Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,154,434 T→A 5.9% Q127H (CAA→CAT)  PP_1909 ← septum formation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,154,4340TA 5.9% 206.2 / 3.2 67Q127H (CAA→CAT) PP_1909septum formation protein
Reads supporting (aligned to +/- strand):  ref base T (34/29);  new base A (2/2);  total (36/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TCTGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGTCCGAGCCTA  >  NC_002947/2154292‑2154582
                                                                                                                                               |                                                                                                                                                    
tctGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCgg                                                                                                                                               <  2:864310/150‑1 (MQ=255)
tctGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCgg                                                                                                                                               >  2:500647/1‑150 (MQ=255)
 ctGGAACAAGCTCACCCCCAGCCCATCTGACCTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGt                                                                                                                                              >  1:997631/1‑150 (MQ=255)
      aCAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAGAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGgcgc                                                                                                                                         >  2:762321/1‑150 (MQ=255)
           cTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTc                                                                                                                                    >  2:902466/1‑150 (MQ=255)
            tCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTca                                                                                                                                   >  2:805288/1‑150 (MQ=255)
               cccccAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTcagca                                                                                                                                <  2:1124640/150‑1 (MQ=255)
                 cccAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCCGCGCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGt                                                                                                                              <  2:1067416/150‑1 (MQ=255)
                  ccAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTg                                                                                                                             >  1:968325/1‑150 (MQ=255)
                  ccAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTg                                                                                                                             >  1:256018/1‑150 (MQ=255)
                      ccccTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAg                                                                                                                         >  1:320799/1‑150 (MQ=255)
                               cTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAg                                                                                                                >  1:861213/1‑150 (MQ=255)
                               cTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAg                                                                                                                >  2:207412/1‑150 (MQ=255)
                                ttGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTcagca                                                                                                                                >  1:1139357/1‑133 (MQ=255)
                                ttGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTcagca                                                                                                                                <  2:1139357/133‑1 (MQ=255)
                                  gAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGaaa                                                                                                             <  2:1147489/150‑1 (MQ=255)
                                  gAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGaaa                                                                                                             <  1:401067/150‑1 (MQ=255)
                                     aCTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCg                                                                                                                                                >  1:1274148/1‑112 (MQ=255)
                                     aCTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCg                                                                                                                                                <  2:1274148/112‑1 (MQ=255)
                                     aCTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCt                                                                                                          >  1:649431/1‑150 (MQ=255)
                                        cccTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCAc                                                                                                       <  2:320799/150‑1 (MQ=255)
                                             cgcAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACctgct                                                                                                  <  1:207412/150‑1 (MQ=255)
                                               cAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCtgctgc                                                                                                <  2:968325/150‑1 (MQ=255)
                                                  tCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTcagca                                                                                                                                <  2:588279/115‑1 (MQ=255)
                                                  tCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTcagca                                                                                                                                >  1:588279/1‑115 (MQ=255)
                                                    cAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACctgctgtcctct                                                                                           <  2:649431/150‑7 (MQ=255)
                                                          cTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTc                                                                                     <  2:256018/150‑1 (MQ=255)
                                                          cTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTc                                                                                     <  1:500647/150‑1 (MQ=255)
                                                                        cGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCAGCTGCACTCAAGTAGTTgatagt                                                                       >  2:1035972/1‑145 (MQ=255)
                                                                              gCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTc                                                                                     <  2:1283588/130‑1 (MQ=255)
                                                                              gCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTc                                                                                     >  1:1283588/1‑130 (MQ=255)
                                                                                 cGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAGATCTCGCCA‑CGCCGCGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCt                                                              <  2:997631/150‑1 (MQ=255)
                                                                                   aCCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGcc                                                                                                                       >  2:308330/1‑91 (MQ=255)
                                                                                   aCCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGcc                                                                                                                       <  1:308330/91‑1 (MQ=255)
                                                                                         tccctgctcAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTa                                                      >  1:255762/1‑150 (MQ=255)
                                                                                         tccctgctcAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTa                                                      >  2:82985/1‑150 (MQ=255)
                                                                                           cctgctcAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATg                                                    >  2:471242/1‑150 (MQ=255)
                                                                                            ctgctcAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGc                                                   >  2:1052190/1‑150 (MQ=255)
                                                                                                tcAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCt                                               >  1:507844/1‑150 (MQ=255)
                                                                                                    ttCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAgc                                                                   >  1:327315/1‑126 (MQ=255)
                                                                                                    ttCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAgc                                                                   <  2:327315/126‑1 (MQ=255)
                                                                                                    ttCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGcc                                           <  2:1112572/150‑1 (MQ=255)
                                                                                                    ttCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGcc                                           >  2:630768/1‑150 (MQ=255)
                                                                                                       aCGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCagtag                                                                               <  2:790251/111‑1 (MQ=255)
                                                                                                       aCGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCagtag                                                                               >  1:790251/1‑111 (MQ=255)
                                                                                                         gCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCa                                      >  1:779997/1‑150 (MQ=255)
                                                                                                         gCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCa                                      >  2:1249052/1‑150 (MQ=255)
                                                                                                               tCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCt                                <  1:902466/150‑1 (MQ=255)
                                                                                                                 aCGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGcc                                           <  2:1286309/137‑1 (MQ=255)
                                                                                                                 aCGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGcc                                           >  1:1286309/1‑137 (MQ=255)
                                                                                                                  cGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGcc                                                                                               >  1:717195/1‑84 (MQ=255)
                                                                                                                  cGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGcc                                                                                               <  2:717195/84‑1 (MQ=255)
                                                                                                                    gTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTACCAGCCCGGTAAGAGAGCTCACCTGCTGCCCGCTGCACTc                                                                                     >  2:891588/1‑92 (MQ=255)
                                                                                                                    gTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTACCAGCCCGGTAAGAGAGCTCACCTGCTGCCCGCTGCACTc                                                                                     <  1:891588/92‑1 (MQ=255)
                                                                                                                     tAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGcccc                          >  2:488690/1‑149 (MQ=255)
                                                                                                                         aG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCACTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATAc                      >  2:752778/1‑150 (MQ=255)
                                                                                                                          g‑gCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCCGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATAcc                     <  2:861213/150‑1 (MQ=255)
                                                                                                                          g‑gCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATAcc                     <  1:1035972/150‑1 (MQ=255)
                                                                                                                                     ccACCGGGCAATGCCAGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTg             <  1:433562/148‑1 (MQ=255)
                                                                                                                                     ccACCGGGCAATGCCAGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTg             >  2:813126/1‑148 (MQ=255)
                                                                                                                                     ccACCGGGCAATGCCAGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTg             >  2:433562/1‑148 (MQ=255)
                                                                                                                                     ccACCGGGCAATGCCAGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTg             <  1:813126/148‑1 (MQ=255)
                                                                                                                                         cTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGTCCGa       <  1:945582/150‑1 (MQ=255)
                                                                                                                                          tGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCCACCTGGTCCGAg      >  2:1086873/1‑150 (MQ=255)
                                                                                                                                           ggCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGTCCGAGc     <  1:762321/150‑1 (MQ=255)
                                                                                                                                              aTTGCTCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGTCCGAGCCTa  >  2:239296/1‑150 (MQ=255)
                                                                                                                                              aTTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCACTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGTCCGAGCCTa  <  1:752778/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
TCTGGAACAAGCTCACCCCCAGCCCCTCCGCCTTGAAACTCCCTGCGCAATCCAGCGGCTGTTCTGCTGCCACGTAACGCTCGACCTGCTCCCTGCTCAGTTCACGCAGTGTCACGGTAAAAG‑GCACGCAATCCACCTGGCATTGCCCGGTGGCGCTGTTCAGCAGTGCCAGCCCGGTAAGAAAGCTCACCTGCTGCCCGCTGCACTCCAGTAGTTGCTCGCAAGCGCGCTCGAACGTATGCGGCTTGCCCAGCACCTGCTCGCCCAATACCGCAACCTGGTCCGAGCCTA  >  NC_002947/2154292‑2154582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑