Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,610,874 G→T 7.8% E658* (GAG→TAG)  PP_2286 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,610,8740GT 7.8% 155.4 / 2.4 51E658* (GAG→TAG) PP_2286hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (24/23);  new base T (2/2);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

GAACATCAACAACACCCTGTACCTGAATAAAGACCTGCCTCAAGTCCCCAAGGGAGAAGACCGGGGGAAGTGGGTAGAGCGCTTCATTGACGAGGTGCCAGGGAAGATCGCGGATGACCAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTCACAGACGTGAATCACCAAGTCCTCAACTACTCCCGCCAACAGGTGGAGAAGTGGATCGGCAACACCTACGCCGCAGACCTCAAGACCAAGACAGCCG  >  NC_002947/2610726‑2611021
                                                                                                                                                    |                                                                                                                                                   
gAACATCAACAACACCCTGTACCTGAATAAAGACCTGCCTCAAGTCCCCAAGGGAGAAGACCGGGGGAAGTGGGTAGAGCGCTTCATTGACGAGGTGCCAGGGAAGATCGCGGATGACCAAGACATCGGCAAGGGGAATATCCGCATGGa                                                                                                                                                    >  2:384385/1‑150 (MQ=255)
    aTCAACAACACCCTGTACCTGAATAAAGACCTGCCTCAAGTCCCCAAGGGAGAAGACCGGGGGAAGTGGGTAGAGCGCTTCATTGACGAGGTGCCAGGGAAGATCGCGGATGACCAAGACATCGGCAAGGGGAATATCCGCATGGAGccc                                                                                                                                                >  2:1143069/1‑150 (MQ=255)
                     ccTGAATAAAGACCTGCCTCAAGTCCCCAAGGGAGAAGACCGGGGGAAGTGGGTAGAGCGCTTCATTGACGAGGTGCCAGGGAAGATCGCGGATGACCAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTa                                                                                                                               <  1:384385/150‑1 (MQ=255)
                      ctcaaTAAAGACCTGCCCCAAGTCCCCAACGGAGAAGACCGGGGGAAGTGGGTAGAGCGCATCATTGACGACGTGCCAGGCAAGTTCGCTGATGACCAAGACATCGGCAAGGTTTATATCCGCATGGAGCCCAATGACCGAGGCTGTTac                                                                                                                              <  2:435888/147‑1 (MQ=255)
                        gAATAAAGACCTGCCTCAAGTCCCCAAGGGAGAAGACCGGGGGAAGTGGGTAGAGCGCTTCATTGACGAGGTGCCAGGGAAGATCGCGGATGACCAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATTACCGAGGCGGTTacac                                                                                                                            >  1:1212182/1‑150 (MQ=255)
                                aCCTGCCTCAAGTCCCCAAGGGAGAAGACCGGGGGAAGTGGGTAGAGCGCTTCATTGACGAGGTGCCAGGGAAGATCGCGGATGACCAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCAtgt                                                                                                                       >  1:360968/1‑147 (MQ=255)
                                aCCTGCCTCAAGTCCCCAAGGGAGAAGACCGGGGGAAGTGGGTAGAGCGCTTCATTGACGAGGTGCCAGGGAAGATCGCGGATGACCAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCAtgt                                                                                                                       <  2:360968/147‑1 (MQ=255)
                                   tGCCTCAAGTCCCCAAGGGAGAAGACCGGGGGAAGTGGGTAGAGCGCTTCATTGACGAGGTGCCAGGGAAGATCGCGGATGACCAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCaa                                                                                                                                              <  2:253860/121‑1 (MQ=255)
                                   tGCCTCAAGTCCCCAAGGGAGAAGACCGGGGGAAGTGGGTAGAGCGCTTCATTGACGAGGTGCCAGGGAAGATCGCGGATGACCAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCaa                                                                                                                                              >  1:253860/1‑121 (MQ=255)
                                         aaGTCCCCAAGGGAGAAGACCGGGGGAAGTGGGTAGAGCGCTTCATTGACGAGGTGCCAGGGAAGATCGCGGATGACCAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCg                                                                                                                                   <  1:1034674/126‑1 (MQ=255)
                                         aaGTCCCCAAGGGAGAAGACCGGGGGAAGTGGGTAGAGCGCTTCATTGACGAGGTGCCAGGGAAGATCGCGGATGACCAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCg                                                                                                                                   >  2:1034674/1‑126 (MQ=255)
                                           gTCCCCAAGGGAGAAGACCGGGGGAAGTGGGTAGAGCGCTTCATTGACGAGGTGCCAGGGAAGATCGCGGATGACCAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTg                                                                                                         >  1:372367/1‑150 (MQ=255)
                                              cccAAGGGAGAAGACCGGGGGAAGTGGGTAGAGCGCTTCATTGACGAGGTGCCAGGGAAGATCGCGGATGACCAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCAt                                                                                                                         <  1:1222086/131‑1 (MQ=255)
                                              cccAAGGGAGAAGACCGGGGGAAGTGGGTAGAGCGCTTCATTGACGAGGTGCCAGGGAAGATCGCGGATGACCAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCAt                                                                                                                         >  2:1222086/1‑131 (MQ=255)
                                                cAAGGGAGAAGACCGGGGGAAGTGGGTAGAGCGCTTCATTGACGAGGTGCCAGGGAAGATCGCGGATGACCAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGt                                                                                                                                 <  1:1116051/121‑1 (MQ=255)
                                                cAAGGGAGAAGACCGGGGGAAGTGGGTAGAGCGCTTCATTGACGAGGTGCCAGGGAAGATCGCGGATGACCAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGt                                                                                                                                 >  2:1116051/1‑121 (MQ=255)
                                                     gagaAGACCGGGGGAAGTGGGTAGAGCGCTTCATTGACGAGGTGCCAGGGAAGATCGCGGATGACCAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTCACAg                                                                                               <  1:1143069/150‑1 (MQ=255)
                                                       gaagaCCGGGGGAAGTGGGTAGAGCGCTTCATTGACGAGGTGCCAGGGAAGATCGCGGATGACCAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTca                                                                                                  <  1:1195195/145‑1 (MQ=255)
                                                       gaagaCCGGGGGAAGTGGGTAGAGCGCTTCATTGACGAGGTGCCAGGGAAGATCGCGGATGACCAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTca                                                                                                  >  2:1195195/1‑145 (MQ=255)
                                                                   aaGTGGGTAGAGCGCTTCATTGACGAGGTGCCAGGGAAGATCGCGGATGACCAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTCCCAGACGTGAATCACCaa                                                                                 <  2:669855/150‑1 (MQ=255)
                                                                   aaGTGGGTAGAGCGCTTCATTGACGAGGTGCCAGGGAAGATCGCGGATGACCAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTCACAGACGTGAATCACCaa                                                                                 <  2:372367/150‑1 (MQ=255)
                                                                             agCGCTTCATTGACGAGGTGCCAGGGAAGATCGCGGATGACCAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTg                                                                                                         <  1:1135447/116‑1 (MQ=255)
                                                                             agCGCTTCATTGACGAGGTGCCAGGGAAGATCGCGGATGACCAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTg                                                                                                         >  2:1135447/1‑116 (MQ=255)
                                                                                           gAGGTGCCAGGGAAGATCGCGGATGACCAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTca                                                                                                  >  2:897931/1‑109 (MQ=255)
                                                                                           gAGGTGCCAGGGAAGATCGCGGATGACCAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTca                                                                                                  <  1:897931/109‑1 (MQ=255)
                                                                                                   aGGGAAGATCGCGGATGACCAAGACATCGGCAAGGGGAATATCCGCATGTAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTCACAGACGTGa                                                                                         >  1:137238/1‑110 (MQ=255)
                                                                                                   aGGGAAGATCGCGGATGACCAAGACATCGGCAAGGGGAATATCCGCATGTAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTCACAGACGTGa                                                                                         <  2:137238/110‑1 (MQ=255)
                                                                                                     ggAAGATCGCGGATGACCAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTCACAGACGTGAATCACCAAGTCCTCAACTACTCCCGCCAACAGGTGGAGAAGt                                               >  1:1029156/1‑150 (MQ=255)
                                                                                                               ggATGACCAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTCACAGACGTGAATCACCAAGTCCTCAACTACTCCCGCCAACAGGTGGAGAAGTGGATCGGCaa                                     <  1:4368/150‑1 (MQ=255)
                                                                                                                   gACCAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAATGCCCCTCACAGACGTGAATCACCAAGTCCTCAACTACTCCCGCCAACAGGTGGAGAAGTGGATCGGCAACAcc                                 >  1:248979/1‑150 (MQ=255)
                                                                                                                     ccAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTCACAGACGTGAATCACCAAGTCCTCAACTACTCCCGCCAACAGGTg                                                      >  1:767145/1‑127 (MQ=255)
                                                                                                                     ccAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTCACAGACGTGAATCACCAAGTCCTCAACTACTCCCGCCAACAGGTg                                                      <  2:767145/127‑1 (MQ=255)
                                                                                                                      cAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTCACAGACGTGAATCACCAAGTCCTCAactact                                                                    <  2:58818/112‑1 (MQ=255)
                                                                                                                      cAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTCACAGACGTGAATCACCAAGTCCTCAACTACTCCCGCCAACAGGTGGAGAAGTGGATCGGCAACACCTAc                              >  1:1080965/1‑150 (MQ=255)
                                                                                                                      cAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTCACAGACGTGAATCACAAAGTCGTCAactact                                                                    >  1:58818/1‑112 (MQ=255)
                                                                                                                           cATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTCACAGACGTGAATCACCAAGTCCTCAACTACTCCCGCCAACAGGt                                                       >  1:1238996/1‑120 (MQ=255)
                                                                                                                           cATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTCACAGACGTGAATCACCAAGTCCTCAACTACTCCCGCCAACAGGt                                                       <  2:1238996/120‑1 (MQ=255)
                                                                                                                            aTCGGCAAGGGGAATATCTGCATGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTCACAGACGTGAATCACCAAGTCCTCAACTACTCCCGCCAACAGGTGGAGAAGTGGATCGGCAACACCTACGCCGCa                        <  1:283710/150‑1 (MQ=255)
                                                                                                                            aTCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTCACAGACGTGAATCACCAAGTCCTCAACTACTCCCGCCAACAGGTGGGGAAGTGTATCTGCAACACCTACgcagca                        >  2:209784/1‑150 (MQ=255)
                                                                                                                            aTCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTCACAGACGTGAATCACCAAGTCCTCAACTACTCCCGCCAACAGGTGGAGAAGTGGATCGGCAacac                                  <  2:605238/140‑1 (MQ=255)
                                                                                                                            aTCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTCACAGACGTGAATCACCAAGTCCTCAACTACTCCCGCCAACAGGTGGAGAAGTGGATCGGCAacac                                  >  1:605238/1‑140 (MQ=255)
                                                                                                                            aTCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTCACAGACGTGAATCACCAAGTCCTCAACTACTCCCGCCAACAGGTGGAGAAGTGGATCGGCAACACCTACGCCGCa                        >  1:520539/1‑150 (MQ=255)
                                                                                                                                gCAAGGGGAATATCCGCATGTAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTCACAGACGTGAATCACCAAGTCCTCAACTACTCCCGCCAACAGGTGGAGAAGTgg                                             >  2:490379/1‑125 (MQ=255)
                                                                                                                                gCAAGGGGAATATCCGCATGTAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTCACAGACGTGAATCACCAAGTCCTCAACTACTCCCGCCAACAGGTGGAGAAGTgg                                             <  1:490379/125‑1 (MQ=255)
                                                                                                                                gCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTCACAGACGTGAATCACCAAGTCCTCAACTACTCCCGCCAACAGGTGGAGAAGTGGATCGGCAaca                                   >  1:446336/1‑135 (MQ=255)
                                                                                                                                gCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTCACAGACGTGAATCACCAAGTCCTCAACTACTCCCGCCAACAGGTGGAGAAGTGGATCGGCAaca                                   <  2:446336/135‑1 (MQ=255)
                                                                                                                                          tatCCGCATGGAGCCCAATTACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTCACAGACGTGAATCACCAAGTCCTCAACTACTCCCGCCAACAGGTGGAGAAGTGGATCGGCAACACCTACGCCGCAGACCTcaagaccaa          <  2:1212182/150‑1 (MQ=255)
                                                                                                                                               gCATGGAGCCCAATGACCGAGGCGGTTACACGATGTGGACCGGGGGAGTGCCCCTCACAGACGTGAATCACCAAGTCCTCAACTACTCCCGCCAACAGGTGGAGAAGTGGATCGGCAACACCTACGCCGCAGACCTCAAGACCAAGACAg     >  2:788498/1‑150 (MQ=255)
                                                                                                                                                 aTGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTCACAGACGTGAATCACCAAGTCCTCATCTACTCCCGCCAACAGGt                                                       >  1:887870/1‑98 (MQ=255)
                                                                                                                                                 aTGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTCACAGACGTGAATCACCAAGTCCTCATCTACTCCCGCCAACAGGt                                                       <  2:887870/98‑1 (MQ=255)
                                                                                                                                                  tGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTCACAGACGTGAATCACCAAGTCCTCAACTACTCCCGCCAACAGGTGGAGAAGTGGATCGGCAACACCTACGCCGCAGACCTCAAGACCAAGACAGCCg  <  1:835854/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAACATCAACAACACCCTGTACCTGAATAAAGACCTGCCTCAAGTCCCCAAGGGAGAAGACCGGGGGAAGTGGGTAGAGCGCTTCATTGACGAGGTGCCAGGGAAGATCGCGGATGACCAAGACATCGGCAAGGGGAATATCCGCATGGAGCCCAATGACCGAGGCGGTTACACCATGTGGACCGGGGGAGTGCCCCTCACAGACGTGAATCACCAAGTCCTCAACTACTCCCGCCAACAGGTGGAGAAGTGGATCGGCAACACCTACGCCGCAGACCTCAAGACCAAGACAGCCG  >  NC_002947/2610726‑2611021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑