Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,335,493 G→A 13.0% P336L (CCC→CTC)  PP_2052 ← bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,335,4930GA13.0% 85.5 / 6.4 39P336L (CCC→CTC) PP_2052bifunctional sugar‑phosphatase/mannitol‑1‑phosphate 5‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (19/14);  new base A (3/2);  total (23/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.81e-01

ACAACTCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCT  >  NC_002947/2335344‑2335641
                                                                                                                                                     |                                                                                                                                                    
aCAACTCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCgg                                                                                                                                                      <  1:125697/150‑1 (MQ=255)
 cAACTCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCggg                                                                                                                                                     <  1:1175481/150‑1 (MQ=255)
 cAACTCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCggg                                                                                                                                                     >  2:699184/1‑150 (MQ=255)
     tCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAgg                                                                                                                                                 <  2:112932/150‑1 (MQ=255)
              acaTGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCg                                                                                                                                        >  1:856551/1‑150 (MQ=255)
              acaTGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCg                                                                                                                                        >  2:807259/1‑150 (MQ=255)
                        gcACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCgg                                                                                                                              <  2:856551/150‑1 (MQ=255)
                                       cGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGGAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCTGGCAGGCTCAGTCCGATGATTTCGGCAGTGGTGTACATGc                                                                                                               <  1:1226964/150‑1 (MQ=255)
                                          cAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCtga                                                                                                            <  1:1116587/150‑1 (MQ=255)
                                           aaCCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCtgat                                                                                                           <  1:807259/150‑1 (MQ=255)
                                           aaCCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCtgat                                                                                                           >  1:869080/1‑150 (MQ=255)
                                              cTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCCCGAGCAGGccatc                                                                                                                                            >  1:316825/1‑110 (MQ=255)
                                              cTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCCCGAGCAGGccatc                                                                                                                                            <  2:316825/114‑5 (MQ=255)
                                                        aCGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCCCGAGCAGGccat                                                                                                                                             <  1:180470/103‑4 (MQ=255)
                                                        aCGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCCCGAGCAGGccat                                                                                                                                             >  2:180470/1‑100 (MQ=255)
                                                          gATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGcgtcgtcgtc                                                                                            >  1:664406/1‑150 (MQ=255)
                                                                     tCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTTATGACCGCGTCGTCGTCGTCCATGTCGa                                                                                 >  2:909243/1‑150 (MQ=255)
                                                                      cAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGAt                                                                                <  2:664406/150‑1 (MQ=255)
                                                                          tCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATg                                                                            >  1:633023/1‑150 (MQ=255)
                                                                                ccTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACg                                                                      >  1:878043/1‑150 (MQ=255)
                                                                                 cTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGt                                                                     >  1:912254/1‑150 (MQ=255)
                                                                                                 ttGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGAtggtctggtc                                                     >  2:244761/1‑150 (MQ=255)
                                                                                                    cagcagGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGaa                                                  <  1:699184/150‑1 (MQ=255)
                                                                                                     agcagGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACAt                                                                                                                 <  2:145343/86‑1 (MQ=255)
                                                                                                     agcagGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACAt                                                                                                                 >  1:145343/1‑86 (MQ=255)
                                                                                                           ccTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGtt                                           <  2:878043/150‑2 (MQ=255)
                                                                                                                     tCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAgc                                 >  1:101899/1‑150 (MQ=255)
                                                                                                                       aCCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAgcgc                               >  1:279555/1‑150 (MQ=255)
                                                                                                                        gccgttcctggcTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCa                              >  1:685647/7‑150 (MQ=255)
                                                                                                                        cccTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCgacagcag                              >  2:1228529/1‑143 (MQ=255)
                                                                                                                        cccTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCa                              >  2:836749/1‑150 (MQ=255)
                                                                                                                            ggcctggcTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCt                          <  2:101899/150‑1 (MQ=255)
                                                                                                                               ctggcTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTa                       >  2:1225649/1‑150 (MQ=255)
                                                                                                                                 ggcTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGc                     <  2:912254/150‑1 (MQ=255)
                                                                                                                                    ttCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGc                  >  2:839006/1‑150 (MQ=255)
                                                                                                                                       cctgcgCCTGCCCGTGCGGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGa               >  2:921901/6‑150 (MQ=255)
                                                                                                                                       cctgagCCTGCCCGAGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGa               >  1:657192/6‑150 (MQ=255)
                                                                                                                                             ccTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGgcgc         >  2:431311/1‑150 (MQ=255)
                                                                                                                                              cTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTc                                                                 >  1:563911/1‑93 (MQ=255)
                                                                                                                                              cTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTc                                                                 <  2:563911/93‑1 (MQ=255)
                                                                                                                                              cTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCt        <  1:839006/150‑1 (MQ=255)
                                                                                                                                                gCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGa      <  1:1207208/150‑1 (MQ=255)
                                                                                                                                                    gggCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCt  <  2:751122/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ACAACTCGGCTTGCACATGACGCCGCACGAACGCTGCACCGCCAACCTTGTTCAGCACGATCAGCAGGGTCAGCTCGCGGCCTCGGCGCTCGAAGCGTTGCAGCAGGCCTTGGGCGATCACCCTGGCCTGGCTTCTGATGGCCTGCTCGGGCAGGCTCAGGCCGATGATTTCGGCAGTGGTGTACATGCTGATGACCGCGTCGTCGTCGTCCATGTCGATCATGCGCACGTTGTCGATGGTCTGGTCGAACGACGTGGCACCGTAGCGCACGCTGTAGCGGCCGAAGGCGCTGACGCT  >  NC_002947/2335344‑2335641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑