1:735900/150‑1 (MQ=255)
                                                                              cGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACggg                                                                   <  2:552501/150‑1 (MQ=255)
                                                                                   gTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACg                                                                     >  2:890412/1‑143 (MQ=255)
                                                                                   gTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACg                                                                     <  1:890412/143‑1 (MQ=255)
                                                                                       tttGAGGGGCTACGGCGAGCTGATCATCATCACGCACCGCAACACCTTCGGCAGTGCCTGCGGTCATAACTGCAGCACGGTCG‑CCGACATTTCGTTGCTGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACGAATCg                                                          >  1:292489/1‑150 (MQ=255)
                                                                                          gAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGt                                                       <  2:1004235/150‑1 (MQ=255)
                                                                                             gggCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGaa                                                    <  1:816425/150‑1 (MQ=255)
                                                                                                                atcatcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTcgc                                 >  1:701807/1‑150 (MQ=255)
                                                                                                                   atcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTcgccgc                              >  1:1021367/1‑150 (MQ=255)
                                                                                                                   atcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTcgccgc                              <  2:559673/150‑1 (MQ=255)
                                                                                                                   atcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTc                                   >  1:233087/1‑145 (MQ=255)
                                                                                                                   atcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTc                                   <  2:233087/145‑1 (MQ=255)
                                                                                                                    tcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCgccgcc                             >  1:66751/1‑150 (MQ=255)
                                                                                                                            aGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGATCGTTGGTTG‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACTGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCaaa                     >  1:234705/1‑150 (MQ=255)
                                                                                                                                acacCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCg                 <  2:292489/150‑1 (MQ=255)
                                                                                                                                 cacCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGt                <  2:66751/150‑1 (MQ=255)
                                                                                                                                    cTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGa             <  2:572989/150‑1 (MQ=255)
                                                                                                                                    cTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAAGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTTGAGATTCGCCGCTAGGGCAAACCGGTcca             >  2:7102/1‑148 (MQ=255)
                                                                                                                                     tACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGcac                                                                >  1:1280855/1‑98 (MQ=255)
                                                                                                                                     tACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGcac                                                                <  2:1280855/98‑1 (MQ=255)
                                                                                                                                     tACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGAt            >  1:1169835/1‑150 (MQ=255)
                                                                                                                                     tACGTCAGTGACTACGGTCAAAAACGCAGGCTGTTGGTTCGGTA‑GGGGTAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGAt            <  2:1105182/150‑1 (MQ=255)
                                                                                                                                          aacagCCTGCGGTTATGACCGTAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACt       >  1:58590/5‑150 (MQ=255)
                                                                                                                                             tGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGt                                                                          <  1:148984/80‑1 (MQ=255)
                                                                                                                                             tGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGt                                                                          >  2:148984/1‑80 (MQ=255)
                                                                                                                                             agCCTGCGGTTATGACCGTAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCa    <  1:1238724/149‑1 (MQ=255)
                                                                                                                                               ccTGCGGTTATGACCGTAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGt  >  1:555032/1‑150 (MQ=255)
                                                                                                                                               ccTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGt  >  1:570055/1‑150 (MQ=255)
                                                                                                                                               ccTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGt  >  1:736163/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TTTGAATAAAGGCATTGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGT  >  NC_002947/1818380‑1818672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑