Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,888,711 C→T 13.6% S535N (AGC→AAC)  PP_1695 ← sodium‑solute symporter/two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,888,7110CT13.6% 104.0 / 8.9 44S535N (AGC→AAC) PP_1695sodium‑solute symporter/two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base C (18/20);  new base T (3/3);  total (21/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

TTCTGGTTAGGGTTGAAACCCTTGCCCTGGCGGTAGGCAAAGCGGATGAAGCTCTGCCGTGCGCGCTCTTCCCCGACAAAGCGCGAGGCCAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTGCGCGACAGCACCGAAACCCAGGCGAACAGGGTGAAGTTGCCCGCCAGTGACAGCACCACACCTTGGGTCAGTGGACTGATCGG  >  NC_002947/1888576‑1888859
                                                                                                                                       |                                                                                                                                                    
ttCTGGTTAGGGTTGAAACCCTTGCCCTGGCGGTAGGCAAAGCGGATGAAGCTCTGCCGTGCGCGCTCTTCCCCGACAAAGCGCGAGGCCAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTg                                                                                                                                        <  1:871351/150‑1 (MQ=255)
   tGGTTAGGGTCGAAACCCTTGCCCTGGCGGTAGGCAAATCGAATGAAGCTCTGCCGTGCGCGCTCTTCCCCGACAAAGCGCGAGGCCAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTc                                                                                                                                     <  1:804447/150‑1 (MQ=255)
    ggTTAGGGTTGAAACCCTTGCCCTGGCGGTAGGCAAAGCGGATGAAGCTCTGCCGTGCGCGCTCTTCCCCGACAAAGCGCGAGGCCAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTct                                                                                                                                    <  2:657224/150‑1 (MQ=255)
     gTTAGGGTTGAAACCCTTGCCCTGGCGGTAGGCAAAGCGGATGAAGCTCTGCCGTGCGCGCTCTTCCCCGACAAAGCGCGAGGCCAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTctg                                                                                                                                   >  2:1256541/1‑150 (MQ=255)
            ttGAAACCCTTGCCCTGGCGGTAGGCAAAGCGGATGAAGCTCTGCCGTGCGCGCTCTTCCCCGACAAAGCGCGAGGCCAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGcc                                                                                                                             >  2:832547/1‑149 (MQ=255)
            ttGAAACCCTTGCCCTGGCGGTAGGCAAAGCGGATGAAGCTCTGCCGTGCGCGCTCTTCCCCGACAAAGCGCGAGGCCAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGcc                                                                                                                             <  1:832547/149‑1 (MQ=255)
                aaCCCTTGCCCTGGCGGTAGGCAAAGCGGATGAAGCTCTGCCGTGCGCGCTCTTCCCCGACAAAGCGCGAGGCCAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGa                                                                                                                        >  2:418753/1‑150 (MQ=255)
                 aCCCTTGCCCTGGCGGTAGGCAAAGCGGATGAAGCTCTGCCGTGCGCGCTCTTCCCCGACAAAGCGCGAGGCCAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGaa                                                                                                                       >  2:864545/1‑150 (MQ=255)
                  cccTTGCCCTGGCGGTAGGCAAAGCGGATGAAGCTCTGCCGTGCGCGCTCTTCCCCGACAAAGCGCGAGGCCAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAAc                                                                                                                      <  2:258822/150‑1 (MQ=255)
                                      aaaGCGGATGAAGCTCTGCCGTGCGCGCTCTTCCCCGACAAAGCGCGAGGCCAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTc                                                                                                  <  1:864545/150‑1 (MQ=255)
                                      aaaGCGGATGAAGCTCTGCCGTGCGCGCTCTTCCCCGACAAAGCGCGAGGCCAGGGTCAGCAGGTCGTCGATCTGCAAGGCTAGTAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTc                                                                                                  >  1:22524/1‑150 (MQ=255)
                                           ggATGAAGCTCTGCCGTGCGCGCTCTTCCCCGACAAAGCGCGAGGCCAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGaaa                                                                                             >  2:1085537/1‑150 (MQ=255)
                                                aaGCTCTGCCGTGCGCGCTCTTCCCCGACAAAGCGCGAGGCCAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCcgc                                                                                        <  1:418753/150‑1 (MQ=255)
                                                  gCTCTGCCGTGCGCGCTCTTCCCCGACAAAGCGCGAGGCCAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGt                                                                                      <  2:1114183/150‑1 (MQ=255)
                                                    tctGCCGTGCGCGCTCTTCCCCGACAAAGCGCGAGGCCAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTgc                                                                                    >  1:150328/1‑150 (MQ=255)
                                                              gcgcTCTTCCCCGACAAAGCGCGAGGCCAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACcggccggc                                                                                                              <  2:839428/114‑1 (MQ=255)
                                                              gcgcTCTTCCCCGACAAAGCGCGAGGCCAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACcggccggc                                                                                                              >  1:839428/1‑114 (MQ=255)
                                                              gcgcTCTTCCCCGACAAAGCGCGAGGCCAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTGCGCGACAGCAc                                                                          >  1:794844/1‑150 (MQ=255)
                                                                        ccGACAAAGCGCGAGGCCAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTTCTGCTGGGCCGCGCGCTg                                                                                                                                        >  2:572226/1‑78 (MQ=255)
                                                                        ccGACAAAGCGCGAGGCCAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTTCTGCTGGGCCGCGCGCTg                                                                                                                                        <  1:572226/78‑1 (MQ=255)
                                                                        ccGACAAAGCGCGAGGCCAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGTTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTGCGCGACAGCACCGAAACCCAg                                                                >  1:416595/1‑150 (MQ=255)
                                                                        ccGACAAAGCGCGAGGCCAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGTTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTGCGCGACAGCACCGAAACCCAg                                                                >  1:416656/1‑150 (MQ=255)
                                                                        ccGACAAAGCGCGAGGCCAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGTTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTGCGCGACAGCACCGAAACCCAg                                                                >  1:417040/1‑150 (MQ=255)
                                                                         cGACAAAGCGCGAGGCCAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTGCGCGACa                                                                              >  1:142460/1‑135 (MQ=255)
                                                                         cGACAAAGCGCGAGGCCAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTGCGCGACa                                                                              <  2:142460/135‑1 (MQ=255)
                                                                          gACAAAGCGCGAGGCCAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTGCGCGACAGCACCGAAACCCAGGc                                                              <  1:442952/150‑1 (MQ=255)
                                                                                  gcgAGGCCAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTGCGCGACAGCACCGAAACCCAGGCGAACAggg                                                      >  1:1075080/1‑150 (MQ=255)
                                                                                         cAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTGCGCGACAGCACCGAAACCCAg                                                                <  1:527677/133‑1 (MQ=255)
                                                                                         cAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTGCGCGACAGCACCGAAACCCAg                                                                >  2:527677/1‑133 (MQ=255)
                                                                                          aGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTGCGCGACAGCACCGAAACCCAGGCGAACAGGGTGAAGTTg                                              >  1:266564/1‑150 (MQ=255)
                                                                                            ggTCAGCAGGTCGTCGATCTGCACCGCCCGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTGCGCGACAGCACCGAAACCCAGGCGAACAGGGTGAAGTTGcc                                            <  2:163028/150‑1 (MQ=255)
                                                                                                 gcagGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGTTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTGCGCGACAGCACCGAAACCCAGGCGAACAGGGTGAAGTTGCCCGCCa                                       <  2:416595/150‑1 (MQ=255)
                                                                                                 gcagGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGTTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTGCGCGACAGCACCGAAACCCAGGCGAACAGGGTGAAGTTGCCCGCCa                                       <  2:416656/150‑1 (MQ=255)
                                                                                                 gcagGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGTTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTGCGCGACAGCACCGAAACCCAGGCGAACAGGGTGAAGTTGCCCGCCa                                       <  2:417040/150‑1 (MQ=255)
                                                                                                 gcagGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTGCGCGACAGCACCGAAACCCAGGCGAACAGGGTGAAGTTGCCCGCCa                                       <  2:1075080/150‑1 (MQ=255)
                                                                                                     gtcgtcGATCTGCACCGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTGCGCGACAGCACCGAAACCCAGGCGAACAGGGTGAAGTTGCCCGCCAGTGa                                   >  2:800439/1‑150 (MQ=255)
                                                                                                         tcgATCTGCACCGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTGCGCGACAGCACCGAAACCCAGGCGAACAGGGTGAAGTTGCCCGCCAGTGACAGc                               <  1:1085537/150‑1 (MQ=255)
                                                                                                                    cGCCCGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGGCTCCCAGTGCTGGGAAAGGCGCATGCGCGGCAGCACCGAAACCCAGGCGAACAGGGTGAAGTTGCCCGCCAGTGACAGCACCACACCTTg                    >  1:47776/1‑150 (MQ=255)
                                                                                                                    cGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTGCGCGACAGCACCGAAACCCAGGCGAACAGGGTGAAGTTGCCCGCCAGTGACAGCACCACACCTTg                    <  2:794844/150‑1 (MQ=255)
                                                                                                                      ccAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTGCGCGACAGCACCGAAACCCAGGCGAACa                                                         <  2:36433/111‑1 (MQ=255)
                                                                                                                      ccAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTGCGCGACAGCACCGAAACCCAGGCGAACa                                                         >  1:36433/1‑111 (MQ=255)
                                                                                                                            gcGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTGCGCGACAGCACCGAAACCCAGGCGAACAGGGTGAAGTTGCCCGCCAGTGACAGCACCACACCTTGGGTCAGTg            <  1:1024127/150‑1 (MQ=255)
                                                                                                                               gCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTGCGCGACAGCACCGAAACCCAGGCGAACAGGGTGAAGTTg                                              <  1:593930/113‑1 (MQ=255)
                                                                                                                               gCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTGCGCGACAGCACCGAAACCCAGGCGAACAGGGTGAAGTTg                                              >  2:593930/1‑113 (MQ=255)
                                                                                                                                     tgctgGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTGCGCGACAGCACCGAAACCCAGGCGAACAGGGTGAAGTTGCCCGCCAGTGACAGcac                             >  2:484242/1‑124 (MQ=255)
                                                                                                                                     tgctgGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTGCGCGACAGCACCGAAACCCAGGCGAACAGGGTGAAGTTGCCCGCCAGTGACAGcac                             <  1:484242/124‑1 (MQ=255)
                                                                                                                                      gctgGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTGCGCGACAGCACCGAAACCCAGGCGAACAGGGTGAAGTTGCCCGCCAGTGACAGCACCACACCTTGGGTCAGTGGACTGATCgg  >  1:411749/1‑150 (MQ=255)
                                                                                                                                       |                                                                                                                                                    
TTCTGGTTAGGGTTGAAACCCTTGCCCTGGCGGTAGGCAAAGCGGATGAAGCTCTGCCGTGCGCGCTCTTCCCCGACAAAGCGCGAGGCCAGGGTCAGCAGGTCGTCGATCTGCACCGCCAGCAGCGGCTTGCTGCTGGGCCGCGCGCTGGTCTGCTGGCCAATGAACCGGCCGGCCTGCCAGTGCTCGGAAACCCGCGTGCGCGACAGCACCGAAACCCAGGCGAACAGGGTGAAGTTGCCCGCCAGTGACAGCACCACACCTTGGGTCAGTGGACTGATCGG  >  NC_002947/1888576‑1888859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑