Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,799,229 C→T 9.3% intergenic (‑200/+16) PP_2452 ← / ← ansB hypothetical protein/glutaminase‑asparaginase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,799,2290CT 9.3% 206.1 / 9.2 75intergenic (‑200/+16)PP_2452/ansBhypothetical protein/glutaminase‑asparaginase
Reads supporting (aligned to +/- strand):  ref base C (34/34);  new base T (4/3);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

AAATTCGCAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGT  >  NC_002947/2799080‑2799377
                                                                                                                                                     |                                                                                                                                                    
aaaTTCGCAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGcc                                                                                                                                                      <  2:1208661/150‑1 (MQ=255)
     cGCAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGGGCCGGTGCGCCGGCTCTGAg                                                                                                                                                 <  2:932859/150‑1 (MQ=255)
         aCAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAggggg                                                                                                                                             <  2:796759/150‑1 (MQ=255)
          cAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGa                                                                                                                                            <  1:937548/150‑1 (MQ=255)
           aGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGaa                                                                                                                                           >  2:948558/1‑150 (MQ=255)
            gCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAg                                                                                                                                          <  1:492981/150‑1 (MQ=255)
                 tggggAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAATCGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTc                                                                                                                                     >  2:217662/4‑150 (MQ=255)
                   aGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCTGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCTGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAg                                                                                                                                   >  2:1029024/1‑150 (MQ=255)
                    ggAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGGGCCGGTGCGCCGGCTCTGAgg                                                                                                                                                <  2:652552/136‑1 (MQ=255)
                    ggAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGGGCCGGTGCGCCGGCTCTGAgg                                                                                                                                                >  1:652552/1‑136 (MQ=255)
                     gagaAATCCAGCGTTGGCCTGTAAACCATTAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTa                                                                                                                                 <  1:602159/150‑1 (MQ=255)
                       gaAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACt                                                                                                                               <  1:984339/150‑1 (MQ=255)
                        aaaTCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTc                                                                                                                              <  1:714692/150‑1 (MQ=255)
                         aaTCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTcc                                                                                                                             <  1:803267/150‑1 (MQ=255)
                              aGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCagaa                                                                                                                        <  2:127141/150‑1 (MQ=255)
                                    ggCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCg                                                                                                                  >  1:819730/1‑150 (MQ=255)
                                        tGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAgg                                                                                                                                                >  1:765162/1‑116 (MQ=255)
                                        tGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAgg                                                                                                                                                <  2:765162/116‑1 (MQ=255)
                                         gTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCa                                                                                                             >  2:1217282/1‑150 (MQ=255)
                                          tAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAg                                                                                                            >  2:164615/1‑150 (MQ=255)
                                                  gAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCa                                                                                                                           <  2:330723/127‑1 (MQ=255)
                                                  gAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCa                                                                                                                           >  1:330723/1‑127 (MQ=255)
                                                     gCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGt                                                                                                 <  2:547679/150‑1 (MQ=255)
                                                            aGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATcc                                                                                                                   <  2:1228442/125‑1 (MQ=255)
                                                            aGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGCGGAAGCACTCAGTACTCCCAGAAGATcc                                                                                                                   >  1:1228442/1‑125 (MQ=255)
                                                             gAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTg                                                                                                     >  1:1010366/1‑138 (MQ=255)
                                                             gAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTg                                                                                                     <  2:1010366/138‑1 (MQ=255)
                                                             gAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTc                                                                                         >  1:677835/1‑150 (MQ=255)
                                                              aaaGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGGGCCGGTGCGCCGGCTCTGAgg                                                                                                                                                >  1:1195340/1‑94 (MQ=38)
                                                              aaaGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGGGCCGGTGCGCCGGCTCTGAgg                                                                                                                                                <  2:1195340/94‑1 (MQ=38)
                                                              aaaGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTc                                                                                         <  1:508921/149‑1 (MQ=255)
                                                              aaaGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTc                                                                                         >  2:508921/1‑149 (MQ=255)
                                                              aaaGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCt                                                                                        >  1:1077105/1‑150 (MQ=255)
                                                                aGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCTGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTg                                                                                      <  1:1029024/150‑1 (MQ=255)
                                                                aGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGGAGGGGGGGGGGGGGGGGGGGGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTg                                                                                      <  2:105064/150‑1 (MQ=255)
                                                                  gTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGt                                                                                    >  2:20534/1‑150 (MQ=255)
                                                                     aaCGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATcc                                                                                                                   <  1:490172/116‑1 (MQ=255)
                                                                     aaCGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATcc                                                                                                                   >  2:490172/1‑116 (MQ=255)
                                                                       cGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCg                                                                               >  2:135073/1‑150 (MQ=255)
                                                                        gATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGc                                                                              >  2:697895/1‑150 (MQ=255)
                                                                              gCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTTGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAACAAACGCAGAAGATCCGCTGCAGTGCTTTTCTGTCCTGGGTCTTGGTTATCGCTACCAt                                                                        >  1:978911/1‑150 (MQ=255)
                                                                              gCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTg                                                                                                                                                   <  1:1185312/75‑1 (MQ=255)
                                                                              gCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTg                                                                                                                                                   >  2:1185312/1‑75 (MQ=255)
                                                                                    ttGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAg                                                                  >  1:567255/1‑150 (MQ=255)
                                                                                            tctGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCAt                                                                                 >  2:370301/1‑127 (MQ=255)
                                                                                            tctGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCAt                                                                                 <  1:370301/127‑1 (MQ=255)
                                                                                             ctGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCgcgc                                                         >  1:1166919/1‑150 (MQ=255)
                                                                                                 gtCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGGACTCAGTa                                                                                                                                 >  2:921735/1‑74 (MQ=255)
                                                                                                 gtCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTa                                                                                                                                 <  1:921735/74‑1 (MQ=255)
                                                                                                  tCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACt                                                                                                                                      <  2:117108/68‑1 (MQ=255)
                                                                                                  tCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACt                                                                                                                                      >  1:117108/1‑68 (MQ=255)
                                                                                                    tACGCGGTTGTGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAACCACTCAGTACGCCCAGAAGATCCGCTGCAGCTGTTTGCTGTCCTGGGACTTGGTCATCGGTACCATCGCCAGGATCCGCGCTTTTTgt                                                  >  2:687928/1‑149 (MQ=255)
                                                                                                      cgcgGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCgg                                                <  2:677835/150‑1 (MQ=255)
                                                                                                      cgcgGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCgg                                                <  1:948558/150‑1 (MQ=255)
                                                                                                          gTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTc                                            >  2:1033392/1‑150 (MQ=255)
                                                                                                           ttGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCa                                           <  2:1077105/150‑1 (MQ=255)
                                                                                                            tGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAg                                          <  1:135073/150‑1 (MQ=255)
                                                                                                             gAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCt                                                                             <  1:1023652/114‑1 (MQ=255)
                                                                                                             gAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCt                                                                             >  2:1023652/1‑114 (MQ=255)
                                                                                                             gAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAgg                                         >  1:559427/1‑150 (MQ=255)
                                                                                                               ggAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTc                                       >  1:645444/1‑150 (MQ=255)
                                                                                                                 aaTTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGt                                     >  2:985407/1‑150 (MQ=255)
                                                                                                                 aaTTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGt                                     <  1:697895/150‑1 (MQ=255)
                                                                                                                   ttGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGa                                   <  2:819730/150‑1 (MQ=255)
                                                                                                                      gCAACTAGGCGTTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGcc                                <  1:217662/150‑1 (MQ=255)
                                                                                                                           ttcccccTCAGGGCCGGTGCGCCGGCTCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGAc                           >  2:284389/5‑150 (MQ=255)
                                                                                                                              gCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCa                        >  2:239826/1‑150 (MQ=255)
                                                                                                                                ccTCAGGGCCGGTGCGCCGGCTCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGt                      >  2:289841/1‑150 (MQ=255)
                                                                                                                                ccTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTgg                                                                                            >  2:1208311/1‑80 (MQ=255)
                                                                                                                                ccTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTgg                                                                                            <  1:1208311/80‑1 (MQ=255)
                                                                                                                                ccTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCa                        >  1:498942/1‑148 (MQ=255)
                                                                                                                                ccTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCa                        <  2:498942/148‑1 (MQ=255)
                                                                                                                                ccTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATACGCGATTGTTGCGGGTTCAGGTCGTGAGCCACGACCCAGt                      >  2:621897/1‑150 (MQ=255)
                                                                                                                                        ccGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTg              <  2:567255/150‑1 (MQ=255)
                                                                                                                                        ccGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTg              <  1:1278936/150‑1 (MQ=255)
                                                                                                                                                 cGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGc     <  2:1166919/150‑1 (MQ=255)
                                                                                                                                                    cccTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCTCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGt  >  1:951737/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AAATTCGCAACAGCTTGTCAGGAGAAATCCAGCGTTGGCCTGTAAACCATGAGGCCGGTCAGAAAGGTAAACGATGTCGCCGGTTTGTAACCTCTGTGTCTACGCGGTTGAGGAATTGGCAACGAGGCCCTCAGAGCCGGCGCACCGGCCCTGAGGGGGAAGCACTCAGTACTCCCAGAAGATCCGCTGCAGCTCTTTGCTGTCCTGGGTCTTGGTCATCGCTACCATCGCCAGGATCCGCGCTTTTTGCGGGTTCAGGTCGTGAGCCACGACCCAGTCGTTCTTGTCATCCGGCTGT  >  NC_002947/2799080‑2799377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑