Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 230,863 C→T 5.5% A175V (GCG→GTG)  PP_0179 → putative efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947230,8630CT 5.5% 207.3 / 2.2 73A175V (GCG→GTG) PP_0179putative efflux transporter
Reads supporting (aligned to +/- strand):  ref base C (32/37);  new base T (2/2);  total (34/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.88e-01

GGTGCCGGTAGCCAACATCGGTGAGGCCATCATCAGTGCTTCGTACCAGTTCGACCTGTGGGGCACCTTCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCGATTTGCAGGAGCAAAGCGTGAAACTCAACCAGCGCCTGCGCGATGCTGGGCGTGGCGATGAAACCCAGGTCACTCGC  >  NC_002947/230714‑231011
                                                                                                                                                     |                                                                                                                                                    
ggTGCCGGTAGCCAACATCGGTGAGGCCATCATCAGTGCTTCGTACCAGTTCGACCTGTGGGGCACCTTCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGc                                                                                                                                                      <  2:634235/150‑1 (MQ=255)
        tAGCCAACATCGGTGAGGCCATCATCAGTGCTTCGTACCAGTTCGACCTGTGGGGCACCTTCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTgg                                                                                                                                              <  1:620311/150‑1 (MQ=255)
            cAACATCGGTGAGGCCATCATCAGTGCTTCGTACCAGTTCGACCTGTGGGGCACCTTCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCaa                                                                                                                                          >  2:141220/1‑150 (MQ=255)
            aaaCATCGGTGAGGCCATCATCAGTGCTTCGTACCAGTTCGACCTGTGGGGCACCTTCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCCGTGCAGGCCGCTGCCGACACTGACCGCATCACGCTTGTGGCGGACGTGGTCaa                                                                                                                                          <  2:873000/149‑1 (MQ=255)
             aaCATCGGTGAGGCCATCATCAGTGCTTCGTACCAGTTCGACCTGTGGGGCACCTTCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAg                                                                                                                                         >  2:167128/1‑150 (MQ=255)
              aCATCGGTGAGGCCATCATCAGTGCTTCGTACCAGTTCGACCTGTGGGGCACCTTCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAgg                                                                                                                                        <  2:574513/150‑1 (MQ=255)
               cATCGGTGAGGCCATCATCAGTGCTTCGTACCAGTTCGACCTGTGGGGCACCTTCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGc                                                                                                                                       <  2:642514/150‑1 (MQ=255)
               cATCGGTGAGGCCATCATCAGTGCTTCGTACCAGTTCGACCTGTGGGGCACCTTCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGc                                                                                                                                       >  2:416790/1‑150 (MQ=255)
                    gTGAGGCCATCATCAGTGCTTCGTACCAGTTCGACCTGTGGGGCACCTTCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTtata                                                                                                                                  <  1:416790/150‑1 (MQ=255)
                         gCCATCATCAGTGCTTCGTACCAGTTCGACCTGTGGGGCACCTTCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATAcaccc                                                                                                                             <  2:216914/150‑5 (MQ=255)
                         gCCATCATCAGTGCTTCGTACCAGTTCGACCTGTGGGGCACCTTCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAg                                                                                                                             <  1:141220/150‑1 (MQ=255)
                         gCCATCATCAGTGCTTCGTACCAGTTCGACCTGTGGGGCACCTTCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAg                                                                                                                             <  2:434852/150‑1 (MQ=255)
                          ccATCATCAGTGCTTCGTACCAGTTCGACCTGTGGGGCACCTTCAAGCGTGGTACAGAGGCTGCAAAGACAAATGCCGACGCGGTGCAGGCCGCTGCCCACACTGCCCGCATCACGCTCGTGGCGGACGCGGGCAAGGCTTATACCCAgg                                                                                                                            >  2:410691/1‑150 (MQ=255)
                                 caGTGCTTCGTACCAGTTCGACCTGTGGGGCACCTTCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTc                                                                                                                     <  1:201996/150‑1 (MQ=255)
                                          gTACCAGTTCGACCTGTGGGGCACCTTCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGa                                                                                                            >  2:764802/1‑150 (MQ=255)
                                           tACCAGTTCGACCTGTGGGGCACCTTCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAg                                                                                                           >  1:1240970/1‑150 (MQ=255)
                                             ccAGTTCGACCTGTGGGGCACCTTCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGACCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGa                                                                                                         >  2:327857/1‑150 (MQ=255)
                                                   cGACCTGTGGGGCACCTTCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACca                                                                                                   <  1:764802/150‑1 (MQ=255)
                                                       cTGTGGGGCACCTTCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACca                                                                                                   >  1:238162/1‑146 (MQ=255)
                                                       cTGTGGGGCACCTTCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACca                                                                                                   <  2:238162/146‑1 (MQ=255)
                                                        tgtgGGGCACCTTCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCa                                                                                                                                           <  2:229207/105‑1 (MQ=255)
                                                        tgtgGGGCACCTTCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCa                                                                                                                                           >  1:229207/1‑105 (MQ=255)
                                                           ggggCACCTTCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTta                                                                                                                                    <  1:629510/109‑1 (MQ=255)
                                                           ggggCACCTTCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTta                                                                                                                                    >  2:629510/1‑109 (MQ=255)
                                                                 ccTTCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGt                                                                                     <  1:371363/150‑1 (MQ=255)
                                                                  cTTCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGt                                                                                         >  2:248972/1‑145 (MQ=255)
                                                                  cTTCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGt                                                                                         <  1:248972/145‑1 (MQ=255)
                                                                  cTTCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTc                                                                                    >  2:127080/1‑150 (MQ=255)
                                                                   ttCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACAtt                                                                                               >  2:1170654/1‑138 (MQ=255)
                                                                   ttCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACAtt                                                                                               <  1:1170654/138‑1 (MQ=255)
                                                                   ttCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTcc                                                                                   >  1:1154488/1‑150 (MQ=255)
                                                                      aaGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTc                                                                                <  2:299099/150‑1 (MQ=255)
                                                                       aGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCg                                                                               >  2:148520/1‑150 (MQ=255)
                                                                       aGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGCACGTGGACAAGGCTTATACCCAGCTGTGCTCGCCCAACGAGGAATACCACATTGCCCGTGAGTCGCTCg                                                                               >  2:570931/1‑150 (MQ=255)
                                                                           tGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTTAGCCCCTCGAttt                                                                           >  2:869136/1‑150 (MQ=255)
                                                                            ggTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCGCACATCACGCTTGTGGCGGACGCGGTCAAGGCTTAAACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCGAtttt                                                                          >  2:382876/1‑149 (MQ=255)
                                                                                ccGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCGATTTGCAgg                                                                      >  2:1062629/1‑150 (MQ=255)
                                                                                  gAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCGATTTGCAGGAg                                                                    <  1:297130/150‑1 (MQ=255)
                                                                                      cTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCGATTTGCAGGAGCaaa                                                                <  1:148520/150‑1 (MQ=255)
                                                                                        gCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCTGACGTGGTCCAGGCGTCTACCCAGGTGTGCTCGGCTAACGAGGAATACCACATTGCCCGTGAGTCCCTCGATTTGCAGGAGCAAAGc                                                              <  1:410691/150‑1 (MQ=255)
                                                                                         ccAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTccc                                                                                  <  1:857111/129‑1 (MQ=255)
                                                                                         ccAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTccc                                                                                  >  2:857111/1‑129 (MQ=255)
                                                                                         ccAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCGATTTGCAGGAGCAAAGCg                                                             <  2:1240970/150‑1 (MQ=255)
                                                                                         ccAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCGATTTGCAGGAGCAAAGCg                                                             >  1:892547/1‑150 (MQ=255)
                                                                                              gCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCGATTTGCAGGAGCAAAGCGTGaaa                                                        <  1:869136/150‑1 (MQ=255)
                                                                                                   tGCCGACGCGGTGCAGACCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCGATTTGCAGGAGCAAAGCGTGAAACTCaa                                                   <  1:327857/150‑1 (MQ=255)
                                                                                                       gACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTc                                                                                                                     <  2:30873/80‑1 (MQ=255)
                                                                                                       gACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTc                                                                                                                     >  1:30873/1‑80 (MQ=255)
                                                                                                        aCGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCGATTTGCAGGAGCAAAGCGTGAAACTCAACCAgc                                              >  1:459121/1‑150 (MQ=255)
                                                                                                            ggTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCGATTTGCAGGAGCAAAGCGTGAAACTCAACCAGCGCCt                                          <  2:805550/150‑1 (MQ=255)
                                                                                                             gTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATAc                                                                                                     <  1:389027/90‑1 (MQ=255)
                                                                                                             gTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATAc                                                                                                     >  2:389027/1‑90 (MQ=255)
                                                                                                                   gCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGCATAGCACATTGCCCGTGAGTCCCTCGATTTGCAGGAGCAAAGCGTGAAAATCAACCAGCGCCTGCGCGAt                                   >  1:541845/1‑150 (MQ=255)
                                                                                                                           cGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCGATTTGCAGGAGCAAAGc                                                              >  2:98916/1‑115 (MQ=255)
                                                                                                                           cGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCGATTTGCAGGAGCAAAGc                                                              <  1:98916/115‑1 (MQ=255)
                                                                                                                           cGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCGATTTGCAGGAGCAAAGCGTGAAACTCAAc                                                  >  2:399510/1‑127 (MQ=255)
                                                                                                                           cGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCGATTTGCAGGAGCAAAGCGTGAAACTCAAc                                                  <  1:399510/127‑1 (MQ=255)
                                                                                                                            gACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGATTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCGATTTGCAGGAGCAAAGCGTGAAACTCAACCAGCGCCTGCGCGATGCTGGGCGt                          >  1:1224959/1‑150 (MQ=255)
                                                                                                                              cacTGCCCGCATCCCGCTTGTGGCGGCCGTGGTCAAGGCTTATACCCAGGGGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCGATTTGCAGGAGCAAAGCGTGAAACTCAACCAGCGCCTGCGCGATGCTGGGCGTgg                        <  1:570931/150‑1 (MQ=255)
                                                                                                                              cacTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCGATTTGCAGGAGCAAAGCGTGAAACTCAACCAGCGCCTGCGCGATGCTGGGCGTgg                        <  1:127080/150‑1 (MQ=255)
                                                                                                                              cacTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCGATTTGCAGGAGCAAAGCGTGAAACTCAACCAGCGCCTGCGCGATGCTGGGCGTgg                        >  2:879005/1‑150 (MQ=255)
                                                                                                                              cacTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCGATTTGCAGGAGCAAAGCGTGAAACTCAACCAGCGCCTGCGCGATGCTGGGCGTgg                        >  2:3671/1‑150 (MQ=255)
                                                                                                                                  gctcGCATCCCGCGTGTGGCGGACGTCGTCAAGGCTTACATCCAGGTGTGCTCGGCCAACGAGGAATGCCACATTGCCCGTGAGGCCCTCGATTTGCAGGAGCAGAGCGTGAAACTCAACCAGCGCCTGCGCGATGCTGGGCGTGGCGAt                    <  2:408359/147‑1 (MQ=255)
                                                                                                                                    ccGCATCACGCTTGTGGCGGACGTGGTCAACCCTCAAACATCCGCATGCTGCGGCATCGAGGAATACCACATAGCCCGTGAGTCCCTCGATTTGCAGGAGCAAAGCGTGAAACTAAACCAGCGCCTGCTCGATGCTGGGCGTGGCGATGa                  >  1:223425/1‑150 (MQ=255)
                                                                                                                                      gCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGgt                                                                                                                           <  1:128657/43‑1 (MQ=255)
                                                                                                                                      gCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGgt                                                                                                                           >  2:128657/1‑43 (MQ=255)
                                                                                                                                          cACGCTTGTGGTGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCGATTTGCAGGAGCAAAGCGTGAAACTCAACCAGCGCCTgcgc                                      >  2:625707/1‑124 (MQ=255)
                                                                                                                                          cACGCTTGTGGTGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCGATTTGCAGGAGCAAAGCGTGAAACTCAACCAGCGCCTgcgc                                      <  1:1269133/124‑1 (MQ=255)
                                                                                                                                          cACGCTTGTGGTGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCGATTTGCAGGAGCAAAGCGTGAAACTCAACCAGCGCCTgcgc                                      >  2:1269133/1‑124 (MQ=255)
                                                                                                                                          cACGCTTGTGGTGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCGATTTGCAGGAGCAAAGCGTGAAACTCAACCAGCGCCTgcgc                                      <  1:625707/124‑1 (MQ=255)
                                                                                                                                           aCGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCGATTTGCAGGAGCAAAGCGTGAAACTCAACCAGCGCCTGCGCGATGCTGGGCGTGGCGATGAAACCCAg           <  2:1154488/150‑1 (MQ=255)
                                                                                                                                           aCGCTTGTGGCGGACCTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCGATTTGCAGGAGCAAAGCGTGAAACTCAACCAGCGCCTGCGCGATGCTGGGCGTGGCGATGAAACCCAg           <  2:892547/150‑1 (MQ=255)
                                                                                                                                                 gtgGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCGATTTGCAGGAGCAAAGCGTGAAACTCAACCAGCGCCTGCGCGATGCTGGGCGTGGCGATGAAACCCAGGTCAct     <  1:167128/150‑1 (MQ=255)
                                                                                                                                                    gCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCGATTTGCAGGAGCAAAGCGTGAAACTCAACCAGCGCCTGCGCGATGCTGGGCGTGGCGATGAAACCCAGGTCActcgc  <  1:3671/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GGTGCCGGTAGCCAACATCGGTGAGGCCATCATCAGTGCTTCGTACCAGTTCGACCTGTGGGGCACCTTCAAGCGTGGTACCGAGGCTGCCAAGGCAAATGCCGACGCGGTGCAGGCCGCTGCCGACACTGCCCGCATCACGCTTGTGGCGGACGTGGTCAAGGCTTATACCCAGGTGTGCTCGGCCAACGAGGAATACCACATTGCCCGTGAGTCCCTCGATTTGCAGGAGCAAAGCGTGAAACTCAACCAGCGCCTGCGCGATGCTGGGCGTGGCGATGAAACCCAGGTCACTCGC  >  NC_002947/230714‑231011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑