Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,879,625 G→A 8.5% G161G (GGC→GGT)  PP_2534 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,879,6250GA 8.5% 131.9 / 2.7 47G161G (GGC→GGT) PP_2534LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (22/21);  new base A (2/2);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

CCGTCGCGGTGAAACGGCCAGTCAGGCATCGCGCGGCGGAAGTTGAAGCGCAGGCAGTTGTGCCTGGCAAGGTCTTCAGGTTCAAGCGGGGTACCGTAGCGTTGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGAAGCGCAGCGCCACGTCGGCCTGGCCGCCGAGTATATCGGCCACCTCGTCGCTGAGGGTCAGGTCAACCAGCACTTGCGGGTAGCGTGCGCTGAAGGCCGCC  >  NC_002947/2879482‑2879769
                                                                                                                                               |                                                                                                                                                
ccGTCGCGGTGAAACGGCCAGTCAGGCATCGCGCGGCGGAAGTTGAAGCGCAGGCAGTTGTGCCTGGCAAGGTCTTCAGGTTCAAGCGGGGTACCGTAGCGTTGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATg                                                                                                                                            <  1:153205/150‑1 (MQ=255)
ccGTCGCGGTGAAACGGCCAGTCAGGCATCGCGCGGCGGAAGTTGAAGCGCAGGCAGTTGTGCCTGGCAAGGTCCCCACGTTCAAGCGGGGTACCGTAGCGTTGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATg                                                                                                                                            >  1:409199/1‑150 (MQ=255)
     gcgGTGAAACGGCCAGTCAGGCATCGCGCGGCGGAAGTTGAAGCGCAGGCAGTTGTGCCTGGCAAGGTCTTCAGGTTCAAGCGGGGTACCGTAGCGTTGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCACCAATGgcgcg                                                                                                                                       >  1:372380/1‑150 (MQ=255)
               ggCCAGTCAGGCATCGCGCGGCGGAAGTTGAAGCGCAGGCAGTTGTGCCTGGCAAGGTCTTCAGGTTCAAGCGGGGTACCGTAGCGTTGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAAc                                                                                                                             >  2:1252977/1‑150 (MQ=255)
                  cAGTCAGGCATCGCGCGGCGGAAGTTGAAGCGCAGGCAGTTGTGCCTGGCAAGGTCTTCAGGTTCAAGCGGGGTACCGTAGCGTTGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAg                                                                                                                          <  1:1190367/150‑1 (MQ=255)
                       aGGCATCGCGCGGCGGAAGTTGAAGCGCAGGCAGTTGTGCCTGGCAAGGTCTTCAGGTTCAAGCGGGGTACCGTAGCGTTGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTc                                                                                                                     >  2:449246/1‑150 (MQ=255)
                       aGGCATCGCGCGGCGGAAGTTGAAGCGCAGGCAGTTGTGCCTGGCAAGGTCTTCAGGTTCAAGCGGGGTACCGTAGCGTTGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTc                                                                                                                     <  1:449246/150‑1 (MQ=255)
                          cATCGCGCGGCGGAAGTTGAAGCGCAGGCAGTTGTGCCTGGCAAGGTCTTCAGGTTCAAGCGGGGTACCGTAGCGTTGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGa                                                                                                                           <  2:837386/141‑1 (MQ=255)
                          cATCGCGCGGCGGAAGTTGAAGCGCAGGCAGTTGTGCCTGGCAAGGTCTTCAGGTTCAAGCGGGGTACCGTAGCGTTGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGa                                                                                                                           >  1:837386/1‑141 (MQ=255)
                               cgcgGCGGAAGTTGAAGCGCAGGCAGTTGTGCCTGGCAAGGTCTTCAGGTTCAAGCGGGGTACCGTAGCGTTGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGt                                                                                                             <  2:97694/150‑1 (MQ=255)
                                  ggcggAAGTTGAAGCGCAGGCAGTTGTGCCTGGCAATGTCTTCAGGTTCAAGCGGGGTACCGTAGCGTTGCAGGTATTCTGGCGAGGCCACACCTAGGTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGAc                                                                                                          <  1:230124/150‑1 (MQ=255)
                                           tGAAGCGCAGGCAGTTGTGCCTGGCAAGGTCTTCAGGTTCAAGCGGGGTACCGTAGCGTTGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGAagcgca                                                                                                 <  1:883103/150‑1 (MQ=255)
                                            gAAGCGCAGGCAGTTGTGCCTGGCAAGGTCTTCAGGTTCAAGCGGGGTACCGTAGCGTTGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGAagcgcag                                                                                                >  1:410890/1‑150 (MQ=255)
                                            aaaGCGCAGGCAGTTGTGCCTGGCAAGGTCTTCAGGTTACATCGGGGTACCGTAGCGTTGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGAagcgcag                                                                                                <  1:11421/149‑1 (MQ=255)
                                               gcgcAGGCAGTTGTGCCTGGCAAGGTCTTCAGGTTCAAGCGGGGTACCGTAGCGTTGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCa                                                                                                                <  1:1130159/131‑1 (MQ=255)
                                               gcgcAGGCAGTTGTGCCTGGCAAGGTCTTCAGGTTCAAGCGGGGTACCGTAGCGTTGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCa                                                                                                                >  2:1130159/1‑131 (MQ=255)
                                                       agTTGTGCCTGGCAAGGTCTTCAGGTTCAAGCGGGGTACCGTAGCGTTGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGAAGCGCAGCGCCACGTCgg                                                                                     >  2:836685/1‑150 (MQ=255)
                                                            tgCCTGGCAAGGTCTTCAGGTTCAAGCGGGGTACCGTAGCGTTGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGAAGCGCAGCGCCACGTCGGCCTgg                                                                                >  1:235274/1‑150 (MQ=255)
                                                                 ggCAAGGTCTTCAGGTTCAAGCGGGGTACCGTAGCGTTGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGAAGCGCAGCGCCACGTCGGCCTGgccgcc                                                                           <  1:1194460/150‑1 (MQ=255)
                                                                     aGGTCTTCAGGTTCAAGCGGGGTACCGTAGCGTTGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCACCAATGGCGCGCGCGCTCAACGCGCTGTCCGGCAGGTGACCGAAGCGCAGCGCCACGTCGGCCTGTCCGCCGAGt                                                                       <  2:372380/150‑1 (MQ=255)
                                                                           tCAGGTTCAAGCGGGGTACCGTAGCGTTGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGAAGCGCAGCGCCACGTCGGCCTGGCCGCCGAGTATATCg                                                                 <  1:1252977/150‑1 (MQ=255)
                                                                             aGGTTCAAGCGGGGTACCGTAGCGTTGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGAAGCGCAGCGCCACGTCGGCCTGGCCGCCGAGTATATCGGc                                                               <  2:410890/150‑1 (MQ=255)
                                                                               gTTCAGGCGGGGTACCGTAGCGTTGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGAAGCGCAGCGCCACGTCGGCCTGGCCGCCGAGTATATCGGCCa                                                             >  1:748874/1‑150 (MQ=255)
                                                                               gTTCAAGCGGGGTACCGTAGCGTTGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCACCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGt                                                                                                             >  1:419733/1‑102 (MQ=255)
                                                                               gTTCAAGCGGGGTACCGTAGCGTTGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCACCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGt                                                                                                             <  2:419733/102‑1 (MQ=255)
                                                                               gTTCAAGCGGGGTACCGTAGCGGTGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGAAGCGCAGCGCCACGTCGGCCTGGCCGCCGAGTATATCGGCCa                                                             >  1:477366/1‑150 (MQ=255)
                                                                                            aCCGTAGCGTTGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGAAGCGCAGCGCCACGTCGGCCTGGCCGCCGAGTATATCGGCCACCTCGTCGCTGAg                                                <  2:235274/150‑1 (MQ=255)
                                                                                                     ttGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGgcgc                                                                                                                                        <  1:790471/53‑1 (MQ=255)
                                                                                                     ttGCAGGTATTCTGGCGAGGCCACCACTACCGGGCCGGTCTCGCCAATGgcgc                                                                                                                                        >  2:790471/1‑53 (MQ=255)
                                                                                                      tGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGAAGCGCAGCGCCACGTCGGCCTGGCCGCCGAGTATATCGGCCACCTCGTCGCTGAGggtcaggtca                                      >  1:523995/1‑150 (MQ=255)
                                                                                                       gCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGAAGCGCAGCGCCACGTCGGCCTGGccgccgtg                                                                        <  1:289481/115‑3 (MQ=255)
                                                                                                       gCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGAAGCGCAGCGCCACGTCGGCCTGGccgccgtg                                                                        >  2:289481/1‑113 (MQ=255)
                                                                                                          ggTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGAAGCGCAGCGcc                                                                                            >  1:200851/1‑92 (MQ=255)
                                                                                                          ggTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGAAGCGCAGCGcc                                                                                            <  2:200851/92‑1 (MQ=255)
                                                                                                           gTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGAagc                                                                                                    <  2:222502/83‑1 (MQ=255)
                                                                                                           gTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGAagc                                                                                                    >  1:222502/1‑83 (MQ=255)
                                                                                                            tATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGAAGCGCAGCGCCACGTCGGCCTGGCCGCCGAGTATATCGGCCACCTCGTCGCTGAGGGTCAGGTCAACCAGc                                >  1:109810/1‑150 (MQ=255)
                                                                                                              ttCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGAAGCGCAGCGCCACGTCGGCCTGGCCGCCGAGTCAATGGGCCACCTCGTCGCTGAGGGTCAGGTCAACCAGCAc                              >  1:11884/1‑150 (MQ=255)
                                                                                                              ttCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGAAGCGCAGCGCCACGTCGGCCTGGCCGCCGAGTATATCGGCCACCTCGTCGCTGAGGGTCAGGTCAACCAGCAc                              >  2:635585/1‑150 (MQ=255)
                                                                                                                   gCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGAAGCGCAGCGCCACGTCGGCCTGGCCGCCGAGTATATCGGCCACCTCGTCGCTGAGGGTCAGGTCAACCAGCACTTGCg                         <  2:477366/150‑1 (MQ=255)
                                                                                                                      aGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGaa                                                                                                      >  2:193640/1‑70 (MQ=255)
                                                                                                                      aGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGaa                                                                                                      <  1:193640/70‑1 (MQ=255)
                                                                                                                               cTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGAAGCGCAGCGCCACGTCGGCCTGGCCGCCGAGTATATCGGCCACCTCGTCGCTGAGGGTCAGGTCAACCAGCACTTGCGGGTAGCGTgcgc             >  2:427882/1‑150 (MQ=255)
                                                                                                                                  ccTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGAAGCGCAGCGCCACGTCGGCCTGGCCGCCGAGTATATCGGCCACCTCGTCGCTGAGGGTCAGGTCAACCAGCACTTGCGGGTAGCGTGCGCTGa          >  2:703417/1‑150 (MQ=255)
                                                                                                                                   cTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGAAGCGCAGCGCCACGTCGGCCTGGCCGCCGAGTATATCGGCCACCTCGTCGCTGAGGGTCAGGTCAACCAGCACTTGCGGGTAGCGTGCGCTGaa         <  1:736803/150‑1 (MQ=255)
                                                                                                                                      gCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGAAGCGCAGCGCCACGTCGGCCTGGCCGCCGAGTATATCGGCCACCTCGTCGCTGAGGGTCAGGTCAACCAGCACTTGCGGGTAGCGTGCGCTGAAGgc      <  2:409199/150‑1 (MQ=255)
                                                                                                                                          gTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGAAGCGCAGCGCCACGTCGGCCTGGCCGCCGAGTATATCGGCCACCTCGTCGCTGAGGGTCAGGTCAACCAGCACTTGCGGGTAGCGTGCGCTGAAGgccgcc  >  1:430857/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                
CCGTCGCGGTGAAACGGCCAGTCAGGCATCGCGCGGCGGAAGTTGAAGCGCAGGCAGTTGTGCCTGGCAAGGTCTTCAGGTTCAAGCGGGGTACCGTAGCGTTGCAGGTATTCTGGCGAGGCCACCACTACCTGGCCGGTCTCGCCAATGGCGCGCGCGCTCAACGAGCTGTCCGGCAGGTGACCGAAGCGCAGCGCCACGTCGGCCTGGCCGCCGAGTATATCGGCCACCTCGTCGCTGAGGGTCAGGTCAACCAGCACTTGCGGGTAGCGTGCGCTGAAGGCCGCC  >  NC_002947/2879482‑2879769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑