| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,049,346 | G→T | 10.2% | intergenic (‑32/+28) | PP_1822 ← / ← folEA‑I | hypothetical protein/GTP cyclohydrolase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,049,346 | 0 | G | T | 10.2% | 152.1 / 5.2 | 49 | intergenic (‑32/+28) | PP_1822/folEA‑I | hypothetical protein/GTP cyclohydrolase I |
| Reads supporting (aligned to +/- strand): ref base G (23/21); new base T (3/2); total (26/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01 | |||||||||||
GCTCGATGCGGGCCTGGGCGGCGGCGTCGCGTTTGCGCTTGGCCGGGCGGCTGATCCAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCTTCTCGACACCGCGCATCATCATGCACATGT > NC_002947/2049203‑2049495 | gCTCGATGCGGGCCTGGGCGGCGGCGTCGCGTTTGCGCTTGGCCGGGCGGCTGATCCAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGt < 2:1144829/150‑1 (MQ=255) cTCGATGCGGGCCTGGGCGGCGGCGTCGCGTTTGCGCTTGGCCGGGCGGCTGATCCAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTc > 1:371070/1‑150 (MQ=255) tCGATGCGGGCCTGGGCGGCGGCGTCGCGTTTGCGCTTGGCCGGGCGGCTGATCCAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCg > 1:540692/1‑150 (MQ=255) gCGGGCCTGGGCGGCGGCGTCGCGTTTGCGCTTGGCGGGACCACTGATCGAGTCGCCGAAGACGATGAGCTCAGAGGGGCCAACCCGCAGGGCTTTGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTTCAGGGTCGGCttt < 1:287201/150‑3 (MQ=255) gCGGGCCTGGGCGGCGGCGTCGCGTTTGCGCTTGGCCGGGCGGCTGATCCAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCg < 1:39379/150‑1 (MQ=255) cgTCGCGTTTGCGCTTGGCCGGGCGGCTGATCCAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCa < 1:1148135/150‑1 (MQ=255) gcTTGGCCGGGCGGCTGATCCAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCt < 1:56001/150‑1 (MQ=255) cTGATCCAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGc < 2:1203631/150‑1 (MQ=255) aTCCAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCg > 2:1213762/1‑143 (MQ=255) aTCCAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCg < 1:1213762/143‑1 (MQ=255) ccAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTg > 1:936884/1‑150 (MQ=255) aGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTggc < 2:229065/150‑1 (MQ=255) cGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTAGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCTGGTGGCGGCGtt > 2:239119/1‑150 (MQ=255) cGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTcgcg < 1:319038/150‑1 (MQ=255) gATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCt < 2:570330/128‑1 (MQ=255) gATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCt > 1:570330/1‑128 (MQ=255) gCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTg < 1:279841/130‑1 (MQ=255) gCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTg > 2:279841/1‑130 (MQ=255) cGAGGCCAACCCGCAGGGCTTTGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTTCAGGGTCGGCttt > 2:1040665/1‑74 (MQ=38) cGAGGCCAACCCGCAGGGCTTTGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTTCAGGGTCGGCttt < 1:1040665/76‑3 (MQ=38) cGAGGCCAACCCGCAGGGCTTTGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCaa < 1:1166497/86‑1 (MQ=255) cGAGGCCAACCCGCAGGGCTTTGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCaa > 2:1166497/1‑86 (MQ=255) aGGCCAACCCGCAGGGCTTTGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCa < 1:271823/150‑1 (MQ=255) aaCCCGCAGGGCTTTGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTg < 1:1172332/90‑1 (MQ=255) aaCCCGCAGGGCTTTGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTg > 2:1172332/1‑90 (MQ=255) ccGCAGGGCTTTGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAg > 1:244095/1‑150 (MQ=255) gCAGGGCTTTGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGAGGGGCACGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGt > 1:977824/1‑150 (MQ=255) tGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCAcc < 1:1002865/126‑1 (MQ=255) tGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCAcc > 2:1002865/1‑126 (MQ=255) gACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTc > 1:389047/1‑150 (MQ=255) gATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGaa > 2:138328/1‑150 (MQ=255) gggCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGc > 2:1123928/1‑150 (MQ=255) aaCTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCTtct > 2:959041/1‑150 (MQ=255) tCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTc < 1:342888/140‑1 (MQ=255) tCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTc > 2:342888/1‑140 (MQ=255) tGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGa < 2:1201317/118‑1 (MQ=255) tGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGa > 1:1201317/1‑118 (MQ=255) tGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAg > 2:1187299/1‑119 (MQ=255) tGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAg < 1:1187299/119‑1 (MQ=255) tGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCTTCTCGACACCgcgc < 2:389047/150‑1 (MQ=255) ccgaGCCGACCCTTCAGGGTCGGCTTGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGAGTTGTCACGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCTTCTCGACACCGCGcatc > 1:22169/4‑150 (MQ=255) aaaaGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCTTCTCGACACCGCGcatc > 2:1245775/1‑150 (MQ=255) aaaaGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCTTCTCGACACCGCGcatc > 2:989925/1‑150 (MQ=255) gCCGACCCTGAAGGGTCGGCTCGTGGCCAGCAACCAATCACTTGATCAGCCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAACATCACCTAGGTGATCATTGTCGAATTCTGCTTCTCGACACCGCGcatcatca < 1:989925/150‑1 (MQ=255) gCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCTTCTCGACACCGCGcatcatca < 2:540692/150‑1 (MQ=255) ccGACCCTTCAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCTTCTCGACACCGCGcatcatcat > 1:111685/1‑150 (MQ=255) cGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGT‑GCCGCGGTGTCGCGGGAATCACCCCGCATCAACCAGGTGATCATGGTCGAATTCTGCTTCCCGACACCGCGCATCATCATgg > 1:200716/1‑149 (MQ=255) tGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCTTCTCGACACCGCGcatcat > 1:730365/1‑140 (MQ=255) tGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCTTCTCGACACCGCGcatcat < 2:730365/140‑1 (MQ=255) gAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCTTCTCGacac < 2:241663/129‑1 (MQ=255) gAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCTTCTCGacac > 1:241663/1‑129 (MQ=255) gAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCTTCTCGACACCGCGCATCATCATGCACAtgt > 1:1139984/1‑150 (MQ=255) | GCTCGATGCGGGCCTGGGCGGCGGCGTCGCGTTTGCGCTTGGCCGGGCGGCTGATCCAGTCACCGAAGACGATGAGCTGGCCGAGGCCAACCCGCAGGGCTTTGACGATCATGGGCAACTCCTGAAATGCAAAAGCCGACCCTGAAGGGTCGGCTCGGGGCCAGCAACCAATCACTTGATCAGGCTGAGGAACTCGCTGCGGGTGGCGGCGTTGTCGCGGAACTCACCCAGCATCACCGAGGTGATCATGGTCGAATTCTGCTTCTCGACACCGCGCATCATCATGCACATGT > NC_002947/2049203‑2049495 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |