Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,852,271 T→C 23.7% Y439H (TAC→CAC)  relA → ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,852,2710TC23.7% 91.7 / 27.1 55Y439H (TAC→CAC) relAATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base T (22/20);  new base C (7/6);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

AGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTGAACTC  >  NC_002947/1852122‑1852419
                                                                                                                                                     |                                                                                                                                                    
aGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTCCTTGGCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCt                                                                                                                                                      <  1:978962/150‑1 (MQ=255)
   tGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTAcc                                                                                                                                                   <  1:659225/150‑1 (MQ=255)
   tGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTAcc                                                                                                                                                   >  1:1286468/1‑150 (MQ=255)
       ggAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGt                                                                                                                                               <  1:969212/150‑1 (MQ=255)
             gCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAATGCGCCACGCCATTGGACTTCGTCTACCGCGTGCacac                                                                                                                                         >  2:527681/1‑150 (MQ=255)
             gCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCCACCGCGTGCacac                                                                                                                                         >  2:349838/1‑150 (MQ=255)
               aCGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCg                                                                                                                                       >  1:257224/1‑150 (MQ=255)
               aCGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCg                                                                                                                                       >  1:177929/1‑150 (MQ=255)
               aCGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCCACCGCGTGCACACCg                                                                                                                                       >  1:413461/1‑150 (MQ=255)
                       cTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGc                                                                                                                               <  2:132315/150‑1 (MQ=255)
                       cTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCCACCGCGTGCACACCGAGATCGGc                                                                                                                               <  1:131784/150‑1 (MQ=255)
                         gggCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCGCAGGCTCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCca                                                                                                                             <  1:732741/150‑1 (MQ=255)
                              aCATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACaatt                                                                                                                        <  2:1216293/150‑3 (MQ=255)
                                         ctggccGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGcgcg                                                                                                             >  1:62680/1‑150 (MQ=255)
                                              cGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGAt                                                                                                        >  1:737135/1‑150 (MQ=255)
                                                 gCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGAGCGAAGATCaa                                                                                                     >  1:380131/1‑150 (MQ=255)
                                                      tGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGcc                                                                                                <  1:932991/150‑1 (MQ=255)
                                                           gTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCg                                                                                                                                 <  2:117511/112‑1 (MQ=255)
                                                           gTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCg                                                                                                                                 >  1:117511/1‑112 (MQ=255)
                                                                cATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGcc                                                                                      >  1:524742/1‑150 (MQ=255)
                                                                 aTCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCCACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCg                                                                                     >  2:650507/1‑150 (MQ=255)
                                                                       ccTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCCACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAAc                                                                               <  1:575957/150‑1 (MQ=255)
                                                                             cGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCCACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGc                                                                         <  1:1193519/150‑1 (MQ=255)
                                                                                  ttATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCa                                                                    <  2:62680/150‑1 (MQ=255)
                                                                                     tgtgTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGAc                                                                 <  2:257224/150‑1 (MQ=255)
                                                                                       tgtTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTg                                                               >  2:239214/1‑150 (MQ=255)
                                                                                         ttCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGc                                                             <  2:380131/150‑1 (MQ=255)
                                                                                         ttCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCCACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGc                                                             <  1:650507/150‑1 (MQ=255)
                                                                                             cccccGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGCAGAGCAACGGCCGTATCGTCCCGCACAACTACAACCTCCACACTTGCCAGc                                                         >  2:437930/1‑150 (MQ=255)
                                                                                              ccccGGCGGCCACGCCATCGACCTGCCCAAAGGCGCCCCGCCATTGGACTTCGCCCACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCCCGACCAGCAACGGCCGTCTCGTGCCCCCGCACCACAGCCTGCACCCTGTCGAGcg                                                        >  2:381158/1‑149 (MQ=255)
                                                                                              ccccGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCa                                                        >  2:221347/1‑150 (MQ=255)
                                                                                                ccGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCa                                                        <  1:13815/148‑1 (MQ=255)
                                                                                                ccGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCa                                                        >  2:13815/1‑148 (MQ=255)
                                                                                                ccGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCCACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAgg                                                      >  2:580375/1‑150 (MQ=255)
                                                                                                 cGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGt                                                     <  1:239214/150‑1 (MQ=255)
                                                                                                       ccACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATc                                                                                           >  2:1146866/1‑106 (MQ=255)
                                                                                                       ccACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATc                                                                                           <  1:1146866/106‑1 (MQ=255)
                                                                                                           gCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCCACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGatcatc                                           >  2:507769/1‑150 (MQ=255)
                                                                                                             cATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCAc                                         >  2:415159/1‑150 (MQ=255)
                                                                                                                  aCCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCagca                                    >  2:1155053/1‑150 (MQ=255)
                                                                                                                         cAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATAGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGATATCATCACCAGCAAGCACgg                             <  1:712715/150‑1 (MQ=255)
                                                                                                                          aaaGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCGGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGc                            >  1:80467/1‑150 (MQ=255)
                                                                                                                          aaaGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTg                                                                                        >  2:876533/1‑90 (MQ=255)
                                                                                                                          aaaGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTg                                                                                        <  1:876533/90‑1 (MQ=255)
                                                                                                                             ggCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAAc                         <  2:737135/150‑1 (MQ=255)
                                                                                                                                gccacgccaTTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAAcccc                      >  2:1062472/1‑150 (MQ=255)
                                                                                                                                        aTTGGACTTCGCCCACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGa              >  1:960445/1‑150 (MQ=255)
                                                                                                                                           ggACTTCGCCCACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTg           <  1:507769/150‑1 (MQ=255)
                                                                                                                                              cTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGtt        <  1:527681/150‑1 (MQ=255)
                                                                                                                                               ttCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAg                                                          <  2:1273196/99‑1 (MQ=255)
                                                                                                                                               ttCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAg                                                          >  1:1273196/1‑99 (MQ=255)
                                                                                                                                               ttCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTg       >  1:662382/1‑150 (MQ=255)
                                                                                                                                                 cGCCCACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAACAGCTGATGACGTTCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTGaa     <  1:349838/150‑1 (MQ=255)
                                                                                                                                                  gtcTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGc                   <  2:349898/133‑1 (MQ=255)
                                                                                                                                                  gtcTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGc                   >  1:349898/3‑135 (MQ=255)
                                                                                                                                                    cTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTGAACTc  <  2:80467/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTGAACTC  >  NC_002947/1852122‑1852419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑