| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,968,712 | G→C | 9.4% | A50A (GCC→GCG) | gph ← | phosphoglycolate phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,968,712 | 0 | G | C | 9.4% | 157.6 / 8.5 | 55 | A50A (GCC→GCG) | gph | phosphoglycolate phosphatase |
| Reads supporting (aligned to +/- strand): ref base G (26/23); new base C (2/3); total (29/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01 | |||||||||||
CGATGTCGGCCAGCAGCTCGGCCATGCCCTCGAACAGCTTGCTGTGCACCGCGCAGTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCGTGTCCAGCAAGGTGCCGTCCATGTCGAAGAGTACTGCTC > NC_002947/1968564‑1968851 | cGATGTCGGCCAGCAGCTCGGCCATGCCCTCGAACAGCTTGCTGTGCACCGCGCAGTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCggg < 2:571241/150‑1 (MQ=255) aTGTCGGCCAGCAGCTCGGCCATGCCCTCGAACAGCTTGCTGTGCACCGCGCAGTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGgcg > 2:779485/1‑150 (MQ=255) tCGGCCAGCAGCTCGGCCATGCCCTCGAACAGCTTGCTGTGCACCGCGCAGTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCa < 1:668042/150‑1 (MQ=255) ggCCAGCAGCTCGGCCATGCCCTCGAACAGCTTGCTGTGCACCGCGCAGTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCagc > 1:1214646/1‑148 (MQ=255) ggCCAGCAGCTCGGCCATGCCCTCGAACAGCTTGCTGTGCACCGCGCAGTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCCCGCGCCatg > 1:106055/1‑148 (MQ=255) ccAGCAGCTCGGCCATGCCCTCGAACAGCTTGCTGTGCACCGCGCAGTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCCCGCGCCatggt < 2:880668/150‑5 (MQ=255) cagcagCTCGGCCATGCCCTCGAACAGCTTGCTGTGCACCGCGCAGTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCCCGCGCCatggtt < 2:106055/150‑6 (MQ=255) gcagcTCGGCCATGCCCTCGAACAGCTTGCTGTGCACCGCGCAGTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCgaga > 1:216149/1‑150 (MQ=255) agcTCGGCCATGCCCTCGAACAGCTTGCTGTGCACCGCGCAGTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCCCGGCGCGAGCACCCCCCGCGATc > 2:332498/1‑150 (MQ=255) gcTCGGCCATGCCCTCGAACAGCTTGCTGTGCACCGCGCAGTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCggg > 2:115136/1‑135 (MQ=255) gcTCGGCCATGCCCTCGAACAGCTTGCTGTGCACCGCGCAGTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCggg < 1:115136/135‑1 (MQ=255) tCGGCCATGCCCTCGAACAGCTTGCTGTGCACCGCGCAGTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCCCGCGCCatgg > 2:500307/1‑138 (MQ=255) tCGGCCATGCCCTCGAACAGCTTGCTGTGCACCGCGCAGTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCCCGCGCCatgg < 1:500307/141‑4 (MQ=255) ccTCGAACAGCTTGCTGTGCACCGCGCAGTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCa < 1:1070996/150‑1 (MQ=255) tCGAACAGCTTGCTGGGCACCGCGCAGTCGCGCTGGTAACGCTCGAGGAACTCGAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAgg < 1:452209/150‑1 (MQ=255) gAACAGCTTGCTGTGCACCGCGCAGTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGtt < 2:200849/150‑1 (MQ=255) aaCAGCTTGCTGTGCACCGCGCAGTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTg > 1:593948/1‑150 (MQ=255) aaCAGCTTGCTGTGCACCGCGCAGTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTAACCCTCCGCCGCTCGCGCGGAGGCGGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTg > 1:610787/1‑150 (MQ=255) cTGTGCACCGCGCAGTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAAcg < 2:216149/150‑1 (MQ=255) tgtgCACCGCGCAGTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGtt < 1:465067/139‑1 (MQ=255) tgtgCACCGCGCAGTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGtt > 2:465067/1‑139 (MQ=255) tgCACCGCGCAGTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAAcggcc < 1:580818/150‑1 (MQ=255) cgcgCAGTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAg > 1:917432/1‑150 (MQ=255) cgcgAAGTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAg > 2:720383/1‑150 (MQ=255) gcAGTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGTAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAAcggccgg > 1:1057205/1‑144 (MQ=255) gcAGTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGTAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAAcggccgg < 2:1057205/144‑1 (MQ=255) gTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAg > 2:340777/1‑150 (MQ=255) tCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGcc > 1:203889/1‑150 (MQ=255) gAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAAcg < 1:171137/118‑1 (MQ=255) gAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAAcg > 2:171137/1‑118 (MQ=255) aGGAACTCCAGGCGCAGGGGCTCGAAGCCGTAAGAATCGGGATCCGTGGCGGACGATAAGGGAACCATGGCGCGGGCGGCACCCGAGATCACGCCACGCATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTgg > 2:468934/1‑150 (MQ=255) aCTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCaaa > 2:675658/1‑150 (MQ=255) ccAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATgg < 2:1224625/150‑1 (MQ=255) cAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGc > 2:701296/1‑150 (MQ=255) aGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCg > 1:879619/1‑150 (MQ=255) gcgcAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCTCGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGAt < 1:477587/150‑1 (MQ=255) gCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCgt < 2:203889/150‑1 (MQ=255) cGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGa < 2:805281/111‑1 (MQ=255) cGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGa > 1:805281/1‑111 (MQ=255) gTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCGTGTc > 2:445624/1‑150 (MQ=255) cAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACCGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGGCGTGTCCa < 2:1214646/150‑1 (MQ=255) gCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCgtatttgct < 1:701296/150‑8 (MQ=255) cTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCGTGTCCAGCaa > 1:711712/1‑150 (MQ=255) tCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCGTGTCCAGCAAg > 1:914334/1‑150 (MQ=255) cGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCGTGTCCAGCAAGGt < 2:711712/150‑1 (MQ=255) tCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAGGTCCGGCGCCGTGTCCAGCAAGGTGCCg < 1:675658/150‑1 (MQ=255) cGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCGTGTCCAGCAAGGTGCCGTCCATGTCGaa < 1:445624/150‑1 (MQ=255) gggCAACCATGCCGCGGCCGCCACCCGAGATCACGCCACGGCTCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCGTGTCCAGCAAGGTGCCGTCCATGTCGAAGAGTa < 1:332498/149‑1 (MQ=255) cAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCGTGTCCAGCAAGGTGCCGTCCATGTCGAAGAGTACTg > 1:554509/1‑150 (MQ=255) aaCCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTc > 2:102231/1‑72 (MQ=255) aaCCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTc < 1:102231/72‑1 (MQ=255) aaCCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCGTGTCCAGCAAGGTGCCGTCCAt > 2:113157/1‑135 (MQ=255) aaCCATGGCGCGGGCGCCACCCGAGATCACGCCACGGAGCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCGTGTCCAGCAAGGTGCCGTCCAt < 1:113157/135‑1 (MQ=255) ccATGGCGCGGGCGCCACCTGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCGTGTCCAGCAAGGTGCCGTCCATGTCGAAGAGTACTGCTc > 2:799069/1‑150 (MQ=255) ccATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCGTGTCCAGCAAGGTGCCGTCCATGTCGaa > 1:217383/1‑139 (MQ=255) ccATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCGTGTCCAGCAAGGTGCCGTCCATGTCGaa < 2:217383/139‑1 (MQ=255) aTGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGAt > 1:1213667/1‑95 (MQ=255) aTGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGAt < 2:1213667/95‑1 (MQ=255) | CGATGTCGGCCAGCAGCTCGGCCATGCCCTCGAACAGCTTGCTGTGCACCGCGCAGTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCGTGTCCAGCAAGGTGCCGTCCATGTCGAAGAGTACTGCTC > NC_002947/1968564‑1968851 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |