Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,818,531 C→T 10.7% H211Y (CAT→TAT)  PP_1622 → M23B subfamily metallopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,818,5310CT10.7% 138.9 / 8.0 56H211Y (CAT→TAT) PP_1622M23B subfamily metallopeptidase
Reads supporting (aligned to +/- strand):  ref base C (25/25);  new base T (3/3);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.85e-01

TTGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGTTCCTGC  >  NC_002947/1818394‑1818678
                                                                                                                                         |                                                                                                                                                    
ttGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTGCGGTTATGACCGTAGGc                                                                                                                                          >  1:1166828/1‑150 (MQ=255)
ttGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGc                                                                                                                                          >  1:1105182/1‑150 (MQ=255)
 tGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTGCGGTTATGACCGTAGGca                                                                                                                                         <  1:927585/150‑2 (MQ=255)
 tGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTGCGGTTATGACCGTAGGca                                                                                                                                         <  2:1166828/150‑2 (MQ=255)
    tatCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGtt                                                                                                                                      <  2:86092/150‑1 (MQ=255)
      tCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTgg                                                                                                                                    >  2:816425/1‑150 (MQ=255)
         ccGGTGATTTGGGACAGCCTGTTTTCGCTGCGTATGATGGTGCGGTGGTTTATGCCGGGAGTGGGTTGAGGGGCTACGGCGAGCTGATCATCATCAAGAACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTc                                                                                                                                 >  1:943465/1‑150 (MQ=255)
          cGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCg                                                                                                                                <  2:1185361/150‑1 (MQ=255)
             tGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTgggtttgta                                                                                                                             <  2:403750/150‑8 (MQ=255)
             tGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGa                                                                                                                             <  2:1119007/150‑1 (MQ=255)
             tGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGa                                                                                                                             <  1:254076/150‑1 (MQ=255)
              gATTTGGGTCAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATATCCGCAGGCTGTTGGTTCGGGA‑g                                                                                                                           <  2:124/150‑1 (MQ=255)
                tttGGGACAGCCTGTTTTCGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑ggg                                                                                                                         <  1:888282/150‑1 (MQ=255)
                      aCAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGgcagca                                                                                                                   >  1:572989/1‑150 (MQ=255)
                           cTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑gg                                                                                                                          >  2:1213999/1‑138 (MQ=255)
                           cTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑gg                                                                                                                          <  1:1213999/138‑1 (MQ=255)
                                     tgcGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAggcagggca                                                                                                    >  1:1004235/1‑150 (MQ=255)
                                     tgcGTCTGATGGTGCGGCGGTTTATGCCGGGAGTGGCTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAggcagggca                                                                                                    >  2:197780/1‑150 (MQ=255)
                                         tCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCg                                                                                                >  1:559673/1‑150 (MQ=255)
                                                 tGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTg                                                                                                                                     >  1:478937/1‑106 (MQ=255)
                                                 tGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTg                                                                                                                                     <  2:478937/106‑1 (MQ=255)
                                                   cGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCCTCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAgg                                                                                                                 >  2:5316/1‑123 (MQ=255)
                                                   cGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAgg                                                                                                                 <  1:5316/123‑1 (MQ=255)
                                                    ggtggtTTATGCCGGGAGTGGTTTGAGGGGCTCCGGAGAGCGGATCATAATCAAGCACAGGGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAAt                                                                                     <  2:943465/150‑1 (MQ=255)
                                                        gtTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATgggg                                                                                 <  1:735900/150‑1 (MQ=255)
                                                                cGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACggg                                                                         <  2:552501/150‑1 (MQ=255)
                                                                     gTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACg                                                                           <  1:890412/143‑1 (MQ=255)
                                                                     gTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACg                                                                           >  2:890412/1‑143 (MQ=255)
                                                                         tttGAGGGGCTACGGCGAGCTGATCATCATCACGCACCGCAACACCTTCGGCAGTGCCTGCGGTCATAACTGCAGCACGGTCG‑CCGACATTTCGTTGCTGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACGAATCg                                                                >  1:292489/1‑150 (MQ=255)
                                                                            gAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGt                                                             <  2:1004235/150‑1 (MQ=255)
                                                                               gggCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGaa                                                          <  1:816425/150‑1 (MQ=255)
                                                                                                  atcatcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTcgc                                       >  1:701807/1‑150 (MQ=255)
                                                                                                     atcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTcgccgc                                    >  1:1021367/1‑150 (MQ=255)
                                                                                                     atcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTcgccgc                                    <  2:559673/150‑1 (MQ=255)
                                                                                                     atcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTc                                         <  2:233087/145‑1 (MQ=255)
                                                                                                     atcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTc                                         >  1:233087/1‑145 (MQ=255)
                                                                                                      tcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCgccgcc                                   >  1:66751/1‑150 (MQ=255)
                                                                                                              aGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGATCGTTGGTTG‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACTGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCaaa                           >  1:234705/1‑150 (MQ=255)
                                                                                                                  acacCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCg                       <  2:292489/150‑1 (MQ=255)
                                                                                                                   cacCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGt                      <  2:66751/150‑1 (MQ=255)
                                                                                                                      cTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGa                   <  2:572989/150‑1 (MQ=255)
                                                                                                                      cTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAAGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTTGAGATTCGCCGCTAGGGCAAACCGGTcca                   >  2:7102/1‑148 (MQ=255)
                                                                                                                       tACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGcac                                                                      <  2:1280855/98‑1 (MQ=255)
                                                                                                                       tACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGcac                                                                      >  1:1280855/1‑98 (MQ=255)
                                                                                                                       tACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGAt                  >  1:1169835/1‑150 (MQ=255)
                                                                                                                       tACGTCAGTGACTACGGTCAAAAACGCAGGCTGTTGGTTCGGTA‑GGGGTAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGAt                  <  2:1105182/150‑1 (MQ=255)
                                                                                                                            aacagCCTGCGGTTATGACCGTAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACt             >  1:58590/5‑150 (MQ=255)
                                                                                                                               tGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGt                                                                                >  2:148984/1‑80 (MQ=255)
                                                                                                                               tGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGt                                                                                <  1:148984/80‑1 (MQ=255)
                                                                                                                               agCCTGCGGTTATGACCGTAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCa          <  1:1238724/149‑1 (MQ=255)
                                                                                                                                 ccTGCGGTTATGACCGTAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGt        >  1:555032/1‑150 (MQ=255)
                                                                                                                                 ccTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGt        >  1:570055/1‑150 (MQ=255)
                                                                                                                                 ccTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGt        >  1:736163/1‑150 (MQ=255)
                                                                                                                                       gTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGTTCCTGc  >  2:1199836/1‑150 (MQ=255)
                                                                                                                                        tCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTcgc                                       <  2:639777/112‑1 (MQ=255)
                                                                                                                                        tCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTcgc                                       >  1:639777/1‑112 (MQ=255)
                                                                                                                                         |                                                                                                                                                    
TTGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGTTCCTGC  >  NC_002947/1818394‑1818678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑