Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,812,783 T→C 11.7% intergenic (+34/‑76) rplT → / → pheS 50S ribosomal protein L20/phenylalanine‑‑tRNA ligase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,812,7830TC11.7% 132.0 / 7.5 51intergenic (+34/‑76)rplT/pheS50S ribosomal protein L20/phenylalanine‑‑tRNA ligase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (22/23);  new base C (3/3);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

TGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTG  >  NC_002947/2812640‑2812930
                                                                                                                                               |                                                                                                                                                   
tGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCGCCCGGCAGCGCTAGCACCGCCggg                                                                                                                                               <  1:32490/150‑1 (MQ=255)
 gAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGc                                                                                                                                              >  2:672438/1‑150 (MQ=255)
  aaaaaGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCa                                                                                                                                             >  2:1090302/1‑150 (MQ=255)
   aaaaGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCAt                                                                                                                                            >  1:84867/1‑150 (MQ=255)
         cTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGTGGTGCTAGCGCTGCCGGGTATTGAACg                                                                                                                                      <  1:162411/150‑1 (MQ=255)
             gatcgaAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCAt                                                                                                                                  >  2:543075/1‑150 (MQ=255)
                cgaAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAACTAGCGAAGTGTGCGGCGATTGTCGAGAAAGCTCAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGa                                                                                                                               <  1:607390/150‑1 (MQ=255)
                cgaAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCGCCCGGCAGCGCTAGCACCGCCgggtga                                                                                                                                            <  2:480206/137‑4 (MQ=255)
                cgaAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCGCCCGGCAGCGCTAGCACCGCCgggtga                                                                                                                                            >  1:480206/1‑134 (MQ=255)
                   aaTCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAg                                                                                                                            <  1:312156/150‑1 (MQ=255)
                    aTCGACCGTAAGGTTCTGGCTGAACTGGTAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCGGTTCTGGCTTAAGTACCCGCGACCTGCACCGCGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAgg                                                                                                                           <  1:543075/150‑1 (MQ=255)
                             aaGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGcc                                                                                                                  <  1:679127/150‑1 (MQ=255)
                                               gCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTa                                                                                                <  1:544723/150‑1 (MQ=255)
                                                 aGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTAtt                                                                                              >  1:60011/1‑150 (MQ=255)
                                                    gAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTc                                                                                           <  1:113438/150‑1 (MQ=255)
                                                               gcgcgTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCTACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACCTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGt                                                                                <  2:460762/148‑1 (MQ=255)
                                                                 gcgTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCt                                                                              <  2:839012/150‑1 (MQ=255)
                                                                  cgTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGACCCTTATATGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTg                                                                             <  1:91593/150‑1 (MQ=255)
                                                                                   gagaAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGa                                                            >  1:1022674/1‑150 (MQ=255)
                                                                                    agaAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCAGCGCTAGCACCGCCgggtg                                                                                                                                             <  2:372700/68‑3 (MQ=21)
                                                                                    agaAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCAGCGCTAGCACCGCCgggtg                                                                                                                                             >  1:372700/1‑66 (MQ=25)
                                                                                      aaaGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTa                                                                           >  2:947273/1‑132 (MQ=255)
                                                                                      aaaGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTa                                                                           <  1:947273/132‑1 (MQ=255)
                                                                                      aaaGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATgc                                                         >  2:850751/1‑150 (MQ=255)
                                                                                         gCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCt                                                      >  2:459851/1‑150 (MQ=255)
                                                                                          cTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTg                                                     <  1:1090302/150‑1 (MQ=255)
                                                                                           tAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTgg                                                    >  1:698213/1‑150 (MQ=255)
                                                                                            aaaGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCTTTGAAGGCTCTTcccc                                                                                                  >  2:1082828/1‑103 (MQ=38)
                                                                                            aaaGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCTTTGAAGGCTCTTcccc                                                                                                  <  1:1082828/103‑1 (MQ=255)
                                                                                                ccGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTcc                                               <  1:459851/150‑1 (MQ=255)
                                                                                                   ttCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCaa                                            >  2:647525/1‑150 (MQ=255)
                                                                                                        gCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCt                                       >  1:761099/1‑150 (MQ=255)
                                                                                                         cTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCCTCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGCTCTCCCAAGACCtc                                      >  2:229691/1‑149 (MQ=255)
                                                                                                          ttAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTag                                     >  1:531838/1‑150 (MQ=255)
                                                                                                           tAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTaga                                    <  2:84867/150‑1 (MQ=255)
                                                                                                           tAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTaga                                    <  1:850751/150‑1 (MQ=255)
                                                                                                                  ccGCGACAATCACCCGGTGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTg                             <  1:915707/150‑1 (MQ=255)
                                                                                                                  ccGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTg                             >  2:416193/1‑150 (MQ=255)
                                                                                                                  ccGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCAATAGGGGAAGAGCTTTGAAGGCTCTTCCCCTAtcga                                                                                            >  1:95822/1‑84 (MQ=25)
                                                                                                                  ccGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCAATAGGGGAAGAGCTTTGAAGGCTCTTCCCCTAtcga                                                                                            <  2:95822/87‑4 (MQ=21)
                                                                                                                    gcgACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGa                           >  2:142085/1‑150 (MQ=255)
                                                                                                                      gACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAAc                         <  1:416193/150‑1 (MQ=255)
                                                                                                                        cAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAAcgc                       >  2:731293/1‑150 (MQ=255)
                                                                                                                           atgcccGGCAGCGCTAGCACCGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCGCCTATTTTCGTATCTGGAGTCTGTACATGGAACAGCGGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCt                    >  1:1239137/4‑150 (MQ=255)
                                                                                                                             aCCCGGCGGTGCTAGCGCTTCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCCGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGa                  >  1:1208103/1‑150 (MQ=255)
                                                                                                                              cccGGCGGTGCTAGCGCTTCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGaa                 <  2:1208103/150‑1 (MQ=255)
                                                                                                                               ccGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAggg                                                                                                                          >  2:160027/1‑44 (MQ=255)
                                                                                                                               ccGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAggg                                                                                                                          <  1:160027/44‑1 (MQ=255)
                                                                                                                               ccGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAg                >  1:182839/1‑150 (MQ=255)
                                                                                                                                             gcTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTg  <  2:531838/150‑1 (MQ=255)
                                                                                                                                             gcTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTg  <  1:672438/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
TGAAAAAGGCTTCGATCGAAATCGACCGTAAGGTTCTGGCTGATCTGGCAGTGAACGAAAAAGCGGCGTTTGCTGCGATTGTCGAGAAAGCTAAAGCCGTTCTGGCTTAAGTACCCGCGACAATCACCCGGCGGTGCTAGCGCTGCCGGGCATTGAACGTCATCGATAGGGGAAGAGCCTTCAAAGCTCTTCCCCTATTTTCGTATCTGGAGTCTGTACATGGAAAACCTGGATGCGCTGGTCTCCCAAGCCCTAGAGGCCGTGGAACGCGCTGAAGACATCAACGCCCTG  >  NC_002947/2812640‑2812930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑