Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,275,080 G→T 5.0% H151Q (CAC→CAA)  PP_2005 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,275,0800GT 5.0% 251.6 / 2.3 80H151Q (CAC→CAA) PP_2005hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (38/38);  new base T (2/2);  total (40/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.22e-01

GGCCGGTATGCAGTACGCCTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCCGCGCGCCCACAGCCAGGT  >  NC_002947/2274938‑2275229
                                                                                                                                              |                                                                                                                                                     
ggCCGGTATGCGGTACGCCTGGTTTCACGTAAGGTTCGGGCAGCACGATCGGACCGGCCGCCAAGGTGACGCGAAGTGCCCTGGCCAGGCCGGCGGCGAGCTGCTGGTAGTCTGCGGTCATATGCGCGGCCTTCATGCGCAAGTGCGCAt                                                                                                                                                <  1:304369/150‑1 (MQ=255)
 gCCGGTATGCAGTACGCCTTGTTTCACATAAGGTTCGTGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATc                                                                                                                                               >  2:881047/1‑150 (MQ=255)
    ggTATGCAGTACGCCTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGgc                                                                                                                                            >  1:882729/1‑150 (MQ=255)
           aGTACGCCTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAg                                                                                                                                     >  1:724077/1‑150 (MQ=255)
            gTACGCCTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAgt                                                                                                                                    >  1:194451/1‑150 (MQ=255)
                  cTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGc                                                                                                                              >  1:84271/1‑150 (MQ=255)
                        tCACATACGATTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAg                                                                                                                        >  1:495669/1‑150 (MQ=255)
                        tCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCATCGCCGTCAg                                                                                                                        >  1:760577/1‑150 (MQ=255)
                         cacaTAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAgc                                                                                                                       >  2:61568/1‑150 (MQ=255)
                          acaTAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAgcg                                                                                                                      >  2:1119614/1‑150 (MQ=255)
                           caTAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGCCGTCGCCGTCAgcgc                                                                                                                     >  2:894558/1‑150 (MQ=255)
                           caTAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTCCGCGCCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAgcgc                                                                                                                     >  1:1244907/1‑150 (MQ=255)
                                  ttCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAAc                                                                                                              <  1:1262038/150‑1 (MQ=255)
                                  ttCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAAc                                                                                                              >  1:384256/1‑150 (MQ=255)
                                         agcaCGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCATCGCCGTCAGCGCCAAGAACCGCGAgg                                                                                                       <  2:760577/150‑1 (MQ=255)
                                              gATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATa                                                                                                  >  2:1285970/1‑150 (MQ=255)
                                                tCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAgc                                                                                                >  1:176341/1‑150 (MQ=255)
                                                           accaAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAAc                                                                                                              >  2:170326/1‑125 (MQ=255)
                                                           accaAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAAc                                                                                                              <  1:170326/125‑1 (MQ=255)
                                                            ccaAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATa                                                                                                  <  2:538779/136‑1 (MQ=255)
                                                            ccaAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATa                                                                                                  >  1:538779/1‑136 (MQ=255)
                                                            ccaAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGc                                                                                    >  1:508993/1‑150 (MQ=255)
                                                              aaGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGCCCCTTCTCATCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTc                                                                                  <  2:369044/150‑1 (MQ=255)
                                                                  tGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCTGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGtt                                                                              <  2:833820/150‑1 (MQ=255)
                                                                     cGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCa                                                                           <  2:849131/150‑1 (MQ=255)
                                                                     cGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCa                                                                           <  2:176341/150‑1 (MQ=255)
                                                                     cGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAACGGGTTGCTCAGTTcct                                                                           <  2:882729/150‑2 (MQ=255)
                                                                               ccTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAg                                                                                >  2:1094107/1‑135 (MQ=255)
                                                                               ccTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAg                                                                                <  1:1094107/135‑1 (MQ=255)
                                                                                cTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCCCCAAGATCCGCGAGGCGATACCGCAGCGTGTTGCTCAGTTCCAGCCATTGTCgg                                                                <  1:161332/150‑1 (MQ=255)
                                                                                  ggCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGc                                                              <  2:307491/150‑1 (MQ=255)
                                                                                     cAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTc                                                           <  2:724077/150‑1 (MQ=255)
                                                                                       ggCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGt                                                         <  2:151924/150‑1 (MQ=255)
                                                                                          cgccgAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGTTCAGTTCCAGCCATTGTCGGGCTTCGTGGc                                                      <  1:881047/150‑1 (MQ=255)
                                                                                          cgccgAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGc                                                      >  1:533039/1‑150 (MQ=255)
                                                                                           gccgAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGc                                                                                           <  1:1111454/112‑1 (MQ=255)
                                                                                           gccgAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGc                                                                                           >  2:1111454/1‑112 (MQ=255)
                                                                                           gccgAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCt                                                     >  1:1091422/1‑150 (MQ=255)
                                                                                           gccgAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCt                                                     >  1:124568/1‑150 (MQ=255)
                                                                                               aaCAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTgg                                                 <  2:151660/150‑1 (MQ=255)
                                                                                               aaCAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTgg                                                 <  2:194451/150‑1 (MQ=255)
                                                                                                  aGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTg                                              >  1:924824/1‑150 (MQ=255)
                                                                                                    ctgctgGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGa                                             >  2:883030/1‑149 (MQ=255)
                                                                                                    ctgctgGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGa                                             <  1:883030/149‑1 (MQ=255)
                                                                                                    ctgctgGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGAc                                            <  2:508993/150‑1 (MQ=255)
                                                                                                      gctgGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGATTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACtt                                          >  1:446884/1‑150 (MQ=255)
                                                                                                       ctgGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTg                                         >  2:1169976/1‑150 (MQ=255)
                                                                                                          gTAGTCTGCGGCCCTCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATa                                      >  1:82882/1‑150 (MQ=255)
                                                                                                          gTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGTGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCttt                                                   >  1:1258209/1‑137 (MQ=255)
                                                                                                          gTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGTGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCttt                                                   <  2:1258209/137‑1 (MQ=255)
                                                                                                          gTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCggg                                                               >  2:329608/1‑125 (MQ=255)
                                                                                                          gTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCggg                                                               <  1:329608/125‑1 (MQ=255)
                                                                                                          gTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATa                                      <  2:384256/150‑1 (MQ=255)
                                                                                                             gTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCCTAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAg                                   <  2:924824/150‑1 (MQ=255)
                                                                                                                   ggCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGtt                             <  1:61568/150‑1 (MQ=255)
                                                                                                                    gCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGttc                            <  1:1169976/150‑1 (MQ=255)
                                                                                                                    gCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTCCCTTGATACCGCTCGtgc                            <  2:1244907/150‑3 (MQ=255)
                                                                                                                     ccATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGCACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGttct                           >  2:1252592/1‑150 (MQ=255)
                                                                                                                       aTCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGttcttg                         >  1:755174/1‑149 (MQ=255)
                                                                                                                         cTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGATTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTtctc                       <  2:446884/150‑1 (MQ=255)
                                                                                                                           gCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGa                                        >  2:1226319/1‑131 (MQ=255)
                                                                                                                           gCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGa                                        <  1:1226319/131‑1 (MQ=255)
                                                                                                                            cTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGt                                               >  1:776174/1‑123 (MQ=255)
                                                                                                                            cTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGt                                               <  2:776174/123‑1 (MQ=255)
                                                                                                                             tCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACt                                           >  1:298636/1‑126 (MQ=255)
                                                                                                                             tCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACt                                           <  2:298636/126‑1 (MQ=255)
                                                                                                                             tCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCcg                   >  1:262803/1‑150 (MQ=255)
                                                                                                                              cGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGGACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGa                                        <  1:497877/128‑1 (MQ=255)
                                                                                                                              cGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGa                                        >  2:497877/1‑128 (MQ=255)
                                                                                                                               ggCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGt                                                         <  2:156046/110‑1 (MQ=255)
                                                                                                                               ggCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGAAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGt                                                         >  1:156046/1‑110 (MQ=255)
                                                                                                                                    tCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTTGGTGACTTGATACAGCTCGTTCTTCTCTCCGCGCGccc            >  1:139300/1‑150 (MQ=255)
                                                                                                                                      aTGCGCACTTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGa                                        >  1:310508/1‑120 (MQ=255)
                                                                                                                                      aTGCGCACTTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGa                                        <  2:310508/120‑1 (MQ=255)
                                                                                                                                      aTGCGCACTTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCCgcgcgc              >  1:484248/1‑146 (MQ=255)
                                                                                                                                      aTGCGCACTTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCCgcgcgc              <  2:484248/146‑1 (MQ=255)
                                                                                                                                          gcAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCCGCGCGCCCACAGcc      <  1:658081/150‑1 (MQ=255)
                                                                                                                                          gcAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCCGCGCGCCCACAGcc      <  1:894558/150‑1 (MQ=255)
                                                                                                                                           cAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCCTCGCGCCCACAGCCa     <  2:755174/150‑1 (MQ=255)
                                                                                                                                           cAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCCGCGCGCCCACAGCCa     <  2:533039/150‑1 (MQ=255)
                                                                                                                                              gTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCCGCGCGCCCACAGCCAGGt  <  1:260162/150‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                     
GGCCGGTATGCAGTACGCCTTGTTTCACATAAGGTTCGGGCAGCACGATCGAACCGGCCACCAAGGTGACGCCAAATTCCCTGGCCAGGCCGCCGAACAGCTGCTGGTAGTCTGCGGCCATCTGCTCGGCCTTCATGCGCAAGTGCGCATCGGCGCGCCAGTCGTCGCCGTCAGCGCCAAGAACCGCGAGGCCATAGCGCAGCGGGTTGCTCAGTTCCAGCCATTGTCGGGCTTCGTGGCTTTGGGTGACTTGATACAGCTCGTTCTTCTCTCCGCGCGCCCACAGCCAGGT  >  NC_002947/2274938‑2275229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑