Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,347,661 G→C 12.4% V307L (GTG→CTG) ‡ PP_2064 → multidrug RND transporter membrane fusion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,347,6610GC12.4% 135.0 / 9.9 48V307L (GTG→CTG) ‡PP_2064multidrug RND transporter membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base G (28/14);  new base C (2/4);  total (30/18)
Fisher's exact test for biased strand distribution p-value = 1.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

CCGGGTATGTATGCCGGCGTGCGGCTGGATGCTGCGCAGCCGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCGTGGCGCAG  >  NC_002947/2347523‑2347801
                                                                                                                                          |                                                                                                                                            
ccGGGTATGTATGCCGGCGTGCGGCTGGATGCTGCGCAGCCGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGCTCACCCGGGTg                                                                                                                                   <  1:1181215/150‑1 (MQ=255)
ccGGGTATGTATGCCGGCGTGCGGCTGGATGCTGCGCAGCCGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGCTCACCCGGGTg                                                                                                                                   <  2:30970/150‑1 (MQ=255)
    gtatgtatGCCGGCGTGCGGCTGGATGCTGCGCAGCCGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAgg                                                                                                                               >  2:464312/1‑150 (MQ=255)
                          ggATGCTGCGCAGCCGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAg                                                                                                                 >  1:1177824/1‑142 (MQ=255)
                          ggATGCTGCGCAGCCGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAg                                                                                                                 <  2:1177824/142‑1 (MQ=255)
                             tgctgcGCAGCCGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAggg                                                                                                      >  2:678890/1‑150 (MQ=255)
                                 gcgcAGCCGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCgg                                                                                                  >  2:1095745/1‑150 (MQ=255)
                                       ccGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGaa                                                                                            <  1:464312/150‑1 (MQ=255)
                                         gTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTATCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAAt                                                                                          >  2:876112/1‑150 (MQ=255)
                                            gACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCAc                                                                                       <  1:31147/150‑1 (MQ=255)
                                                      gCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTg                                                                             >  1:1278703/1‑150 (MQ=255)
                                                         gTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACg                                                                          >  1:363796/1‑150 (MQ=255)
                                                              ggAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCgg                                                                     >  2:582824/1‑150 (MQ=255)
                                                                 aaCCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGa                                                                  >  1:77249/1‑150 (MQ=255)
                                                                   ccGCGATCACCTACACCGACTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGAcc                                                                <  2:297415/150‑1 (MQ=255)
                                                                         tCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGGAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTgtgg                                                          <  2:118429/150‑1 (MQ=255)
                                                                           aCCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAATCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTgtggtg                                                        <  2:627615/150‑1 (MQ=255)
                                                                           aCCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTgtggtg                                                        >  2:416163/1‑150 (MQ=255)
                                                                             cTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTggtggt                                                      >  2:1259675/1‑150 (MQ=255)
                                                                               acacCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGttt                                                    >  1:1027447/1‑150 (MQ=255)
                                                                                cacCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTc                                                   >  1:341338/1‑150 (MQ=255)
                                                                                   cGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGCTCACCCGGGTg                                                                                                                                   <  1:845538/67‑1 (MQ=38)
                                                                                   cGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGCTCACCCGGGTg                                                                                                                                   >  2:845538/1‑67 (MQ=38)
                                                                                    gCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGc                                               >  2:830102/1‑150 (MQ=255)
                                                                                      cTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCa                                             >  1:768366/1‑150 (MQ=255)
                                                                                            gCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTg                                         >  1:839090/1‑148 (MQ=255)
                                                                                            gCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTg                                         <  2:839090/148‑1 (MQ=255)
                                                                                                aCGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTg                                   >  1:1170329/1‑150 (MQ=255)
                                                                                                 cGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGCGATGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGa                                  >  1:404026/1‑150 (MQ=255)
                                                                                                  ggTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTAAGCGCTGGCAGGGGCGGGTGGAAATCACCTACGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAg                                 >  2:225234/1‑150 (MQ=255)
                                                                                                   gtgtTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGt                                <  2:363796/150‑1 (MQ=255)
                                                                                                       ttGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATg                            <  2:662583/150‑1 (MQ=255)
                                                                                                          tCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCa                         <  1:416163/150‑1 (MQ=255)
                                                                                                               aCCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCg                    >  2:682803/1‑150 (MQ=255)
                                                                                                                 ccAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGt                  >  2:395312/1‑150 (MQ=255)
                                                                                                                  cAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTc                 >  1:756149/1‑150 (MQ=255)
                                                                                                                         cGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGGTGGCATGCCGGTCGAGCCCg          <  2:77249/150‑1 (MQ=255)
                                                                                                                         cGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCg          >  2:1120340/1‑150 (MQ=255)
                                                                                                                         cGCAGCATGGCACCCGGGTGAGCCGGGNGCAGGTAACCACNGGTGAGNGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTTAAACTGAGTGATGGCATGCCGGTCGAGCTCg          >  1:970902/1‑150 (MQ=255)
                                                                                                                          gcagcaTGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCGt         >  1:1083682/1‑150 (MQ=255)
                                                                                                                           cagcaTGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCGTg        <  1:158179/150‑1 (MQ=255)
                                                                                                                              caTGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCGTGgcg     >  1:945905/1‑150 (MQ=255)
                                                                                                                               aTGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCGTGgcgc    <  1:1095745/150‑1 (MQ=255)
                                                                                                                                ctgCACCCGGCTCACCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGc                                                                                >  2:515673/3‑73 (MQ=37)
                                                                                                                                ctgCACCCGGCTCACCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGc                                                                                <  1:515673/71‑1 (MQ=37)
                                                                                                                                 ggCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGCGTGGAAATCACCTCCGCCCTGACGCCCGGTGACCGTGAGGTGGTTTCCGGCCCGCAGAGACTGAGTGATGGCATGCAGGTAGATCCCGTGGCTCat  >  2:596275/1‑149 (MQ=255)
                                                                                                                                    aCCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCGTgg       >  1:847484/1‑142 (MQ=255)
                                                                                                                                    aCCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCGTgg       <  2:847484/142‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                            
CCGGGTATGTATGCCGGCGTGCGGCTGGATGCTGCGCAGCCGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCGTGGCGCAG  >  NC_002947/2347523‑2347801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑