Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,586,840 C→T 8.7% Y54Y (TAC→TAT)  PP_2266 → DNA‑directed RNA polymerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,586,8400CT 8.7% 122.9 / 3.2 46Y54Y (TAC→TAT) PP_2266DNA‑directed RNA polymerase
Reads supporting (aligned to +/- strand):  ref base C (18/24);  new base T (2/2);  total (20/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.51e-01

TCCTCTAGTTGGCCGCCTGGATGGTGAAGACCTCATCGCCACCCAAGTCCGTCTGGAAGAGGAGATGACCCAGCGCGGTGTGAGCCGTTTCCAGCGCAACGTAGAGGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCATCAAGGCGTGGATGGATGAGTCTGAGGCCGGTGTGTCGTCCCGCTGGGGCAGCGCATACAAGCTCCTCCGTGGCTACGATGCGGATGTCCTGGCCTAC  >  NC_002947/2586696‑2586984
                                                                                                                                                |                                                                                                                                                
tcctcTAGTTGGCCGCCTGGATGGTGAAGACCTCATCGCCACCCAAGTCCGTCTGGAAGAGGAGATGACCCAGCGCGGTGTGAGCCGTTTCCAGCGCAACGTAGAGATCGACTGGAGGATGGGCGCTGAGGATGGCACCAAGTACggatg                                                                                                                                             >  1:12469/1‑147 (MQ=255)
   tctAGTTGGCCGCCTGGATGGTGAAGACCTCATCGCCACCCAAGTCCGTCTGGAAGAGGAGATGACCCAGCGCGGTGTGAGCCGTTTCCAGCGCAACGTAGAGGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCcat                                                                                                                                          >  1:937601/1‑150 (MQ=255)
           gccgccTGGATGGTGAAGACCTCATCGCCACCCAAGTCCGTCTGGAAGAGGAGATGACCCAGCGCGGTGTGAGCCGTTTCCAGCGCAACGTAGAGGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCCATCATCGtct                                                                                                                                  >  2:575494/1‑150 (MQ=255)
           gccgccTGGATGGTGAAGACCTCATCGCCACCCAAGTCCGTCTGGAAGAGGAGATGACCCAGCGCGGTGTGAGCCGTTTCCAGCGCAACGTAGAGGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCCATCATCGtct                                                                                                                                  >  2:575114/1‑150 (MQ=255)
            ccgccTGGATGGTGAAGACCTCATCGCCACCCAAGTCCGTCTGGAAGAGGAGATGACCCAGCGCGGTGTGAGCCGTTTCCAGCGCAACGTAGAGGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCCATCATCGtctc                                                                                                                                 <  2:854827/150‑1 (MQ=255)
               ccTGGATGGTGAAGACCTCATCGCCACCCAAGTCCGTCTGGAAGAGGAGATGACCCAGCGCGGGGTGAGCCGGTTCCAGCGCAACGTAGAGGCCGCCCGGAGGATGGGCGGTGAGGATGGCACCGAGTACGGCTCCATCATCGTGTCCga                                                                                                                              >  1:504449/1‑149 (MQ=255)
                         gAAGACCTCATCGCCACCCAAGTCCGTCTGGAAGAGGAGATGACCCAGCGCGGTGTGAGCCGTTTCCAGCGCAACGTAGAGGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTATGGCTCCATCATCGTCTCCGGCCGCATGAAg                                                                                                                    >  1:144805/1‑150 (MQ=255)
                         gAAGACCTCATCGCCACCCAAGTCCGTCTGGAAGAGGAGATGACCCAGCGCGGTGTGAGCCGTTTCCAGCGCAACGTAGAGGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAg                                                                                                                    <  1:575494/150‑1 (MQ=255)
                         gAAGACCTCATCGCCAACCAAGTCCGTCTGGAAGAGGAGATGACCCAGCGCGGTGTGAGCCGTTTCCAGCGCAACGTAGAGGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAg                                                                                                                    <  1:575114/150‑1 (MQ=255)
                                  aTCGCCACCCAAGTCCGTCTGGAAGAGGAGATGACCCAGCGCGGTGTGAGCCGTTTCCAGCGCAACGTAGAGGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTATGGCTCCATCATCGTCTCCg                                                                                                                               <  1:215474/130‑1 (MQ=255)
                                  aTCGCCACCCAAGTCCGTCTGGAAGAGGAGATGACCCAGCGCGGTGTGAGCCGTTTCCAGCGCAACGTAGAGGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTATGGCTCCATCATCGTCTCCg                                                                                                                               >  2:215474/1‑130 (MQ=255)
                                    cGCCACCCAAGTCCGTCTGGAAGAGGAGATGACCCAGCGCGGTGTGAGCCGTTTCCAGCGCAACGTAGAGGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGa                                                                                                         <  2:80428/150‑1 (MQ=255)
                                       cACCCAAGTCCGTCTGGAAGAGGAGATGACCCAGCGCGGTGTGAGCCGTTTCCAGCGCAACGTAGAGGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGc                                                                                                      <  1:302467/150‑1 (MQ=255)
                                          ccAAGTCCGTCTGGAAGAGGAGATGACCCAGCGCGGTGTGAGCCGTTTCCAGCGCAACGTAGAGGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCAt                                                                                                   >  1:174170/1‑150 (MQ=255)
                                              gTCCGTCTGGAAGAGGAGATGACCCAGCGCGGTGTGAGCCGTTTCCAGCGCAACGTAGAGGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCATCAAg                                                                                               <  2:1257794/150‑1 (MQ=255)
                                                      ggAAGAGGAGATGACCCAGCGCGGTGTGCGCCGTTTCCAGCGCAACGTAGAGGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCATCAAGGCGtggat                                                                                       <  1:943863/150‑1 (MQ=255)
                                                         agagGAGATGACCCAGCGCGGTGTGAGCCGTTTCCAGCGCAACGTAGAGGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCATCAAGGCGtggatgga                                                                                    >  2:122175/1‑150 (MQ=255)
                                                               gaTGACCCAGCGCGGTGTGAGCCGTTTCCAGCGCAACGTAGAGGCCGCCCGGAGGCTGCGGGCTGAGGATGGCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCATCAAGGCGTGGATGGATGAGTc                                                                              >  2:175032/1‑150 (MQ=255)
                                                                  gACCCAGCGCGGTGTGAGCCGTTTCCAGCGCAACGTAGAGGCCGCCCGGAGGATGGGCGCTTAGGATGGCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCATCAAGGCGTAGATGGATGAGTCTGa                                                                           <  2:174170/150‑1 (MQ=255)
                                                                     ccAGCGCGGTGTGAGCCGTTTCCAGCGCAACGTAGAGGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCATCAAGGCGTGGATGGATGAGTCTGAGGc                                                                        >  1:470953/1‑150 (MQ=255)
                                                                     ccAGCGCGGTGTGAGCCGTTTCCAGCGCAACGTAGAGGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCATCAAGGCGTGGATGGATGAGTCTGAGGc                                                                        >  2:1196372/1‑150 (MQ=255)
                                                                          gcgGTGTGAGCCGTTTCCAGCGCAACGTAGAGGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCATCAAGGCGTGGATGGATGAGTCTGAGGCCGgtg                                                                   <  2:937601/150‑1 (MQ=255)
                                                                            ggTGTGAGCCGTTTCCAGCGCAACGTAGAGGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCATCAAGGCGTGGATGGATGAGTCTGAGGCCGgtgtg                                                                 <  2:926144/150‑1 (MQ=255)
                                                                                tgAGCCGTTTCCAGCGCAACGTAGAGGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCCAGCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCCTCCTGTCGTGGATGGATGAGTCTGAGGCCGGTGTgtcgt                                                             <  1:1066430/150‑1 (MQ=255)
                                                                                     cGTTTCCAGCGCAACGTAGAGGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCATCAAGGCGTGGATGGATGAGTCTGAGGCCGGTGTGTCGTCCCGc                                                        >  2:376887/1‑150 (MQ=255)
                                                                                         tCCAGCGCAACGTAGAGGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCCATCATGGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCATCAAGGCGTGGATGGATGAGTCTGAGGCCGGTGTGTCGTCCCGCTggg                                                    <  1:1185016/150‑1 (MQ=255)
                                                                                                cAACGTAGAGGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCATCAAGGCGTGGATGGATGAGTCTGAGGCCGGTGTGTCGTCCCGCTGGGGCAgcgc                                             <  1:889037/150‑1 (MQ=255)
                                                                                                cAACGTAGAGGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCATCAAGGCGTGGATGGATGAGTCTGAGGCCGGTGTGTCGTCCCGCTGGGGCAgcgc                                             >  1:1272957/1‑150 (MQ=255)
                                                                                                 aaCGTAGAGGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCATCAAGGCGTGGATGGATGAGTCTGAGGCCGGTGTGTCGTCCCGCTGGGGCAGCGCa                                            <  1:376887/150‑1 (MQ=255)
                                                                                                  tcGTAGAGGCCGCCCGGAGGATGGTCGCTGAGGATGGCACCGAGTACTGCTCCATCATCGTCTCCGGCCGTATGAAGGCGCTCGCTGAGGCCATCAAGGCGTGGATGGATGAGTCTGAGTCCGGTGTGTCGTTCCTCTGGGGCAGCTCAt                                           <  2:265793/149‑1 (MQ=255)
                                                                                                    gTAGAGGCCGCCCGGAGGGTGGGCGCTGAGGATGGCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGTGGCCATCAAGGCGTGGATGGATGAGTCTGAGGCCGGTGTGTCGTCCCGCTGGGGCAGCGCATAc                                         >  1:937788/1‑150 (MQ=255)
                                                                                                     tAGAGGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCATCAAGGCGTGGATGGATGAGTCTGAGGCCGGTGTgtcgt                                                             <  2:664993/129‑1 (MQ=255)
                                                                                                     tAGAGGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGATCGCTGAGGCCATCAAGGCGTGGATGGATGAGTCTGAGGCCGGTGTgtcgt                                                             >  1:664993/1‑129 (MQ=255)
                                                                                                        agGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCATCAAGGCGTGGATGGATGAGTCTGAGGCCGGTGTGTCGTCCCGCTGGGGCAGCGCATACAAGc                                     <  2:442143/150‑1 (MQ=255)
                                                                                                        agGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCATCAAGGCGTGGATGGATGAGTCTGAGGCCGGTGTGTCGTCCCGCTGGGGCAGCGCATACAAGc                                     <  2:442237/150‑1 (MQ=255)
                                                                                                          gccgccCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCATCAAGGCGTGGATGGATGAGTCTGa                                                                           <  1:519834/110‑1 (MQ=255)
                                                                                                          gccgccCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCATCAAGGCGTGGATGGATGAGTCTGa                                                                           >  2:519834/1‑110 (MQ=255)
                                                                                                              cccGGAGGATGGGCGCTGAGGATGGACCCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGTGGCCATCAAGGCGTGGATGGATGAGTCTGAGGCCGGTGTGTCGTCCCGCTGGGGCAGCGCATACAAGCtcctcc                               <  2:937788/150‑1 (MQ=255)
                                                                                                                              tGAGGATGGCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCATCAAGGCGTGGATGGATGAGTCTGAGGCCGGTGTGTCGTCCCGCTGGGGCAGCGCATACAAGctcctc                                <  2:104235/133‑1 (MQ=255)
                                                                                                                              tGAGGATGGCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCATCAAGGCGTGGATGGATGAGTCTGAGGCCGGTGTGTCGTCCCGCTGGGGCAGCGCATACAAGctcctc                                >  1:104235/1‑133 (MQ=255)
                                                                                                                                     ggCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCATCAAGGCGTGGATGGATGAGTCTGAGGCCGGTGTGTCGTCCCGCTGGGGCAGCGCATACAAGCTCCTCCGTGGCTACGATGCGGATGTCCTg        <  1:175032/150‑1 (MQ=255)
                                                                                                                                      gCACCGAGTATGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTAGCTGAGGCCATCAAGGCGTGGATGGATGAGTCTGAGGCCGGTGTTTCTTCCCGCTTGGGCAGCGCATACAAGCTCCTCCGTGGCTACGATGCGGATGTCCTgg       <  2:144805/150‑1 (MQ=255)
                                                                                                                                      gCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCATCAAGGCGTGGATGGATGAGTCTGAGGCCGGTGTGTCGTCCCGCTGGGGCAGCGCATACAAg                                      >  2:180783/1‑119 (MQ=255)
                                                                                                                                      gCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCATCAAGGCGTGGATGGATGAGTCTGAGGCCGGTGTGTCGTCCCGCTGGGGCAGCGCATACAAg                                      <  1:180783/119‑1 (MQ=255)
                                                                                                                                        aCCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCATCAAGGCGTGGATGGATGAGTCTGAGGCCGGTGTGTCGTCCCGCTGGGGCAGCGCATACAAGCTCCTCCGTTGCTACGATGCGGATGTCCTGGcc     >  2:244913/1‑150 (MQ=255)
                                                                                                                                           gAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCATCAAGGCGTGGATGGATGAGTCTGAGGCCGGTGTGTCGTCCCGCTGGGGCAGCGCATACAAGCTCCTCCGTGGCTACGATGCGGATGTCCTGGCCTAc  <  1:122175/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TCCTCTAGTTGGCCGCCTGGATGGTGAAGACCTCATCGCCACCCAAGTCCGTCTGGAAGAGGAGATGACCCAGCGCGGTGTGAGCCGTTTCCAGCGCAACGTAGAGGCCGCCCGGAGGATGGGCGCTGAGGATGGCACCGAGTACGGCTCCATCATCGTCTCCGGCCGCATGAAGGCGCTCGCTGAGGCCATCAAGGCGTGGATGGATGAGTCTGAGGCCGGTGTGTCGTCCCGCTGGGGCAGCGCATACAAGCTCCTCCGTGGCTACGATGCGGATGTCCTGGCCTAC  >  NC_002947/2586696‑2586984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑