Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,335,490 T→C 14.0% E337G (GAG→GGG)  PP_2052 ← bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,335,4900TC14.0% 97.9 / 8.1 43E337G (GAG→GGG) PP_2052bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (21/16);  new base C (4/2);  total (25/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.30e-01

GACAACTCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGA  >  NC_002947/2335343‑2335637
                                                                                                                                                   |                                                                                                                                                   
gACAACTCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCg                                                                                                                                                   >  2:1175481/1‑150 (MQ=255)
 aCAACTCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCgg                                                                                                                                                  <  1:125697/150‑1 (MQ=255)
  cAACTCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCggg                                                                                                                                                 <  1:1175481/150‑1 (MQ=255)
  cAACTCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCggg                                                                                                                                                 >  2:699184/1‑150 (MQ=255)
      tCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAgg                                                                                                                                             <  2:112932/150‑1 (MQ=255)
               acaTGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCg                                                                                                                                    >  1:856551/1‑150 (MQ=255)
               acaTGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCg                                                                                                                                    >  2:807259/1‑150 (MQ=255)
                         gcACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCgg                                                                                                                          <  2:856551/150‑1 (MQ=255)
                                        cGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGGAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCTGGCAGGCTCAGTCCGATGATTTCGGCAGTGGTGTACATGc                                                                                                           <  1:1226964/150‑1 (MQ=255)
                                           cAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCtga                                                                                                        <  1:1116587/150‑1 (MQ=255)
                                            aaCCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCtgat                                                                                                       <  1:807259/150‑1 (MQ=255)
                                            aaCCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCtgat                                                                                                       >  1:869080/1‑150 (MQ=255)
                                               cTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCCCGAGCAGGccatc                                                                                                                                        >  1:316825/1‑110 (MQ=255)
                                               cTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCCCGAGCAGGccatc                                                                                                                                        <  2:316825/114‑5 (MQ=255)
                                                         aCGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCCCGAGCAGGccat                                                                                                                                         >  2:180470/1‑100 (MQ=255)
                                                         aCGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCCCGAGCAGGccat                                                                                                                                         <  1:180470/103‑4 (MQ=255)
                                                           gATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGcgtcgtcgtc                                                                                        >  1:664406/1‑150 (MQ=255)
                                                                      tCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTTATGACCGCGTCGTCGTCGTCCATGTCGa                                                                             >  2:909243/1‑150 (MQ=255)
                                                                       cAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGAt                                                                            <  2:664406/150‑1 (MQ=255)
                                                                           tCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATg                                                                        >  1:633023/1‑150 (MQ=255)
                                                                                 ccTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACg                                                                  >  1:878043/1‑150 (MQ=255)
                                                                                  cTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGt                                                                 >  1:912254/1‑150 (MQ=255)
                                                                                                  ttGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGAtggtctggtc                                                 >  2:244761/1‑150 (MQ=255)
                                                                                                     cagcagGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGaa                                              <  1:699184/150‑1 (MQ=255)
                                                                                                      agcagGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACAt                                                                                                             <  2:145343/86‑1 (MQ=255)
                                                                                                      agcagGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACAt                                                                                                             >  1:145343/1‑86 (MQ=255)
                                                                                                            ccTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGtt                                       <  2:878043/150‑2 (MQ=255)
                                                                                                                      tCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAgc                             >  1:101899/1‑150 (MQ=255)
                                                                                                                        aCCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAgcgc                           >  1:279555/1‑150 (MQ=255)
                                                                                                                         gccgttcctggcTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCa                          >  1:685647/7‑150 (MQ=255)
                                                                                                                         cccTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCgacagcag                          >  2:1228529/1‑143 (MQ=255)
                                                                                                                         cccTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCa                          >  2:836749/1‑150 (MQ=255)
                                                                                                                             ggcctggcTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCt                      <  2:101899/150‑1 (MQ=255)
                                                                                                                                ctggcTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTa                   >  2:1225649/1‑150 (MQ=255)
                                                                                                                                  ggcTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGc                 <  2:912254/150‑1 (MQ=255)
                                                                                                                                     ttCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGc              >  2:839006/1‑150 (MQ=255)
                                                                                                                                        cctgcgCCTGCCCGTGCGGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGa           >  2:921901/6‑150 (MQ=255)
                                                                                                                                        cctgagCCTGCCCGAGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGa           >  1:657192/6‑150 (MQ=255)
                                                                                                                                              ccTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGgcgc     >  2:431311/1‑150 (MQ=255)
                                                                                                                                               cTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTc                                                             >  1:563911/1‑93 (MQ=255)
                                                                                                                                               cTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTc                                                             <  2:563911/93‑1 (MQ=255)
                                                                                                                                               cTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCt    <  1:839006/150‑1 (MQ=255)
                                                                                                                                                 gCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGa  <  1:1207208/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GACAACTCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGA  >  NC_002947/2335343‑2335637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑