| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,952,761 | G→T | 6.9% | L275M (CTG→ATG) | mnmC ← | methyltransferase/FAD‑dependent oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,952,761 | 0 | G | T | 6.9% | 183.8 / 2.3 | 58 | L275M (CTG→ATG) | mnmC | methyltransferase/FAD‑dependent oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (29/25); new base T (2/2); total (31/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01 | |||||||||||
CCGAACCCGGACAGGATCATCTGCGACAGCGCGGTGCCATGGGCAGACAGCTTGAGGTACAGCACCCCTTGTGGGTTGCCCGAAGCCTCCTGGGCGGGGGCTTCGTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGGCGCGCATGGGCCGGGCAGGGGGCCGACGTAGGCGCCGCTCATCACCTCCCACTTCTTGC > NC_002947/1952616‑1952910 | ccGAACCCGGACAGGATCATCTGCGACAGCGCGGTGCCATGGGCAGACAGCTTGAGGTACAGCACCCCTTGTGGGTTGCCCGAAGCCTCCTGGGCGGGGGCTTCGTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTg > 1:127624/1‑150 (MQ=255)ccGAACCCGGACAGGATCATCTGCGACAGCGCGGTGCCATGGGCAGACAGCTTGAGGTACAGCACCCCTTGTGGGTTGCCCGAAGCCTCCTGGGCGGGGGCTTCGTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTg > 1:37284/1‑150 (MQ=255) cccGGACAGGATCATCTGCGAGAGCGCGGTGCCATGGGCAGACAGCTGGAGGTACAGCACCCCTTGTGGGTTGCCCGAAGCCTCCTGGGCGGGGGCTTCGTGGCGCTCCAGCACGGTTACCTGCCAGCCGCTCCGGGCCAGGCTggcggc > 2:174547/1‑150 (MQ=255) cccGGACAGGATCATCTGCGACAGCGCGGTGCCATGGGCAGACAGCTTGAGGTACAGCACCCCTTGTGGGTTGCCCGAAGCCTCCTGGGCGGGGGCTTCGTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTggcggc < 1:817442/150‑1 (MQ=255) tcTGCGACAGCGCGGTGCCATGGGCAGACAGCTTGAGGTACAGCACCCCTTGTGGGTTGCCCGAAGCCTCCTGGGCGGGGGCTTCGTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCgg > 1:752035/1‑150 (MQ=255) tcTGCGACAGCGCGGTGCCATGGGCAGACAGCTTGAGGTACAGCACCCCTTGTGGGTTGCCCGAAGCCTCCTGGGCGGGGGCTTCGTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCg < 2:509194/149‑1 (MQ=255) tcTGCGACAGCGCGGTGCCATGGGCAGACAGCTTGAGGTACAGCACCCCTTGTGGGTTGCCCGAAGCCTCCTGGGCGGGGGCTTCGTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCg > 1:509194/1‑149 (MQ=255) gCGACAGCGCGGTGCCATGGGCAGACAGCTTGAGGTACAGCACCCCTTGTGGGTTGCCCGAAGCCTCCTGGGCGGGGGCTTCGTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCaa < 2:1225920/150‑1 (MQ=255) gCGACAGCGCGGTGCCATGGGCAGACAGCTTGAGGTACAGCACCCCTTGTGGGTTGCCCGAAGCCTCCTGGGCGGGGGCTTCGTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCaa > 1:489100/1‑150 (MQ=255) ggTGCCATGGGCAGACAGCTTGAGGTACAGCACCCCTTGTGGGTTGCCCGAAGCCTCCTGGGCGGGGGCTTCGTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCAcc > 2:968716/1‑150 (MQ=255) cATGGGCAGACAGCTTGAGGTACAGCACCCCTTGTGGGTTGCCCGAAGCCTCCTGGGCGGGGGCTTCGTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCAc < 1:1017281/144‑1 (MQ=255) cATGGGCAGACAGCTTGAGGTACAGCACCCCTTGTGGGTTGCCCGAAGCCTCCTGGGCGGGGGCTTCGTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCAc > 2:1017281/1‑144 (MQ=255) aTGGGCAGACAGCTTGAGGTACAGCACCCCTTGTGGGTTGCCCGAAGCCTCCTGGGCGGGGGCTTCGTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTcc < 1:457872/138‑1 (MQ=255) aTGGGCAGACAGCTTGAGGTACAGCACCCCTTGTGGGTTGCCCGAAGCCTCCTGGGCGGGGGCTTCGTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTcc > 2:457872/1‑138 (MQ=255) gACAGCTTGAGGTACAGCACCCCTTGTGGGTTGCCCGAAGCCTCCTGGGCGGGGGCTTCGTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGcc > 1:247627/1‑150 (MQ=255) gggTACAGCACCCCTTGTGGGTTGCCCGAAGCCTCCTGGGCGGGGGCTTCGTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGc < 2:489100/149‑1 (MQ=255) gtgGGTTGCCCGAAGCCTCCTGGGCGGGGGCTTCGTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCGAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCAACGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCgg > 1:843504/1‑150 (MQ=255) tgGGTTGCCCGAAGCCTCCTGGGCGGGGGCTTCGTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCggg < 1:174547/150‑1 (MQ=255) gTTGCCCGAAGCCTCCTGGGCGGGGGCTTCGTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCATGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGgcg > 1:987467/1‑150 (MQ=255) gTTGCCCGAAGCCTCCTGGGCGGGGGCTTCGTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCATGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGgcg > 1:987241/1‑150 (MQ=255) gAAGCCTCCTGGGCGGGGGCTTCGTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTAc > 1:588154/1‑150 (MQ=255) cctcctGGGCGGGGGCTTCGTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAg < 2:247627/150‑1 (MQ=255) tcctGGGCGGGGGCTTCGTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGgc > 1:333357/1‑150 (MQ=255) gCGGGGGCTTCGTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCATGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGGCGCGCAt < 2:987467/150‑1 (MQ=255) gCGGGGGCTTCGTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCATGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGGCGCGCAt < 2:987241/150‑1 (MQ=255) cGGGGGCTTCGTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGGCGCGCATg < 2:101033/150‑1 (MQ=255) ggaggCTTCGTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGGCGCGCATgg < 2:1284871/147‑1 (MQ=255) gTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCa > 1:1195530/1‑86 (MQ=255) gTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCa < 2:1195530/86‑1 (MQ=255) gTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGGCGCGCATGGGCCGGGCAg < 2:543653/150‑1 (MQ=255) gTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGGCGCGCATGGGCCGGGCAg > 2:611076/1‑150 (MQ=255) ggCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGGCGCGCATGGGCCGGGCAggg < 2:127624/150‑1 (MQ=255) ggCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGGCGCGCATGGGCCGGGCAggg < 2:1039409/150‑1 (MQ=255) gcgcTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGGCGCGCATGGGCCGGGCAgggg < 1:968716/150‑1 (MQ=255) gcTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCgg < 1:832994/94‑1 (MQ=255) gcTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCgg > 2:832994/1‑94 (MQ=255) gcTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGGCGCGCATGGGCCGGGCAGGGGGc > 2:558430/1‑150 (MQ=255) tCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCCGGCTGGGGGCGATGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAAGCGCGCATGGGCCGGGCAGGGGGCCg > 1:543033/1‑150 (MQ=255) aGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGgcgc < 2:652564/125‑1 (MQ=255) aGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGgcgc > 1:652564/1‑125 (MQ=255) gCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGGCGCGCATGGGCCGGGCAGGGGGCCGACGt > 2:144179/1‑150 (MQ=255) ttACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGGCGCGCATGGGCCGGGCAGGGGGCCGACGTAGgcgc > 2:701672/1‑150 (MQ=255) ttACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGGCGCGCATGGGCCGGGCAGGGGGCCGACGTAGgcgc < 1:999490/150‑1 (MQ=255) aCCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGAt < 1:304181/62‑1 (MQ=255) aCCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGAt > 2:304181/1‑62 (MQ=255) cTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGGCGCGCATGGGCCGGGCAGGGGGc > 2:321897/1‑134 (MQ=255) cTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGGCGCGCATGGGCCGGGCAGGGGGc < 1:321897/134‑1 (MQ=255) cTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGGCGCGCATGGGCCGGGCAGGGGGCCGGCGTa > 2:1155700/1‑141 (MQ=255) cTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGGCGCGCATGGGCCGGGCAGGGGGCCGGCGTa < 1:1155700/141‑1 (MQ=255) ccAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGGCGCGCATGGGCCGGGCAggggg > 1:739393/1‑130 (MQ=255) ccAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGGCGCGCATGGGCCGGGCAggggg < 2:739393/130‑1 (MQ=255) ccAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGGCGCGCATGGGCCGGGCAGGGGGCCGACGTAGGCGCCGCtcat > 2:1197684/1‑150 (MQ=255) cAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGGCGCGCATGGGCCGGGCAGGGGGCCGACGTAGGCGCCGCtcatc < 2:264249/150‑1 (MQ=255) aGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGGCGCGCATGGGCCGGGCAGGGGGCCGACGTAGGCGCCGCtcatca > 2:816205/1‑150 (MQ=255) ccGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGGCGCGCATGGGCCGGGCAGGGGGCCGACGTAGGCGCCGCTCATCAcc < 2:543033/150‑1 (MQ=255) gcgcCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGGCGCGCATGGGCCg < 2:818859/115‑1 (MQ=255) gcgcCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGGCGCGCATGGGCCg > 1:818859/1‑115 (MQ=255) ccAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGGCGCGCATGGGCCGGGCAGGGGGCCGACGTAGGCGCCGCTCATCACCTCCCActtct > 1:636375/1‑150 (MQ=255) ggCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGGCGCGCATGGGCCGGGCAGGGGGCCGACGTAGGCGCCGCTCATCACCTCCCACTTCTTGc > 2:122194/1‑150 (MQ=255) | CCGAACCCGGACAGGATCATCTGCGACAGCGCGGTGCCATGGGCAGACAGCTTGAGGTACAGCACCCCTTGTGGGTTGCCCGAAGCCTCCTGGGCGGGGGCTTCGTGGCGCTCCAGCACGGTTACCTGCCAGCCGCGCCGGGCCAGGCTGGCGGCGCTGCTGCTGCCGGCAAGTCCGGCACCGATCACCAGCGCCTCGCGCGGCCCCTGTGTCACCGCGGGGCGCGCGTACCAAGGCGCGCATGGGCCGGGCAGGGGGCCGACGTAGGCGCCGCTCATCACCTCCCACTTCTTGC > NC_002947/1952616‑1952910 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |