Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,631,188 C→G 9.6% Q204E (CAG→GAG)  lon‑II → DNA‑binding ATP‑dependent protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,631,1880CG 9.6% 115.1 / 3.4 41Q204E (CAG→GAG) lon‑IIDNA‑binding ATP‑dependent protease
Reads supporting (aligned to +/- strand):  ref base C (18/19);  new base G (2/2);  total (20/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.86e-01

TCGACGAGCCAGGGCGCCTGGTCGACACCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGCCACAATGAAGTCGAAGAGCTGAAAAAGCGC  >  NC_002947/2631042‑2631337
                                                                                                                                                  |                                                                                                                                                     
tCGACGAGCCAGGGCGCCTGGTCGACACCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAgg                                                                                                                                                    >  2:961917/1‑150 (MQ=255)
  gacgaGCCAGGGCGCCTGATCGACACCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAACTCGTCGACTTGACTACCCGTATCGAGCACGTGCTGGCGCTGCTTGATGACGAAATCGACGTCCTGCAgggc                                                                                                                                                  >  2:106617/1‑148 (MQ=255)
       gCCAGGGCGCCTGGTCGACACCATGGCCGCGCACATGGCCCTGAAGCTCCAATGGAAGCAGGAAATTCTTGAAATCGTCTACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGaaaa                                                                                                                                             <  2:998962/150‑1 (MQ=255)
        ccAGGGCGCCTGGTCGACACCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTGCAGGAGGTCGatttc                                                                                                                                            <  1:1124931/150‑5 (MQ=255)
        ccAGGGCGCCTGGTCGACACCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAg                                                                                                                                            >  1:92076/1‑150 (MQ=255)
          aGGGCGCCTGGTCGACACCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTGCAGGAGGTCGat                                                                                                                                               >  1:166390/1‑144 (MQ=255)
          aGGGCGCCTGGTCGACACCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTGCAGGAGGTCGat                                                                                                                                               <  2:166390/145‑2 (MQ=255)
            ggCGCCTGGTCGACACCATGGCCGCGCACATGGCACTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCAACCTCCTGCAGGTCGAAAAGCGCa                                                                                                                                        <  2:438474/150‑1 (MQ=255)
               gcCTGGTCGACACCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGCGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTc                                                                                                                                     >  1:1247196/1‑150 (MQ=255)
                       gACACCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCgg                                                                                                                             >  1:124101/1‑150 (MQ=255)
                         cacCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGt                                                                                                                           <  1:440216/150‑1 (MQ=255)
                            cATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGTATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCaa                                                                                                                        >  2:1076474/1‑150 (MQ=255)
                             aTGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCaag                                                                                                                       >  1:1076913/1‑150 (MQ=255)
                                             cccTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGTATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGca                                                                                                       <  1:1076474/150‑1 (MQ=255)
                                                tGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCg                                                                                                                              >  2:791679/1‑124 (MQ=255)
                                                tGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCg                                                                                                                              <  1:791679/124‑1 (MQ=255)
                                                        gAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCgg                                                                                                                             >  2:451015/1‑117 (MQ=255)
                                                        gAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCgg                                                                                                                             <  1:451015/117‑1 (MQ=255)
                                                             gAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGtacta                                                                                       >  2:28507/1‑150 (MQ=255)
                                                              aaGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGtactac                                                                                      <  2:1247196/150‑1 (MQ=255)
                                                                gCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCt                                                                                    >  1:764463/1‑150 (MQ=255)
                                                                   ggAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGaa                                                                                 >  1:646768/1‑150 (MQ=255)
                                                                   ggAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGaa                                                                                 <  2:646768/150‑1 (MQ=255)
                                                                    gAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAAt                                                                                >  2:799553/1‑150 (MQ=255)
                                                                    gAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAAt                                                                                <  2:764463/150‑1 (MQ=255)
                                                                                aTCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAg                                                                    <  2:92076/150‑1 (MQ=255)
                                                                                        cTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAATGCCATTCa                                                            <  1:629481/150‑1 (MQ=255)
                                                                                           gACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGCCAACCCCGTGATCAAAAAGCGGACGCGCATGGGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCagaa                                                         >  1:1121082/1‑150 (MQ=255)
                                                                                                  cGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACtt                                                  >  2:542911/1‑150 (MQ=255)
                                                                                                        gAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTGCCTGAATGAGCAGATGAAgg                                                                   <  2:238773/127‑1 (MQ=255)
                                                                                                        gAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTGCCTGAATGAGCAGATGAAgg                                                                   >  1:238773/1‑127 (MQ=255)
                                                                                                          gCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATgg                                          <  2:124101/150‑1 (MQ=255)
                                                                                                           cACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGt                                         <  1:257105/150‑1 (MQ=255)
                                                                                                              gTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGta                                                                                          <  2:806638/98‑1 (MQ=255)
                                                                                                              gTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGta                                                                                          >  1:806638/1‑98 (MQ=255)
                                                                                                                          ctgGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCACGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGCCACAAt                          >  1:356491/1‑150 (MQ=255)
                                                                                                                              aTGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCCAGAAAACTACCTGAATGAGCAGATCAGGGCCAGTCACAAAGGACTTGGCGATGGTGACGAAGGCCACAATGAAg                      <  1:988969/150‑1 (MQ=255)
                                                                                                                                gCCGAAAGCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGCCACAATGAAGTc                    <  1:961917/150‑1 (MQ=255)
                                                                                                                                         gACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGCCACAATGaa                       <  1:1193674/138‑1 (MQ=255)
                                                                                                                                         gACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGCCACAATGaa                       >  2:1193674/1‑138 (MQ=255)
                                                                                                                                                tGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGCCACAATGAAGTCGAAGAGCTGAAAAAgc    <  1:799553/150‑1 (MQ=255)
                                                                                                                                                  cAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGCCACAATGAAGTCGAAGAGCTGAAACAgcgc  >  1:342703/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
TCGACGAGCCAGGGCGCCTGGTCGACACCATGGCCGCGCACATGGCCCTGAAGATCGAGCAGAAGCAGGAAATTCTTGAAATCGTCGACTTGACTACCCGTGTCGAGCACGTGCTGGCGCTGCTGGATGCCGAAATCGACCTCCTGCAGGTCGAAAAGCGCATTCGCGGCCGGGTCAAGAAGCAGATGGAGCGCAGCCAGCGCGAGTACTACCTGAATGAGCAGATGAAGGCCATTCAGAAAGAACTTGGCGATGGTGACGAAGGCCACAATGAAGTCGAAGAGCTGAAAAAGCGC  >  NC_002947/2631042‑2631337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑