Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,407,993 G→T 8.2% A205A (GCC→GCA)  PP_2111 ← aerotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,407,9930GT 8.2% 141.4 / 2.3 49A205A (GCC→GCA) PP_2111aerotaxis receptor
Reads supporting (aligned to +/- strand):  ref base G (26/19);  new base T (2/2);  total (28/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.50e-01

TTCGGCACTGTCTTGAAGACGCGTCAGGCAGGTTTTCATGCGTGCGTCCTGGCTGAGCATGGCCATTTCCAGGCGCGCCTGCACGCCACGGCTGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCAGACCCAGTGGTACCGAAAGCCCTGCGGCCAAGGCAAAGCCCCAGGAATGACCCAGCCAGTTGCCGATCAGGAAGCCCACCTGGCTGATCAGGAT  >  NC_002947/2407855‑2408139
                                                                                                                                          |                                                                                                                                                  
ttCGGCACTGTCTTGAAGACGCGTCAGGCAGGTTTTCATGCGTGCGTCCTGGCTGAGCATGGCCATTTCCAGGCGCGCCTGCACGCCACGGCTGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCgcag                                                                                                                                         >  1:211511/1‑150 (MQ=255)
  cGGCACTGTCTTGAAGACGCGTCAGGCAGGTTTTCATGCGTGCGGCCTGGCTGAGCATGGCCATTGCCAGGCGCGCCTGCACGCCACGGCTGTCTGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCgcagca                                                                                                                                       <  1:550818/150‑1 (MQ=255)
   ggCACTGTCTTGAAGACGCGTCAGGCAGGTTTTCATGCGTGCGTCCTGGCTGAGCATGGCCATTTCCAGGCGCGCCTGCACGCCACGGCTGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGcagcag                                                                                                                                      >  2:541758/1‑150 (MQ=255)
         gTCTTGAAGACGCGTCAGGCAGGTTTTCATGCGTGCGTCCTGGCTGAGCATGGCCATTTCCAGGCGCGCCTGCACGCCACGGCTGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCtt                                                                                                                                <  1:727023/150‑1 (MQ=255)
           cTTGAAGACGCGTCAGGCAGGTTTTCATGCGTGCGTCCTGGCTGAGCATGGCCATTTCCAGGCGCGCCTGCACGCCACGGCTGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGa                                                                                                                              <  2:472718/150‑1 (MQ=255)
            ttGAATACGCGTCGGGCAGGTTTTCATGCGTGCGTCCTGGCTGAGCATGGCCATTTCCAGGCGCGCCTGCACGCCACGGCTGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAg                                                                                                                             >  2:637718/1‑150 (MQ=255)
              gAAGACGCGTCAGGCAGGTTTTCATGCGTGCGTCCTGGCTGAGCATGGCCATTTCCAGGCGCGCCTGCACGCCACGGCTGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGc                                                                                                                           >  2:131837/1‑150 (MQ=255)
               aaGACGCGTCAGGCAGGTTTTCATGCGTGCGTCCTGGCTGAGCATGGCCATTTCCAGGCGCGCCTGCACGCCACGGCTGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGcc                                                                                                                          >  1:129920/1‑150 (MQ=255)
                    gcgTCAGGCAGGTTTTCATGCGTGCGTCCTGGCTGAGCATGGCCATTTCCAGGCGCGCCTGCACGCCACGGCTGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCgct                                                                                                                     >  1:742855/1‑150 (MQ=255)
                       tCAGGCAGGTTTTCATGCGTGCGTCCTGGCTGAGCATGGCCATTTCCAGGCGCGCCTGCACGCCACGGCTGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCgctgct                                                                                                                  <  1:131837/150‑1 (MQ=255)
                         attcaggTTTGCATGCGTGCGTCCTGGCTGAGCATGGCCATTTCCAGGCGCGCCTGCACGCCACGGCTGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCtgctgc                                                                                                                <  2:211511/147‑1 (MQ=255)
                         aggcaggTTTTCATGCGTGCGTCCTGGCTGAGCATGGCCATTTCCAGGCGCGCCTGCACGCCACGGCTGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCtgctgc                                                                                                                <  1:1101841/150‑1 (MQ=255)
                          ggcaggTTTTCATGCGTGCGTCCTGGCTGAGCATGGCCATTTCCAGGCGCGCCTGCACGCCACGGCTGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGcc                                                                                                               >  2:1011937/1‑150 (MQ=255)
                             aggTTTTCATGCGTGCGTCCTGGCTGAGCATGGCCATTTCCAGGCGCGCCTGCACGCCACGGCTGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGc                                                                                                            >  2:120807/1‑150 (MQ=255)
                               ggtttCATGCGTGCGTCCTGGCTGAGCATGGCCATTTCCAGGCGCGCCTGCACGCCACGGCTGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTc                                                                                                          >  2:371424/3‑150 (MQ=255)
                               gTTTTCATGCGTGCGTCCTGGCTGAGCATGGCCATTTCCAGGCGCGCCTGCACGCCACGGCTGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTc                                                                                                          >  2:826055/1‑150 (MQ=255)
                                tattaaTGCGTGCGTCCTGGTTGAGCATAGCGATTTTCAGACGTGCCTGCACGCCATTGTTGTCGGTGAACATCTGCGCGATCAGTGGGTAGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCa                                                                                                         >  2:487993/6‑150 (MQ=255)
                                       gcgtgcgtCCTGGCTGAGCATGGCCATTTCCAGGCGCGCCTGCACGCCACGGCTGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGAGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAg                                                                                                  >  1:16546/1‑150 (MQ=255)
                                             gtCCTGGCTGAGCATGGCCATTTCCAGGCGCGCCTGCACGCCACGGCTGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCAGAcc                                                                                            <  1:492862/150‑1 (MQ=255)
                                                     tGAGCATGGCCATTTCCAGGCGCGCCTGCACGCCACGGCTGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCAGACCCAGTGGTa                                                                                    >  2:959003/1‑150 (MQ=255)
                                                     tGAGCATGGCCATTTCCAGGCGCGCCTGCACGCCACGGCTGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCCCTGCTTCCAGCTCAGGCCAAGCAGACCCAGTGGTa                                                                                    >  2:1173409/1‑150 (MQ=255)
                                                      gAGCATGGCCATTTCCAGGCGCGCCTGCACGCCACGGCTGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCAGACCCAGTGGTAc                                                                                   <  1:541758/150‑1 (MQ=255)
                                                           tGGCCATTTCCAGGCGCGCCTGCACGCCACGGCTGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCAGACCCAGTGGTACCGaaa                                                                              >  1:217548/1‑150 (MQ=255)
                                                            ggCCATTTCCAGGCGCGCCTGCACGCCACGGCTGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGGGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAACAGACCCAGTGGTACCGAAAg                                                                             >  1:225259/1‑150 (MQ=255)
                                                                        gcgcgcCTGCACGCCACGGCTGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCAGACCCAGTGGTACCGAAAGCCCTGCGGCCaa                                                                 <  1:915920/150‑1 (MQ=255)
                                                                          gcgcCTGCACGCCACGGCTGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCAGACCCAGTGGTACCGAAAGCCCTGCGGCCAAgg                                                               <  1:1075200/150‑1 (MQ=255)
                                                                                    gCCACGGCTGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGATTTACTCGGCCAGTCTCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCAGACCCAGTGGTACCGAAAGCCCTGCGGCCAAGGCAAAGCCCCa                                                     <  1:1250161/150‑1 (MQ=255)
                                                                                         ggCTGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCAGACCCAGTGGTACCGAAAGCCCTGCGGCCAAGGCAAAGCCCCAGGAAt                                                <  1:959003/150‑1 (MQ=255)
                                                                                            tGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCAGACCCAGTGGTACCGAAAGCCCTGCGGCCAAGGCAAAGCCCCAGGAATGAc                                             >  2:563619/1‑150 (MQ=255)
                                                                                            tGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCAGACCCAGCGGTACCGAAAGCCCTGCGGCCAAGGCAAAGCCCCAGGAATGAc                                             >  2:150267/1‑150 (MQ=255)
                                                                                                 gTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCAGACCCAGt                                                                                        >  2:579425/1‑102 (MQ=255)
                                                                                                 gTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCAGACCCAGt                                                                                        <  1:579425/102‑1 (MQ=255)
                                                                                                         cTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCAGACCCAGTGGTACCGa                                                                                <  1:388166/102‑1 (MQ=255)
                                                                                                         cTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCAGACCCAGTGGTACCGa                                                                                >  2:388166/1‑102 (MQ=255)
                                                                                                          tGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCTGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCa                                                                                                <  1:833667/85‑1 (MQ=255)
                                                                                                          tGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCTGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCa                                                                                                <  1:833575/85‑1 (MQ=255)
                                                                                                          tGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCTGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCa                                                                                                >  2:833575/1‑85 (MQ=255)
                                                                                                          tGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCTGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCa                                                                                                >  2:833667/1‑85 (MQ=255)
                                                                                                              cgATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCAGACCCAGTGGTACCGAAAGCCCTGCGGCCAAGGCAAAGCCCCAGGAATGACCCAGCCAGTTGCCGATCa                           <  1:733396/150‑1 (MQ=255)
                                                                                                                aTCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCAGACCCAGTGGTACCGAAAGCCCTGCGGCCAAGGCAAAGCCCCAGGAATGACCCAGCCAGTTGCCGATCAgg                         >  1:430100/1‑150 (MQ=255)
                                                                                                                     tGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCAGACCCAGTGGTACCGAAAGCCCTGCGGCCAAGGCAAAGCCCCAGGAATGACCCAGCCAGTTGCCGATCAGGAAGcc                    >  2:329664/1‑150 (MQ=255)
                                                                                                                        gTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCAGACCCAGTGGTACCGAAAGCCCTGCGGCCAAGGCAAAGCCCCAGGAATGACCCAGCCAGTTGCCGATCAGGAAGCCCAc                 >  2:1274710/1‑150 (MQ=255)
                                                                                                                        gTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCAGACCCAGTGGTACCGAAAGCCCTGCGGCCAAGGCAAAGCCCCAGGAATGACCCAGCCAGTTGCCGATCAGGAAGCCCAc                 >  1:1259973/1‑150 (MQ=255)
                                                                                                                            gAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCAGACCCAGTGGTACCGAAAGCCCTGCGGCCAAGGc                                                              <  2:117803/101‑1 (MQ=255)
                                                                                                                            gAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCAGACCCAGTGGTACCGAAAGCCCTGCGGCCAAGGc                                                              >  1:117803/1‑101 (MQ=255)
                                                                                                                            gAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCAGACCCAGTGGTACCGAAAGCCCTGCGGCCAAGGCAAAGCCCCAGGAATGACCCAGCCAGTTGCCGATCAGGaa                       >  1:630986/1‑140 (MQ=255)
                                                                                                                            gAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCAGACCCAGTGGTACCGAAAGCCCTGCGGCCAAGGCAAAGCCCCAGGAATGACCCAGCCAGTTGCCGATCAGGaa                       <  2:630986/140‑1 (MQ=255)
                                                                                                                                     tGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCTAGCTCAGGCCAAGCAGACCCAGTGGTACCGAAAGCCCTGCGGCCAAGGCAAAGCCCCAGGAATGACCCAGCCAGTTGCCGATCAGGAAGCCCACCTGGCTGATCAgg    <  1:1173409/150‑1 (MQ=255)
                                                                                                                                       cTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCAGACCCAGTGGTACCGAAAGCCCTGCGGCCAAGGCAAAGCCCCAGGAATGACCCAGCCAGTTGCCGATCAGGAAGCCCACCTGGCTGATCAGGAt  <  1:637718/150‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                  
TTCGGCACTGTCTTGAAGACGCGTCAGGCAGGTTTTCATGCGTGCGTCCTGGCTGAGCATGGCCATTTCCAGGCGCGCCTGCACGCCACGGCTGTCGGTGAACATCTGCGCGATCAGTGGGTCGGAAGTGGTTTGCTCGGCCAGGCGCAGCAGGCGCTTGAGGCCGCGCTGCTGCCAGCTCAGGCCAAGCAGACCCAGTGGTACCGAAAGCCCTGCGGCCAAGGCAAAGCCCCAGGAATGACCCAGCCAGTTGCCGATCAGGAAGCCCACCTGGCTGATCAGGAT  >  NC_002947/2407855‑2408139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑