Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,347,665 G→C 12.3% S308T (AGC→ACC)  PP_2064 → multidrug RND transporter membrane fusion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,347,6650GC12.3% 130.0 / 11.5 49S308T (AGC→ACC) PP_2064multidrug RND transporter membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base G (28/15);  new base C (2/4);  total (30/19)
Fisher's exact test for biased strand distribution p-value = 1.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.02e-01

CCGGGTATGTATGCCGGCGTGCGGCTGGATGCTGCGCAGCCGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCGTGGCGCAGGACAGCCTGCAGG  >  NC_002947/2347523‑2347814
                                                                                                                                              |                                                                                                                                                     
ccGGGTATGTATGCCGGCGTGCGGCTGGATGCTGCGCAGCCGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGCTCACCCGGGTg                                                                                                                                                <  2:30970/150‑1 (MQ=255)
ccGGGTATGTATGCCGGCGTGCGGCTGGATGCTGCGCAGCCGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGCTCACCCGGGTg                                                                                                                                                <  1:1181215/150‑1 (MQ=255)
    gtatgtatGCCGGCGTGCGGCTGGATGCTGCGCAGCCGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAgg                                                                                                                                            >  2:464312/1‑150 (MQ=255)
                          ggATGCTGCGCAGCCGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAg                                                                                                                              >  1:1177824/1‑142 (MQ=255)
                          ggATGCTGCGCAGCCGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAg                                                                                                                              <  2:1177824/142‑1 (MQ=255)
                             tgctgcGCAGCCGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAggg                                                                                                                   >  2:678890/1‑150 (MQ=255)
                                 gcgcAGCCGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCgg                                                                                                               >  2:1095745/1‑150 (MQ=255)
                                       ccGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGaa                                                                                                         <  1:464312/150‑1 (MQ=255)
                                         gTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTATCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAAt                                                                                                       >  2:876112/1‑150 (MQ=255)
                                            gACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCAc                                                                                                    <  1:31147/150‑1 (MQ=255)
                                                      gCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTg                                                                                          >  1:1278703/1‑150 (MQ=255)
                                                         gTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACg                                                                                       >  1:363796/1‑150 (MQ=255)
                                                              ggAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCgg                                                                                  >  2:582824/1‑150 (MQ=255)
                                                                 aaCCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGa                                                                               >  1:77249/1‑150 (MQ=255)
                                                                   ccGCGATCACCTACACCGACTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGAcc                                                                             <  2:297415/150‑1 (MQ=255)
                                                                         tCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGGAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTgtgg                                                                       <  2:118429/150‑1 (MQ=255)
                                                                           aCCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAATCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTgtggtg                                                                     <  2:627615/150‑1 (MQ=255)
                                                                           aCCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTgtggtg                                                                     >  2:416163/1‑150 (MQ=255)
                                                                             cTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTggtggt                                                                   >  2:1259675/1‑150 (MQ=255)
                                                                               acacCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGttt                                                                 >  1:1027447/1‑150 (MQ=255)
                                                                                cacCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTc                                                                >  1:341338/1‑150 (MQ=255)
                                                                                   cGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGCTCACCCGGGTg                                                                                                                                                >  2:845538/1‑67 (MQ=38)
                                                                                   cGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGCTCACCCGGGTg                                                                                                                                                <  1:845538/67‑1 (MQ=38)
                                                                                    gCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGc                                                            >  2:830102/1‑150 (MQ=255)
                                                                                      cTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCa                                                          >  1:768366/1‑150 (MQ=255)
                                                                                            gCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTg                                                      <  2:839090/148‑1 (MQ=255)
                                                                                            gCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTg                                                      >  1:839090/1‑148 (MQ=255)
                                                                                                aCGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTg                                                >  1:1170329/1‑150 (MQ=255)
                                                                                                 cGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGCGATGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGa                                               >  1:404026/1‑150 (MQ=255)
                                                                                                  ggTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTAAGCGCTGGCAGGGGCGGGTGGAAATCACCTACGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAg                                              >  2:225234/1‑150 (MQ=255)
                                                                                                   gtgtTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGt                                             <  2:363796/150‑1 (MQ=255)
                                                                                                       ttGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATg                                         <  2:662583/150‑1 (MQ=255)
                                                                                                          tCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCa                                      <  1:416163/150‑1 (MQ=255)
                                                                                                               aCCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCg                                 >  2:682803/1‑150 (MQ=255)
                                                                                                                 ccAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGt                               >  2:395312/1‑150 (MQ=255)
                                                                                                                  cAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTc                              >  1:756149/1‑150 (MQ=255)
                                                                                                                         cGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGGTGGCATGCCGGTCGAGCCCg                       <  2:77249/150‑1 (MQ=255)
                                                                                                                         cGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCg                       >  2:1120340/1‑150 (MQ=255)
                                                                                                                         cGCAGCATGGCACCCGGGTGAGCCGGGNGCAGGTAACCACNGGTGAGNGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTTAAACTGAGTGATGGCATGCCGGTCGAGCTCg                       >  1:970902/1‑150 (MQ=255)
                                                                                                                          gcagcaTGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCGt                      >  1:1083682/1‑150 (MQ=255)
                                                                                                                           cagcaTGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCGTg                     <  1:158179/150‑1 (MQ=255)
                                                                                                                              caTGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCGTGgcg                  >  1:945905/1‑150 (MQ=255)
                                                                                                                               aTGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCGTGgcgc                 <  1:1095745/150‑1 (MQ=255)
                                                                                                                                ctgCACCCGGCTCACCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGc                                                                                             >  2:515673/3‑73 (MQ=37)
                                                                                                                                ctgCACCCGGCTCACCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGc                                                                                             <  1:515673/71‑1 (MQ=37)
                                                                                                                                 ggCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGCGTGGAAATCACCTCCGCCCTGACGCCCGGTGACCGTGAGGTGGTTTCCGGCCCGCAGAGACTGAGTGATGGCATGCAGGTAGATCCCGTGGCTCat               >  2:596275/1‑149 (MQ=255)
                                                                                                                                    aCCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCGTgg                    >  1:847484/1‑142 (MQ=255)
                                                                                                                                    aCCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCGTgg                    <  2:847484/142‑1 (MQ=255)
                                                                                                                                             aGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCGTGGCGCAGGACAGCCTGCAg   <  2:1170329/150‑1 (MQ=255)
                                                                                                                                              gCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCGTGGCGCAGGACAGCCTGCAgg  <  2:1027447/150‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                     
CCGGGTATGTATGCCGGCGTGCGGCTGGATGCTGCGCAGCCGTCGACCGTGCTGGCCGTGCCGGAAACCGCGATCACCTACACCGCCTATGGGCAGACGGTGTTTGTCGCGACCCAGGACCCGCAGCATGGCACCCGGGTGAGCCGGGTGCAGGTAACCACCGGTGAGCGCTGGCAGGGGCGGGTGGAAATCACCTCCGGCCTGACGCCCGGTGACCGTGTGGTGGTTTCCGGCCAGCTGAAACTGAGTGATGGCATGCCGGTCGAGCCCGTGGCGCAGGACAGCCTGCAGG  >  NC_002947/2347523‑2347814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑