Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,481,326 G→T 8.8% R22R (CGG→AGG)  PP_2175 ← 3‑oxoacyl‑ACP reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,481,3260GT 8.8% 171.8 / 3.3 56R22R (CGG→AGG) PP_21753‑oxoacyl‑ACP reductase
Reads supporting (aligned to +/- strand):  ref base G (25/26);  new base T (3/2);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

ACCACGGCATCGACCAGGCGTTTCAGGTCCTCAACCGACTGGTTGCTACCCGTTACGCCAATACCACCAAGCTCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTACTGACATGCTGCCTCCGGAACGTTAATCGCTGAATGCTTAGGCGCACTAAAATTAGCAGCAGTCGTGGCAGCGAGGGAGACAGCTAAACTTC  >  NC_002947/2481181‑2481474
                                                                                                                                                  |                                                                                                                                                    
accacGGCATCGACCAGGCGTTTCAGGTCCTCAACCGACTGGTTGCTACCCGTTACGCCAATACCACCAAGCTCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGtgcc                                                                                                                                                  >  2:818301/1‑150 (MQ=255)
 ccacGGCATCGACCAGGCGTTTCAGGTCCTCAACCGACTGGTTGCTACCCGTTACGCCAATACCACCAAGCTCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGtgccg                                                                                                                                                 >  1:226523/1‑150 (MQ=255)
  cacGGCATCGACCAGGCGTTTCAGGTCCTCAACCGACTGGTTGCTACCCGTTACGCCAATACCACCAAGCTCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGtgccgc                                                                                                                                                <  2:703787/150‑1 (MQ=255)
      gCATCGACCAGGCGTTTCAGGTCCTCAACCGACTGGTTGCTACCCGTTACGCCAATACCACCAAGCTCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGtgccgctgcc                                                                                                                                            <  2:100711/150‑1 (MQ=255)
         tCGACCAGGCGTTTCAGGTCCTCAACCGACTGGTTGCTACCCGTTACGCCAATACCACCAAGCTCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGGCAGCGGCACGGCGCCTggccgctgacggg                                                                                                                                         <  2:515544/150‑6 (MQ=255)
            aCCAGGCGTTTCAGGTCCTCAACCGACTGGTTGCTACCCGTTACGCCAATACCACCAAGCTCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGccc                                                                                                                                      <  1:462322/150‑1 (MQ=255)
                ggCGTTTCAGGTCCTCAACCGACTGGTTGCTACCCGTTACGCCAATACCACCAAGCTCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATAc                                                                                                                                  <  2:156587/150‑1 (MQ=255)
                    tttCAGGTCCTCAACCGACTGGTTGCTACCCGTTACGCCAATACCACCAAGCTCGGCAGCCAGTGCTTCGACCTTGGCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACt                                                                                                                              <  2:944812/150‑1 (MQ=255)
                             cTCAACCGACTGGTTGCTACCCGTTACGCCAATACCACCAAGCTCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACctgc                                                                                                                     >  2:197859/1‑150 (MQ=255)
                               cAACCGACTGGTTGCTACCCGTTACGCCAATACCACCAAGCTCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACctgctg                                                                                                                   <  1:197859/150‑1 (MQ=255)
                                aaCCGACTGGTTGCTACCCGTTACGCCAATACCACAAAGCTCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑TCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCTCATa                                                                                                                                   <  2:59844/133‑1 (MQ=255)
                                aaCCGACTGGTTGCTACCCGTTACGCCAATACCACAAAGCTCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑TCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCTCATa                                                                                                                                   >  1:59844/1‑133 (MQ=255)
                                   cGACTGGTTGCTACCCGTTACGCCAATACCACCAAGCTCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGtgat                                                                                                               >  2:102363/1‑150 (MQ=255)
                                   cGACTGGTTGCTACCCGTTACGCCAATACCACCAAGCTCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGATGtgat                                                                                                               <  1:95228/150‑1 (MQ=255)
                                      cTGGTTGCTACCCGTTACGCCAATACCACCAAGCTCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTgatgat                                                                                                            <  1:818301/150‑1 (MQ=255)
                                       tGGTTGCTACCCGTTACGCCAATACCACCAAGCTCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATc                                                                                                           <  2:833831/150‑1 (MQ=255)
                                                cccGTTACGCCAATACCACCAAGCTCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCtgt                                                                                                                  >  1:123365/1‑134 (MQ=255)
                                                cccGTTACGCCAATACCACCAAGCTCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCtgt                                                                                                                  <  2:123365/134‑1 (MQ=255)
                                                cccGTTACGCCAATACCACCAAGCTCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATc                                                                                                           <  2:1250729/141‑1 (MQ=255)
                                                cccGTTACGCCAATACCACCAAGCTCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATc                                                                                                           >  1:1250729/1‑141 (MQ=255)
                                                  cGTTACGCCAATACCACCAAGCTCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTc                                                                                                >  2:546427/1‑150 (MQ=255)
                                                    ttACGCCAATACCACCAAGCTCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTa                                                                                              <  2:631660/150‑1 (MQ=255)
                                                        gCCAATACCACCAAGCTCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTACTGa                                                                                          >  2:927133/1‑150 (MQ=255)
                                                              accaccAAGCTCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTACTGACAtgct                                                                                    <  1:641275/150‑1 (MQ=255)
                                                                caccaAGCTCGGCAGCCAGTGCTTTGCCCTTGTATGAATCAAATA‑GATGACTTACCTTTAATCGTTCAGCGGCTGCGCGCCTTTTTGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTACTGACAtgctgc                                                                                  >  2:337578/1‑150 (MQ=255)
                                                                caccaAGCTCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTACTGACAtgctgc                                                                                  >  1:478830/1‑150 (MQ=255)
                                                                 accaAGCTCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTACTGACATGCTGcc                                                                                 >  1:936420/1‑150 (MQ=255)
                                                                     aGCTCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTACTGACATGCTGCCTCCg                                                                             <  1:102363/150‑1 (MQ=255)
                                                                      gCTCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTACTGACATGCTGCCTCCgg                                                                            >  1:50658/1‑150 (MQ=255)
                                                                        tCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGtgcc                                                                                                                                                  >  1:1270511/1‑78 (MQ=255)
                                                                        tCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGtgcc                                                                                                                                                  <  2:1270511/78‑1 (MQ=255)
                                                                            cAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTACTGACATGCTGCCTCCGGAACGtt                                                                      <  1:425425/150‑1 (MQ=255)
                                                                            cAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTACTGACATGCTGCCTCCGGAACGtt                                                                      <  2:226523/150‑1 (MQ=255)
                                                                              gCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCACGGCGCCTggccgctgacgg                                                                                                                                          >  1:1135334/1‑76 (MQ=25)
                                                                              gCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCACGGCGCCTggccgctgacgg                                                                                                                                          <  2:1135334/80‑2 (MQ=25)
                                                                                  gTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTACTGACATGCTGCCTCCGGAACGTTAATCGc                                                                >  2:775982/1‑150 (MQ=255)
                                                                                      ttCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTACTGACATGCTGCCTCCGGAACGTTAATCGCTGaa                                                            <  1:775982/150‑1 (MQ=255)
                                                                                                 ccGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCAGTGCCACCTGCTGTGATGATCGGAACCTTTTCTACTGACATGCTGCCTCCGGAACGTTAATCGCTGAATGCTTAGgcgc                                                 >  1:1053799/1‑150 (MQ=255)
                                                                                                 ccGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTACTGACATGCTGCCTCCGGAACGTTAATCGCTGAATGCTTAGgcgc                                                 >  2:175942/1‑150 (MQ=255)
                                                                                                 ccGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTACTGACATGCTGCCTCCGGAACGTTAATCGCTGAATGCTTAGgcgc                                                 >  2:772038/1‑150 (MQ=255)
                                                                                                         gaCAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTACTGACATGCTGCCTCCGGAACGTTAATCGCTGAATGCTTAGGCGCACTaaaa                                          >  1:255616/1‑149 (MQ=255)
                                                                                                         gaCAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTACTGACATGCTGCCTCCGGAACGTTAATCGCTGAATGCTTAGGCGCACTaaaa                                          <  2:255616/149‑1 (MQ=255)
                                                                                                            aGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGGTATGATCGCAACCTTTTCTACTGACATGCTGCCTCCGGAACGTTAATCGCTGAATGCTTAGGCGCACTAAAATTag                                      >  1:291298/1‑150 (MQ=255)
                                                                                                              gATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTACTGACATGCTGCCTCCGGAACGTTAATCGCTGAATGCTTAGGCGCACTAAAATTagca                                    <  1:546427/150‑1 (MQ=255)
                                                                                                                 g‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTACTGACATGCTGCCTCCGGAACGTTAATCGCTGAATGCTTAGGCGCACTAAAATTAgcagca                                 <  2:478830/150‑1 (MQ=255)
                                                                                                                   ccGACCTTGAACCCGTCAGCGGCCAGGCGCCGTACCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTACTGAGATGCTGCCTCCGGAACGTTAATCGCTGAATGCTTAGGCGCACTAAAATTAGcagcag                                >  1:852534/1‑150 (MQ=255)
                                                                                                                         ttGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTACTGACATGCTGCCTCCGGAACGTTAATCGCTGAATGCTTAGGCGCACTAAAATTAGCAGCAGTCGTgg                          >  1:1252909/1‑150 (MQ=255)
                                                                                                                         ttGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTACTGACATGCTGCCTCCGGAACGTTAATCGCTGAATGCTTAGGCGCACTAAAATTAGCAGCAGTCGTgg                          <  1:772038/150‑1 (MQ=255)
                                                                                                                             aCCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTACTGACATGCTGCCTcc                                                                              <  2:61816/94‑1 (MQ=255)
                                                                                                                             aCCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTACTGACATGCTGCCTcc                                                                              >  1:61816/1‑94 (MQ=255)
                                                                                                                               gcggcAGCGGCACGGCGCCTGGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTACTGACATGCTGCCTCCGGAACGTTAATCGCTGAATGCTTAGGCGCACTAAAATTAGCAGCAGTCGTGGCAGCGa                    >  2:531092/5‑150 (MQ=255)
                                                                                                                                  tCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTACTGACATGCTGCCTCCGGAACGTTAATCGCTGAATGCTTAGGCGCACTAAAATTAGCAGCAGTCGTGGCAGCGAggg                 <  2:936420/150‑1 (MQ=255)
                                                                                                                                      cGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTACTGACATGCTGCCTCCGGAACGTTAATCGCTGAATGCTTAGGCGCACTAAAATTAGCAGCAGTCGTGGCAGCGAGGGAGAc             >  1:621471/1‑150 (MQ=255)
                                                                                                                                        gCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTACTGACATGCTGCCTCCGGAACGTTAATCGCTGAATGCTTAGGCGCACTAAAATTAGCAGCAGTCGTGGCAGCGAGGGAGACAg           >  1:1118186/1‑150 (MQ=255)
                                                                                                                                                 cGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTACTGACATGCTGCCTCCGGAACGTTAATCGCTGAATGCTTAGGCGCACTAAAATTAGCAGCAGTCGTGGCAGCGAGGGAGACAGCTAAACTTc  >  2:657215/1‑150 (MQ=255)
                                                                                                                                                 cGTGCCGCTGCCGCGCCCATACCACTGCCAACTGCTGTGACGATCACCCTGCTGCCTTTGGTCATGCTGCCTCCGGCACGTTAATCGCTGAATGCTTAGGCGCACTAAAATTAGCAGCAGTCGTGGCAGCGAGGGAGACAGCTAAACTTc  <  1:927133/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
ACCACGGCATCGACCAGGCGTTTCAGGTCCTCAACCGACTGGTTGCTACCCGTTACGCCAATACCACCAAGCTCGGCAGCCAGTGCTTCGCCCTTGCCCGAGGAGGACAGGATG‑CCGACCTTGAACCCGTCAGCGGCCAGGCGCCGTGCCGCTGCCGCGCCCATACCACTGCCACCTGCTGTGATGATCGCAACCTTTTCTACTGACATGCTGCCTCCGGAACGTTAATCGCTGAATGCTTAGGCGCACTAAAATTAGCAGCAGTCGTGGCAGCGAGGGAGACAGCTAAACTTC  >  NC_002947/2481181‑2481474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑