Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,439,333 G→T 8.3% V11F (GTC→TTC)  pcaF‑II → beta‑ketoadipyl‑CoA thiolase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,439,3330GT 8.3% 141.7 / 2.8 48V11F (GTC→TTC) pcaF‑IIbeta‑ketoadipyl‑CoA thiolase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (23/21);  new base T (2/2);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

GTATGCCGACCTGGGGCCGCTGTACCACCCGACCGCCAAGCTGCGTGAAATGGCCAAGAACGGCCAGCGCTTCTTCAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTGGCATGCACCGCAACACCCGCGCCGAAGACATGTCGGCGCACCTGATCAGCAAGCTGCTGGAACGCAACGGCAAGGTCGACCCGAAAGAAGTCGAGGACGTGATCTGGG  >  NC_002947/2439190‑2439480
                                                                                                                                               |                                                                                                                                                   
gTATGCCGACCTGGGGCCGCTGTACCACCCGACCGCCAAGCTGCGTGAAATGGCCAAGAACGGCCAGCGCTTCTTCAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAGTCCAAGAGACGTGGTGATTGTCGACt                                                                                                                                               >  1:547010/1‑150 (MQ=255)
 tATGCCGACCTGGGGCCGCTGTACCACCCGACCGCCAAGCTGCGTGAAATGGCCAAGAACGGCCAGCGCTTCTTCAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACtt                                                                                                                                              >  2:55125/1‑150 (MQ=255)
   tGCCGACCTGGGGCCGCTGTACCACCCGACCGCCAAGCTGCGTGAAATGGCCAAGAACGGCCAGCGCTTCTTCAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCg                                                                                                                                            <  1:650929/150‑1 (MQ=255)
    gCCGACCTGGGGCCGCTGTACCACCCGACCGCCAAGCTGCGTGAAATGGCCAAGAACGGCCAGCGCTTCTTCAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCgg                                                                                                                                           <  2:227608/150‑1 (MQ=255)
      cGACCTGGGGCCGATGTACCACCCGACCGCCAAGCTGCGTGAAATGGCCAAGAACGGCCAGCGCTTCTTCAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTc                                                                                                                                         <  2:1146840/150‑1 (MQ=255)
        aCCTGGGGCCGCTGTACCACCCGACCGCCAAGCTGCGTGAAATGGCCAAGAACGGCCAGCGCTTCTTCAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGc                                                                                                                                       >  2:624198/1‑150 (MQ=255)
                 cGCTGTACCACCCGACCGCCAAGCTGCGTGAAATGGCCAAGAACGGCCAGCGCTTCTTCAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATg                                                                                                                              <  1:1162631/150‑1 (MQ=255)
                 cGCTGTACCACCCGACCGCCAAGCTGCGTGAAATGGCCAAGAACGGCCAGCGCTTCTTCAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATg                                                                                                                              >  2:1222947/1‑150 (MQ=255)
                  gCTGTACCACCCGACCGCCAAGCTGCGTGAAATGGCCAAGAACGGCCAGCGCTTCTTCAACTGAGCGGTCAACGAGTTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATgg                                                                                                                             <  2:362913/150‑1 (MQ=255)
                   cTGTACCACCCGACCGCCAAGCTGCGTGAAATGGCCAAGAACGGCCAGCGCTTCTTCAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATggg                                                                                                                            >  1:410705/1‑150 (MQ=255)
                                 cGCTAAGCTGCGTGAAATGGCCAAGATCGGGCAGCGCTTCTTCAACTGAGCGGTGAATGAGATAGAGCGAGAGATTTGAGATGAGCCTGAATCCAAGAGACGTGGTGATTGTGGACTTCGGTCGCAGGCGTATGGGCCGCTTCAAGGGTg                                                                                                              <  2:94288/150‑1 (MQ=255)
                                   ccAAGCTGCGTGAAATGGCCAAGAACGGCCAGCGCTTCTTCAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAGTCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTGGc                                                                                                            <  2:547010/150‑1 (MQ=255)
                                   ccAAGCTGCGTGAAATGGCCAAGAACGGCCAGCGCTTCTTCAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTc                                                                                                                                             >  2:758319/1‑117 (MQ=255)
                                   ccAAGCTGCGTGAAATGGCCAAGAACGGCCAGCGCTTCTTCAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTc                                                                                                                                             <  1:758319/117‑1 (MQ=255)
                                    cAAGCTGCGTGAAATGGCCAAGAACGGCCAGCGCTTCTTCAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTGGCa                                                                                                           >  2:468525/1‑150 (MQ=255)
                                       gCTGCGTGAAATGGCCAAGAACGGCCAGCGCTTCTTCAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTGGCATGc                                                                                                        <  1:468525/150‑1 (MQ=255)
                                         tGCGTGAAATGGCCAAGAACGGCCAGCGCTTCTTCAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTGGCATGCAc                                                                                                      <  2:1273953/150‑1 (MQ=255)
                                          gCGTGAAATGGCCAAGAACGGCCAGCGCTTCTTCAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTg                                                                                                              <  1:1079498/141‑1 (MQ=255)
                                          gCGTGAAATGGCCAAGAACGGCCAGCGCTTCTTCAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTg                                                                                                              >  2:1079498/1‑141 (MQ=255)
                                           cGTGAAATGGCCAAGAACGGCCAGCGCTTCTTCAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATGGGCCGc                                                                                                                        <  1:1062162/130‑1 (MQ=255)
                                           cGTGAAATGGCCAAGAACGGCCAGCGCTTCTTCAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATGGGCCGc                                                                                                                        >  2:1062162/1‑130 (MQ=255)
                                                     ccAAGAACGGCCAGCGCTTCTTCAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTGGCATGCACCGCAACACCcgc                                                                                          <  1:624198/150‑1 (MQ=255)
                                                      cAAGAACGGCCAGCGCTTCTTCAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGACAATGGGCCGCTCCAAGGGTGGCATGCACCGCAACACCcgcg                                                                                         >  1:336897/1‑150 (MQ=255)
                                                             ggCCAGCGCATCTTCAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTGGCATGCACCGCAACACCCGCGCCGAAGa                                                                                  <  1:55125/150‑1 (MQ=255)
                                                              gCCAGCGCTTCTTCAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTGGCATGCACCGCAACACCCGCGCCGAAGAc                                                                                 >  2:1250600/1‑150 (MQ=255)
                                                                         ttcAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTGGCATGCACCGCAACACCCGCGCCGAAGACATGTCGGCGCa                                                                      >  1:1205188/1‑150 (MQ=255)
                                                                          tcAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTTTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTGGCATGCACCGCAACACCCGCGCCGAAGACATGTCGGCGCAc                                                                     >  1:299338/1‑150 (MQ=255)
                                                                          tcAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTTTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTGGCATGCACCGCAACACCCGCGCCGAAGACATGTCGGCGCAc                                                                     >  1:299268/1‑150 (MQ=255)
                                                                           cAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTTTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTGGCATGCACCGCAACACCCGCGCCGAAGACATGTCGGCGCAcc                                                                    <  2:299338/150‑1 (MQ=255)
                                                                           cAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTTTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTGGCATGCACCGCAACACCCGCGCCGAAGACATGTCGGCGCAcc                                                                    <  2:299268/150‑1 (MQ=255)
                                                                             aCTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATggg                                                                                                                            <  1:1261668/92‑1 (MQ=255)
                                                                             aCTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATggg                                                                                                                            >  2:1261668/1‑92 (MQ=255)
                                                                             aCTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTGGCATGCACCGCAACACCCGCGCCGAAGACATGTCGGCGCACCTg                                                                  >  1:758717/1‑150 (MQ=255)
                                                                                        aaCGCGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGCTTGACGACTTAGGTCGCACGCCAATGGGCCGCTCCAAGCGTCGCATTCACCCCAACACCCGCGCCGAAGACATGTCAGCGCACCTGCTCAACAAgct                                                       >  2:416709/1‑150 (MQ=255)
                                                                                                atagCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTGGCATGCACAGCAACACCCGCGCCGAAGACATGTCGGCGCACCTGATCAGCAAGCTGCTGGAAc                                               <  2:292649/148‑1 (MQ=255)
                                                                                                agagCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTGGCATGCACCGCAACACCCGCGCCGAAGACATGTCGGCGCACCTGATCAGCAAGCTGCTGGAAc                                               <  2:758717/150‑1 (MQ=255)
                                                                                                 gagCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTGGCATGCACCGCAACACCCGCGCCGAAGACATGTCGGCGCACCTGATCAGCAAGCTGCTGGAACg                                              >  1:748505/1‑150 (MQ=255)
                                                                                                     gagagaTTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATGAGCCGCTCCAAGGGTGGCATGCACCGCAACACCCGCGCCGAAGACATGTCGGCGCACCTGATCAGCAAGCTGCTGGAACGCAAc                                          >  2:390045/1‑150 (MQ=255)
                                                                                                      agagaTTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTGGCATGCACCGCAACACCCGCGCCGAAGACATGTCGGCGCACCTGATCAGCAAGCTGCTGGAACGCAACg                                         >  1:727879/1‑150 (MQ=255)
                                                                                                      agagaTTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTGGCATGCACCGCAACACCCGCGCCGAAGACATGTCGGCGCACCTGATCAGCAAGCTGCTGGAACGCAACg                                         <  2:410705/150‑1 (MQ=255)
                                                                                                          aTTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTGGCATGCACCGCAACACCCGCGCCGAAGACATGTCGGCGCACCTGATCAGCAAGCTGCTGGAACGCAACGGcca                                     >  1:472059/1‑148 (MQ=255)
                                                                                                              gATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTGGCATGCACCGCAACACCCGCGCCGAAGACATGTCGGCGCACCTGATCAGCAAGCTGCTGGAACGCAACGGCAAGGTc                                 >  2:941450/1‑150 (MQ=255)
                                                                                                               atatGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTGGCATGCACCGCAACACCCGCGCCGAAGACATGTCGGCGCACCTGATCAGCAAGCTGCTGGAACGCAACGGCAAGGTCg                                >  1:1189456/1‑150 (MQ=255)
                                                                                                                     gCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTGGCATGCa                                                                                                       >  2:1124814/1‑73 (MQ=255)
                                                                                                                     gCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTGGCATGCa                                                                                                       <  1:1124814/73‑1 (MQ=255)
                                                                                                                               aaGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTGGCATGCACCGCAACACCCGCGCCGAAGACATGTCGGCGCACCTGATCAGCAAGCTGCTGGAACGCAACGGCAAGGTCGACCCGAAAGAAGTCGa                >  2:835675/1‑150 (MQ=255)
                                                                                                                                     cGTGGTGATTGTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGCCGCCATGCACCGCAACGGCCGCGCCGAAGACATGTCGGCGCACCTGATCAGCAAGCTGCTGGAACGCAACGGCAAGGTCGACCCGAAAGAAGTCGAGGACGt          <  1:1250600/150‑1 (MQ=255)
                                                                                                                                             ttGTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTGGCATGCACCGCAACACCCGCGCCGAAGACATGTCGGCGCACCTGATCAGCAAGCTGCTGGAACGCAACGGCAAGGTCGACCCGAAAGAAGGCGAGGTCGTGATCTggg  <  2:1277867/150‑1 (MQ=255)
                                                                                                                                               gTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTGGCATGCACCGCAACACCcgc                                                                                          <  2:1084321/60‑1 (MQ=255)
                                                                                                                                               gTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTGGCATGCACCGCAACACCcgc                                                                                          >  1:1084321/1‑60 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
GTATGCCGACCTGGGGCCGCTGTACCACCCGACCGCCAAGCTGCGTGAAATGGCCAAGAACGGCCAGCGCTTCTTCAACTGAGCGGTCAACGAGCTAGAGCGAGAGATTTGATATGAGCCTGAATCCAAGAGACGTGGTGATTGTCGACTTCGGTCGCACGCCAATGGGCCGCTCCAAGGGTGGCATGCACCGCAACACCCGCGCCGAAGACATGTCGGCGCACCTGATCAGCAAGCTGCTGGAACGCAACGGCAAGGTCGACCCGAAAGAAGTCGAGGACGTGATCTGGG  >  NC_002947/2439190‑2439480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑