Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,818,539	C→T	9.8%	R213R (CGC→CGT)	PP_1622 →	M23B subfamily metallopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,818,539	0	C	T	9.8%	155.1 / 6.3	61	R213R (CGC→CGT)	PP_1622	M23B subfamily metallopeptidase
Reads supporting (aligned to +/- strand):  ref base C (29/26);  new base T (3/3);  total (32/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01

TTGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGTTCCTGCCACGTCGTTG  >  NC_002947/1818394‑1818688                                                                                                                                                  |                                                                                                                                                       ttGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTGCGGTTATGACCGTAGGc                                                                                                                                                    >  1:1166828/1‑150 (MQ=255) ttGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGc                                                                                                                                                    >  1:1105182/1‑150 (MQ=255)  tGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTGCGGTTATGACCGTAGGca                                                                                                                                                   <  1:927585/150‑2 (MQ=255)  tGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTGCGGTTATGACCGTAGGca                                                                                                                                                   <  2:1166828/150‑2 (MQ=255)     tatCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGtt                                                                                                                                                <  2:86092/150‑1 (MQ=255)       tCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTgg                                                                                                                                              >  2:816425/1‑150 (MQ=255)          ccGGTGATTTGGGACAGCCTGTTTTCGCTGCGTATGATGGTGCGGTGGTTTATGCCGGGAGTGGGTTGAGGGGCTACGGCGAGCTGATCATCATCAAGAACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTc                                                                                                                                           >  1:943465/1‑150 (MQ=255)           cGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCg                                                                                                                                          <  2:1185361/150‑1 (MQ=255)              tGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTgggtttgta                                                                                                                                       <  2:403750/150‑8 (MQ=255)              tGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGa                                                                                                                                       <  2:1119007/150‑1 (MQ=255)              tGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGa                                                                                                                                       <  1:254076/150‑1 (MQ=255)               gATTTGGGTCAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATATCCGCAGGCTGTTGGTTCGGGA‑g                                                                                                                                     <  2:124/150‑1 (MQ=255)                 tttGGGACAGCCTGTTTTCGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑ggg                                                                                                                                   <  1:888282/150‑1 (MQ=255)                       aCAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGgcagca                                                                                                                             >  1:572989/1‑150 (MQ=255)                            cTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑gg                                                                                                                                    >  2:1213999/1‑138 (MQ=255)                            cTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑gg                                                                                                                                    <  1:1213999/138‑1 (MQ=255)                                      tgcGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAggcagggca                                                                                                              >  1:1004235/1‑150 (MQ=255)                                      tgcGTCTGATGGTGCGGCGGTTTATGCCGGGAGTGGCTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAggcagggca                                                                                                              >  2:197780/1‑150 (MQ=255)                                          tCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCg                                                                                                          >  1:559673/1‑150 (MQ=255)                                                  tGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTg                                                                                                                                               <  2:478937/106‑1 (MQ=255)                                                  tGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTg                                                                                                                                               >  1:478937/1‑106 (MQ=255)                                                    cGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCCTCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAgg                                                                                                                           >  2:5316/1‑123 (MQ=255)                                                    cGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAgg                                                                                                                           <  1:5316/123‑1 (MQ=255)                                                     ggtggtTTATGCCGGGAGTGGTTTGAGGGGCTCCGGAGAGCGGATCATAATCAAGCACAGGGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAAt                                                                                               <  2:943465/150‑1 (MQ=255)                                                         gtTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATgggg                                                                                           <  1:735900/150‑1 (MQ=255)                                                                 cGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACggg                                                                                   <  2:552501/150‑1 (MQ=255)                                                                      gTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACg                                                                                     >  2:890412/1‑143 (MQ=255)                                                                      gTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACg                                                                                     <  1:890412/143‑1 (MQ=255)                                                                          tttGAGGGGCTACGGCGAGCTGATCATCATCACGCACCGCAACACCTTCGGCAGTGCCTGCGGTCATAACTGCAGCACGGTCG‑CCGACATTTCGTTGCTGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACGAATCg                                                                          >  1:292489/1‑150 (MQ=255)                                                                             gAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGt                                                                       <  2:1004235/150‑1 (MQ=255)                                                                                gggCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGaa                                                                    <  1:816425/150‑1 (MQ=255)                                                                                                   atcatcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTcgc                                                 >  1:701807/1‑150 (MQ=255)                                                                                                      atcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTcgccgc                                              >  1:1021367/1‑150 (MQ=255)                                                                                                      atcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTcgccgc                                              <  2:559673/150‑1 (MQ=255)                                                                                                      atcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTc                                                   <  2:233087/145‑1 (MQ=255)                                                                                                      atcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTc                                                   >  1:233087/1‑145 (MQ=255)                                                                                                       tcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCgccgcc                                             >  1:66751/1‑150 (MQ=255)                                                                                                               aGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGATCGTTGGTTG‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACTGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCaaa                                     >  1:234705/1‑150 (MQ=255)                                                                                                                   acacCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCg                                 <  2:292489/150‑1 (MQ=255)                                                                                                                    cacCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGt                                <  2:66751/150‑1 (MQ=255)                                                                                                                       cTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGa                             <  2:572989/150‑1 (MQ=255)                                                                                                                       cTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAAGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTTGAGATTCGCCGCTAGGGCAAACCGGTcca                             >  2:7102/1‑148 (MQ=255)                                                                                                                        tACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGcac                                                                                <  2:1280855/98‑1 (MQ=255)                                                                                                                        tACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGcac                                                                                >  1:1280855/1‑98 (MQ=255)                                                                                                                        tACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGAt                            >  1:1169835/1‑150 (MQ=255)                                                                                                                        tACGTCAGTGACTACGGTCAAAAACGCAGGCTGTTGGTTCGGTA‑GGGGTAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGAt                            <  2:1105182/150‑1 (MQ=255)                                                                                                                             aacagCCTGCGGTTATGACCGTAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACt                       >  1:58590/5‑150 (MQ=255)                                                                                                                                tGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGt                                                                                          >  2:148984/1‑80 (MQ=255)                                                                                                                                tGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGt                                                                                          <  1:148984/80‑1 (MQ=255)                                                                                                                                agCCTGCGGTTATGACCGTAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCa                    <  1:1238724/149‑1 (MQ=255)                                                                                                                                  ccTGCGGTTATGACCGTAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGt                  >  1:555032/1‑150 (MQ=255)                                                                                                                                  ccTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGt                  >  1:736163/1‑150 (MQ=255)                                                                                                                                  ccTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGt                  >  1:570055/1‑150 (MQ=255)                                                                                                                                        gTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGTTCCTGc            >  2:1199836/1‑150 (MQ=255)                                                                                                                                         tCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTcgc                                                 >  1:639777/1‑112 (MQ=255)                                                                                                                                         tCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTcgc                                                 <  2:639777/112‑1 (MQ=255)                                                                                                                                           aTAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTCCGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGTTCCTGCCAc         >  1:1049531/1‑150 (MQ=255)                                                                                                                                           aTAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGTTCCTGCCAc         >  1:1128176/1‑150 (MQ=255)                                                                                                                                            tAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCCGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCACACCCGTCGATCCACTCCAGTTCCTGCCAcg        >  1:1173600/1‑150 (MQ=255)                                                                                                                                               ccGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGTTCCTGCCAcgtcg     <  2:570055/150‑1 (MQ=255)                                                                                                                                                cGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGTTCCTGCCAcgtcgt    >  1:505056/1‑150 (MQ=255)                                                                                                                                                  cAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGTTCCTGCCACGTCGTTg  >  1:930570/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                       TTGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGA‑GGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGTTCCTGCCACGTCGTTG  >  NC_002947/1818394‑1818688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑