Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,326,466 C→A 9.9% R294M (AGG→ATG)  PP_2045 ← metallo‑beta‑lactamase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,326,4660CA 9.9% 118.3 / 2.2 40R294M (AGG→ATG) PP_2045metallo‑beta‑lactamase family protein
Reads supporting (aligned to +/- strand):  ref base C (19/17);  new base A (2/2);  total (21/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

GGGCGAACACCACCTCGGCCTGCTCGCCGTAGCGCACCAGCGATTGGTCCAGGTAATGGGCCCACACTTTCGGATCGCGCACCAGGGCCCCGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGCTGGAACTCCACCTCCACGCCGCTCAGGGTCATGCGTTGCAGCGGTTGGCTGATTTCGAGCGTCGGCGCGATGAGGCTGACAGTGGCATTGGC  >  NC_002947/2326317‑2326614
                                                                                                                                                     |                                                                                                                                                    
gggCGAACACCACCTCGGCCTGCTCGCCGTAGCGCACCAGCGATTGGTCCAGGTAATGGGCCCACACTTTCGGATCGCGCACCAGGGCCCCGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGccc                                                                                                                                                      >  2:514028/1‑150 (MQ=255)
       caccacCTCGGCCTGCTCGCCGTAGCGCACCAGCGATTGGTCCAGGTAATGGGCCCACACTTTCGGATCGCGCACCAGGGCCCCGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAgcgc                                                                                                                                               >  1:196593/1‑150 (MQ=255)
         ccaccTCGGCCTGCTCGCCGTAGCGCACCAGCGATTGGTCCAGGTAATGGGCCCACACTTTCGGATCGCGCACCAGGGCCCCGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGCCATGAGCGCCg                                                                                                                                             >  2:919305/1‑150 (MQ=255)
                  ccTGCTCGCCGTAGCGCACCAGCGATTGGTCCAGGTAATGGGCCAACACTTTCGGATCGCGCACCAGGGCCCCGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGCCATGAGCGCCGGGaaataaa                                                                                                                                    >  2:174071/1‑150 (MQ=255)
                   cTGCTCGCCGTAGCGCACCAGCGATTGGTCCAGGTAATGGGCCCACACTTTCGGATCGCGCACCAGGGCCCCGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAGCGCCGGGaaataaat                                                                                                                                   <  2:525457/150‑1 (MQ=255)
                       gcgccgcAGCGCACCAGCGATTGGTCCAGGTAATGGGCCCACACTTTCGGATCGCGCACCAGGGCCCCGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAGCGCCGGGAAATAAATGTTc                                                                                                                               <  2:5309/149‑1 (MQ=255)
                           cGTAGCGCACCAGCGATTGGTCCAGGTAATGGGCCCACACTTTCGGATCGCGCACCAGGGCCCCGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCAttt                                                                                                                           >  1:178319/1‑150 (MQ=255)
                            gTAGCGCACCAGCGATTGGTCCAGGTAATGGGCCCACACTTTCGGATCGCGCACCAGGGCCCCGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTc                                                                                                                          >  1:253335/1‑150 (MQ=255)
                                             ggTCCAGGTAATGGGCCCACACTTTCGGATCGCGCACCAGGGCCCCGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGc                                                                                                         <  2:253335/150‑1 (MQ=255)
                                              gTCCAGGTAATGGGCCCACACTTTCGGATCGCGCACAAGGGCCCCGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGcc                                                                                                        >  1:853403/1‑150 (MQ=255)
                                               tCCAGGTAATGGGCCCACACTTTCGGATCGCGCACCAGGGCCCCGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGccc                                                                                                       >  1:143015/1‑150 (MQ=255)
                                                 cAGGTAATGGGCCCACACTTTCGGATCGCGCACCAGGGCCCCGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCgg                                                                                                     <  1:885557/150‑1 (MQ=255)
                                                         gggCCCACACTTTCGGATCGCGCACCAGGGCCCCGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAGCGCCGGGAAATACATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGCt                                                                                             <  1:1225908/150‑1 (MQ=255)
                                                             ccACACTTTCGGATCGCGCACCAGGGCCCCGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGCTGGaa                                                                                         >  1:1066274/1‑150 (MQ=255)
                                                             ccACACTTTCGGATCGCGCACCAGGGCCCCGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGCTGGaa                                                                                         >  1:444915/1‑150 (MQ=255)
                                                              cacaCTTTCGGATCGCGCACCAGGGCCCCGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGCTGGAAc                                                                                        <  2:147710/150‑1 (MQ=255)
                                                                cacTTTCGGATCGCGCACCAGGGCCCCGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGCTGGAActc                                                                                      <  2:1066274/150‑1 (MQ=255)
                                                                   tttCGGATCGCGCACCAGGGCCCCGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGCTGGAActccac                                                                                   <  2:196593/150‑1 (MQ=255)
                                                                        gATCGCGCACCAGGGCCCCGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGCTGGAActccacctcca                                                                              <  2:143015/150‑1 (MQ=255)
                                                                         aTCGCGCACCAGGGCCCCGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGCTGGAActccacctccac                                                                             >  1:434431/1‑150 (MQ=255)
                                                                          tCGCGCACCAGGGCCCCGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGccc                                                                                                                                                      >  1:47519/1‑76 (MQ=255)
                                                                          tCGCGCACCAGGGCCCCGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGccc                                                                                                                                                      <  2:47519/76‑1 (MQ=255)
                                                                           cgcgCACCAGGGCCCCGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGCCCTCAGCGCCGGGAAATAAATGTTCAATTCCGAAGGCGCCTCGGTGCCCGGGGTCAGCTGGAACTCCACCTCCAcgc                                                                           <  2:1144830/150‑1 (MQ=255)
                                                                                    gggCCCCGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGCTGGAACTCCACCTCCACGCCGCTCAggg                                                                  >  2:1086561/1‑150 (MQ=255)
                                                                                      gCCCCGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGCTGGAACTCCACCTCCACGCCGCTCAGGGTc                                                                >  1:432034/1‑150 (MQ=255)
                                                                                      accccGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGCTGGAACTCCACCTCCACGCCGCTCAGGGTc                                                                >  2:197648/2‑150 (MQ=255)
                                                                                        cccGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGCTGGAACTCCACCTCCACGCCGCTCAGGGTCAt                                                              <  2:434431/150‑1 (MQ=255)
                                                                                         ccGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGCTGGAACTCCACCTCCACGCCGCTCAGGGTCATg                                                             >  1:509124/1‑150 (MQ=255)
                                                                                              cAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGCCATGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGCTGGAACTCCACCTCCACGCCGCTCAGGGTCATGCGTTg                                                        <  1:174071/150‑1 (MQ=255)
                                                                                                          gTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGCTGGAACTCCACCTCCACGCCGCTCAGGGTCATGCGTTGCAGCGGTTGGCt                                            >  1:277939/1‑150 (MQ=255)
                                                                                                                 tgcACATGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGCTGGAACTCCACCTCCACGCCGCTCAGGGTCATGCGTTGCAGCGGTTGGCTGATTTCg                                     >  2:59818/1‑150 (MQ=255)
                                                                                                                  gcACATGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGCTGGAACTCCACCTCCACGCCGCTCAGGGTCATGCGTTGCAGCGGTTGGCTGATTTCGa                                    <  1:1086561/150‑1 (MQ=255)
                                                                                                                   cacaTGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGCTGGAACTCCACCTCCACGCCGCTCAGGGTCATGCGTTGCAGCGGTTGGCTGATTTCGAg                                   <  1:514028/150‑1 (MQ=255)
                                                                                                                    acaTGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGc                                                                                              <  1:432748/90‑1 (MQ=255)
                                                                                                                    acaTGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGc                                                                                              >  2:432748/1‑90 (MQ=255)
                                                                                                                                     ggCCATGCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGCTGGAACTCCACCTCCACGCCGCTCAGGGTCATGCGTTGCAGCGGTTTGCTGATTTCAAGCGTCGGCTCTATGAGGCt                 >  2:67161/1‑150 (MQ=255)
                                                                                                                                     ggCCATGCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGCTGGAACTCCACCTCCACGCCGCTCAGGGTCATGCGTTGCAGCGGTTGGCTGATTTCGAGCGTCGGCGCGATGAGGCt                 <  1:59818/150‑1 (MQ=255)
                                                                                                                                         aTGCACAGTGCCATGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGCTGGAACTCCACCTCCACGCCGCTCAGGGTCATGCGTTGCAGCGGTTGGCTGATTTCGAGCGTCGGCGCGATGAGGCTGACa             <  1:919305/150‑1 (MQ=255)
                                                                                                                                           gCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGCTGGAACTCCACCTCCACGCCGCTCAGGGTCATGCGTTGCAGCGGTTGGCTGATTTCGAGCGTCGGCGCGa                        >  2:1027105/1‑137 (MQ=255)
                                                                                                                                           gCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGCTGGAACTCCACCTCCACGCCGCTCAGGGTCATGCGTTGCAGCGGTTGGCTGATTTCGAGCGTCGGCGCGa                        <  1:1027105/137‑1 (MQ=255)
                                                                                                                                            cacaGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGCTGGAACTCCACCTCCACGCCGCTCAGGGTCATGCGTTGCAGCGGTTGGCTGATTTCGAGCGTCGGCGCGATGAGGCTGACAGTg          <  1:629046/150‑1 (MQ=255)
                                                                                                                                               tgTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGCTGGAACTCCACCTCCACGCCGCTCAGTGTCATGCGTTGCAGCGGTTGGCTGATTTCGAGCGTCGGCGCGATGAGGCTGACAGTGGCa       >  2:308668/2‑150 (MQ=255)
                                                                                                                                                 tGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGCTGGAACTCCACCTCCACGCCGCTCAGGGTCATGCGTTGCAGCGGTTGGCTGATTTCGAGCGTCGGCGCGATGAGGCTGCCAGAGGCAtt     >  2:139137/1‑150 (MQ=255)
                                                                                                                                                    ccTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGCTGGAACTCCACCTCCACGCCGCTCAGGGTCATGCGTTGCAGCGGTTGGCTGATTTCGAGCGTCGGCGCGATGAGGCTGACAGTGGCATTGGc  >  1:711884/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GGGCGAACACCACCTCGGCCTGCTCGCCGTAGCGCACCAGCGATTGGTCCAGGTAATGGGCCCACACTTTCGGATCGCGCACCAGGGCCCCGCGCAGCGTCAGTACGTTGTGCTGCACATGGGTGGCGTTCTCGGCCATGCACAGTGCCCTGAGCGCCGGGAAATAAATGTTCATTTCCGCCGGCGCCTCGGTGCCCGGGGTCAGCTGGAACTCCACCTCCACGCCGCTCAGGGTCATGCGTTGCAGCGGTTGGCTGATTTCGAGCGTCGGCGCGATGAGGCTGACAGTGGCATTGGC  >  NC_002947/2326317‑2326614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑